公开(公告)号：US20240327885A1

公开(公告)日：2024-10-03

申请号：US18561123

申请日：2022-05-17

申请人： DSM IP Assets B.V.

发明人： Manos PAPADAKIS ,  Ted JOHANSON ,  Peter BECKER

IPC分类号： C12P19/00 ,  C12N9/10

CPC分类号： C12P19/00 ,  C12N9/1051 ,  C12Y204/01086

摘要： The present disclosure relates to a method for the production of a human milk oligosaccharide (HMO) blend with LNFP-I and 2′-FL as the predominant HMO's, the method comprising the steps of providing a genetically engineered cell, which comprises a heterologous β-1,3-N-acetyl-glucosaminyltransferase protein, a heterologous β-1,3-galactosyltransferase protein, a heterologous α-1,2-fucosyltransferase protein, and expresses functionally the colanic acid gene cluster, comprises a native or heterologous regulatory or episomal element for controlling the expression of the proteins and optionally express a heterologous sugar transporter, and culturing the cell in a suitable cell culture medium to express said proteins and to produce an HMO blend.

公开(公告)号：US20240279696A1

公开(公告)日：2024-08-22

申请号：US17785813

申请日：2020-12-16

申请人： KYOWA HAKKO BIO CO., LTD.

发明人： Kenichiro TABATA ,  Shinsuke UCHIYA

IPC分类号： C12P19/00 ,  C12N9/12

CPC分类号： C12P19/00 ,  C12N9/1205 ,  C12Y207/01

摘要： An object of the invention is to provide a method for producing a lactose-containing oligosaccharide through more efficient fermentative production. According to the invention, using a microorganism having an ability of producing a protein which is modified by replacing a specific amino acid residue with a different amino acid residue and which has lactose permease activity, a lactose-containing oligosaccharide such as 2′-fucosyllactose can be produced more efficiently as compared to the case using a microorganism having an ability of producing the wild-type protein having lactose permease activity.

公开(公告)号：US20240247291A1

公开(公告)日：2024-07-25

申请号：US18559983

申请日：2022-05-11

申请人： The Regents of the University of California

发明人： Samanvaya Srivastava ,  Shang Gao ,  Advait Suhas Holkar

IPC分类号： C12P19/00 ,  A01N25/04 ,  A61K47/30 ,  B01J13/10 ,  C12N9/02 ,  C12N9/20

CPC分类号： C12P19/00 ,  A01N25/04 ,  A61K47/30 ,  B01J13/10 ,  C12N9/0008 ,  C12N9/20 ,  C12Y102/07 ,  C12Y301/01003

摘要： The invention described herein provides methods and materials that can impart long-term stability to polyelectrolyte complex coacervate droplets and create complex coacervate emulsions. The methodology described herein is designed to use one or more of a wide variety of comb polyelectrolytes in order to produce stable water-in-water emulsions with precisely controlled droplet size and enhanced stability profiles. The stabilized water-in-water emulsions microdroplets of the invention can further encapsulate active agents such as proteins and the like in a manner that protects them from the surrounding environment, thus allowing the compositions to serve as bio-microreactors and the like.

公开(公告)号：US11981947B2

公开(公告)日：2024-05-14

申请号：US17814333

申请日：2022-07-22

申请人： Chr. Hansen HMO GmbH

发明人： Stefan Jennewein

IPC分类号： C12P19/12 ,  C07H1/00 ,  C07H1/06 ,  C12N1/20 ,  C12N15/72 ,  C12P19/00 ,  C12P19/14 ,  C12P19/26 ,  C12R1/19

CPC分类号： C12P19/26 ,  C07H1/00 ,  C07H1/06 ,  C12N1/205 ,  C12N15/72 ,  C12P19/00 ,  C12P19/12 ,  C12P19/14 ,  C12Y302/01 ,  C12Y302/01023 ,  C12N2800/101 ,  C12R2001/19

摘要： The present invention relates to the use of one or more glycosidases in the process for the production and/or purification of a produced desired oligosaccharide. The process is preferably a microbial fermentation process using a host microorganism, which may also comprise nucleic acids expressing sugar catabolic pathway proteins suitable for the degradation of saccharides otherwise hindering the purification of the desired oligosaccharide.

公开(公告)号：US20240131500A1

公开(公告)日：2024-04-25

申请号：US18381837

申请日：2023-10-18

申请人： Griffin William Roberts ,  Robert K. Herrington ,  Trish Lyn Jackson ,  Alain Charles Briancon

发明人： Griffin William Roberts ,  Robert K. Herrington ,  Trish Lyn Jackson ,  Alain Charles Briancon

IPC分类号： B01J31/06 ,  B01J35/64 ,  C09K17/32 ,  C12P19/00

CPC分类号： B01J31/06 ,  B01J35/647 ,  C09K17/32 ,  C12P19/00

摘要： Provided is a methodology to improve soil performance by dispersing stabilized low C:N non-highly polymerized porous lignocellulose catalytic that improves porosity, carbon capture, and microbial activities.

公开(公告)号：US11872253B2

公开(公告)日：2024-01-16

申请号：US17724386

申请日：2022-04-19

申请人： Korea University Research and Business Foundation

发明人： Kyoung Heon Kim ,  Yong-Su Jin ,  Eun-Ju Yun ,  Dong-Hyun Kim ,  Sora Yu ,  Kyung-Mun Cho ,  Na Jeong Park

IPC分类号： C12P19/00 ,  A61K35/44 ,  A23L33/125 ,  A23L33/135 ,  A23L33/00 ,  A61K35/745 ,  A61K47/26 ,  A61K35/00

CPC分类号： A61K35/44 ,  A23L33/125 ,  A23L33/135 ,  A23L33/40 ,  A61K35/745 ,  A61K47/26 ,  C12P19/00 ,  A23V2002/00 ,  A23V2400/529 ,  A61K2035/115

摘要： The present invention relates to a method for producing marine algae-derived agarotriose, and a use thereof as a prebiotic. More specifically, the present invention investigates the characteristics of agarotriose as a prebiotic which is selectively metabolized by probiotic microorganisms, thereby enabling agarotriose to be used as an anti-cancer or anti-inflammatory agent in the fields of food and pharmaceuticals, and enabling agarotriose to be obtained at high yield through efficient purification with minimal loss after enzymatic hydrolysis of a red algae-derived polysaccharide without pre-treatment.

公开(公告)号：US11794145B1

公开(公告)日：2023-10-24

申请号：US17486992

申请日：2021-09-28

申请人： Nano BlueSkies Corp.

发明人： Lee Frelich ,  John MacDonald

IPC分类号： B01D53/62 ,  C12N1/20 ,  C12P19/00 ,  C12R1/01

CPC分类号： B01D53/62 ,  C12N1/20 ,  C12P19/00 ,  B01D2257/504 ,  C12R2001/01 ,  Y02P20/151

摘要： An atmospheric greenhouse gas reduction system that includes greenhouse gas reduction particles having carbon dioxide conversion material that is capable of converting carbon dioxide in atmosphere into simple carbohydrates. The greenhouse gas reduction particles have a size of up to about 1,000 nanometers.

公开(公告)号：US20230227876A1

公开(公告)日：2023-07-20

申请号：US17759273

申请日：2021-01-22

申请人： GLYCOM A/S

发明人： Margit PEDERSEN ,  Katrine Bych KAMPMANN

IPC分类号： C12P19/00 ,  C07K14/24

CPC分类号： C12P19/00 ,  C07K14/24

摘要： The present invention relates to the field of recombinant production of biological molecules in host cells. More particularly it relates to a method for recombinant production of human milk oligosaccharides (HMO) using a genetically modified cell expressing a protein of the major facilitator superfamily (MFS).

公开(公告)号：US11692211B2

公开(公告)日：2023-07-04

申请号：US16825176

申请日：2020-03-20

申请人： Comet Biorefining Inc.

发明人： Andrew Richard ,  Dennis D'Agostino

IPC分类号： C12P19/00 ,  C12P19/02 ,  C12P19/14 ,  D21C5/00 ,  C12N9/96 ,  C13K1/02 ,  C13K11/00 ,  C12N9/42 ,  D21C1/10 ,  C13K1/04 ,  C08B15/08

CPC分类号： C12P19/02 ,  C12N9/2437 ,  C12N9/96 ,  C12P19/14 ,  C12Y302/01004 ,  C13K1/02 ,  C13K1/04 ,  C13K11/00 ,  D21C1/10 ,  D21C5/005 ,  C08B15/08 ,  C12P2201/00

摘要： A two-step method for activating a cellulosic feedstock is described. The feedstock is subjected to a first high temperature activation step at a temperature greater than 190° C. and a second activation step at a lower temperature under alkali conditions. Also described are methods and compositions for the enzymatic hydrolysis of activated cellulose using one or more cellulase enzymes, a surfactant and polyaspartic acid. Also described are products of the methods.

公开(公告)号：US20230072639A1

公开(公告)日：2023-03-09

申请号：US17759267

申请日：2021-01-22

申请人： GLYCOM A/S

发明人： Margit PEDERSEN ,  Isotta D'ARRIGO ,  Katrine Bych KAMPMANN ,  Manos PAPADAKIS

IPC分类号： C12N5/10 ,  C12P19/00 ,  C12N15/74

摘要： The present invention relates to the field of recombinant production of biological molecules in genetically modified cells. More particularly, it relates to a method for recombinant production of human milk oligosaccharides (HMO) using a genetically modified cell expressing a protein of the major facilitator superfamily (MFS), the protein expressed being Bad.