公开(公告)号：US20170226459A1

公开(公告)日：2017-08-10

申请号：US15016753

申请日：2016-02-05

申请人： Wisconsin Alumni Research Foundation

发明人： Zhenqiang Ma ,  Yei Hwan Jung ,  Michael Phillips ,  David Gamm ,  Shaoqin Gong

IPC分类号： C12M1/12 ,  C12M3/00 ,  C12M1/00

CPC分类号： C12M25/14 ,  C12M21/08 ,  C12M23/20 ,  C12M23/26 ,  C12M23/30

摘要： Photoreceptor scaffolds and scaffold systems including the photoreceptor scaffolds are described herein. The scaffolds and scaffold systems can be used for transplantation of organized photoreceptor tissue, with or without RPE, which may improve grafted cell survival, integration, and functional visual rescue. Particularly, the photoreceptor scaffold is structured from a biocompatible film, patterned with an array of unique through-holes having a curvilinear cell receiver and at least one cell guide channel.

公开(公告)号：US20140234953A1

公开(公告)日：2014-08-21

申请号：US14153591

申请日：2014-01-13

申请人： THE GENERAL HOSPITAL CORPORATION ,  THE CHARLES STARK DRAPER LABORATORY

发明人： Joseph P. Vacanti ,  Young-Moon M. Shin ,  Jennifer Ogilvie ,  Alexander Sevy ,  Tomoyuki Maemura ,  Osamu Ishii ,  Mohammad R. Kaazempur-Mofrad ,  Jeffrey T. Borenstein ,  Kevin R. King ,  Chiao-Chun Wang ,  Eli Weinberg

IPC分类号： C12M3/00

CPC分类号： A61F2/062 ,  A61F2240/004 ,  A61F2240/005 ,  A61L27/3604 ,  A61L27/3804 ,  A61L27/3808 ,  A61L27/3891 ,  A61L27/3895 ,  A61L27/58 ,  A61L2300/64 ,  B33Y80/00 ,  C12M21/08 ,  C12M23/06 ,  C12M23/30 ,  C12M25/02 ,  C12N5/0062 ,  C12N5/0697 ,  C12N2533/12 ,  C12N2533/30 ,  Y10T29/49826

摘要： Methods and materials for making complex, living, vascularized tissues for organ and tissue replacement, especially complex and/or thick, structures, such as liver tissue is provided. Tissue lamina is made in a system comprising an apparatus having (a) a first mold or polymer scaffold, a semi-permeable membrane, and a second mold or polymer scaffold, wherein the semi-permeable membrane is disposed between the first and second molds or polymer scaffolds, wherein the first and second molds or polymer scaffolds have means defining microchannels positioned toward the semi-permeable membrane, wherein the first and second molds or polymer scaffolds are fastened together; and (b) animal cells. Methods for producing complex, three-dimensional tissues or organs from tissue lamina are also provided.

摘要翻译： 提供用于制备器官和组织置换的复杂的，活的，血管化的组织，特别是复杂和/或较厚的结构例如肝组织的方法和材料。 在包括具有（a）第一模具​​或聚合物支架，半透膜和第二模具或聚合物支架的装置的系统中制造组织层，其中半透膜设置在第一和第二模具之间或 聚合物支架，其中第一和第二模具或聚合物支架具有限定朝向半透膜的微通道的装置，其中第一和第二模具或聚合物支架紧固在一起; 和（b）动物细胞。 还提供了从组织层片制备复合的三维组织或器官的方法。

公开(公告)号：US20100267136A1

公开(公告)日：2010-10-21

申请号：US12785865

申请日：2010-05-24

申请人： Joseph P. Vacanti ,  Young-Moon Michael Shin ,  Jennifer Ogilvie ,  Alexander Sevy ,  Tomoyuki Maemura ,  Osamu Ishii ,  Mohammad Reza Kaazempur-Mofrad ,  Jeffrey T. Borenstein ,  Kevin R. King ,  Chiao-Chun Wang ,  Eli Weinberg

发明人： Joseph P. Vacanti ,  Young-Moon Michael Shin ,  Jennifer Ogilvie ,  Alexander Sevy ,  Tomoyuki Maemura ,  Osamu Ishii ,  Mohammad Reza Kaazempur-Mofrad ,  Jeffrey T. Borenstein ,  Kevin R. King ,  Chiao-Chun Wang ,  Eli Weinberg

IPC分类号： C12N5/071 ,  C12M1/12 ,  B23P19/04

CPC分类号： A61F2/062 ,  A61F2240/004 ,  A61F2240/005 ,  A61L27/3604 ,  A61L27/3804 ,  A61L27/3808 ,  A61L27/3891 ,  A61L27/3895 ,  A61L27/58 ,  A61L2300/64 ,  B33Y80/00 ,  C12M21/08 ,  C12M23/06 ,  C12M23/30 ,  C12M25/02 ,  C12N5/0062 ,  C12N5/0697 ,  C12N2533/12 ,  C12N2533/30 ,  Y10T29/49826

摘要： Methods and materials for making complex, living, vascularized tissues for organ and tissue replacement, especially complex and/or thick structures, such as liver tissue is provided. Tissue lamina is made in a system comprising an apparatus having (a) a first mold or polymer scaffold, a semi-permeable membrane, and a second mold or polymer scaffold, wherein the semi-permeable membrane is disposed between the first and second molds or polymer scaffolds, wherein the first and second molds or polymer scaffolds have means defining microchannels positioned toward the semi-permeable membrane, wherein the first and second molds or polymer scaffolds are fastened together; and (b) animal cells. Methods for producing complex, three-dimensional tissues or organs from tissue lamina are also provided.

摘要翻译： 提供了用于制造器官和组织置换的复杂的，活的，血管化的组织，特别是复杂和/或较厚的结构例如肝组织的方法和材料。 在包括具有（a）第一模具​​或聚合物支架，半透膜和第二模具或聚合物支架的装置的系统中制造组织层，其中半透膜设置在第一和第二模具之间或 聚合物支架，其中第一和第二模具或聚合物支架具有限定朝向半透膜的微通道的装置，其中第一和第二模具或聚合物支架紧固在一起; 和（b）动物细胞。 还提供了从组织层片制备复合的三维组织或器官的方法。

公开(公告)号：US08642336B2

公开(公告)日：2014-02-04

申请号：US12785865

申请日：2010-05-24

申请人： Joseph P. Vacanti ,  Young-Moon Michael Shin ,  Jennifer Ogilvie ,  Alexander Sevy ,  Tomoyuki Maemura ,  Osamu Ishii ,  Mohammad Reza Kaazempur-Mofrad ,  Jeffrey T. Borenstein ,  Kevin R. King ,  Chiao-Chun Wang ,  Eli Weinberg

发明人： Joseph P. Vacanti ,  Young-Moon Michael Shin ,  Jennifer Ogilvie ,  Alexander Sevy ,  Tomoyuki Maemura ,  Osamu Ishii ,  Mohammad Reza Kaazempur-Mofrad ,  Jeffrey T. Borenstein ,  Kevin R. King ,  Chiao-Chun Wang ,  Eli Weinberg

IPC分类号： C12N5/02

CPC分类号： A61F2/062 ,  A61F2240/004 ,  A61F2240/005 ,  A61L27/3604 ,  A61L27/3804 ,  A61L27/3808 ,  A61L27/3891 ,  A61L27/3895 ,  A61L27/58 ,  A61L2300/64 ,  B33Y80/00 ,  C12M21/08 ,  C12M23/06 ,  C12M23/30 ,  C12M25/02 ,  C12N5/0062 ,  C12N5/0697 ,  C12N2533/12 ,  C12N2533/30 ,  Y10T29/49826

摘要： Methods and materials for making complex, living, vascularized tissues for organ and tissue replacement, especially complex and/or thick structures, such as liver tissue is provided. Tissue lamina is made in a system comprising an apparatus having (a) a first mold or polymer scaffold, a semi-permeable membrane, and a second mold or polymer scaffold, wherein the semi-permeable membrane is disposed between the first and second molds or polymer scaffolds, wherein the first and second molds or polymer scaffolds have means defining microchannels positioned toward the semi-permeable membrane, wherein the first and second molds or polymer scaffolds are fastened together; and (b) animal cells. Methods for producing complex, three-dimensional tissues or organs from tissue lamina are also provided.

摘要翻译： 提供了用于制造器官和组织置换的复杂的，活的，血管化的组织，特别是复杂和/或较厚的结构例如肝组织的方法和材料。 在包括具有（a）第一模具​​或聚合物支架，半透膜和第二模具或聚合物支架的装置的系统中制造组织层，其中半透膜设置在第一和第二模具之间或 聚合物支架，其中第一和第二模具或聚合物支架具有限定朝向半透膜的微通道的装置，其中第一和第二模具或聚合物支架紧固在一起; 和（b）动物细胞。 还提供了从组织层片制备复合的三维组织或器官的方法。

公开(公告)号：US20130260465A1

公开(公告)日：2013-10-03

申请号：US13905487

申请日：2013-05-30

申请人： Dawn R. Applegate

发明人： Dawn R. Applegate

IPC分类号： C12N5/00

CPC分类号： C12N5/0068 ,  C12M23/12 ,  C12M23/30 ,  C12M25/04 ,  C12M25/14

摘要： A microplate is provided herein having a plate body with at least one well formed therein, the well having a first open end, a second end, an aperture being formed in the second end, and a side wall extending between the first end and the second end. The microplate further has a permeable membrane extending at least partially across the aperture formed in the second end. A microplate is provided with a permeable membrane which allows not only for removal of certain solutes from a solution (high or low molecular weight solutes), but also allows for separation of macromolecular mixtures and degradation of the membrane. The microplate is also provided with an integrated top assembly or integrated inserts. Also provided herein are methods of culturing systems in the microplates described.

摘要翻译： 本文提供了一种微孔板，其具有在其中形成有至少一个阱的板体，阱具有第一开口端，第二端，在第二端形成的孔，以及在第一端和第二端之间延伸的侧壁 结束。 微板还具有至少部分地穿过形成在第二端中的孔的可渗透膜。 微板提供有渗透膜，其不仅允许从溶液（高分子量或低分子量溶质）中去除某些溶质，而且允许大分子混合物的分离和膜的降解。 微孔板还具有集成顶部组件或集成插入件。 本文还提供了在所述微量培养基中培养系统的方法。

公开(公告)号：US20100098742A1

公开(公告)日：2010-04-22

申请号：US10187247

申请日：2002-06-28

申请人： Joseph P. Vacanti ,  Young-Moon Michael Shin ,  Jennifer Ogilvie ,  Alexander Sevy ,  Tomoyuki Maemura ,  Osamu Ishii ,  Mohammad Reza Kaazempur-Mofrad ,  Jeffrey T. Borenstein ,  Kevin R. King ,  Chiao-Chun Wang ,  Eli Weinberg

发明人： Joseph P. Vacanti ,  Young-Moon Michael Shin ,  Jennifer Ogilvie ,  Alexander Sevy ,  Tomoyuki Maemura ,  Osamu Ishii ,  Mohammad Reza Kaazempur-Mofrad ,  Jeffrey T. Borenstein ,  Kevin R. King ,  Chiao-Chun Wang ,  Eli Weinberg

IPC分类号： A61K35/00 ,  A61K9/00 ,  A61K35/32 ,  A61K35/34

CPC分类号： A61F2/062 ,  A61F2240/004 ,  A61F2240/005 ,  A61L27/3604 ,  A61L27/3804 ,  A61L27/3808 ,  A61L27/3891 ,  A61L27/3895 ,  A61L27/58 ,  A61L2300/64 ,  B33Y80/00 ,  C12M21/08 ,  C12M23/06 ,  C12M23/30 ,  C12M25/02 ,  C12N5/0062 ,  C12N5/0697 ,  C12N2533/12 ,  C12N2533/30 ,  Y10T29/49826

摘要： Methods and materials for making complex, living, vascularized tissues for organ and tissue replacement, especially complex and/or thick structures, such as liver tissue is provided. Tissue lamina is made in a system comprising an apparatus having (a) a first mold or polymer scaffold, a semi-permeable membrane, and a second mold or polymer scaffold, wherein the semi-permeable membrane is disposed between the first and second molds or polymer scaffolds, wherein the first and second molds or polymer scaffolds have means defining microchannels positioned toward the semi-permeable membrane, wherein the first and second molds or polymer scaffolds are fastened together; and (b) animal cells. Methods for producing complex, three-dimensional tissues or organs from tissue lamina are also provided.

摘要翻译： 提供了用于制造器官和组织置换的复杂的，活的，血管化的组织，特别是复杂和/或较厚的结构例如肝组织的方法和材料。 在包括具有（a）第一模具​​或聚合物支架，半透膜和第二模具或聚合物支架的装置的系统中制造组织层，其中半透膜设置在第一和第二模具之间或 聚合物支架，其中第一和第二模具或聚合物支架具有限定朝向半透膜的微通道的装置，其中第一和第二模具或聚合物支架紧固在一起; 和（b）动物细胞。 还提供了从组织层片制备复合的三维组织或器官的方法。

公开(公告)号：US20170296322A1

公开(公告)日：2017-10-19

申请号：US15354865

申请日：2016-11-17

申请人： The Charles Stark Draper Laboratory, Inc. ,  The General Hospital Corporation

发明人： Joseph P. Vacanti ,  Young-Moon M. Shin ,  Jennifer Ogilvie ,  Alexander Sevy ,  Tomoyuki Maemura ,  Osamu Ishii ,  Mohammad R. Kaazempur-Mofrad ,  Jeffrey T. Borenstein ,  Kevin R. King ,  Chiao-Chun Wang ,  Eli Weinberg

IPC分类号： A61F2/06 ,  A61L27/38 ,  A61L27/58

CPC分类号： A61F2/062 ,  A61F2240/004 ,  A61F2240/005 ,  A61L27/3604 ,  A61L27/3804 ,  A61L27/3808 ,  A61L27/3891 ,  A61L27/3895 ,  A61L27/58 ,  A61L2300/64 ,  B33Y80/00 ,  C12M21/08 ,  C12M23/06 ,  C12M23/30 ,  C12M25/02 ,  C12M35/08 ,  C12N5/0062 ,  C12N5/0697 ,  C12N2533/12 ,  C12N2533/30 ,  Y10T29/49826

摘要： Methods and materials for making complex, living, vascularized tissues for organ and tissue replacement, especially complex and/or thick, structures, such as liver tissue is provided. Tissue lamina is made in a system comprising an apparatus having (a) a first mold or polymer scaffold, a semi-permeable membrane, and a second mold or polymer scaffold, wherein the semi-permeable membrane is disposed between the first and second molds or polymer scaffolds, wherein the first and second molds or polymer scaffolds have means defining microchannels positioned toward the semi-permeable membrane, wherein the first and second molds or polymer scaffolds are fastened together; and (b) animal cells. Methods for producing complex, three-dimensional tissues or organs from tissue lamina are also provided.

公开(公告)号：US09738860B2

公开(公告)日：2017-08-22

申请号：US14153591

申请日：2014-01-13

申请人： The Charles Stark Draper Laboratory, Inc. ,  The General Hospital Corporation

发明人： Joseph P. Vacanti ,  Young-Moon M. Shin ,  Jennifer Ogilvie ,  Alexander Sevy ,  Tomoyuki Maemura ,  Osamu Ishii ,  Mohammad R. Kaazempur-Mofrad ,  Jeffrey T. Borenstein ,  Kevin R. King ,  Chiao-Chun Wang ,  Eli Weinberg

IPC分类号： C12M3/00 ,  A61L27/36 ,  A61L27/38 ,  C12N5/00 ,  C12N5/071 ,  C12M1/12 ,  C12M1/00

CPC分类号： A61F2/062 ,  A61F2240/004 ,  A61F2240/005 ,  A61L27/3604 ,  A61L27/3804 ,  A61L27/3808 ,  A61L27/3891 ,  A61L27/3895 ,  A61L27/58 ,  A61L2300/64 ,  B33Y80/00 ,  C12M21/08 ,  C12M23/06 ,  C12M23/30 ,  C12M25/02 ,  C12N5/0062 ,  C12N5/0697 ,  C12N2533/12 ,  C12N2533/30 ,  Y10T29/49826

摘要： Methods and materials for making complex, living, vascularized tissues for organ and tissue replacement, especially complex and/or thick, structures, such as liver tissue is provided. Tissue lamina is made in a system comprising an apparatus having (a) a first mold or polymer scaffold, a semi-permeable membrane, and a second mold or polymer scaffold, wherein the semi-permeable membrane is disposed between the first and second molds or polymer scaffolds, wherein the first and second molds or polymer scaffolds have means defining microchannels positioned toward the semi-permeable membrane, wherein the first and second molds or polymer scaffolds are fastened together; and (b) animal cells. Methods for producing complex, three-dimensional tissues or organs from tissue lamina are also provided.

公开(公告)号：US20160177030A1

公开(公告)日：2016-06-23

申请号：US14892267

申请日：2014-05-13

申请人： NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY

发明人： Shinji SUGIURA ,  Toshiyuki TAKAGI ,  Fumiki YANAGAWA ,  Kimio SUMARU ,  Toshiyuki KANAMORI

IPC分类号： C08G65/333 ,  C12N5/00 ,  C12M1/00 ,  C08G81/00

CPC分类号： C08G65/33389 ,  C08G65/333 ,  C08G65/33337 ,  C08G69/40 ,  C08G81/00 ,  C12M23/20 ,  C12M23/30 ,  C12N5/0068 ,  C12N2537/10

摘要： The present invention provides a photodegradable cross-linking agent capable of manufacturing a photodegradable gel, which has appropriate moisture content and water solubility as a cell carrier and has strength that makes it possible to construct a complicated three-dimensional microstructure. The photodegradable cross-linking agent of the present invention includes a main chain 2 which is composed of branched polyethylene glycol having three or more branched chains and a photodegradable benzyl group 3 which is disposed on the terminus of the branched chains, in which the benzyl group has an active ester group 4, which is reactive with an amino group or a hydroxyl group, and one or more nitro groups in a benzene ring.

摘要翻译： 本发明提供一种能够制造光可降解凝胶的光降解性交联剂，其具有作为电池载体的适当的水分含量和水溶性，并具有能够构成复杂的三维微结构的强度。 本发明的光降解性交联剂包括主链2，其由具有三个或更多个支链的支链聚乙二醇和位于支链末端的可光降解的苄基3组成，其中苄基 具有与氨基或羟基反应的活性酯基团4和苯环中的一个或多个硝基。

公开(公告)号：US20170319746A1

公开(公告)日：2017-11-09

申请号：US15535322

申请日：2014-12-12

申请人： ECOLE POLYTECHNIQUE FEDERALE DE LAUSANNE (EPFL)

发明人： Matthias Lutolf ,  Andrea Negro

IPC分类号： A61L27/38 ,  C12P19/04 ,  B33Y70/00 ,  B33Y80/00 ,  B29C64/40 ,  A61L27/26 ,  A61L27/48 ,  A61L27/52 ,  A61L27/54 ,  A61L27/58 ,  C08J3/075 ,  C09D105/04 ,  C09D171/02 ,  C12N9/10 ,  C12N5/077 ,  C12N5/00 ,  C12M1/00 ,  C12M3/06 ,  C12M3/00 ,  B33Y10/00 ,  B29K71/00 ,  B29C64/112 ,  B29K105/00

CPC分类号： A61L27/3804 ,  A61L27/18 ,  A61L27/20 ,  A61L27/26 ,  A61L27/38 ,  A61L27/48 ,  A61L27/52 ,  A61L27/54 ,  A61L27/58 ,  A61L27/60 ,  A61L2300/414 ,  A61L2400/18 ,  A61L2430/00 ,  B29C64/112 ,  B29C64/40 ,  B29K2071/02 ,  B29K2105/0061 ,  B29K2995/0056 ,  B32B2556/00 ,  B33Y10/00 ,  B33Y70/00 ,  B33Y80/00 ,  C08J3/075 ,  C08J2305/04 ,  C08J2371/02 ,  C09D11/102 ,  C09D11/14 ,  C09D105/04 ,  C09D171/02 ,  C12M21/08 ,  C12M23/16 ,  C12M23/30 ,  C12M25/14 ,  C12M29/10 ,  C12M33/00 ,  C12N5/0062 ,  C12N5/0656 ,  C12N9/1044 ,  C12N2513/00 ,  C12N2533/74 ,  C12N2537/10 ,  C12P19/04 ,  C12Y203/02013 ,  C12Y402/02003 ,  C08L71/02 ,  C08L5/04

摘要： A composition comprising a first material and a second material, wherein said first material is cross-linkable by a first cross-linking reaction and said second material is cross-linkable by a second cross-linking reaction, wherein said first cross-linking reaction and said second cross-linking reaction are inducible by a common activator.