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公开(公告)号：US20110059516A1
公开(公告)日：2011-03-10
申请号：US12942809
申请日：2010-11-09
CPC分类号： C12P7/04 , C07C403/08 , C07C403/24 , C07C2601/16 , C07D311/72 , C12N9/0004 , C12N9/0006 , C12N9/1085 , C12N15/81 , C12P9/00 , C12P17/06 , C12P23/00 , C12Y101/01034 , H01Q1/00
摘要： The invention provides a biological method of producing isoprenoids.
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公开(公告)号：US20110059515A1
公开(公告)日：2011-03-10
申请号：US12942723
申请日：2010-11-09
CPC分类号： C12P7/04 , C07C403/08 , C07C403/24 , C07C2601/16 , C07D311/72 , C12N9/0004 , C12N9/0006 , C12N9/1085 , C12N15/81 , C12P9/00 , C12P17/06 , C12P23/00 , C12Y101/01034 , H01Q1/00
摘要： The invention provides a biological method of producing isoprenoids.
摘要翻译： 本发明提供了生产类异戊二烯的生物学方法。
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公开(公告)号：US07544863B2
公开(公告)日：2009-06-09
申请号：US10862907
申请日：2004-06-07
申请人： Balasulojini Karunanandaa , Martha Post-Beittenmiller , Mylavarapu Venkatramesh , Ganesh M. Kishore , Gregory M. Thorne , John R. LeDeaux
发明人： Balasulojini Karunanandaa , Martha Post-Beittenmiller , Mylavarapu Venkatramesh , Ganesh M. Kishore , Gregory M. Thorne , John R. LeDeaux
CPC分类号： C12N9/0073 , C12N9/0006 , C12N9/0083 , C12N9/1007 , C12N15/8243 , C12N15/8247 , C12Y101/01034 , C12Y114/99007
摘要： Disclosed are constructs comprising sequences encoding 3-hydroxy-3methylglutaryl-Coenzyme A reductase and at least one other sterol synthesis pathway enzyme. Also disclosed are methods for using such constructs to alter sterol production and content in cells, plants, seeds and storage organs of plants. Also provided are oils and compositions containing altered sterol levels produced by use of the disclosed constructs. Novel nucleotide sequences useful in the alteration of sterol production are also provided. Also provided are cells, plants, seeds and storage organs of plants comprising sequences encoding 3-hydroxy-3methylglutaryl-Coenzyme A reductase, at least one other sterol synthesis pathway enzyme and at least one tocopherol synthesis enzyme.
摘要翻译： 公开了包含编码3-羟基-3甲基戊二酰辅酶A还原酶和至少一种其它甾醇合成途径酶的序列的构建体。 还公开了使用这种构建体来改变植物的细胞，植物，种子和储存器官中的固醇产生和含量的方法。 还提供了含有通过使用所公开的构建体产生的改变的固醇水平的油和组合物。 还提供了可用于改变甾醇生产的新型核苷酸序列。 还提供了包含编码3-羟基-3甲基戊二酰辅酶A还原酶，至少一种其它甾醇合成途径酶和至少一种生育酚合成酶的序列的植物的细胞，植物，种子和储存器官。
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公开(公告)号：US07232679B2
公开(公告)日：2007-06-19
申请号：US11129143
申请日：2005-05-13
申请人： Alan Berry , Werner Bretzel , Markus Hümbelin , Rual Lopez-Ulibarri , Anne Françoise Mayer , Alexei A. Yeliseev
发明人： Alan Berry , Werner Bretzel , Markus Hümbelin , Rual Lopez-Ulibarri , Anne Françoise Mayer , Alexei A. Yeliseev
IPC分类号： C12N9/88
CPC分类号： C12N9/0006 , C12N9/1022 , C12N9/1029 , C12N9/1085 , C12N9/1205 , C12N9/1229 , C12N9/88 , C12N9/90 , C12P23/00 , C12R1/01 , C12Y101/01034 , C12Y101/01036 , C12Y203/0301 , C12Y207/01036 , C12Y207/04002 , C12Y401/01033
摘要： Isolated polynucleotides encoding polypeptides having the activity of enzymes in the mevalonate pathway are provided. These sequences are useful for recombinantly producing isoprenoid compounds, such as carotenoids, in particular zeaxanthin. Expression vectors, cultured cells, and methods of making isoprenoid compounds are also provided.
摘要翻译： 提供了编码具有甲羟戊酸途径中的酶活性的多肽的分离的多核苷酸。 这些序列可用于重组产生类异戊二烯化合物，例如类胡萝卜素，特别是玉米黄质。 还提供了表达载体，培养细胞和制备类异戊二烯化合物的方法。
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公开(公告)号：US20050266518A1
公开(公告)日：2005-12-01
申请号：US11129143
申请日：2005-05-13
申请人： Alan Berry , Werner Bretzel , Markus Humbelin , Rual Lopez-Ulibarri , Anne Mayer , Alexei Yeliseev
发明人： Alan Berry , Werner Bretzel , Markus Humbelin , Rual Lopez-Ulibarri , Anne Mayer , Alexei Yeliseev
IPC分类号： C12N15/09 , C12N1/15 , C12N1/19 , C12N1/20 , C12N1/21 , C12N5/10 , C12N9/00 , C12N9/04 , C12N9/10 , C12N9/12 , C12N9/88 , C12N9/90 , C12N15/53 , C12N15/54 , C12N15/60 , C12N15/61 , C12P23/00 , C07H21/04 , C12N1/18 , C12N15/74 , C12P21/06
CPC分类号： C12N9/0006 , C12N9/1022 , C12N9/1029 , C12N9/1085 , C12N9/1205 , C12N9/1229 , C12N9/88 , C12N9/90 , C12P23/00 , C12R1/01 , C12Y101/01034 , C12Y101/01036 , C12Y203/0301 , C12Y207/01036 , C12Y207/04002 , C12Y401/01033
摘要： Isolated polynucleotides encoding polypeptides having the activity of enzymes in the mevalonate pathway are provided. These sequences are useful for recombinantly producing isoprenoid compounds, such as carotenoids, in particular zeaxanthin. Expression vectors, cultured cells, and methods of making isoprenoid compounds are also provided.
摘要翻译： 提供了编码具有甲羟戊酸途径中的酶活性的多肽的分离的多核苷酸。 这些序列可用于重组产生类异戊二烯化合物，例如类胡萝卜素，特别是玉米黄质。 还提供了表达载体，培养细胞和制备类异戊二烯化合物的方法。
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公开(公告)号：US20050086713A1
公开(公告)日：2005-04-21
申请号：US10862907
申请日：2004-06-07
申请人： Balasulojini Karunanandaa , Martha Post-Beittenmiller , Mylavarapu Venkatramesh , Ganesh Kishore , Gregory Thorne , John LeDeaux
发明人： Balasulojini Karunanandaa , Martha Post-Beittenmiller , Mylavarapu Venkatramesh , Ganesh Kishore , Gregory Thorne , John LeDeaux
CPC分类号： C12N9/0073 , C12N9/0006 , C12N9/0083 , C12N9/1007 , C12N15/8243 , C12N15/8247 , C12Y101/01034 , C12Y114/99007
摘要： Disclosed are constructs comprising sequences encoding 3-hydroxy-3methylglutaryl-Coenzyme A reductase and at least one other sterol synthesis pathway enzyme. Also disclosed are methods for using such constructs to alter sterol production and content in cells, plants, seeds and storage organs of plants. Also provided are oils and compositions containing altered sterol levels produced by use of the disclosed constructs. Novel nucleotide sequences useful in the alteration of sterol production are also provided. Also provided are cells, plants, seeds and storage organs of plants comprising sequences encoding 3-hydroxy-3methylglutaryl-Coenzyme A reductase, at least one other sterol synthesis pathway enzyme and at least one tocopherol synthesis enzyme.
摘要翻译： 公开了包含编码3-羟基-3甲基戊二酰辅酶A还原酶和至少一种其它甾醇合成途径酶的序列的构建体。 还公开了使用这种构建体来改变植物的细胞，植物，种子和储存器官中的固醇产生和含量的方法。 还提供了含有通过使用所公开的构建体产生的改变的固醇水平的油和组合物。 还提供了可用于改变甾醇生产的新型核苷酸序列。 还提供了包含编码3-羟基-3甲基戊二酰辅酶A还原酶，至少一种其它甾醇合成途径酶和至少一种生育酚合成酶的序列的植物的细胞，植物，种子和储存器官。
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公开(公告)号：US20030150008A1
公开(公告)日：2003-08-07
申请号：US09885723
申请日：2001-06-20
发明人： Balasulojini Karunanandaa , Martha Post-Beittenmiller , Mylavarapu Venkatramesh , Ganesh M. Kishore , Gregory M. Thorne , John R. LeDeaux
IPC分类号： A01H005/00 , C07H021/04 , C12N009/02
CPC分类号： C12N9/0073 , C12N9/0006 , C12N9/0083 , C12N9/1007 , C12N15/8243 , C12N15/8247 , C12Y101/01034 , C12Y114/99007
摘要： Disclosed are constructs comprising sequences encoding 3-hydroxy-3methylglutaryl-Coenzyme A reductase and at least one other sterol synthesis pathway enzyme. Also disclosed are methods for using such constructs to alter sterol production and content in cells, plants, seeds and storage organs of plants. Also provided are oils and compositions containing altered sterol levels produced by use of the disclosed constructs. Novel nucleotide sequences useful in the alteration of sterol production are also provided. Also provided are cells, plants, seeds and storage organs of plants comprising sequences encoding 3-hydroxy-3methylglutaryl-Coenzyme A reductase, at least one other sterol synthesis pathway enzyme and at least one tocopherol synthesis enzyme.
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公开(公告)号：US20030148416A1
公开(公告)日：2003-08-07
申请号：US10166225
申请日：2002-06-05
申请人： ROCHE VITAMINS INC.
发明人： Alan Berry , Werner Bretzel , Markus Humbelin , Rual Lopez-Ulibarri , Anne Francoise Mayer , Alexei A. Yeliseev
IPC分类号： C12P023/00 , C12P021/02 , C12N001/21 , C12N009/04 , C12N009/12 , C12N009/90 , C07H021/04 , C12N015/74
CPC分类号： C12N9/0006 , C12N9/1022 , C12N9/1029 , C12N9/1085 , C12N9/1205 , C12N9/1229 , C12N9/88 , C12N9/90 , C12P23/00 , C12R1/01 , C12Y101/01034 , C12Y101/01036 , C12Y203/0301 , C12Y207/01036 , C12Y207/04002 , C12Y401/01033
摘要： Isolated polynucleotides encoding polypeptides having the activity of enzymes in the mevalonate pathway are provided. These sequences are useful for recombinantly producing isoprenoid compounds, such as carotenoids, in particular zeaxanthin. Expression vectors, cultured cells, and methods of making isoprenoid compounds are also provided.
摘要翻译： 提供了编码具有甲羟戊酸途径中的酶活性的多肽的分离的多核苷酸。 这些序列可用于重组产生类异戊二烯化合物，例如类胡萝卜素，特别是玉米黄质。 还提供了表达载体，培养细胞和制备类异戊二烯化合物的方法。
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99.发明授权Method for making synthetic oligonucleotides which bind specifically to target sites on duplex DNA molecules, by forming a colinear triplex, the synthetic oligonucleotides and methods of use 失效通过形成共线三联制备合成寡核苷酸的方法，其特异性结合双链DNA分子上的靶位点，合成寡核苷酸和使用方法
公开(公告)号：US5176996A
公开(公告)日：1993-01-05
申请号：US453532
申请日：1989-12-22
CPC分类号： C12N15/113 , A61K48/00 , C07H21/00 , C07H21/04 , C12N15/1137 , C12N15/1138 , C12Q1/68 , C12Q1/6839 , C12Q1/6876 , C12Q1/6883 , C12Q1/6886 , C12Q1/703 , C12Q1/705 , C12Q1/708 , C12Y101/01034 , C12Y205/01018 , C12Y207/07007 , A61K38/00 , C12N2310/15 , C12N2310/321 , Y10T436/143333
摘要： A method for making synthetic oligonucleotides which bind to target sequences in a duplex DNA forming colinear triplexes by binding to the major groove. The method includes scanning genomic duplex DNA and identifying nucleotide target sequences of greater than about 20 nucleotides having either about at least 65% purine bases or about at least 65% pyrimidine bases; and synthesizing synthetic oligonucleotides complementary to identified target sequences. The synthetic oligonucleotides have a G when the complementary location in the DNA duplex has a GC base pair and have a T when the complementary location in the DNA duplex has an AT base pair. The synthetic oligonucleotides are oriented 5' to 4' and bind parallel or 3' to 5' and bind anti-parallel to the about at least 65% purine strand.
摘要翻译： 一种制备合成寡核苷酸的方法，其通过结合主要沟槽结合形成共线三联体的双链体DNA中的靶序列。 该方法包括扫描基因组双链DNA并鉴定大于约20个核苷酸的核苷酸靶序列，其具有约至少65％的嘌呤碱基或约至少65％的嘧啶碱基; 并合成与鉴定的靶序列互补的合成寡核苷酸。 当DNA双链体中的互补位置具有GC碱基对并且当DNA双链体中的互补位置具有AT碱基对时，合成的寡核苷酸具有G。 合成的寡核苷酸定向为5'至4'，并且平行或3'至5'结合并与大约至少65％的嘌呤链反平行结合。
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