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    Microfluidic devices and methods
    7.
    发明授权
    Microfluidic devices and methods 有权
    微流体装置和方法

    公开(公告)号：US08389960B2

    公开(公告)日：2013-03-05

    申请号：US13295028

    申请日：2011-11-11

    申请人： Martin Pieprzyk ,  Geoff Facer ,  Timothy Woudenberg ,  Brian Fowler

    发明人： Martin Pieprzyk ,  Geoff Facer ,  Timothy Woudenberg ,  Brian Fowler

    IPC分类号： G01N21/64

    CPC分类号： G01N1/28 ,  B01L3/502738 ,  B01L7/52 ,  B01L2200/16 ,  B01L2300/0867 ,  B01L2300/0874 ,  B01L2300/123 ,  B01L2400/0481 ,  B01L2400/0655 ,  B33Y80/00 ,  C12Q1/686 ,  G01N21/05 ,  G01N21/6452 ,  G01N2021/0346 ,  Y10T137/7793 ,  Y10T436/2575

    摘要： Embodiments of the present invention provide improved microfluidic devices and related apparatus, systems, and methods. Methods are provided for reducing mixing times during use of microfluidic devices. Microfluidic devices and related methods of manufacturing are provided with increased manufacturing yield rates. Improved apparatus and related systems are provided for supplying controlled pressure to microfluidic devices. Methods and related microfluidic devices are provided for reducing dehydration of microfluidic devices during use. Microfluidic devices and related methods are provided with improved sample to reagent mixture ratio control. Microfluidic devices and systems are provided with improved resistance to compression fixture pressure induced failures. Methods and systems for conducting temperature controlled reactions using microfluidic devices are provided that reduce condensation levels within the microfluidic device. Methods and systems are provided for improved fluorescent imaging of microfluidic devices.

    摘要翻译： 本发明的实施例提供了改进的微流体装置和相关装置，系统和方法。 提供了减少使用微流体装置期间的混合时间的方法。 微流控装置和相关的制造方法具有提高的制造产率。 提供改进的装置和相关系统用于向微流体装置提供受控的压力。 提供了方法和相关的微流体装置，用于在使用过程中减少微流体装置的脱水。 微流控装置和相关方法提供了改进的样品与试剂混合比控制。 微流体装置和系统具有改进的抗压缩夹具压力引起的故障的阻力。 提供了使用微流体装置进行温度控制反应的方法和系统，其减少微流体装置内的冷凝水平。 提供了用于改进微流体装置的荧光成像的方法和系统。

    Method and System for Phase-Locked Sequencing
    10.
    发明申请
    Method and System for Phase-Locked Sequencing 审中-公开
    锁相排序方法与系统

    公开(公告)号：US20070154921A1

    公开(公告)日：2007-07-05

    申请号：US11611339

    申请日：2006-12-15

    申请人： Timothy Woudenberg ,  Meng Taing

    发明人： Timothy Woudenberg ,  Meng Taing

    IPC分类号： C12Q1/68 ,  C12M1/34 ,  G01J3/42

    CPC分类号： G01N21/6445 ,  C12Q1/6869 ,  G01N21/6428 ,  G01N21/6452 ,  G01N21/6458 ,  G01N2021/6432 ,  C12Q2527/113 ,  C12Q2525/186 ,  C12Q2523/319

    摘要： System and methods according to exemplary embodiments of the present disclosure utilize a sample holder configured to hold at least one confined single-molecule analyte in a solution of labeled nucleotide bases. Each single-molecule analyte has a single template nucleic acid molecule, an oligonucleotide primer, and/or a single nucleic acid polymerizing enzyme. A least one light source is used to illuminate a detection volume around each confined analyte, and a pulsed source sends a pulsed radiation to the at least one detection volume. The timing of incorporation events at the analytes are controlled by the pulsed radiation, and when multiple analytes are provided on the sample holder, the incorporation events at the analytes can be phase locked and synchronized using the pulsed radiation.

    摘要翻译： 根据本公开的示例性实施方案的系统和方法使用样品保持器，其被配置为将至少一个限制的单分子分析物保持在标记的核苷酸碱基的溶液中。 每个单分子分析物具有单个模板核酸分子，寡核苷酸引物和/或单个核酸聚合酶。 至少一个光源用于照亮围绕每个受限分析物的检测体积，并且脉冲源将脉冲辐射发送到至少一个检测体积。 分析物上的掺入事件的时间由脉冲辐射控制，当样品架上提供多个分析物时，分析物上的结合事件可以使用脉冲辐射进行锁相和同步。