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公开(公告)号：US07803907B2
公开(公告)日：2010-09-28
申请号：US11155989
申请日：2005-06-17
申请人： Willem P. C. Stemmer , D. Victor Perlroth , Sanjeev Satyal , Benjamin M. Alba , Alice Bakker , Amy N. Duguay , Qiang Liu , Joshua Silverman , Richard Smith , Candace Swimmer
发明人： Willem P. C. Stemmer , D. Victor Perlroth , Sanjeev Satyal , Benjamin M. Alba , Alice Bakker , Amy N. Duguay , Qiang Liu , Joshua Silverman , Richard Smith , Candace Swimmer
CPC分类号： C07K14/47 , A61K38/00 , C07K14/705 , G01N33/566 , G01N33/574 , G01N2333/4753 , G01N2333/82 , G01N2500/00
摘要： Polypeptides comprising monomer domains that bind to c-MET, or portions thereof, are provided.
摘要翻译： 提供了包含与c-MET结合的单体结构域的多肽或其部分。
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公开(公告)号：US07968681B2
公开(公告)日：2011-06-28
申请号：US12761346
申请日：2010-04-15
申请人： Willem P. C. Stemmer , D. Victor Perlroth , Sanjeev Satyal , Benjamin M. Alba , Alice Bakker , Amy N. Duguay , Qiang Lui , Joshua Silverman , Richard Smith , Candace Swimmer
发明人： Willem P. C. Stemmer , D. Victor Perlroth , Sanjeev Satyal , Benjamin M. Alba , Alice Bakker , Amy N. Duguay , Qiang Lui , Joshua Silverman , Richard Smith , Candace Swimmer
CPC分类号： C07K14/47 , A61K38/00 , C07K14/705 , G01N33/566 , G01N33/574 , G01N2333/4753 , G01N2333/82 , G01N2500/00
摘要： Polypeptides comprising monomer domains that bind to c-MET, or portions thereof, are provided.
摘要翻译： 提供了包含与c-MET结合的单体结构域的多肽或其部分。
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公开(公告)号：US08377681B2
公开(公告)日：2013-02-19
申请号：US13310332
申请日：2011-12-02
申请人： Stephen DelCardayre , Matthew B. Tobin , Willem P. C. Stemmer , Jon E. Ness , Jeremy S. Minshull , Phillip A. Patten , Venkiteswatan Mani Subramanian , Linda A. Castle , Claus M. Krebber , Steven H. Bass , Ying-Xin Zhang , Anthony R. Cox , Gjalt W. Huisman , Ling Yuan , Joseph A. Affholter
发明人： Stephen DelCardayre , Matthew B. Tobin , Willem P. C. Stemmer , Jon E. Ness , Jeremy S. Minshull , Phillip A. Patten , Venkiteswatan Mani Subramanian , Linda A. Castle , Claus M. Krebber , Steven H. Bass , Ying-Xin Zhang , Anthony R. Cox , Gjalt W. Huisman , Ling Yuan , Joseph A. Affholter
CPC分类号： C12N15/102 , C07K14/245 , C12N15/1027 , C12N15/8271 , C12N15/8273 , C12N15/8279 , C12P17/162 , C12Q1/68 , C12Q1/6811 , C12Q1/6827 , C12R1/465
摘要： The invention provides methods employing iterative cycles of recombination and selection/screening for evolution of whole cells and organisms toward acquisition of desired properties. Examples of such properties include enhanced recombinogenicity, genome copy number, and capacity for expression and/or secretion of proteins and secondary metabolites.
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公开(公告)号：US07957912B2
公开(公告)日：2011-06-07
申请号：US12557463
申请日：2009-09-10
申请人： Sergey A. Selifonov , Willem P. C. Stemmer , Claes Gustafsson , Matthew Tobin , Stephen del Cardayre , Phillip A. Patten , Jeremy Minshull , Lorraine J. Giver
发明人： Sergey A. Selifonov , Willem P. C. Stemmer , Claes Gustafsson , Matthew Tobin , Stephen del Cardayre , Phillip A. Patten , Jeremy Minshull , Lorraine J. Giver
摘要： In silico nucleic acid recombination methods, related integrated systems utilizing genetic operators and libraries made by in silico shuffling methods are provided. One disclosed method involves identifying and producing a polypeptide. The method may involve (a) receiving data identifying sequences of two or more parental polypeptides or parental nucleic acids that encode the polypeptides; (b) selecting one or more cross-over sites on the sequences thereby defining one or more recombinant polypeptides or recombinant nucleic acids; (c) selecting at least one of the recombinant polypeptides or recombinant nucleic acids; (d) recombining one or more oligonucleotides, at least one of which has a sequence matching some or all of that of the recombinant polypeptides or recombinant nucleic acids selected in (c); (e) selecting at least some of the recombined oligonucleotides produced in (d); and (f) producing a polypeptide encoded by the selected nucleic acid.
摘要翻译： 在硅胶核酸重组方法中，提供了利用遗传算子和通过计算机改组方法制备的文库的相关综合系统。 一种公开的方法涉及鉴定和产生多肽。 该方法可以包括（a）接收识别编码多肽的两个或多个亲本多肽或亲本核酸的序列的数据; （b）在序列上选择一个或多个交叉位点，从而限定一个或多个重组多肽或重组核酸; （c）选择至少一种重组多肽或重组核酸; （d）重组一种或多种寡核苷酸，其中至少一种具有与（c）中选择的重组多肽或重组核酸的部分或全部相匹配的序列; （e）选择（d）中产生的至少一些重组寡核苷酸; 和（f）产生由所选择的核酸编码的多肽。
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公开(公告)号：US07560622B2
公开(公告)日：2009-07-14
申请号：US09970004
申请日：2001-10-01
申请人： Willem P. C. Stemmer
发明人： Willem P. C. Stemmer
CPC分类号： C12N15/8213
摘要： The present invention provides methods of producing non-transgenic tissues or other agricultural products from a transgenic plant or other transgenic organism. Non-trausgenic organism parts, for example, are derived from transgenic organisms by expression of sequence-specific DNA cleaving enzymes that excise recombinant polynucleotide constructs from the organism's genome by cleaving the constructs at specific recognition sequences that flank the constructs.
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公开(公告)号：US06613514B2
公开(公告)日：2003-09-02
申请号：US09559671
申请日：2000-04-27
IPC分类号： C12Q168
CPC分类号： C12N15/1031 , A61K38/45 , A61K48/00 , C07K14/43595 , C07K14/545 , C07K14/56 , C07K16/00 , C07K2317/565 , C07K2317/622 , C12N7/00 , C12N9/0069 , C12N9/1007 , C12N9/16 , C12N9/86 , C12N15/10 , C12N15/1027 , C12N15/1034 , C12N15/1037 , C12N15/1058 , C12N15/52 , C12N15/64 , C12N15/67 , C12N2795/14043 , C12N2795/14143 , C12Q1/6811 , C12Q1/6853 , C12Q1/686 , C40B40/02 , C40B40/08 , C40B50/06 , G01N33/68 , G01N33/6845
摘要： Methods are provided for the evolution of proteins of industrial and pharmaceutical interest, including methods for effecting recombination and selection. Compositions produced by these methods are also disclosed.
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公开(公告)号：US06579678B1
公开(公告)日：2003-06-17
申请号：US09693350
申请日：2000-10-20
IPC分类号： C12Q168
CPC分类号： C12N15/1031 , A61K38/45 , A61K48/00 , C07K14/43595 , C07K14/545 , C07K14/56 , C07K16/00 , C07K2317/565 , C07K2317/622 , C12N7/00 , C12N9/0069 , C12N9/1007 , C12N9/16 , C12N9/86 , C12N15/10 , C12N15/1027 , C12N15/1034 , C12N15/1037 , C12N15/1058 , C12N15/52 , C12N15/64 , C12N15/67 , C12N2795/14043 , C12N2795/14143 , C12Q1/6811 , C12Q1/6853 , C12Q1/686 , C40B40/02 , C40B40/08 , C40B50/06 , G01N33/68 , G01N33/6845
摘要： Methods are provided for the evolution of proteins of industrial and pharmaceutical interest, including methods for effecting recombination and selection. Compositions produced by these methods are also disclosed.
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公开(公告)号：US06576467B1
公开(公告)日：2003-06-10
申请号：US09718764
申请日：2000-11-21
申请人： Willem P. C. Stemmer
发明人： Willem P. C. Stemmer
IPC分类号： C12N1500
CPC分类号： C40B40/02 , C07K14/43595 , C07K14/545 , C07K16/00 , C07K2317/565 , C07K2317/622 , C12N9/2471 , C12N9/86 , C12N15/1027 , C12N15/1034 , C12N15/1037 , C12Q1/68 , C12Q1/6811 , C12Y302/01023 , C12Y305/02006 , C40B40/08 , C40B50/06
摘要： A method for DNA reassembly after random fragmentation, and its application to mutagenesis of nucleic acid sequences by in vitro or in vivo recombination is described. In particular, a method for the production of nucleic acid fragments or polynucleotides encoding mutant proteins is described. The present invention also relates to a method of repeated cycles of mutagenesis, shuffling and selection which allow for the directed molecular evolution in vitro or in vivo of proteins.
摘要翻译： 描述了随机断裂后DNA重组的方法及其通过体外或体内重组诱变核酸序列的应用。 特别地，描述了用于生产编码突变蛋白的核酸片段或多核苷酸的方法。 本发明还涉及一种反复循环诱变，洗牌和选择的方法，其允许蛋白质的体外或体内的定向分子进化。
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公开(公告)号：US06506603B1
公开(公告)日：2003-01-14
申请号：US09717391
申请日：2000-11-18
申请人： Willem P. C. Stemmer
发明人： Willem P. C. Stemmer
IPC分类号： C12N1500
CPC分类号： C12N15/1058 , C07K14/43595 , C07K14/545 , C07K16/00 , C07K2317/565 , C07K2317/622 , C12N9/2471 , C12N9/86 , C12N15/1027 , C12N15/1037 , C12N15/52 , C12N15/64 , C12Q1/68 , C12Q1/6811 , C12Y302/01023 , C40B40/02 , Y10S435/876
摘要： A method for DNA reassembly after random fragmentation, and its application to mutagenesis of nucleic acid sequences by in vitro or in vivo recombination is described. In particular, a method for the production of nucleic acid fragments or polynucleotides encoding mutant proteins is described. The present invention also relates to a method of repeated cycles of mutagenesis, shuffling and selection which allow for the directed molecular evolution in vitro or in vivo of proteins.
摘要翻译： 描述了随机断裂后DNA重组的方法及其通过体外或体内重组诱变核酸序列的应用。 特别地，描述了用于生产编码突变蛋白的核酸片段或多核苷酸的方法。 本发明还涉及一种反复循环诱变，洗牌和选择的方法，其允许蛋白质的体外或体内的定向分子进化。
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公开(公告)号：US06500617B1
公开(公告)日：2002-12-31
申请号：US09296886
申请日：1999-04-22
IPC分类号： C12Q168
CPC分类号： C12N15/8285 , C07K14/325 , C07K2319/00 , C12N15/8286 , Y02A40/162 , Y02A40/164
摘要： This invention provides methods of obtaining pest resistance genes that are improved over naturally occurring genes for use in conferring upon plants resistance to pests. The methods involve the use of DNA shuffling of pest resistance genes to produce libraries of recombinant pest resistance genes, which are then screened to identify those that exhibit the improved property or properties of interest.
摘要翻译： 本发明提供了获得害虫抗性基因的方法，该基因比用于赋予植物抗病虫害性的天然存在的基因更好。 该方法涉及使用有害动物抗性基因的DNA改组来产生重组害虫抗性基因的文库，然后对其进行筛选以鉴定那些表现出改善的感兴趣的性质或性质的基因。
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