公开(公告)号：US20240336936A1

公开(公告)日：2024-10-10

申请号：US18680137

申请日：2024-05-31

申请人： LONZA WALKERSVILLE, INC.

发明人： Young SHIN ,  Anandita SETH ,  Bingnan GU

IPC分类号： C12N15/867 ,  C07K14/16 ,  C12N5/071 ,  C12N9/12 ,  C12N15/86

CPC分类号： C12N15/867 ,  C07K14/161 ,  C07K14/162 ,  C07K14/163 ,  C12N5/0686 ,  C12N9/1241 ,  C12N15/8673 ,  C12N15/8676 ,  C12N15/86 ,  C12N2740/15051 ,  C12N2740/16052 ,  C12N2760/20222

摘要： The present disclosure relates to methods for producing lentiviral vector-producing cells. Specifically the methods utilize two plasmids, rather than four, to provide the required packaging elements and transfer vector to a cell, allowing for the production of a large number of lentiviral producer cells, including suspension-based cells, and the production of high amounts of lentivirus. These methods allow for the production of cells that can be later induced to produce lentivirus, and can be tailored to include a specific gene of interest.

公开(公告)号：US12105091B2

公开(公告)日：2024-10-01

申请号：US16761786

申请日：2018-11-06

申请人： VIB VZW ,  Universiteit Gent

发明人： An Hendrix ,  Olivier De Wever ,  Edward Geeurickx ,  Joke Vandesompele ,  Pieter Mestdagh ,  Sven Eyckerman

IPC分类号： G01N33/58 ,  C07K14/16 ,  C07K14/435 ,  C12Q1/6876 ,  G01N21/64 ,  G01N33/53

CPC分类号： G01N33/582 ,  C07K14/161 ,  C07K14/43595 ,  C12Q1/6876 ,  G01N21/6428 ,  G01N33/5308 ,  C07K2319/60 ,  C12Q2600/158 ,  G01N2021/6439 ,  G01N2496/30

摘要： This disclosure relates to the field of research and clinical usage of extracellular vesicles. More in particular, this disclosure relates to usages of recombinant extracellular vesicles comprising a) a self-assembling protein that directs its own release through vesicles as a luminal membrane-bound protein and b) a heterologous marker. Indeed, the disclosure provides recombinant extracellular vesicles that can be used as a biological reference material in methods to quantify extracellular vesicles in a sample, and/or, that can be used to calibrate a device for sample extracellular vesicles analysis, and/or, that can be used to evaluate the isolation process of extracellular vesicles. This disclosure thus relates to improving the accuracy of an extracellular vesicle-based diagnosis.

公开(公告)号：US12053520B2

公开(公告)日：2024-08-06

申请号：US17587817

申请日：2022-01-28

申请人： The United States of America, as represented by the Secretary, Department of Health and Human Services

发明人： Xuejun Chen ,  Jeffrey Boyington ,  Hongying Duan Holdsworth ,  Cheng Cheng ,  John R. Mascola

IPC分类号： A61K39/21 ,  C07K14/005 ,  C07K14/16 ,  C12N7/00 ,  A61K39/00

CPC分类号： A61K39/21 ,  C07K14/005 ,  C07K14/162 ,  C12N7/00 ,  C12Y205/01078 ,  A61K2039/5258 ,  A61K2039/55555 ,  C07K2319/00 ,  C12N2740/16023 ,  C12N2740/16043 ,  C12N2740/16111 ,  C12N2740/16122 ,  C12N2740/16134 ,  C12N2740/16171

摘要： Embodiments of immunogens based on the outer domain of HIV-1 gp120 and methods of their use and production are disclosed. Nucleic acid molecules encoding the immunogens are also provided. In several embodiments, the immunogens can be used to prime an immune response to gp120 in a subject, for example, to treat or prevent an HIV-1 infection in the subject.

公开(公告)号：US20240199706A1

公开(公告)日：2024-06-20

申请号：US17999898

申请日：2021-05-28

申请人： COMMISSARIAT A L'ENERGIE ATOMIQUE ET AUX ENERGIES ALTERNATIVES

发明人： Michel LEONETTI ,  Alexandra SAVATIER ,  Celine COUSIN ,  Culina SLOBODAN

IPC分类号： C07K14/16 ,  A61K38/00 ,  A61K39/00 ,  A61P37/04 ,  C07K14/34 ,  C07K14/705 ,  C07K14/735 ,  C07K16/28

CPC分类号： C07K14/163 ,  A61P37/04 ,  C07K14/34 ,  C07K14/70535 ,  C07K14/7056 ,  C07K16/2803 ,  A61K38/00 ,  A61K2039/505

摘要： The invention relates to a molecular complex consisting of at least one ligand of a sulphated sugar of the glycosaminoglycan family linked to at least one ligand of a surface molecule of antigen-presenting cells or NK or NKT cells, for use as an immunomodulatory drug, in particular in the immunotherapy of cancer and infectious diseases.

公开(公告)号：US11767347B2

公开(公告)日：2023-09-26

申请号：US17553070

申请日：2021-12-16

申请人： The Scripps Research Institute

发明人： Leopold Kong ,  Ian A. Wilson ,  Natalia De Val ,  Andrew B. Ward ,  Dennis Burton ,  Linling He ,  Jiang Zhu

IPC分类号： C07K14/16 ,  A61K47/69 ,  A61K39/21 ,  C07K14/005 ,  A61K39/12 ,  A61K47/64 ,  A61K39/00

CPC分类号： C07K14/162 ,  A61K39/21 ,  A61K47/6929 ,  C07K14/005 ,  A61K39/12 ,  A61K47/64 ,  A61K2039/5258 ,  A61K2039/6031 ,  A61K2039/645 ,  C12N2740/16122 ,  C12N2740/16134

摘要： The present invention provides HIV-1 vaccine immunogens. Some of the immunogens contain a soluble gp140-derived protein that harbors a modified N-terminus of the HR1 region in gp41. Some of the immunogens contain an HIV-1 Env-derived trimer protein that is presented on a nanoparticle platform. The invention also provides methods of using the HIV-1 vaccine immunogens for eliciting an immune response or treating HIV infections.

公开(公告)号：US11692012B2

公开(公告)日：2023-07-04

申请号：US17214598

申请日：2021-03-26

申请人： Oregon Health & Science University

发明人： Klaus Frueh ,  Scott G. Hansen ,  Jay Nelson ,  Louis Picker ,  Patrizia Caposio

IPC分类号： C07K14/045 ,  A61K39/12 ,  C12N15/86 ,  A61K35/33 ,  A61K39/00 ,  A61K39/39 ,  C12N15/85 ,  A61K39/21 ,  C07K14/16 ,  A61P35/00

CPC分类号： C07K14/045 ,  A61K35/33 ,  A61K39/0011 ,  A61K39/12 ,  A61K39/39 ,  C12N15/85 ,  C12N15/86 ,  A61K39/21 ,  A61K2039/5254 ,  A61K2039/5256 ,  A61K2039/572 ,  A61P35/00 ,  C07K14/161 ,  C12N2710/16143 ,  C12N2740/15034 ,  C12N2740/16234

摘要： Human cytomegalovirus vectors comprising heterologous antigens are disclosed. The vectors derived from the TR strain, are ganciclovir sensitive, include active US2, US3, US6, US7 and UL131A genes, and have a deleterious or inactivating mutation in the UL82 gene preventing the expression of pp71.

公开(公告)号：US20230181717A1

公开(公告)日：2023-06-15

申请号：US17550768

申请日：2021-12-14

申请人： THE SCRIPPS RESEARCH INSTITUTE

发明人： Linling He ,  Jiang Zhu

IPC分类号： A61K39/21 ,  A61K47/69 ,  A61P31/18 ,  A61K9/51 ,  A61K38/16 ,  C07K14/16

CPC分类号： A61K39/21 ,  A61K47/6929 ,  A61P31/18 ,  A61K9/51 ,  A61K38/162 ,  C07K14/162 ,  A61K2039/55516

摘要： The present invention provides methods for producing HIV-1 nanoparticle vaccines with enhanced immunogenicity. The methods entail (1) enzymatic digestion of glycan chain on the surface of a self-assembling nanoparticle vaccine displaying an HIV-1 Env derived trimer immunogen, or (2) expression of an HIV-1 nanoparticle construct in an expression system lacking normal glycosylation function for human proteins. Also provided in the invention are HIV-1 nanoparticle vaccines produced with the described methods. The invention further provides methods of using the HIV-1 nanoparticle vaccine compositions described herein in various therapeutic applications, e.g., for preventing or treating viral infections.

公开(公告)号：US11564947B2

公开(公告)日：2023-01-31

申请号：US17025607

申请日：2020-09-18

申请人： eFFECTOR Therapeutics, Inc.

发明人： Kevin R. Webster ,  Rajesh Sharma ,  Gary Chiang

IPC分类号： C07H21/04 ,  A61K35/17 ,  C07K14/74 ,  A61P35/00 ,  C07K14/725 ,  C07K14/73 ,  C07K14/16 ,  C07K14/705 ,  C12N5/0783 ,  C07D471/04 ,  C07D487/04 ,  C07D495/20 ,  C07D491/20 ,  C07D471/20 ,  A61K31/501 ,  A61K45/06

摘要： The present disclosure relates to genetically modified T cells comprising a transgene encoding an engineered antigen specific receptor, wherein expression of an endogenous gene selected from MNK1, MNK2, or both are inhibited in the genetically modified T cell in order to enhance central memory T cell subsets in cellular immunotherapy compositions.

公开(公告)号：US20230014906A1

公开(公告)日：2023-01-19

申请号：US17816708

申请日：2022-08-01

申请人： ALBAJUNA THERAPEUTICS, S.L.

发明人： JORGE CARRILLO MOLINA ,  BONAVENTURA CLOTET SALA ,  JULIA M. BLANCO ARBUES

IPC分类号： C07K14/735 ,  C07K14/16 ,  C07K14/73 ,  C12N15/62 ,  C07K14/005 ,  C07K14/72

摘要： Fc-fusion protein derivatives against HIV have enhanced yield in mammalian cells, and extended antiviral and immunomodulatory activities. The Fc-fusion protein derivatives can block the entry of human immunodeficiency virus (HIV) into host cells, elicit effector functions through the activation of natural killer (NK) and other immune system cells, can be produced with high yield in mammalian cells, and have extended activity in vivo. Nucleic acids, vectors and host cells can express the Fc-fusion protein derivatives, which have therapeutic and diagnostic applications in human health.

公开(公告)号：US11535903B2

公开(公告)日：2022-12-27

申请号：US16966414

申请日：2019-01-31

申请人： Juno Therapeutics, Inc.

发明人： Ruth Berry ,  Edwin Webb

IPC分类号： C12Q1/70 ,  C12Q1/6851 ,  C12Q1/686 ,  C07K14/16

摘要： Provided are methods of detecting replication competent virus, e.g., replication competent retrovirus such as gammaretrovirus or lentivirus, in a sample containing a cell transduced with a viral vector particle encoding a recombinant and or heterologous molecule, e.g., heterologous gene product. The methods may include assessing transcription of one or more target genes, such as viral genes, that are expressed in a retrovirus but not expressed in the viral vector particle. Replication competent retrovirus may be determined to be present if the levels of RNA of the one or more target genes is higher than a reference value, which can be measured directly or indirectly, including from a positive control sample containing RNA from the respective target gene at a known level and/or at or above the limit of detection of the assay.