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36 条记录 (耗时 0.021 秒)

    Method for producing amide compounds
    1.
    发明授权
    Method for producing amide compounds 失效
    酰胺化合物的制备方法

    公开(公告)号：US06900037B2

    公开(公告)日：2005-05-31

    申请号：US10172569

    申请日：2002-06-13

    申请人： Tohru Nagasawa ,  Akinobu Matsuyama

    发明人： Tohru Nagasawa ,  Akinobu Matsuyama

    IPC分类号： C12N1/21 ,  C12P13/02 ,  C12R1/01 ,  C12P7/40 ,  C12P41/00

    CPC分类号： C12P13/02

    摘要： The present invention provides a method for producing amide compounds using hydroxyl nitrile compounds and microorganisms and/or enzymatically active material having cyanide-resistant nitrile hydratase activity, e.g., Rhodococcus equi XL-l. Furthermore, the amide compounds can be produced while the enzymatic activity of this microorganism can be stably maintained during the reaction.

    摘要翻译： 本发明提供了使用羟基腈化合物和具有氰化物抗性腈水合酶活性的微生物和/或酶活性物质如红景天菌XL-1制备酰胺化合物的方法。 此外，可以在反应期间稳定地保持该微生物的酶活性的同时产生酰胺化合物。

    Processes for production of optically active quinuclidinol
    2.
    发明授权
    Processes for production of optically active quinuclidinol 失效
    光学活性奎宁环醇的制备方法

    公开(公告)号：US5888804A

    公开(公告)日：1999-03-30

    申请号：US034284

    申请日：1998-03-04

    申请人： Akinobu Matsuyama ,  Takeshi Hamatani

    发明人： Akinobu Matsuyama ,  Takeshi Hamatani

    IPC分类号： C12P17/12 ,  C12P17/18 ,  C12R1/06 ,  C12R1/37 ,  C12R1/38 ,  C12R1/645 ,  C12R1/72 ,  C12R1/78 ,  C07C00/00

    CPC分类号： C12P17/182 ,  Y10S435/83 ,  Y10S435/874

    摘要： The present invention provide processes for producing an optically active quinuclidinol from a quinuclidinone using an asymmetric reduction by a microorganism and enzyme with commercial advantages in simple and easy manner. In the present invention, permit a microorganism or preparation thereof to act on a quinuclidinone (3-quinuclidinone), and recover or harvest an optically active quinuclidinol produced (3-quinuclidinol). The microorganisms capable of producing an (R)-3-quinuclidinol from a 3-quinuclidinone include the genus Nakazawaea, the genus Candida and the genus Proteus. The microorganisms capable of producing an (S)-3-quinuclidinol from a 3-quinuclidinone include the genus Arthrobacter, the genus Pseudomonas and the genus Rhodosporidium.

    摘要翻译： 本发明提供了使用微生物和酶的不对称还原，以简便易行的商业优点从奎宁环酮生产光学活性奎宁环醇的方法。 在本发明中，允许微生物或其制备物作用于奎宁环酮（3-奎宁环酮），并回收或收获所产生的光学活性奎宁环醇（3-奎宁环醇）。 能够从3-奎宁环酮生成（R）-3-奎宁环醇的微生物包括中爪哇属（Nakazawaea），假丝酵母​​属（Candida）和变形菌属（Proteus）。 能够从3-奎宁环酮生成（S）-3-奎宁环醇的微生物包括节杆菌属，假单胞菌属和红球菌属。

    Heat-stable D-aminoacylase
    4.
    发明授权
    Heat-stable D-aminoacylase 失效
    热稳定的D-氨基酸酰基酶

    公开(公告)号：US06902915B2

    公开(公告)日：2005-06-07

    申请号：US10361509

    申请日：2003-02-07

    申请人： Shinji Tokuyama ,  Akinobu Matsuyama

    发明人： Shinji Tokuyama ,  Akinobu Matsuyama

    IPC分类号： C12N15/09 ,  C12N1/20 ,  C12N1/21 ,  C12N9/80 ,  C12N15/55 ,  C12P13/04 ,  C12P13/06 ,  C12P13/12 ,  C12P13/20 ,  C12P13/22 ,  C12R1/465 ,  C12N9/78 ,  C12P13/08

    CPC分类号： C12R1/465 ,  C12N9/80 ,  C12P13/04

    摘要： The present invention provides a novel D-aminoacylase, as well as method for producing a D-amino acid using the same. In order to achieve the above objective, the present inventors have succeeded in purifying heat-stable D-aminoacylase from microorganisms belonging to the genus Streptomyces by combining various purification methods. Furthermore, the present inventors found that the purified heat-stable D-aminoacylase is useful in industrial production of D-amino acids. By utilizing the heat-stable D-aminoacylase, it is possible to readily and efficiently produce the corresponding D-amino acids from N-acetyl-DL-amino acids (for example, N-acetyl-DL-methionine, N-acetyl-DL-valine, N-acetyl-DL-tryptophan, N-acetyl-DL-phenylalanine, N-acetyl-DL-alanine, N-acetyl-DL-leucine, and so on).

    摘要翻译： 本发明提供了一种新的D-氨基酸酰基酶，以及使用其制备D-氨基酸的方法。 为了实现上述目的，本发明人通过组合各种纯化方法成功地从属于链霉菌属的微生物纯化热稳定的D-氨基酰化酶。 此外，本发明人发现纯化的热稳定性D-氨基酸酰基酶可用于工业生产D-氨基酸。 通过利用热稳定的D-氨基酸酰化酶，可以容易且有效地从N-乙酰基-DL-氨基酸（例如，N-乙酰基-DL-甲硫氨酸，N-乙酰基-DL- 缬氨酸，N-乙酰基-DL-色氨酸，N-乙酰基-DL-苯丙氨酸，N-乙酰基-DL-丙氨酸，N-乙酰基-DL-亮氨酸等）。

    Process for enriching an �R,S!-1,2-epoxide in one enantiomer by using microbes to convert one enantiomer to the other or to preferentially open the epoxide ring
    7.
    发明授权
    Process for enriching an �R,S!-1,2-epoxide in one enantiomer by using microbes to convert one enantiomer to the other or to preferentially open the epoxide ring 失效
    通过使用微生物将一种对映异构体转化为另一对映异构体或优先开环环氧环来富集一种对映异构体中的[R，S]

    公开(公告)号：US5672504A

    公开(公告)日：1997-09-30

    申请号：US547902

    申请日：1995-10-25

    申请人： Akinobu Matsuyama ,  Yoshinori Kobayashi

    发明人： Akinobu Matsuyama ,  Yoshinori Kobayashi

    IPC分类号： C07D303/08 ,  C12P17/02 ,  C12P41/00

    CPC分类号： C07D303/08 ,  C12P17/02 ,  C12P41/00 ,  Y10S435/823 ,  Y10S435/832 ,  Y10S435/834 ,  Y10S435/839 ,  Y10S435/849 ,  Y10S435/875 ,  Y10S435/877 ,  Y10S435/881 ,  Y10S435/917 ,  Y10S435/918 ,  Y10S435/92

    摘要： A microorganism or a preparation thereof is permitted to act on a mixture of enantiomers of an epoxide such as 3-chlorostyrene oxide and the product optically active epoxide is recovered. The microorganism able to produce an optically active (S)-epoxide from the mixture of enantiomers of the epoxide include, for example, a microorganism strain belonging to the genus Candida, the genus Rhodosporidium, the genus Rhodococcus and the genus Nosardioides. Examples of the microorganism capable of producing an optically active (R)-epoxide from said mixture include a microorganism strain belonging to the genus Trichosporon, the genus Geotrichum, the genus Corynebacterium, the genus Micrococcus and the genus Brevibacterium. The objective optically active epoxide can efficiently be obtained with ease and simplicity from the corresponding mixture of enantiomers of the epoxide.

    摘要翻译： 允许微生物或其制剂作用于环氧化物的对映异构体如3-氯苯乙烯氧化物的混合物，并回收产物光学活性环氧化物。 能够从环氧化物的对映异构体的混合物中产生光学活性的（S） - 环氧化物的微生物包括例如属于假丝酵母属，红球菌属，红球菌属和仙人掌属的微生物菌株。 能够从所述混合物生产光学活性（R） - 环氧化物的微生物的实例包括属于Trichosporon属，Geotrichum属，棒状杆菌属，Micrococcus属和短杆菌属的微生物菌株。 目的光学活性环氧化物可以从环氧化物的相应对映异构体的混合物中容易且简单地获得。

    NOVEL EXPRESSION VECTOR
    9.
    发明申请
    NOVEL EXPRESSION VECTOR 审中-公开
    新的表达向导

    公开(公告)号：US20120142050A1

    公开(公告)日：2012-06-07

    申请号：US13144176

    申请日：2010-01-13

    申请人： Eitaro Matsumura ,  Momoko Hamasaki ,  Akinobu Matsuyama

    发明人： Eitaro Matsumura ,  Momoko Hamasaki ,  Akinobu Matsuyama

    IPC分类号： C12N1/21 ,  C12P21/00 ,  C12P1/04 ,  C12N15/74

    CPC分类号： C12N15/74 ,  C12N9/0089 ,  C12N9/78 ,  C12Y115/01001 ,  C12Y305/05001

    摘要： The present inventors constructed a transformation system which uses as a host the Kocuria rhizophila NBRC 103217 strain which can maintain its cellular structure in various organic solvents and shows little protein leakage.As a result, by developing novel shuttle vectors derived from Kocuria varians NBRC 15358, the present inventors successfully induced the expression of a foreign protein in microorganisms belonging to the genus Kocuria which can maintain their cellular structure in non-aqueous systems and show little protein leakage.

    摘要翻译： 本发明人构建了一种转化系统，其使用可以保持其在各种有机溶剂中的细胞结构并且几乎没有蛋白质渗漏的Kocuria rhizophila NBRC 103217菌株作为宿主。 结果，通过开发由Kocuria变种NBRC 15358衍生的新的穿梭载体，本发明人成功诱导了属于Kocuria属的微生物中的外源蛋白的表达，其可以在非水系统中保持其细胞结构并且显示出很少的蛋白质渗漏 。

    Stereospecific alcohol dehydrogenase isolated from Candida parapsilosis, amino acid and DNA sequences therefor, and method of preparation thereof
    10.
    发明授权
    Stereospecific alcohol dehydrogenase isolated from Candida parapsilosis, amino acid and DNA sequences therefor, and method of preparation thereof 失效
    从念珠菌分离的立体特异性醇脱氢酶，氨基酸及其DNA序列及其制备方法

    公开(公告)号：US06255092B1

    公开(公告)日：2001-07-03

    申请号：US08855767

    申请日：1997-06-11

    申请人： Tomoko Kojima ,  Hiroaki Yamamoto ,  Naoki Kawada ,  Akinobu Matsuyama

    发明人： Tomoko Kojima ,  Hiroaki Yamamoto ,  Naoki Kawada ,  Akinobu Matsuyama

    IPC分类号： C12N904

    CPC分类号： C12N9/0006 ,  C12P7/04 ,  C12P7/24 ,  C12P41/002

    摘要： The present invention provides a novel secondary alcohol dehydrogenase useful for the synthesis of optically active alcohol and DNA encoding said enzyme. A microorganism belonging to genus Candida was found to produce a novel secondary alcohol dehydrogenase with a high stereochemical specificity. Using said enzyme, optically active alcohols were prepared, and by cloning of DNA encoding said enzyme, the base sequence of said DNA was determined. By providing a novel secondary alcohol dehydrogenase with a high stereochemical specificity and the gene encoding said enzyme, an efficient production of optically active alcohols became possible.

    摘要翻译： 本发明提供了可用于合成光学活性醇和编码所述酶的DNA的新型仲醇脱氢酶。 发现属于念珠菌属的微生物产生具有高立体化学特异性的新型仲醇脱氢酶。 使用所述酶制备光学活性醇，通过克隆编码所述酶的DNA，测定所述DNA的碱基序列。 通过提供具有高立体化学特异性的新型仲醇脱氢酶和编码所述酶的基因，可以有效地制备光学活性醇。