公开(公告)号：US10519474B2

公开(公告)日：2019-12-31

申请号：US15579291

申请日：2016-06-02

Applicant: BASF SE

Inventor： Qingzhao Wang ,  Shakir Siraj Ratani ,  Zheyuan Guo ,  Hartwig Schroeder ,  Holger Hartmann ,  Markus Pompejus

IPC: C12P13/06 ,  C07K14/245 ,  C12N1/20 ,  C12N15/70

Abstract: Recombinant microorganism for improved production of fine chemicals The present invention relates to a recombinant nucleic acid molecule, a recombinant micro-organism, to a method for producing alanine and to the use of the recombinant nucleic acid molecule or the recombinant microorganism for the fermentative production of alanine.

公开(公告)号：US10150777B2

公开(公告)日：2018-12-11

申请号：US14778788

申请日：2014-03-03

Applicant: BASF SE

Inventor： Burkhard Ernst ,  Manfred Ehresmann ,  Christopher Koradin ,  Andreas Pletsch ,  Melanie Bonnekessel ,  Thomas Kaeding ,  Karin Schein-Albrecht ,  Stefanie Demming ,  Franz Weber ,  Wolfgang Siegel ,  Hartwig Schroeder ,  Stephan Freyer ,  Peter Oedman

IPC: C07D493/04 ,  C12P17/18 ,  A01N43/40

Abstract: The invention pertains to processes to produce dry biomass of pyripyropene producer organisms, processes to obtain pyripyropenes from such dry biomass, as well as to processes to produce compounds of Formula III and/or Formula IV and/or Formula V from the pyripyropenes obtained from the dry biomass. The invention does further pertain to the dry biomass itself, as well as processes using said dry biomass to obtain pyripyropenes for the production of compounds of Formula III and/or Formula IV and/or Formula V, including processes using said dry biomass to obtain pyripyropenes or compounds of Formula III and/or Formula IV and/or Formula V in order to produce pest control compositions, in particular insecticides, comprising such compounds.

公开(公告)号：US09828617B2

公开(公告)日：2017-11-28

申请号：US14421872

申请日：2013-08-13

Applicant: BASF SE ,  Evolva SA

Inventor： Michael Naesby ,  Christophe Folly ,  Curt Aimé Friis Nielsen ,  Anaelle Hatsch ,  Markus Schwab ,  Oskar Zelder ,  Stefan Haefner ,  Hartwig Schroeder ,  Birgit Hoff ,  Andrea Molt ,  Klaus Ditrich ,  Michael Breuer ,  Holger Hartmann ,  Karsten Koerber

IPC: C12N15/53 ,  C12P17/12 ,  C12N9/02 ,  C12N9/90 ,  C12N15/52 ,  C12P17/10 ,  C12P17/18 ,  C07D209/90 ,  C07D487/06 ,  C07K16/40 ,  C12N9/10

CPC classification number: C12P17/12 ,  C07D209/90 ,  C07D487/06 ,  C07K16/40 ,  C12N9/0004 ,  C12N9/001 ,  C12N9/1007 ,  C12N9/1085 ,  C12N9/90 ,  C12N15/52 ,  C12P17/10 ,  C12P17/182 ,  C12Y205/01

Abstract: Microorganisms and processes for the recombinant manufacture of clavine-type alkaloids such as cycloclavine, festuclavine, agroclavine, chanoclavine and chanoclavine aldehyde, as well as polypeptides, polynucleotides and vectors comprising such polynucleotides which can be applied in a method for the manufacture of clavine-type alkaloids are provided.

公开(公告)号：US11279955B2

公开(公告)日：2022-03-22

申请号：US16614772

申请日：2018-05-18

Applicant: BASF SE

Inventor： Hartwig Schroeder ,  Stefan Haefner ,  Oskar Zelder ,  Christoph Wittmann ,  Anna Christine Schroer ,  Birgit Hoff ,  Nico Boehmer

IPC: C12N1/20 ,  C12P7/18 ,  C12P7/46

Abstract: Described herein is a process of producing an organic compound, the process including: I) cultivating a genetically modified microorganism in a culture medium including sucrose as an assimilable carbon source to allow the genetically modified microorganism to produce the organic compound, and II) recovering the organic compound from the fermentation broth obtained in process step I) The genetically modified microorganism includes A) at least one genetic modification that leads to an increased activity of the enzyme encoded by the rbsK-gene, compared to the original microorganism that has not been genetically modified, and the original microorganism belongs to the family Pasteurellaceae. Also described herein are a genetically modified microorganism and the use thereof for the fermentative production of an organic compound from sucrose as an assimilable carbon source.

公开(公告)号：US10287571B2

公开(公告)日：2019-05-14

申请号：US15308874

申请日：2015-05-07

Applicant: BASF SE

Inventor： Joanna Martyna Krawczyk ,  Stefan Haefner ,  Hartwig Schroeder ,  Oskar Zelder

IPC: A61K38/00 ,  C12N15/10 ,  C12P7/46 ,  C07K14/195 ,  C12N15/74

Abstract: The present invention relates to a modified microorganism having, compared to its wildtype, —a reduced activity of an enzyme encoded by the ptsA-gene, —a reduced activity of an enzyme encoded by the ptsH-gene or —a reduced activity of an enzyme encoded by the ptsA-gene and a reduced activity of an enzyme encoded by the ptsH-gene, wherein the wildtype from which the modified microorganism has been derived belongs to the family of Pasteurellaceae. The present invention also relates to a method for producing succinic acid and to the use of modified microorganisms.

公开(公告)号：US09631212B2

公开(公告)日：2017-04-25

申请号：US14441215

申请日：2013-11-13

Applicant: BASF SE

Inventor： Oskar Zelder ,  Birgit Hoff ,  Hartwig Schroeder ,  Andrea Molt ,  Holger Hartmann ,  Klaus Ditrich ,  Michael Breuer ,  Rüdiger Reingrubber ,  Jakob Weber

IPC: C12N15/80 ,  C12P17/04 ,  C12N15/67 ,  C07D307/93 ,  C12N15/52 ,  C07K14/37 ,  A01N37/46 ,  A01N63/04 ,  A01N37/42

CPC classification number: C12P17/04 ,  A01N37/42 ,  A01N37/46 ,  A01N63/04 ,  C07D307/93 ,  C07K14/37 ,  C12N15/52 ,  C12N15/67 ,  C12N15/80

Abstract: The invention pertains to the field of production of natural products and, in particular, in the field of production of cornexistin and hydroxycornexistin. It provides polynucleotides encoding polypeptides involved in the biosynthesis of cornexistin and hydroxycornexistin as well as vectors and recombinant microorganisms comprising such polynucleotides. Also provided are methods for the production of natural products, in particular methods for the production of cornexistin and hydroxycornexistin, using such polynucleotides and polpeptides encoded therein, as well as vectors and recombinant microorganisms comprising such polynucleotides and polypeptides.

公开(公告)号：US11685759B2

公开(公告)日：2023-06-27

申请号：US17286474

申请日：2019-10-17

Applicant: BASF SE

Inventor： Michael Puhl ,  Sebastian Wloch ,  Peter Oedman ,  Anne-Catrin Letzel ,  Hartwig Schroeder ,  Jacek Malisz ,  Daniel Seibert-Ludwig ,  Emiel Jan Kappert ,  Chung Huan Wong ,  Michael Barros Gross ,  Martin Viertelhaus

IPC: C07H1/08 ,  C07H3/06

CPC classification number: C07H1/08 ,  C07H3/06 ,  C07B2200/13

Abstract: The present invention relates to a method for obtaining crystalline 2′-fucosyllactose from a 2′-FL raw material, which contains 2′-FL as a main constituent and at least 0.5% by weight, frequently at least 1% by weight, in particular at least 2% by weight, more particularly at least 5% by weight, and especially at least 8% by weight,based on the total amount of mono-and oligosaccharides in the raw material, of one or more mono- or oligosaccharides different from 2′-FL, where the method comprises a)providing a solution of the 2′-FL raw material in water, which does not contain more than 10% by weight, preferably not more than 7% by weight, more preferably not more than 5% by weight of organic solvents, based on the total amount of water; b) effecting the crystallization of 2′-FL from the solution provided in step a) by inducing conditions of a controlled super saturation in the solution; and c) separating crystalline 2′-FL from the mother liquor, and where during controlled supersaturation in step b) not more than 10% by weight, preferably not more than 7% by weight, more preferably not more than 5% by weight of organic solvents are present, based on the total amount of water present during step b).

公开(公告)号：US20200181653A1

公开(公告)日：2020-06-11

申请号：US16614772

申请日：2018-05-18

Applicant: BASF SE

Inventor： Hartwig Schroeder ,  Stefan Haefner ,  Oskar Zelder ,  Christoph Wittmann ,  Anna Christine Schroer ,  Birgit Hoff ,  Nico Boehmer

IPC: C12P7/18 ,  C12N1/20

Abstract: Described herein is a process of producing an organic compound, the process including: I) cultivating a genetically modified microorganism in a culture medium including sucrose as an assimilable carbon source to allow the genetically modified microorganism to produce the organic compound, and II) recovering the organic compound from the fermentation broth obtained in process step I) The genetically modified microorganism includes A) at least one genetic modification that leads to an increased activity of the enzyme encoded by the rbsK-gene, compared to the original microorganism that has not been genetically modified, and the original microorganism belongs to the family Pasteurellaceae. Also described herein are a genetically modified microorganism and the use thereof for the fermentative production of an organic compound from sucrose as an assimilable carbon source.

公开(公告)号：US09598428B2

公开(公告)日：2017-03-21

申请号：US14760302

申请日：2014-01-15

Applicant: BASF SE

Inventor： Melanie Bonnekessel ,  Wolfgang Reichert ,  Ralf Hoock ,  Thomas Kaeding ,  Christopher Koradin ,  Andreas Pletsch ,  Manfred Ehresmann ,  Hartwig Schroeder

IPC: A01N43/90 ,  A01N53/00 ,  C07D493/04

CPC classification number: C07D493/04 ,  A01N43/90 ,  A01N53/00

Abstract: The present invention relates to a method for preparing the pyripyropene compound of the formula (I) which method comprises the following steps: i) subjecting a pyripyropene compound of formula Pyripyropene A to an alkaline hydrolysis to yield a 1,7,11 -trideacetylpyripyropene A, ii) reacting 1,7,11-trideacetylpyripyropene A obtained in step i) with cyclopropane carbonyl chloride to yield a raw product containing the pyripyropene compound of formula (I); iii) subjecting the raw product of step ii) to crystallization to yield a crystalline pyripyropene compound of formula (I) and a mother liquor; and iv) recycling the mother liquor or a pyripyropene compound containing fraction thereof to the alkaline hydrolysis of step i).

公开(公告)号：US20170073665A1

公开(公告)日：2017-03-16

申请号：US15308874

申请日：2015-05-07

Applicant: BASF SE

Inventor： Joanna Martyna Krawczyk ,  Stefan Haefner ,  Hartwig Schroeder ,  Oskar Zelder

IPC: C12N15/10 ,  C12N15/74 ,  C12P7/46

CPC classification number: C12N15/102 ,  C07K14/195 ,  C12N15/74 ,  C12N2510/02 ,  C12P7/46 ,  C12Y207/11

Abstract: The present invention relates to a modified microorganism having, compared to its wildtype,—a reduced activity of an enzyme encoded by the ptsA-gene,—a reduced activity of an enzyme encoded by the ptsH-gene or—a reduced activity of an enzyme encoded by the ptsA-gene and a reduced activity of an enzyme encoded by the ptsH-gene, wherein the wildtype from which the modified microorganism has been derived belongs to the family of Pasteurellaceae. The present invention also relates to a method for producing succinic acid and to the use of modified microorganisms.

Abstract translation: 本发明涉及一种经修饰的微生物，其与野生型相比具有降低的由ptsA基因编码的酶的活性， - 由ptsH基因编码的酶的活性降低或酶活性降低 由ptsA基因编码并且由ptsH基因编码的酶的活性降低，其中来源于修饰微生物的野生型属于巴氏杆菌科。 本发明还涉及生产琥珀酸的方法和使用改性微生物。