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公开(公告)号：US09644231B2
公开(公告)日：2017-05-09
申请号：US14713887
申请日：2015-05-15
Applicant: Fluidigm Corporation
Inventor： Kenneth J. Livak , Stacey N. Meyers , Xiaohui Wang , Jun Wang
CPC classification number: C12Q1/6818 , C12Q2525/151
Abstract: The invention provides a method for detecting a target nucleotide sequence by tagging the nucleotide sequence with a nucleotide tag, providing a probe oligonucleotide with a melting temperature Tm1, comprising a regulatory sequence and a nucleotide tag recognition sequence; incorporating the probe oligonucleotide into the tagged polynucleotide in a polynucleotide amplification reaction, providing a regulatory oligonucleotide with a melting temperature Tm2, comprising a sequence segment that complementary to the regulatory sequence and a tail segment that does not hybridize to the probe nucleotide when the sequence segment and the regulatory sequence are annealed, amplifying the tagged target nucleic acid sequence in a PCR amplification reaction using the probe oligonucleotide as a primer, and using a DNA polymerase with high strand displacement activity and low 5′-nuclease activity, and detecting the amplification product; wherein Tm1 and Tm2 are higher than the annealing temperature associated with the polynucleotide amplification reaction.
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公开(公告)号：US09587272B2
公开(公告)日：2017-03-07
申请号：US14340446
申请日：2014-07-24
Applicant: Fluidigm Corporation
Inventor： Kenneth J. Livak , Stacey N. Meyers , Jun Wang , Xiaohui Wang
CPC classification number: C12Q1/6818 , C12Q2525/151
Abstract: The invention provides a method for detecting a target nucleotide sequence by tagging the nucleotide sequence with a nucleotide tag, providing a probe oligonucleotide with a melting temperature Tm1, comprising a regulatory sequence and a nucleotide tag recognition sequence; incorporating the probe oligonucleotide into the tagged polynucleotide in a polynucleotide amplification reaction, providing a regulatory oligonucleotide with a melting temperature Tm2, comprising a sequence segment that is at least partially complementary to the regulatory sequence, amplifying the tagged target nucleic acid sequence in a PCR amplification reaction using the probe oligonucleotide as a primer, and detecting the amplification product; wherein Tm1 and Tm2 are higher than the annealing temperature associated with the polynucleotide amplification reaction.
Abstract translation: 本发明提供了通过用核苷酸标签标记核苷酸序列来检测靶核苷酸序列的方法，提供了包含调节序列和核苷酸标签识别序列的具有解链温度Tm1的探针寡核苷酸; 在多核苷酸扩增反应中将探针寡核苷酸掺入标记的多核苷酸中，提供具有解链温度Tm2的调节性寡核苷酸，其包含与调节序列至少部分互补的序列片段，在PCR扩增中扩增标记的靶核酸序列 使用探针寡核苷酸作为引物的反应，检测扩增产物; 其中Tm1和Tm2高于与多核苷酸扩增反应相关的退火温度。
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公开(公告)号：US20170043340A1
公开(公告)日：2017-02-16
申请号：US15242187
申请日：2016-08-19
Applicant: Fluidigm Corporation
Inventor： JASON A. A. WEST , JESSE THOMPSON
CPC classification number: B01L3/502707 , B01J19/0046 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/00722 , B01L2200/0636 , B01L2200/0647 , B01L2200/0668 , B01L2200/0689 , B01L2300/0636 , B01L2300/0681 , B01L2300/0816 , B01L2300/0819 , B01L2300/0887 , B01L2300/12 , B29L2031/756 , G01N33/54386
Abstract: Provided are microfluidic devices and methods for fabricating and bonding such devices. Also provided are kits for analyzing analyte-containing samples and for lysing cells.
Abstract translation: 提供了用于制造和结合这种装置的微流体装置和方法。 还提供了用于分析含分析物的样品和裂解细胞的试剂盒。
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公开(公告)号：US09506914B2
公开(公告)日：2016-11-29
申请号：US14535162
申请日：2014-11-06
Applicant: Fluidigm Corporation
Inventor： Ian D. Manger , Joseph W. Barco , Hany R. Nassef
CPC classification number: B81B3/00 , B01L3/5025 , B01L3/502707 , B01L3/50273 , B01L3/502738 , B01L2300/0636 , B01L2300/0645 , B01L2300/0816 , B01L2300/0861 , B01L2300/088 , B01L2300/0887 , B01L2400/0415 , B01L2400/0481 , B01L2400/0655 , F04B19/006 , F04B43/02 , F16K99/0001 , F16K99/0003 , F16K99/0026 , F16K99/0036 , F16K99/0046 , F16K99/0051 , F16K99/0059 , F16K2099/0074 , F16K2099/0078 , F16K2099/008 , F16K2099/0084 , G01N33/5304 , G01N33/54366 , Y10T436/2575
Abstract: A microfluidic device includes a plurality of first flow channels and a plurality of second flow channels, each such second flow channel intersecting multiple of the first flow channels to define intersecting volumes and a plurality of looped flow channels that each include segments of the flow channels between the intersecting volumes to define a closed loop. The microfluidic device also includes a plurality of control valves each such control valve having a control channel and a deformable segment disposed to restrict flow through a respective one of the first and second flow channels in response to an actuation force applied to the control channel to deflect the deformable segment. The microfluidic device further includes a pump operatively disposed to regulate flow through one of the looped flow channels to regulate flow by the recirculating pump.
Abstract translation: 微流体装置包括多个第一流动通道和多个第二流动通道，每个这样的第二流动通道与第一流动通道的多个相交，以限定相交的体积，以及多个环形流动通道，每个流动通道包括在 相交的卷定义一个闭环。 微流体装置还包括多个控制阀，每个这样的控制阀具有控制通道和可变形部分，可变形部分设置成响应于施加到控制通道的致动力而限制流过第一和第二流动通道中的相应一个流动通道以偏转 可变形段。 微流体装置还包括一个可操作地设置成用于调节通过环形流动通道之一的流量以调节由再循环泵流动的泵。
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105.发明授权公开(公告)号：US09506845B2
公开(公告)日：2016-11-29
申请号：US13781318
申请日：2013-02-28
Applicant: Fluidigm Corporation
Inventor： Brian Fowler , Jake Kimball , Myo Thu Maung , Andrew May , Michael C. Norris , Dominique G. Toppani , Marc A. Unger , Jing Wang , Jason A. A. West
CPC classification number: G01N1/28 , B01L3/502761 , B01L7/52 , B01L2200/0668 , B01L2300/0864 , B01L2400/0409 , B01L2400/0415 , B01L2400/043 , B01L2400/0487 , B01L2400/086 , B01L2400/088 , C12P19/34 , C12Q1/6813 , C12Q1/6844 , C12Q1/686 , C12Q1/6869 , G01N1/34 , G01N15/1484 , C12Q2565/629
Abstract: Methods, systems, and devices are described for multiple single-cell capturing and processing utilizing microfluidics. Tools and techniques are provided for capturing, partitioning, and/or manipulating individual cells from a larger population of cells along with generating genetic information and/or reactions related to each individual cell. Different capture configurations may be utilized to capture individual cells and then processing each individual cell in a multi-chamber reaction configuration. Some embodiments may provide for specific target amplification, whole genome amplification, whole transcriptome amplification, real-time PCR preparation, copy number variation, preamplification, mRNA sequencing, and/or haplotyping of the multiple individual cells that have been partitioned from the larger population of cells. Some embodiments may provide for other applications. Some embodiments may be configured for imaging the individual cells or associated reaction products as part of the processing. Reaction products may be harvested and/or further analyzed in some cases.
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Patent Agency Ranking
公开(公告)号：US20160194685A1
公开(公告)日：2016-07-07
申请号：US14960754
申请日：2016-03-21
Applicant: Fluidigm Corporation
Inventor： Marc A. Unger , Geoffrey Richard Facer , Barry Clerkson , Christopher G. Cesar , Neil Switz
IPC: C12Q1/68
CPC classification number: C12Q1/686 , B01J2219/00576 , B01J2219/00704 , B01L3/5027 , B01L7/52 , G01N21/64 , G01N21/6452 , G01N21/6456 , G01N21/6486 , G01N21/75 , G01N21/8483 , G01N27/44721 , G01N2021/6421 , G01N2201/061 , G02B21/16 , G02B21/36
Abstract: An apparatus for imaging one or more selected fluorescence indications from a microfluidic device. The apparatus includes an imaging path coupled to least one chamber in at least one microfluidic device. The imaging path provides for transmission of one or more fluorescent emission signals derived from one or more samples in the at least one chamber of the at least one microfluidic device. The chamber has a chamber size, the chamber size being characterized by an actual spatial dimension normal to the imaging path. The apparatus also includes an optical lens system coupled to the imaging path. The optical lens system is adapted to transmit the one or more fluorescent signals associated with the chamber.
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公开(公告)号：US09381512B2
公开(公告)日：2016-07-05
申请号：US14071598
申请日：2013-11-04
Applicant: Fluidigm Corporation
Inventor： David S. Cohen , Jing Wang , Andrew May , Robert C. Jones , Hany Nassef
CPC classification number: B01L3/502738 , B01L3/5025 , B01L7/52 , B01L2200/0684 , B01L2300/0816 , B01L2300/0864 , B01L2300/087 , B01L2300/0874 , B01L2300/0887 , B01L2300/123 , B01L2400/0487 , B01L2400/0605 , B01L2400/0638 , B01L2400/0655 , B33Y80/00 , F16K99/0001 , F16K99/0026 , F16K99/0059 , F16K2099/0074 , F16K2099/0078 , F16K2099/008 , F16K2099/0084 , Y10T137/0318 , Y10T137/85938
Abstract: New high density microfluidic devices and methods provide precise metering of fluid volumes and efficient mixing of the metered volumes. A first solution is introduced into a segment of a flow channel in fluidic communication with a reaction chamber. A second solution is flowed through the segment so that the first solution is displaced into the reaction chamber, and a volume of the second solution enters the chamber. The chamber can then be isolated and reactions within the chamber can be initiated and/or detected. High throughput methods of genetic analysis can be carried out with greater accuracy than previously available.
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108.发明申请公开(公告)号：US20160017395A1
公开(公告)日：2016-01-21
申请号：US14800548
申请日：2015-07-15
Applicant: Fluidigm Corporation
Inventor： Peilin Chen , Jing Wang , Andrew May
IPC: C12P19/34
CPC classification number: C12P19/34 , C12Q1/6846 , C12Q1/6848 , C12Q2525/113 , C12Q2525/179 , C12Q2527/101 , C12Q2527/125 , C12Q2531/119
Abstract: The present invention provides reagents, kits, and methods for single-cell whole genome amplification using Phi 29 DNA polymerase.
Abstract translation: 本发明提供使用Phi 29 DNA聚合酶的单细胞全基因组扩增的试剂，试剂盒和方法。
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公开(公告)号：US20150337298A1
公开(公告)日：2015-11-26
申请号：US14719149
申请日：2015-05-21
Applicant: FLUIDIGM CORPORATION
Inventor： Lei Xi , Xiaohui Wang , Marc Unger , David Ruff
CPC classification number: C12N15/1082 , C12N15/1065 , C12Q1/6806 , C12Q2525/155 , C12Q2525/301 , C12Q2535/122
Abstract: In certain embodiments, the present invention provides a way of “digitally” marking different the alleles of different chromosomes by using a transposase to insert differently barcoded transposons into genomic DNA before further analysis. According to this method, each allele becomes marked with a unique pattern of transposon barcodes. Because each unique pattern of transposon barcodes identifies a particular allele, the method facilitates determinations of ploidy and copy number variation, improves the ability to discriminate among homozygotes, heterozygotes, and patterns arising from sequencing errors, and allows loci separated by uninformative stretches of DNA to be identified as linked loci, thereby facilitating haplotype determinations. Also provided is a novel artificial transposon end that includes a barcode sequence in two or more positions that are not essential for transposition.
Abstract translation: 在某些实施方案中，本发明提供了一种通过使用转座酶在进一步分析之前将不同条形码转座子插入基因组DNA中来“不同”地标记不同染色体等位基因的方式。 根据这种方法，每个等位基因都被标记为转座子条形码的独特模式。 因为转座子条形码的每个独特模式识别特定的等位基因，所以该方法有助于确定倍性和拷贝数变异，提高了鉴别纯合子，杂合子和测序错误引起的模式的能力，并且允许通过DNA的非信息延伸分离的位点 被确定为相关基因座，从而促进单倍型测定。 还提供了一种新颖的人工转座子末端，其包括对于转座不是必需的两个或更多个位置的条形码序列。
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公开(公告)号：US20150147755A1
公开(公告)日：2015-05-28
申请号：US14340446
申请日：2014-07-24
Applicant: Fluidigm Corporation
Inventor： Kenneth J. Livak , Stacey N. Myers , Jun Wang , Xiaohui Wang
IPC: C12Q1/68
CPC classification number: C12Q1/6818 , C12Q2525/151
Abstract: The invention provides a method for detecting a target nucleotide sequence by tagging the nucleotide sequence with a nucleotide tag, providing a probe oligonucleotide with a melting temperature Tm1, comprising a regulatory sequence and a nucleotide tag recognition sequence; incorporating the probe oligonucleotide into the tagged polynucleotide in a polynucleotide amplification reaction, providing a regulatory oligonucleotide with a melting temperature Tm2, comprising a sequence segment that is at least partially complementary to the regulatory sequence, amplifying the tagged target nucleic acid sequence in a PCR amplification reaction using the probe oligonucleotide as a primer, and detecting the amplification product; wherein Tm1 and Tm2 are higher than the annealing temperature associated with the polynucleotide amplification reaction.
Abstract translation: 本发明提供了通过用核苷酸标签标记核苷酸序列来检测靶核苷酸序列的方法，提供了具有调节序列和核苷酸标签识别序列的融解温度Tm1的探针寡核苷酸; 在多核苷酸扩增反应中将探针寡核苷酸掺入标记的多核苷酸中，提供具有解链温度Tm2的调节性寡核苷酸，其包含与调节序列至少部分互补的序列片段，在PCR扩增中扩增标记的靶核酸序列 使用探针寡核苷酸作为引物的反应，检测扩增产物; 其中Tm1和Tm2高于与多核苷酸扩增反应相关的退火温度。
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