公开(公告)号：US20140024074A1

公开(公告)日：2014-01-23

申请号：US13699647

申请日：2011-06-06

Applicant: Leo L. Chan

Inventor： Leo L. Chan

IPC: C12Q1/06

CPC classification number: C12Q1/06 ,  C12Q1/04 ,  C12Q1/64 ,  G01N2333/39

Abstract: The invention generally relates to analyzing yeast viability. More particularly, the invention relates to efficient and effective methods and compositions for accessing and measuring viability and concentration of yeast cells.

Abstract translation: 本发明一般涉及分析酵母存活力。 更具体地，本发明涉及用于获取和测量酵母细胞的活力和浓度的有效和有效的方法和组合物。

公开(公告)号：US11911764B2

公开(公告)日：2024-02-27

申请号：US16963233

申请日：2019-02-21

Applicant: Nexcelom Bioscience LLC

Inventor： Peter Y. Li ,  Jean Qiu ,  Leo L. Chan ,  Justin R. Hascup ,  Timothy F. Smith ,  Todd R. Sobolewski ,  Nicholas N. Shaw

IPC: C12Q1/00 ,  B01L3/00 ,  G01N15/14 ,  G01N33/487 ,  G01N15/10

CPC classification number: B01L3/5085 ,  G01N15/147 ,  G01N33/4875 ,  B01L2200/10 ,  B01L2300/0654 ,  B01L2300/087 ,  B01L2300/0829 ,  G01N2015/1006 ,  G01N2015/1486

Abstract: The invention provides novel sample chambers, sample analysis units, multi-well plates, and imaging and analytical systems as well as methods for accurate, efficient and high-throughput imaging, measurement and analysis of diverse types of biological cells to obtain information such as cell count, cell size, cell concentration, cell sub-population, cell morphology, cell viability, etc.

公开(公告)号：US09329130B2

公开(公告)日：2016-05-03

申请号：US13519282

申请日：2011-01-11

Applicant: Leo L. Chan ,  Peter Li

Inventor： Leo L. Chan ,  Peter Li

IPC: G01N21/64 ,  G01N15/14 ,  G01N33/50 ,  G01N21/3581 ,  G01J5/10

CPC classification number: G01N21/6486 ,  G01J3/10 ,  G01J3/4406 ,  G01J5/10 ,  G01N15/1463 ,  G01N21/3581 ,  G01N21/64 ,  G01N21/6428 ,  G01N21/6456 ,  G01N21/6458 ,  G01N33/5005 ,  G01N2015/1486 ,  G01N2021/1736 ,  G01N2021/174 ,  G01N2021/6419 ,  G01N2021/6441 ,  G01N2021/6491 ,  G01N2201/0627

Abstract: The invention generally relates to analytical and monitoring systems useful for analyzing and measuring cells and biological samples. More particularly, the invention relates to systems and methods for imaging, measuring, counting, analyzing, and monitoring microscopic particles such as cells and biological molecules in solution samples.

Abstract translation: 本发明一般涉及用于分析和测量细胞和生物样品的分析和监测系统。 更具体地，本发明涉及用于在溶液样品中成像，测量，计数，分析和监测微观颗粒如细胞和生物分子的系统和方法。

公开(公告)号：US08928876B2

公开(公告)日：2015-01-06

申请号：US13519283

申请日：2011-01-19

Applicant: Leo L. Chan ,  Jean Qiu

Inventor： Leo L. Chan ,  Jean Qiu

IPC: G01N1/10 ,  G01N21/03 ,  B01L3/00 ,  B29C65/50 ,  B29C65/00 ,  G01N15/14 ,  G01N21/64 ,  B29L31/00 ,  B29C65/48

CPC classification number: B29C59/021 ,  B01L3/502707 ,  B01L2200/0689 ,  B01L2300/0877 ,  B01L2300/0887 ,  B29C65/4825 ,  B29C65/4835 ,  B29C65/5021 ,  B29C65/5057 ,  B29C66/1122 ,  B29C66/53461 ,  B29C66/71 ,  B29K2033/08 ,  B29K2063/00 ,  B29K2075/00 ,  B29L2031/756 ,  G01N15/14 ,  G01N15/1463 ,  G01N15/1484 ,  G01N21/03 ,  G01N21/6458 ,  G01N2015/1486 ,  G01N2201/02 ,  Y10T29/49826 ,  B29K2067/00 ,  B29K2069/00 ,  B29K2033/12 ,  B29K2025/06 ,  B29K2023/38

Abstract: The invention generally relates to analytical and monitoring systems useful for analyzing and measuring cells and biological sample. More particularly, the invention relates to a unique cell counting chamber, e.g., a thin gap fluidic cell chamber for both bright field and fluorescent imaging of bacteria or parasites, and methods for making the same.

Abstract translation: 本发明一般涉及用于分析和测量细胞和生物样品的分析和监测系统。 更具体地，本发明涉及一种独特的细胞计数室，例如用于细菌或寄生虫的亮场和荧光成像的薄间隙流体细胞室及其制备方法。

公开(公告)号：US20210069696A1

公开(公告)日：2021-03-11

申请号：US16963233

申请日：2019-02-21

Applicant: Nexcelom Bioscience LLC

Inventor： Peter Y. Li ,  Jean Qiu ,  Leo L. Chan ,  Justin R. Hascup ,  Timothy F. Smith ,  Todd R. Sobolewski ,  Nicholas N. Shaw

IPC: B01L3/00 ,  G01N33/487 ,  G01N15/14

Abstract: The invention provides novel sample chambers, sample analysis units, multi-well plates, and imaging and analytical systems as well as methods for accurate, efficient and high-throughput imaging, measurement and analysis of diverse types of biological cells to obtain information such as cell count, cell size, cell concentration, cell sub-population, cell morphology, cell viability, etc.

公开(公告)号：US09933415B2

公开(公告)日：2018-04-03

申请号：US14790482

申请日：2015-07-02

Applicant: NEXCELOM BIOSCIENCE LLC

Inventor： Leo L Chan ,  Jean Qiu ,  Peter Li ,  Kevin Flanagan ,  Timothy Smith

IPC: G06K9/00 ,  G01N33/50 ,  G06F19/26 ,  G01N21/64

CPC classification number: G01N33/5094 ,  G01N21/6428 ,  G01N21/6456 ,  G01N21/6486 ,  G06F19/26

Abstract: The invention generally relates to analytical and monitoring systems useful for analyzing and measuring cells and biological samples. More particularly, the invention provides systems and methods for internal calibration and focus reference for cytometry imaging.

公开(公告)号：US09342734B2

公开(公告)日：2016-05-17

申请号：US14452612

申请日：2014-08-06

Applicant: NEXCELOM BIOSCIENCE LLC

Inventor： Bo Lin ,  Peter Y. Li ,  Jean Qiu ,  Timothy Smith ,  Todd Sobolewski ,  Alnoor Pirani

IPC: G01N33/487 ,  G01N21/64 ,  G02B21/06 ,  G02B21/16 ,  G06K9/00 ,  G01N15/14 ,  G02B21/36 ,  G01N15/10

CPC classification number: G06K9/00134 ,  G01N15/1475 ,  G01N21/64 ,  G01N21/6458 ,  G01N33/487 ,  G01N2015/1006 ,  G01N2015/1486 ,  G01N2015/1493 ,  G01N2021/6439 ,  G01N2201/062 ,  G02B21/06 ,  G02B21/16 ,  G02B21/365 ,  Y10T436/101666 ,  Y10T436/143333

Abstract: The present invention generally relates to systems and methods for counting biomolecules or cells. In certain embodiments, the invention provides a cell counting or biomolecule counting system including: a covered chamber having a known height and configured to hold a suspension of biomolecules or cells in a sample; at least one fluorescent light source connected to at least one fluorescent light beam narrowing device; a bright-field light source connected to a bright-field light beam narrowing device; a microscope objective; a detection device; a fluorescent filter assembly to allow only excitation light to illuminate the sample and allow only emission light from the sample to be imaged by the detection device; and a movable light shutter to block bright-field light during fluorescent detection.

Abstract translation: 本发明一般涉及计数生物分子或细胞的系统和方法。 在某些实施方案中，本发明提供细胞计数或生物分子计数系统，其包括：具有已知高度并被构造成保持样品中生物分子或细胞悬浮液的被覆室; 至少一个荧光光源连接到至少一个荧光光束变窄装置; 连接到明场光束变窄装置的亮场光源; 显微镜目标; 检测装置; 荧光过滤器组件，仅允许激发光照射样品并且仅允许来自样品的发射光被检测装置成像; 以及可动光闸以在荧光检测期间阻挡亮场光。

公开(公告)号：US09186843B2

公开(公告)日：2015-11-17

申请号：US14587932

申请日：2014-12-31

Applicant: NEXCELOM BIOSCIENCE LLC

Inventor： Leo L. Chan ,  Jean Qiu

IPC: G01N1/10 ,  B29C59/02 ,  B01L3/00 ,  B29C65/50 ,  B29C65/00 ,  G01N15/14 ,  G01N21/64 ,  G01N21/03 ,  B29L31/00 ,  B29C65/48 ,  B29K33/00 ,  B29K63/00 ,  B29K75/00

CPC classification number: B29C59/021 ,  B01L3/502707 ,  B01L2200/0689 ,  B01L2300/0877 ,  B01L2300/0887 ,  B29C65/4825 ,  B29C65/4835 ,  B29C65/5021 ,  B29C65/5057 ,  B29C66/1122 ,  B29C66/53461 ,  B29C66/71 ,  B29K2033/08 ,  B29K2063/00 ,  B29K2075/00 ,  B29L2031/756 ,  G01N15/14 ,  G01N15/1463 ,  G01N15/1484 ,  G01N21/03 ,  G01N21/6458 ,  G01N2015/1486 ,  G01N2201/02 ,  Y10T29/49826 ,  B29K2067/00 ,  B29K2069/00 ,  B29K2033/12 ,  B29K2025/06 ,  B29K2023/38

Abstract: The invention generally relates to analytical and monitoring systems useful for analyzing and measuring cells and biological sample. More particularly, the invention relates to a unique cell counting chamber, e.g., a thin gap fluidic cell chamber for both bright field and fluorescent imaging of bacteria or parasites, and methods for making the same.

公开(公告)号：US08883491B2

公开(公告)日：2014-11-11

申请号：US12935973

申请日：2009-04-08

Applicant: Bo Lin ,  Peter Y. Li ,  Jean Qiu ,  Timothy Smith ,  Todd Sobolewski ,  Alnoor Pirani

Inventor： Bo Lin ,  Peter Y. Li ,  Jean Qiu ,  Timothy Smith ,  Todd Sobolewski ,  Alnoor Pirani

IPC: G01N21/00 ,  C12M1/34 ,  G01N21/76 ,  G01N15/14 ,  G01N21/64 ,  G01N15/10

CPC classification number: G06K9/00134 ,  G01N15/1475 ,  G01N21/64 ,  G01N21/6458 ,  G01N33/487 ,  G01N2015/1006 ,  G01N2015/1486 ,  G01N2015/1493 ,  G01N2021/6439 ,  G01N2201/062 ,  G02B21/06 ,  G02B21/16 ,  G02B21/365 ,  Y10T436/101666 ,  Y10T436/143333

Abstract: The present invention generally relates to systems and methods for counting biomolecules or cells. In certain embodiments, the invention provides a cell counting or biomolecule counting system including: a covered chamber having a known height and configured to hold a suspension of biomolecules or cells in a sample; at least one fluorescent light source connected to at least one fluorescent light beam narrowing device; a bright-field light source connected to a bright-field light beam narrowing device; a microscope objective; a detection device; a fluorescent filter assembly to allow only excitation light to illuminate the sample and allow only emission light from the sample to be imaged by the detection device; and a movable light shutter to block bright-field light during fluorescent detection.

Abstract translation: 本发明一般涉及计数生物分子或细胞的系统和方法。 在某些实施方案中，本发明提供细胞计数或生物分子计数系统，其包括：具有已知高度并被构造成保持样品中生物分子或细胞悬浮液的被覆室; 至少一个荧光光源连接到至少一个荧光光束变窄装置; 连接到明场光束变窄装置的亮场光源; 显微镜目标; 检测装置; 荧光过滤器组件，其仅允许激发光照射样品并且仅允许来自样品的发射光被检测装置成像; 以及可动光闸以在荧光检测期间阻挡亮场光。

公开(公告)号：US20130336567A1

公开(公告)日：2013-12-19

申请号：US13878618

申请日：2011-10-20

Applicant: Leo L. Chan ,  Jean Qiu ,  Peter Li ,  Kevin Flanagan ,  Timothy Smith

Inventor： Leo L. Chan ,  Jean Qiu ,  Peter Li ,  Kevin Flanagan ,  Timothy Smith

IPC: G06F19/26

CPC classification number: G01N33/5094 ,  G01N21/6428 ,  G01N21/6456 ,  G01N21/6486 ,  G06F19/26

Abstract: The invention generally relates to analytical and monitoring systems useful for analyzing and measuring cells and biological samples. More particularly, the invention provides systems and methods for internal calibration and focus reference for cytometry imaging.

Abstract translation: 本发明一般涉及用于分析和测量细胞和生物样品的分析和监测系统。 更具体地，本发明提供用于细胞计数成像的内部校准和聚焦参考的系统和方法。