公开(公告)号：US11920165B2

公开(公告)日：2024-03-05

申请号：US17811479

申请日：2022-07-08

申请人： Baylor College of Medicine

发明人： Mary K. Estes ,  David Graham ,  Robert Legare Atmar ,  Sue Ellen Crawford ,  Khalil Ettayebi ,  Kosuke Murakami

IPC分类号： C07K14/005 ,  A61K31/575 ,  A61K39/12 ,  A61K39/125 ,  C07K14/08 ,  C07K14/085 ,  C12N5/00 ,  C12N5/071 ,  C12N7/00 ,  C12N7/02 ,  G01N33/569

CPC分类号： C12N7/02 ,  A61K31/575 ,  C12N5/0018 ,  C12N5/0679 ,  C12N7/00 ,  G01N33/569 ,  C12N2770/16051

摘要： Embodiments of the disclosure concern systems, methods, and/or compositions for cultivation of mammalian viruses, including at least human noroviruses and sapoviruses within the Caliciviridae family of viruses. The ex vivo culture systems include intestinal enteroids in combination with bile or a functionally active fraction or component thereof. In specific embodiments, the culture system is utilized to test inactivation compounds for therapeutic or environmental efficacy and to test contaminated comestibles and/or environmental entities for determination of the presence of infectious virus. Furthermore, antiviral compositions may be tested using systems of the disclosure, including drugs, small molecule inhibitors, and biologics such as neutralizing monoclonal antibodies.

公开(公告)号：US11826418B1

公开(公告)日：2023-11-28

申请号：US18151409

申请日：2023-01-06

申请人： KING FAISAL UNIVERSITY

发明人： Jawhar Gharbi ,  Ikbel Hadj Hassine ,  Mohammed A. Almalki ,  Ameera Alyami ,  Manel Ben M'Hadheb

IPC分类号： A61K39/00 ,  A61K39/12 ,  A61K39/125 ,  C07K14/005 ,  C07K14/085 ,  A61K39/135 ,  A61K9/00 ,  A61P31/14

CPC分类号： A61K39/135 ,  A61K9/0019 ,  A61P31/14 ,  A61K2039/5258 ,  A61K2039/55555 ,  A61K2039/6081

摘要： The virus-like particle (VLP)-based vaccine against CVB4 infection includes a virus-like particle (VLP) derived from VP1 of Coxsackievirus B4 (CVB4). The vaccine is devoid of virus RNA. The virus-like particles may be in nanoparticle form and coated with a polymer coating. The polymeric coating may be albumin, e.g., bovine serum albumin (BSA).

公开(公告)号：US11613764B2

公开(公告)日：2023-03-28

申请号：US16512031

申请日：2019-07-15

申请人： MINK Therapeutics, Inc.

发明人： Marc van Dijk ,  Volker Seibert ,  Cornelia Anne Mundt ,  Arthur Andrew Hurwitz ,  Mark Adrian Exley ,  Benjamin Jacob Wolf ,  Daniel Leventhal ,  Sébastien Lalevée ,  Reed Masakayan

IPC分类号： C12N15/85 ,  A61K47/64 ,  C07K14/725 ,  C07K16/18 ,  A61K47/65 ,  C07K14/085 ,  C07K14/74

摘要： Provided are TCRs (e.g., TCRs that bind to NY-ESO-1), cells and pharmaceutical compositions comprising these TCRs, nucleic acids encoding these TCRs, expression vectors and host cells for making these TCRs, and methods of treating a subject using these TCRs.

公开(公告)号：US20210128714A1

公开(公告)日：2021-05-06

申请号：US17259789

申请日：2018-11-30

申请人： MEDICAGO INC.

发明人： Marc-Andre D'AOUST ,  Pierre-Olivier LAVOIE

IPC分类号： A61K39/12 ,  C12N15/82 ,  C07K14/085

摘要： Nucleic acids encoding modified norovirus VP1 proteins, and VLPs comprising one or more of the modified norovirus VP1 proteins are provided. Methods for modified norovirus VP1 protein, and norovirus VLP, production in plants, portions of the plant or a plant cell, are also described.

公开(公告)号：US09402892B2

公开(公告)日：2016-08-02

申请号：US13977399

申请日：2011-12-29

申请人： Andrew MacAdam

发明人： Andrew MacAdam

IPC分类号： C07K14/105 ,  C07K14/085 ,  A61K39/13 ,  A61K39/125 ,  C12N15/43 ,  C12N15/41 ,  C12N7/00 ,  A61K39/12 ,  A61K39/00

CPC分类号： A61K39/13 ,  A61K39/12 ,  A61K39/125 ,  A61K2039/5252 ,  A61K2039/5254 ,  A61K2039/5256 ,  C12N7/00 ,  C12N2770/32011 ,  C12N2770/32611 ,  C12N2770/32621 ,  C12N2770/32634 ,  C12N2770/32662 ,  G01N2333/085 ,  Y02A50/466

摘要： The invention provides an attenuated polio virus having a 5′ non-coding region consisting of the 5′ non-coding region of Sabin 3, modified so that it does not have a base pair mismatch in stem (a) or (b) of domain V, wherein seven or eight of the base pairs in stems (a) and (b) are U-A or A-U base pairs; and a capsid protein from the Sabin 1, Mahoney, MEF or Saukett strain.

摘要翻译： 本发明提供了具有由Sabin 3的5'非编码区组成的5'非编码区的减毒脊髓灰质炎病毒，所述5'非编码区被修饰，使得其在结构域的茎（a）或（b）中不具有碱基对失配 V，其中茎（a）和（b）中的七个或八个碱基对是UA或AU碱基对; 和Sabin 1，Mahoney，MEF或Saukett菌株的衣壳蛋白。

公开(公告)号：US20120027777A1

公开(公告)日：2012-02-02

申请号：US13145871

申请日：2010-01-22

申请人： Michael Lindberg ,  Conny Tolf

发明人： Michael Lindberg ,  Conny Tolf

IPC分类号： A61K39/42 ,  C07H21/00 ,  C07K14/085 ,  A61K39/125 ,  C07K16/10 ,  C12N7/04 ,  C07D413/12 ,  A61K31/4245 ,  A61P9/00 ,  A61P3/10 ,  A61P25/00 ,  A61P43/00 ,  A61P37/04 ,  A61P31/14 ,  A61P15/00 ,  C12N7/00

CPC分类号： C07K14/005 ,  A61K39/00 ,  A61K39/12 ,  A61K39/125 ,  A61K2039/505 ,  A61K2039/5258 ,  A61K2039/53 ,  C12N2770/32522 ,  C12N2770/32534

摘要： The present invention relates to a Ljungan virus with improved replication characteristic and the use of this Ljungan virus, amongst other thing, in the production of a vaccine.

摘要翻译： 本发明涉及具有改进的复制特征的Ljungan病毒以及这种Ljungan病毒在生产疫苗中的用途。

公开(公告)号：US20050019808A1

公开(公告)日：2005-01-27

申请号：US10888143

申请日：2004-07-09

申请人： Ann Palmenberg ,  Aleksey Aminev ,  Rachel Groppo

发明人： Ann Palmenberg ,  Aleksey Aminev ,  Rachel Groppo

IPC分类号： C07K14/085 ,  C12N15/67 ,  C12Q1/68 ,  C07H21/04 ,  C12N9/22

CPC分类号： C07K14/005 ,  A61K2039/5256 ,  C12N15/67 ,  C12N2770/32222 ,  C12N2770/32243

摘要： The invention relates to compositions, specifically novel nucleic acid constructs encoding a cardiovirus 2A polypeptide operably linked to suitable promoters. Also, disclosed are methods whereby the nucleic acid constructs are introduced into cells or cell free systems to regulate cellular mRNA transcription and cap-dependent or internal ribosomal entry site (IRES)-dependent mRNA translation.

摘要翻译： 本发明涉及组合物，特别是编码与合适的启动子可操作地连接的心脏病毒2A多肽的新型核酸构建体。 还公开了将核酸构建体引入细胞或无细胞系统以调节细胞mRNA转录和帽依赖性或内部核糖体进入位点（IRES） - 依赖性mRNA翻译的方法。

公开(公告)号：US6057096A

公开(公告)日：2000-05-02

申请号：US479389

申请日：1995-06-07

申请人： Kenneth J. Rothschild ,  Sanjay M. Sonar ,  Jerzy Olejnik

发明人： Kenneth J. Rothschild ,  Sanjay M. Sonar ,  Jerzy Olejnik

IPC分类号： G01N33/531 ,  A61K9/127 ,  A61K38/00 ,  A61K38/17 ,  A61K39/00 ,  A61K41/00 ,  A61K47/48 ,  A61K49/00 ,  A61K51/00 ,  A61P31/04 ,  A61P31/12 ,  C07C205/45 ,  C07C205/57 ,  C07D207/40 ,  C07D207/404 ,  C07D207/46 ,  C07D311/16 ,  C07D405/12 ,  C07D495/04 ,  C07H19/04 ,  C07H19/06 ,  C07H19/16 ,  C07H21/00 ,  C07H21/04 ,  C07K1/107 ,  C07K1/13 ,  C07K14/00 ,  C07K14/02 ,  C07K14/03 ,  C07K14/085 ,  C07K14/155 ,  C07K14/28 ,  C07K14/465 ,  C07K19/00 ,  C12N15/09 ,  C12P21/00 ,  C12Q1/00 ,  C12Q1/02 ,  C12Q1/04 ,  C12Q1/25 ,  C12Q1/68 ,  C12Q1/70 ,  G01N33/532 ,  C07H19/00 ,  C12Q19/34

CPC分类号： C12Q1/6876 ,  A61K41/0042 ,  C07C205/45 ,  C07C205/57 ,  C07D207/404 ,  C07D311/16 ,  C07D405/12 ,  C07D495/04 ,  C07H19/04 ,  C07H21/00 ,  C07K1/1077 ,  C07K1/13 ,  C12P21/00 ,  G01N33/532 ,  A61K38/00

摘要： This invention relates to agents and conjugates that can be used to detect and isolate target components from complex mixtures such as nucleic acids from biological samples, cells from bodily fluids, and nascent proteins from translation reactions. Agents comprise a detectable moiety bound to a photoreactive moiety. Conjugates comprise agents coupled to substrates by covalent bounds which can be selectively cleaved with the administration of electromagnetic radiation. Targets substances labeled with detectable molecules can be easily identified and separated from a heterologous mixture of substances. Exposure of the conjugate to radiation releases the target in a functional form and completely unaltered. Using photocleavable molecular precursors as the conjugates, label can be incorporated into macromolecules, the nascent macromolecules isolated and the label completely removed. The invention also relates to targets isolated with these conjugates which may be useful as pharmaceutical agents or compositions that can be administered to humans and other mammals. Useful compositions include biological agents such as nucleic acids, proteins, lipids and cytokines. Conjugates can also be used to monitor the pathway and half-life of pharmaceutical composition in vivo and for diagnostic, therapeutic and prophylactic purposes. The invention also relates to kits comprised of agents and conjugates that can be used for the detection of diseases, disorders and nearly any individual substance in a complex background of substances.

公开(公告)号：US5986076A

公开(公告)日：1999-11-16

申请号：US345807

申请日：1994-11-22

申请人： Kenneth J. Rothschild ,  Sanjay M. Sonar ,  Jerzy Olejnik

发明人： Kenneth J. Rothschild ,  Sanjay M. Sonar ,  Jerzy Olejnik

IPC分类号： G01N33/531 ,  A61K9/127 ,  A61K38/00 ,  A61K38/17 ,  A61K39/00 ,  A61K41/00 ,  A61K47/48 ,  A61K49/00 ,  A61K51/00 ,  A61P31/04 ,  A61P31/12 ,  C07C205/45 ,  C07C205/57 ,  C07D207/40 ,  C07D207/404 ,  C07D207/46 ,  C07D311/16 ,  C07D405/12 ,  C07D495/04 ,  C07H19/04 ,  C07H19/06 ,  C07H19/16 ,  C07H21/00 ,  C07H21/04 ,  C07K1/107 ,  C07K1/13 ,  C07K14/00 ,  C07K14/02 ,  C07K14/03 ,  C07K14/085 ,  C07K14/155 ,  C07K14/28 ,  C07K14/465 ,  C07K19/00 ,  C12N15/09 ,  C12P21/00 ,  C12Q1/00 ,  C12Q1/02 ,  C12Q1/04 ,  C12Q1/25 ,  C12Q1/68 ,  C12Q1/70 ,  G01N33/532 ,  C07H19/00 ,  A01N37/18 ,  A61K39/245

CPC分类号： C12Q1/6876 ,  A61K41/0042 ,  C07C205/45 ,  C07C205/57 ,  C07D207/404 ,  C07D311/16 ,  C07D405/12 ,  C07D495/04 ,  C07H19/04 ,  C07H21/00 ,  C07K1/1077 ,  C07K1/13 ,  C12P21/00 ,  G01N33/532 ,  A61K38/00

摘要： This invention relates to agents and conjugates that can be used to detect and isolate target components from complex mixtures such as nucleic acids from biological samples, cells from bodily fluids, and nascent proteins from translation reactions. Agents comprise a detectable moiety bound to a photoreactive moiety. Conjugates comprise agents coupled to substrates by covalent bounds which can be selectively cleaved with the administration of electromagnetic radiation. Targets substances labeled with detectable molecules can be easily identified and separated from a heterologous mixture of substances. Exposure of the conjugate to radiation releases the target in a functional form and completely unaltered. Using photocleavable molecular precursors as the conjugates, label can be incorporated into macromolecules, the nascent macromolecules isolated and the label completely removed. The invention also relates to targets isolated with these conjugates which may be useful as pharmaceutical agents or compositions that can be administered to humans and other mammals. Useful compositions include biological agents such as nucleic acids, proteins, lipids and cytokines. Conjugates can also be used to monitor the pathway and half-life of pharmaceutical composition in vivo and for diagnostic, therapeutic and prophylactic purposes. The invention also relates to kits comprised of agents and conjugates that can be used for the detection of diseases, disorders and nearly any individual substance in a complex background of substances.

公开(公告)号：US4857634A

公开(公告)日：1989-08-15

申请号：US89037

申请日：1987-08-24

申请人： Philip D. Minor ,  David M. A. Evans ,  Geoffrey C. Schild ,  Jeffrey W. Almond ,  Morag Ferguson

发明人： Philip D. Minor ,  David M. A. Evans ,  Geoffrey C. Schild ,  Jeffrey W. Almond ,  Morag Ferguson

IPC分类号： G01N33/569 ,  A61K39/00 ,  A61K39/13 ,  C07K14/00 ,  C07K14/085 ,  C07K14/105 ,  C07K17/00 ,  C12N15/09 ,  C12P21/00

CPC分类号： C07K14/005 ,  A61K39/00 ,  C12N2770/32322 ,  C12N2770/32622

摘要： A synthetic peptide, suitable for use in vaccination against or diagnosis of a disease caused by an enterovirus and especially by a poliovirus, is the peptide coded for by codons 286-290 in the RNA sequence coding for the structural capsid protein VP1 of poliovirus type 3 Sabin strain or by equivalent codons of another enterovirus or is an antigenic equivalent thereof, the numbers of the codons being counted from the 5'-terminus of the nucleotide sequence coding for the VP1 capsid protein.

摘要翻译： 适用于针对或诊断肠道病毒特别是脊髓灰质炎病毒引起的疾病的合成肽是编码脊髓灰质炎病毒3型结构衣壳蛋白VP1的RNA序列中的密码子286-290所编码的肽 Sabin菌株或其他肠道病毒的等价密码子或其抗原等价物，密码子的数目从编码VP1衣壳蛋白的核苷酸序列的5'端计数。