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    Polyvalent display libraries
    1.
    发明授权
    Polyvalent display libraries 失效
    多价显示库

    公开(公告)号：US6057098A

    公开(公告)日：2000-05-02

    申请号：US832985

    申请日：1997-04-04

    申请人： Joe Buechler ,  Gunars Valkirs ,  Jeff Gray

    发明人： Joe Buechler ,  Gunars Valkirs ,  Jeff Gray

    IPC分类号： C07K16/00 ,  C12N15/10 ,  C12P21/04 ,  C40B40/02 ,  C12Q1/68 ,  C12N15/64 ,  G01N33/53

    CPC分类号： C40B40/02 ,  C07K16/00 ,  C12N15/1037 ,  C07K2319/00

    摘要： The invention is directed to inter alia two related but self-sufficient improvements in conventional display methods. The first improvement provides methods of enriching conventional display libraries for members displaying more than one copy of a polypeptide prior to affinity screening of such libraries with a target of interest. These methods can achieve diverse populations in which the vast majority of members retaining full-length coding sequences encode polypeptides having specific affinity for the target. In a second aspect, the invention provides methods of subcloning nucleic acids encoding displayed polypeptides of enriched libraries from a display vector to an expression vector without the need for clonal isolation of individual members. These methods result in polyclonal libraries of antibodies and other polypeptides for use, e.g., as diagnostic or therapeutic reagents.

    摘要翻译： 本发明特别涉及传统显示方法中的两个相关但自给自足的改进。 第一个改进提供了丰富常规显示库的方法，用于在用感兴趣的靶标亲和筛选此类文库之前，显示多于一个拷贝的成员的成员。 这些方法可以实现多种群，其中保留全长编码序列的绝大多数成员编码对靶具有特异性亲和力的多肽。 在第二方面，本发明提供将编码显示多肽的多肽的核酸从显示载体亚克隆到表达载体的方法，而不需要单个成员的克隆分离。 这些方法导致抗体和其他多肽的多克隆文库用于例如诊断或治疗试剂。

    Chimeric polyclonal antibodies
    3.
    发明申请
    Chimeric polyclonal antibodies 审中-公开

    公开(公告)号：US20030104477A1

    公开(公告)日：2003-06-05

    申请号：US10193960

    申请日：2002-07-12

    申请人： Biosite Diagnostics, Inc.

    发明人： Joe Buechler ,  Gunars Valkirs ,  Jeff Gray

    IPC分类号： G01N033/53 ,  G01N033/543 ,  C07K016/44

    CPC分类号： C40B40/02 ,  C07K16/00 ,  C07K16/1282 ,  C07K2319/00 ,  C12N15/1037 ,  G01N33/54326 ,  G01N2333/33 ,  G01N2400/50

    摘要： The invention is directed to production of chimeric antibodies using display screening methods. The invention is based in part on two related but self-sufficient improvements in conventional display methods. The first improvement provides methods of enriching conventional display libraries for members displaying more than one copy of a polypeptide prior to affinity screening of such libraries with a target of interest. These methods can achieve diverse populations in which the vast majority of members retaining full-length coding sequences encode polypeptides having specific affinity for the target. In a second aspect, the invention provides methods of subcloning nucleic acids encoding displayed polypeptides of enriched libraries from a display vector to an expression vector without the need for clonal isolation of individual members. These methods can be used to produce polyclonal libraries of chimeric antibodies for use, e.g., as diagnostic or therapeutic reagents.

    Crosstalk inhibitors and their uses
    5.
    发明授权
    Crosstalk inhibitors and their uses 失效
    串扰抑制剂及其用途

    公开(公告)号：US5525524A

    公开(公告)日：1996-06-11

    申请号：US101677

    申请日：1993-08-03

    申请人： Kenneth F. Buechler ,  Richard R. Anderson ,  Theodore T. Lee ,  Gunars E. Valkirs

    发明人： Kenneth F. Buechler ,  Richard R. Anderson ,  Theodore T. Lee ,  Gunars E. Valkirs

    IPC分类号： C07D207/40 ,  C07D207/416 ,  C07K14/00 ,  C07K14/76 ,  C12N9/16 ,  G01N33/53 ,  G01N33/531 ,  G01N33/543 ,  G01N33/94 ,  G01N33/74

    CPC分类号： C07D207/416 ,  C12N9/16 ,  G01N33/5306 ,  G01N33/54353 ,  G01N33/94 ,  Y10S435/962 ,  Y10S436/816 ,  Y10S436/817 ,  Y10S436/822

    摘要： Assay for detecting the amount or presence of target ligand in a sample. The assay includes a ligand analogue conjugate having a linkage site and a binding site, a ligand receptor, and a sample. The assay includes the steps of providing at least one crosstalk inhibitor. This inhibitor, under assay conditions, competes with the linkage site of the ligand analogue conjugate for binding to the ligand receptor, and does not compete with the binding site of the ligand analogue conjugate for binding to the ligand receptor. In the invention, the assay is performed for the target ligand in the presence of a sufficient amount of the crosstalk inhibitor to reduce the amount of binding of the linkage site of the ligand analogue conjugate to the ligand receptor. The invention also features a method for identifying crosstalk inhibitors, and the crosstalk inhibitors themselves.

    摘要翻译： 用于检测样品中靶配体的量或存在的测定。 该测定包括具有连接位点和结合位点，配体受体和样品的配体类似物缀合物。 该测定包括提供至少一种串扰抑制剂的步骤。 在测定条件下，该抑制剂与配体类似物缀合物的连接位点竞争结合配体受体，并且不与配体类似物缀合物的结合位点竞争结合配体受体。 在本发明中，在足够量的串扰抑制剂的存在下，对靶配体进行测定以减少配体类似物缀合物与配体受体的连接位点的结合量。 本发明还具有用于识别串扰抑制剂和串扰抑制剂本身的方法。

    Antibodies to complexes of ligand receptors and ligands and their utility in ligand-receptor assays
    7.
    发明授权
    Antibodies to complexes of ligand receptors and ligands and their utility in ligand-receptor assays 失效
    配体受体和配体复合物的抗体及其在配体 - 受体测定中的应用

    公开(公告)号：US5985579A

    公开(公告)日：1999-11-16

    申请号：US458901

    申请日：1995-06-02

    申请人： Kenneth F. Buechler ,  Gunars E. Valkirs

    发明人： Kenneth F. Buechler ,  Gunars E. Valkirs

    IPC分类号： G01N33/53 ,  G01N33/538 ,  G01N33/541 ,  G01N33/543 ,  G01N33/564 ,  G01N33/566

    CPC分类号： G01N33/54306 ,  G01N33/5306 ,  G01N33/541 ,  G01N33/54393 ,  Y10S435/97 ,  Y10S435/971 ,  Y10S435/975 ,  Y10S436/807

    摘要： Methods and test devices for detecting the presence or amount of target ligand in non-competitive sandwich ligand-receptor assay processes. Antibodies which bind to the complex of ligand receptor and target ligand but do not bind significantly to the ligand receptor and which bind the target ligand with substantially less affinity than the complex are taught and their uses described. These assays can be used to eliminate the "hook" effect in non-competitive sandwich assays. Furthermore, the antibodies are selected and assay methods described so that, as a result of the assay process, no detectable response is observed due to the binding of antibody and ligand receptor in the absence of target ligand.

    摘要翻译： 用于检测非竞争性三明治配体 - 受体测定方法中靶配体的存在或量的方法和测试装置。 教导了与配体受体和靶配体的配合物结合但不与配体受体显着结合并以与复合物相近的亲和力结合靶配体的抗体，并描述了它们的用途。 这些测定可用于消除非竞争性夹心测定中的“钩”效应。 此外，选择抗体并描述测定方法，使得作为测定方法的结果，由于在不存在靶配体的情况下抗体和配体受体的结合，观察不到可检测的反应。

    Assays and kits for detecting analytes in the presence of cross-reacting substances
    8.
    发明授权
    Assays and kits for detecting analytes in the presence of cross-reacting substances 失效
    在交叉反应物质存在下检测分析物的测定和试剂盒

    公开(公告)号：US5914241A

    公开(公告)日：1999-06-22

    申请号：US006341

    申请日：1993-01-19

    申请人： Gunars E. Valkirs

    发明人： Gunars E. Valkirs

    IPC分类号： G01N33/543 ,  G01N33/536 ,  G01N33/573

    CPC分类号： G01N33/54306 ,  Y10S435/971

    摘要： An immunoassay is provided which is selective for an analyte over immunologically related substances which may be present in a sample to be tested. The presence of the analyte is detected using a first binding substance, typically an antibody, in the presence of a second binding substance, typically another antibody. The first binding substance recognizes an epitope which is characteristic of the analyte and cross-reacts with a related epitope on the cross-binding substance. The second binding substance preferentially binds the common epitope on the cross-binding substance, thus reducing non-specific binding of the first binding substance.

    摘要翻译： 提供免疫测定法，其对于可能存在于待测试样品中的免疫相关物质的分析物是选择性的。 在第二结合物质（通常是另一种抗体）的存在下，使用第一结合物质（通常为抗体）检测分析物的存在。 第一结合物质识别分析物特征的表位，并与交联结合物质上的相关表位交叉反应。 第二结合物质优先结合交联结合物质上的共同表位，从而降低第一结合物质的非特异性结合。

    Tetrahydrocannabinol derivatives and protein and polypeptide tetrahydrocannabinol derivative conjugates and labels
    10.
    发明授权
    Tetrahydrocannabinol derivatives and protein and polypeptide tetrahydrocannabinol derivative conjugates and labels 失效
    四氢大麻酚衍生物和蛋白质和多肽四氢大麻酚衍生物缀合物和标签

    公开(公告)号：US5237057A

    公开(公告)日：1993-08-17

    申请号：US864106

    申请日：1992-04-06

    申请人： Kenneth F. Buechler ,  Si S. Moi

    发明人： Kenneth F. Buechler ,  Si S. Moi

    IPC分类号： G01N33/53 ,  A61K39/385 ,  C07D311/80 ,  C07D409/12 ,  C07K1/113 ,  C07K14/00 ,  C07K16/44 ,  G01N33/566

    CPC分类号： C07D409/12 ,  Y10S435/964

    摘要： The present invention is directed to novel THC derivatives which are synthesized for the covalent attachment to antigens (proteins or polypeptides) for the preparation of antibodies or receptors to the THC metabolites. The resulting novel antigens may be used for the production of antibodies or receptors using standard methods. Once generated, the antibodies or receptors and the novel derivatives which are covalently attached to proteins, polypeptides or labels may be used in the immunoassay process.

    摘要翻译： 本发明涉及合成用于共价连接到抗原（蛋白质或多肽）以用于制备THC代谢物的抗体或受体的新型THC衍生物。 所得的新抗原可以用于使用标准方法生产抗体或受体。 一旦产生，共价连接到蛋白质，多肽或标记的抗体或受体和新型衍生物可用于免疫测定过程。