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    Method of uniformizing dna fragment contents and subtraction method
    5.
    发明申请
    Method of uniformizing dna fragment contents and subtraction method 审中-公开
    dna片段内容均匀化和减法方法

    公开(公告)号：US20050175997A1

    公开(公告)日：2005-08-11

    申请号：US10481228

    申请日：2002-06-19

    申请人： Yuichi Ono ,  Toshio Imai

    发明人： Yuichi Ono ,  Toshio Imai

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6809 ,  C12Q2531/113 ,  C12Q2525/191 ,  C12Q2525/131

    摘要： A method of normalizing contents of DNA fragments in a sample among respective DNA fragments, comprising: preparing DNA fragments in which adaptors each comprising an oligodeoxyribonucleotide are attached to both ends of each DNA fragment in the sample to form restriction enzyme recognition sites that do not exist in the DNA fragments in the sample, and at least a part between the restriction enzyme recognition sites at both ends, including the restriction enzyme recognition sites, is double-stranded; denaturing the prepared double-stranded DNA fragments; hybridizing the denatured DNA fragments under a condition that a part of the DNA fragments remains single-stranded; cleaving the hybridized double-stranded DNA fragments with a restriction enzyme having a cleavage site exclusively in the adaptors; and performing PCR using the obtained DNA fragments as templates and using a primer having a nucleotide sequence complementary to a nucleotide sequence of the adaptors before the cleavage; and a subtraction method comprising performing normalization by the method.

    摘要翻译： 使各DNA片段中的样品中DNA片段的含量正常化的方法，包括：制备DNA片段，其中每个包含寡脱氧核糖核苷酸的衔接子连接到样品中每个DNA片段的两端，以形成不存在的限制酶识别位点 在样品中的DNA片段中，两端的限制酶识别位点（包括限制酶识别位点）之间的至少一部分是双链的; 使制备的双链DNA片段变性; 在一部分DNA片段保持单链的条件下使变性DNA片段杂交; 用具有切割位点的限制酶切割杂交的双链DNA片段，仅在适配器中; 并使用所获得的DNA片段作为模板并使用具有与切割前的衔接子的核苷酸序列互补的核苷酸序列的引物进行PCR; 以及减法方法，包括通过该方法执行归一化。

    Multistage roots-type vacuum pump
    7.
    发明授权
    Multistage roots-type vacuum pump 有权
    多级根型真空泵

    公开(公告)号：US07491041B2

    公开(公告)日：2009-02-17

    申请号：US11509331

    申请日：2006-08-24

    申请人： Toshio Imai ,  Hideaki Itou ,  Masayuki Misaizu

    发明人： Toshio Imai ,  Hideaki Itou ,  Masayuki Misaizu

    IPC分类号： F04C2/00 ,  F03C2/00

    CPC分类号： F04C28/26 ,  F04C18/084 ,  F04C18/126 ,  F04C23/001 ,  F04C25/02

    摘要： The invention reduces power consumption and makes a rotor with ease. As illustrated in FIG. 2, a multistage roots pump (1) in the invention includes upstream rotors (R1a, R1b, R2a, R2b) having multiple teeth, supported by a pair of revolving shafts (A1, A2); and downstream rotors (R3a, R3b-R5a, R5b) having an identical number of teeth (31) with the upstream rotors, supported by revolving shafts (A1, A2). The discharge area formed by the outer periphery of the downstream rotors (R3a, R3b-R5a, R5b) and the inner periphewy of the pump chambers (P1-P5) is smaller than that of the upstream rotors (R1a, R1b, R2a, R2b).

    摘要翻译： 本发明降低功耗并使转子变得容易。 如图1所示。 如图2所示，本发明的多级根泵（1）包括具有多个齿的上游转子（R1a，R1b，R2a，R2b），由一对旋转轴（A1，A2）支撑; 和具有与上游转子相同数量的齿（31）的下游转子（R3a，R3b-R5a，R5b），由旋转轴（A1，A2）支撑。 由下游转子（R3a，R3b-R5a，R5b）的外周和泵室（P1-P5）的内周形成的排出面积小于上游转子（R1a，R1b，R2a，R2b ）。

    ILLUMINATION DEVICE
    8.
    发明申请
    ILLUMINATION DEVICE 有权
    照明装置

    公开(公告)号：US20070201217A1

    公开(公告)日：2007-08-30

    申请号：US11679674

    申请日：2007-02-27

    申请人： Toshio Imai

    发明人： Toshio Imai

    IPC分类号： G01D11/28

    CPC分类号： H01H13/023 ,  Y10S362/80

    摘要： An illumination device includes a control knob having a display portion, a tubular case combined with a back side of the control knob, a light guide plate attached to seal an upper opening of the tubular case, and LEDs disposed at a lower opening of the tubular case. Light emitted from the LEDs is applied to the display portion through the light guide plate. A plurality of retaining projections and a plurality of push-up projections are provided on an inner surface of the tubular case adjacent to the upper opening. An upper surface of an outer edge of the light guide plate is retained by lower ends of the retaining projections, and a lower surface of the outer edge is pushed up by upper ends of the push-up projections so that the light guide plate is held inside the tubular case while being bent upwardly.

    摘要翻译： 照明装置包括具有显示部分的控制旋钮，与控制旋钮的后侧相结合的管状外壳，连接以密封管状外壳的上部开口的导光板，以及设置在管状壳体的下部开口处的LED 案件。 从LED发射的光通过导光板施加到显示部分。 多个保持突起和多个上推突起设置在与上开口相邻的管状壳体的内表面上。 导光板的外缘的上表面由保持突起的下端保持，并且外缘的下表面被上推突起的上端向上推动，使得导光板被保持 在向上弯曲的同时在管状壳体内部。

    Method of aseptic filling
    9.
    发明申请
    Method of aseptic filling 审中-公开
    无菌灌装方法

    公开(公告)号：US20050199311A1

    公开(公告)日：2005-09-15

    申请号：US10517992

    申请日：2003-06-17

    申请人： Toshio Imai ,  Naoyuki Kojima

    发明人： Toshio Imai ,  Naoyuki Kojima

    IPC分类号： B65B55/04 ,  A61L2/00 ,  A61L2/04 ,  A61L2/18 ,  B65B55/06 ,  B65B55/12 ,  B65B55/14 ,  B67C7/00 ,  B65B1/20

    CPC分类号： B65B55/14 ,  A61L2/18 ,  B67C7/0073

    摘要： Preliminarily sterilized contents are filed in a synthetic resin container, which has been subjected to rinsing and sterilization. In this instance, the contents are maintained at a filling temperature that is higher than normal temperature, but lower than a temperature expressing sterilization effect, so that the container is prevented from deformation, which took place inevitably when heating the aseptically filled contents.

    摘要翻译： 将预先灭菌的内容物放入经过漂洗和灭菌的合成树脂容器中。 在这种情况下，内容物保持在比正常温度高的填充温度，但低于表示灭菌效果的温度，从而防止容器在加热无菌填充的内容物时不可避免地发生变形。

    Electrostatic capacitance detecting device
    10.
    发明授权
    Electrostatic capacitance detecting device 失效
    静电电容检测装置

    公开(公告)号：US06448790B1

    公开(公告)日：2002-09-10

    申请号：US09695426

    申请日：2000-10-25

    申请人： Toshio Imai

    发明人： Toshio Imai

    IPC分类号： G01R2726

    CPC分类号： G06K9/0002

    摘要： Bias charge is injected from a bias power supply line 3 into a detecting electrode 11 constituting an electrostatic capacitance element between an object to be detected to accumulate charge in accordance with its electrostatic capacitance, and when the charge is distributed between the electrostatic capacitance element and a capacitance element 12 which has been reset to a reset potential, a holding voltage of the capacitance element 12 changes in accordance with the amount of charges accumulated in the detecting electrode 11, and thus a signal is outputted regarding the change in holding voltage as an amount of change in gate potential of a source follower amplifier element 13, thereby detecting the capacitance of the electrostatic capacitance element between the object to be detected and the detecting electrode 11.

    摘要翻译： 偏置电荷从偏置电源线3注入到构成检测对象物之间的静电电容元件的检测电极11中，以根据其静电电容积蓄电荷，并且当电荷分布在静电电容元件和 已经复位到复位电位的电容元件12，电容元件12的保持电压根据在检测电极11中累积的电荷量而变化，从而输出关于保持电压的变化的信号作为量 源极跟随器放大器元件13的栅极电位的变化，从而检测待检测对象物和检测电极11之间的静电电容元件的电容。