公开(公告)号：US11766469B2

公开(公告)日：2023-09-26

申请号：US17086786

申请日：2020-11-02

申请人： Quthero, Inc.

发明人： Serena Mandla ,  Milica Radisic

IPC分类号： A61K38/08 ,  A61K38/16 ,  A61K38/00 ,  A61P17/02 ,  A61F13/00 ,  A61K9/70 ,  A61K9/14 ,  A61K38/18 ,  C12N5/077 ,  A61K47/61 ,  A61K9/06 ,  G01N33/68 ,  A61K47/42

CPC分类号： A61K38/1891 ,  A61K9/06 ,  A61K9/14 ,  A61K9/70 ,  A61K9/7007 ,  A61K9/7015 ,  A61K38/08 ,  A61K47/42 ,  A61K47/61 ,  A61P17/02 ,  C12N5/0669 ,  G01N33/6893 ,  C12N2501/17

摘要： The present invention examines the interaction between an angiopoietin-1 mimetic peptide, QHREDGS (glutamine-histidine-arginine-glutamic acid-aspartic acid-glycine-serine (SEQ ID NO: 1)) immobilized to a collagen-chitosan hydrogel, and murine bone marrow derived macrophages. When macrophages were cultured in the presence of the peptide conjugated to a hydrogel, both pro-inflammatory and anti-inflammatory cytokines were produced, in contrast to the application of soluble peptide which elicited minimal cytokine secretion. This indicates a unique macrophage polarization with covalently immobilized peptide hydrogels, which can be beneficial in the context of the wound microenvironment.

公开(公告)号：US11666604B2

公开(公告)日：2023-06-06

申请号：US15779073

申请日：2017-12-18

申请人： INJE UNIVERSITY INDUSTRY-ACADEMIC COOPERATION FOUNDATION ,  AJOU UNIVERSITY INDUSTRY-ACADEMIC COOPERATION FOUNDATION

发明人： Young-Il Yang ,  Ki-Dong Park ,  Won-Jin Lee ,  Min-Young Choi ,  Kyung-Min Park ,  Yun-Ki Lee

IPC分类号： A61K35/34 ,  C12N5/0775

CPC分类号： A61K35/34 ,  C12N5/0662 ,  C12N2501/15 ,  C12N2501/155 ,  C12N2501/17 ,  C12N2501/235 ,  C12N2533/52 ,  C12N2533/54 ,  C12N2533/56

摘要： Disclosed are a multilayered cell sheet of cardiac stem cells (CSCs) and a method of manufacturing the same. In particular, the present disclosure provides a method of manufacturing a multilayered cell sheet according to a single step culture procedure by using, as a three-dimensional matrix, a biodegradable natural polymer hydrogel and embedding CSCs in the hydrogel. The multilayered cell sheet of the present disclosure does not require any special device for the manufacturing, is manageable with good physicomechanical property, increases a cell engraftment rate after transplantation based on sufficient accumulation of various growth and protective factors and extracellular matrix between cells, and is also self-assembled by the cell-mediated hydrogel compaction, making nutrients transfer easy. Therefore, the multilayered cell sheet of the CSCs is expected to be usefully applicable as a therapeutic agent for myocardium regeneration.

公开(公告)号：US09649359B2

公开(公告)日：2017-05-16

申请号：US14772209

申请日：2014-03-07

申请人： Kristen Johnson ,  Jian Shi

发明人： Kristen Johnson ,  Jian Shi

IPC分类号： A61K38/18 ,  A61K38/17 ,  C07K14/515 ,  C07K14/475 ,  A61K45/06 ,  A61K38/01 ,  A61K38/22 ,  A61K35/32 ,  A61K31/192 ,  A61K31/198 ,  A61K31/593 ,  A61K31/728 ,  C12N5/077

CPC分类号： A61K38/1891 ,  A61K31/192 ,  A61K31/198 ,  A61K31/593 ,  A61K31/728 ,  A61K35/32 ,  A61K38/014 ,  A61K38/1825 ,  A61K38/1875 ,  A61K38/225 ,  A61K45/06 ,  C07K14/515 ,  C12N5/0655 ,  C12N2501/17 ,  C12N2506/1346 ,  A61K2300/00

摘要： The present invention provides new protease resistant polypeptides, as well as compositions and methods for treating, ameliorating or preventing conditions related to joint damage, including acute joint injury and arthritis.

公开(公告)号：US08506955B2

公开(公告)日：2013-08-13

申请号：US12853677

申请日：2010-08-10

申请人： Chengcheng Zhang ,  Harvey F. Lodish

发明人： Chengcheng Zhang ,  Harvey F. Lodish

IPC分类号： C12N5/00

CPC分类号： C12N5/00 ,  A61K2035/124 ,  C12N5/0647 ,  C12N2500/90 ,  C12N2501/105 ,  C12N2501/113 ,  C12N2501/125 ,  C12N2501/145 ,  C12N2501/17 ,  C12N2501/91

摘要： Methods and kits for propagating hematopoietic stem cells are provided. The methods comprise culturing cells in medium comprising one or more angiopoietin-like proteins, under conditions sufficient for expansion of HSCs. Angiopoietin-like proteins include angiopoietin-like protein 2, angiopoietin-like protein 3, angiopoietin-like protein 4, angiopoietin-like protein 5, angiopoietin-like protein 7, and microfibrillar-associated glycoprotein (Mfap4). Methods for identifying hematopoietic stem cells are provided and isolated hematopoietic stem cells are also provided.

摘要翻译： 提供了用于繁殖造血干细胞的方法和试剂盒。 所述方法包括在足以扩增HSC的条件下，在包含一种或多种血管生成素样蛋白的培养基中培养细胞。 血管生成素样蛋白包括血管生成素样蛋白2，血管生成素样蛋白3，血管生成素样蛋白4，血管生成素样蛋白5，血管生成素样蛋白7和微原纤维相关糖蛋白（Mfap4）。 提供鉴定造血干细胞的方法，并提供分离的造血干细胞。

公开(公告)号：US20090117087A1

公开(公告)日：2009-05-07

申请号：US12250820

申请日：2008-10-14

申请人： David Carroll ,  William Wagner ,  Nicole Levi ,  Louis Argenta ,  Michael Morykwas

发明人： David Carroll ,  William Wagner ,  Nicole Levi ,  Louis Argenta ,  Michael Morykwas

IPC分类号： A61K35/12

CPC分类号： A61L27/38 ,  C12N5/0629 ,  C12N5/0656 ,  C12N2501/115 ,  C12N2501/165 ,  C12N2501/17 ,  C12N2533/54 ,  C12N2533/74 ,  C12N2535/10

摘要： A method of carrying out an autologous tissue implant in a subject in need thereof is carried out by: (a) forming an autologous tissue implant from autologous cells collected from a subject (e.g., by ink-jet printing, the autologous cells and the scaffold, separately or together), and then (b) implanting the autologous tissue implant in said subject.

摘要翻译： 在有需要的受试者中进行自体组织植入的方法通过以下方式进行：（a）从从受试者收集的自体细胞（例如通过喷墨印刷，自体细胞和支架）形成自体组织植入物 ，单独地或一起），然后（b）将自体组织植入物植入所述受试者。

公开(公告)号：US11773370B2

公开(公告)日：2023-10-03

申请号：US16627671

申请日：2018-07-02

申请人： CHA BIOTECH CO., LTD.

发明人： Ji Min Yu ,  Ae Ri Kim

IPC分类号： C12N5/071 ,  C12N5/00 ,  C12P21/02

CPC分类号： C12N5/0625 ,  C12P21/02 ,  C12N2500/38 ,  C12N2500/90 ,  C12N2501/17 ,  C12N2501/20 ,  C12N2501/39 ,  C12N2501/998 ,  C12N2501/999 ,  C12N2506/02 ,  C12N2506/025 ,  C12N2506/03

摘要： Provided are a method of preparing a stem cell-derived epidermal progenitor cell conditioned medium, the method including: differentiating stem cells to stem cell-derived epidermal progenitor cells by culturing the stem cells in a differentiation medium containing ascorbic acid and hydrocortisone; producing a culture of stem cell-derived epidermal progenitor cells by culturing the differentiated stem cell-derived epidermal progenitor cells in a medium; and recovering the stem cell-derived epidermal progenitor cell conditioned medium from the culture of the stem cell-derived epidermal progenitor cells, a stem cell-derived epidermal progenitor cell conditioned medium prepared by the method, and a method of producing a protein from stem cell-derived epidermal progenitor cells, the method including the method of preparing the stem cell-derived epidermal progenitor cell conditioned medium.

公开(公告)号：US11666684B2

公开(公告)日：2023-06-06

申请号：US15773171

申请日：2017-12-18

申请人： INJE UNIVERSITY INDUSTRY-ACADEMIC COOPERATION FOUNDATION

发明人： Young-Il Yang ,  Won-Jin Lee ,  Jong-Tae Kim

IPC分类号： A61L27/52 ,  A61L27/38 ,  C12N5/00 ,  C12N5/0797 ,  A61L27/26 ,  A61K35/30 ,  A61L27/36 ,  A61L27/22 ,  A61L27/24 ,  A61L27/54 ,  C12N5/0735 ,  A61P29/00

CPC分类号： A61L27/52 ,  A61K35/30 ,  A61L27/225 ,  A61L27/24 ,  A61L27/26 ,  A61L27/3604 ,  A61L27/3633 ,  A61L27/3658 ,  A61L27/3834 ,  A61L27/54 ,  C12N5/0068 ,  C12N5/0606 ,  C12N5/0623 ,  A61P29/00 ,  C12N2501/117 ,  C12N2501/119 ,  C12N2501/13 ,  C12N2501/135 ,  C12N2501/15 ,  C12N2501/165 ,  C12N2501/17 ,  C12N2501/231 ,  C12N2501/2306 ,  C12N2533/52 ,  C12N2533/54 ,  C12N2533/56 ,  C12N2533/90 ,  A61L27/26 ,  C08L89/06

摘要： A method of manufacturing a multilayered cell sheet of neural crest stem cells (NCSCs), includes: (1) isolating and culturing NCSCs from peripheral nerves; (2) embedding the cultured NCSCs in a hydrogel; (3) culturing the hydrogel comprising the NCSCs embedded therein under stressed culture conditions in which a physical support is applied; and (4) culturing the resulting hydrogel of step (3) under non-stressed culture conditions in which a physical support is removed.

公开(公告)号：US20170260508A1

公开(公告)日：2017-09-14

申请号：US15313043

申请日：2015-05-21

申请人： FRED HUTCHINSON CANCER RESEARCH CENTER ,  BOARD OF REGENTS OF THE UNIVERSITY OF TEXAS SYSTEM

发明人： Irwin D. Bernstein ,  Chengcheng Zhang ,  Mi Deng ,  Zhigang Lu ,  Junke Zheng

IPC分类号： C12N5/0789 ,  G01N33/569

CPC分类号： C12N5/0647 ,  C07K16/28 ,  C07K16/2863 ,  C07K16/2896 ,  C12N5/0662 ,  C12N2500/90 ,  C12N2501/113 ,  C12N2501/125 ,  C12N2501/145 ,  C12N2501/17 ,  C12N2501/2303 ,  C12N2501/2306 ,  C12N2501/26 ,  C12N2501/42 ,  C12N2501/599 ,  C12N2501/91 ,  G01N33/56966

摘要： The current disclosure describes methods of expanding precursor cells for hematopoietic transplantation in subjects. The methods culture precursor cells in media containing an immobilized high molecular weight LILRB2 agonist or an LILRB2 agonist in combination with a Notch agonist. The expanded cells can be used to treat a variety of hematopoietic disorders.

公开(公告)号：US09481867B2

公开(公告)日：2016-11-01

申请号：US14343390

申请日：2013-03-04

申请人： BEIJING GINKGO BIOSCIENCE CO., LTD.

发明人： Qinwei Yin

IPC分类号： C12N5/00 ,  C12N15/63 ,  C12N5/0789 ,  C12N15/11

CPC分类号： C12N5/0647 ,  C12N15/111 ,  C12N2310/141 ,  C12N2320/30 ,  C12N2501/105 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/125 ,  C12N2501/135 ,  C12N2501/145 ,  C12N2501/15 ,  C12N2501/17 ,  C12N2501/235 ,  C12N2501/26 ,  C12N2501/40 ,  C12N2501/41 ,  C12N2501/42 ,  C12N2501/65 ,  C12N2506/1384

摘要： A method of rapidly inducing large-scale and high-purity mesenchymal stem cells to transdetermine into hematopoietic stem cells is provided with the steps of preparing homogeneous medium of mesenchymal stem cells; combining a plurality of small RNA molecules; assembling and transfecting nanoparticles of nucleic acids and polypeptides; inducing and amplifying medium of post-transdetermined hematopoietic stem cells; and activating a plurality of hemopoiesis-related genes.

公开(公告)号：US08900868B2

公开(公告)日：2014-12-02

申请号：US13508821

申请日：2010-11-10

申请人： Donny Hanjaya-Putra ,  Sharon Gerecht

发明人： Donny Hanjaya-Putra ,  Sharon Gerecht

IPC分类号： A61K35/12 ,  A61K38/00 ,  C12N5/00 ,  A61L27/54 ,  C12N5/071 ,  A61L27/52 ,  A61L27/38

CPC分类号： C12N5/0692 ,  A61K35/44 ,  A61K2035/126 ,  A61L27/3808 ,  A61L27/52 ,  A61L27/54 ,  A61L2300/414 ,  C12N5/069 ,  C12N2500/34 ,  C12N2501/115 ,  C12N2501/148 ,  C12N2501/165 ,  C12N2501/17 ,  C12N2501/21 ,  C12N2501/998 ,  C12N2513/00 ,  C12N2533/50 ,  C12N2533/80 ,  C12N2537/10

摘要： A medium for growing vascular lineage cells is described. The vascular lineage cell growth medium includes an oligosaccharide-based hydrogel and a growth factor that promotes vascularization by vascular lineage cells.

摘要翻译： 描述了用于生长血管谱系细胞的培养基。 血管谱系细胞生长培养基包括寡糖基水凝胶和促进血管谱系细胞血管形成的生长因子。