公开(公告)号：US08715957B2

公开(公告)日：2014-05-06

申请号：US13191375

申请日：2011-07-26

申请人： Robin E. Osterhout ,  Anthony P. Burgard ,  Priti Pharkya ,  Mark J. Burk

发明人： Robin E. Osterhout ,  Anthony P. Burgard ,  Priti Pharkya ,  Mark J. Burk

IPC分类号： C12P1/00 ,  A61K38/00

CPC分类号： C12P7/62 ,  C12N15/52 ,  C12P5/005 ,  C12P5/026 ,  C12P7/16 ,  C12P7/40 ,  C12P9/00 ,  Y02E50/10

摘要： The invention provides non-naturally occurring microbial organisms having a toluene, benzene, p-toluate, terephthalate, (2-hydroxy-3-methyl-4-oxobutoxy)phosphonate, (2-hydroxy-4-oxobutoxy)phosphonate, benzoate, styrene, 2,4-pentadienoate, 3-butene-1ol or 1,3-butadiene pathway. The invention additionally provides methods of using such organisms to produce toluene, benzene, p-toluate, terephthalate, (2-hydroxy-3-methyl-4-oxobutoxy)phosphonate, (2-hydroxy-4-oxobutoxy)phosphonate, benzoate, styrene, 2,4-pentadienoate, 3-butene-1ol or 1,3-butadiene.

摘要翻译： 本发明提供了具有甲苯，苯，对甲苯甲酸酯，对苯二甲酸酯，（2-羟基-3-甲基-4-氧代丁氧基）膦酸酯，（2-羟基-4-氧代丁氧基）膦酸酯，苯甲酸酯，苯乙烯 ，2,4-戊二烯酸酯，3-丁烯-1醇或1,3-丁二烯途径。 本发明另外提供了使用这些生物体来生产甲苯，苯，对甲苯甲酸酯，对苯二甲酸酯，（2-羟基-3-甲基-4-氧代丁氧基）膦酸酯，（2-羟基-4-氧代丁氧基）膦酸酯，苯甲酸酯，苯乙烯 ，2,4-戊二烯酸酯，3-丁烯-1醇或1,3-丁二烯。

公开(公告)号：US20140113338A1

公开(公告)日：2014-04-24

申请号：US14009202

申请日：2012-03-30

申请人： Axel Christoph Trefzer ,  Stefanus Cornelis Hendrikus Josef Turk ,  Jan Metske Van Der Laan ,  Iise De Lange ,  Denise Ilse Jacobs

发明人： Axel Christoph Trefzer ,  Stefanus Cornelis Hendrikus Josef Turk ,  Jan Metske Van Der Laan ,  Iise De Lange ,  Denise Ilse Jacobs

IPC分类号： C12P13/00 ,  C12P7/44 ,  C12N9/88 ,  C12P7/40

CPC分类号： C12P13/001 ,  C08K5/544 ,  C08K5/5455 ,  C08L21/00 ,  C08L23/16 ,  C12N9/88 ,  C12P7/40 ,  C12P7/44 ,  C12Y401/01 ,  Y10T428/1352

摘要： The invention relates to an alpha-ketopimelic acid decarboxylase enzyme that is a homologue of SEQ ID NO:2, comprising at least one mutation selected from a group of substitutions listed in the specification, to a method for preparing 5-formyl valeric acid (hereinafter also referred to as ‘5-FVA’), to a method for preparing 6-aminocaproic acid (hereinafter also referred to as ‘6-ACA’), to a method for preparing ε-caprolactam (hereinafter referred to as ‘caprolactam’) from 6-ACA, to a method for the preparation of adipic acid, to a method for preparing diaminohexane. The invention further relates to a host cell which may be used in a method according to the invention and to a polynucleotide encoding an alpha-ketopimelic acid decarboxylase enzyme.

摘要翻译： 本发明涉及作为SEQ ID NO：2的同系物的α-酮己二酸酸脱羧酶，其包含选自本说明书中列出的取代基的至少一个突变与5-甲酰基戊酸的制备方法（下文 也称为“5-FVA”），涉及制备6-氨基己酸（以下也称为“6-ACA”）的方法，涉及制备ε-己内酰胺的方法（以下称为“己内酰胺” ）从6-ACA到制备己二酸的方法，涉及一种制备二氨基己烷的方法。 本发明还涉及可用于根据本发明的方法的宿主细胞和编码α-酮己糖酸脱羧酶的多核苷酸。

公开(公告)号：US08703451B2

公开(公告)日：2014-04-22

申请号：US13002519

申请日：2009-04-29

申请人： Thomas Haas ,  Thomas Tacke ,  Achim Marx ,  Alexander Schraven ,  Olivier Zehnacker ,  Eva Maria Wittmann

发明人： Thomas Haas ,  Thomas Tacke ,  Achim Marx ,  Alexander Schraven ,  Olivier Zehnacker ,  Eva Maria Wittmann

IPC分类号： C12P7/40 ,  C07C51/47 ,  C07C51/41 ,  C12P7/42

CPC分类号： C12P7/40 ,  C07C51/02 ,  C07C51/41 ,  C07C51/47 ,  C12P7/42 ,  C07C59/01 ,  C07C59/06 ,  C07C59/255 ,  C07C59/08 ,  C07C59/265

摘要： A process for the preparation of free carboxylic acids including: A) preparation of carboxylic acid by a biological cell located in an aqueous medium with addition of an amine of formula (I) where R1, R2 and R3, independently of one another, are identical or different, branched or unbranched, optionally substituted hydrocarbon radicals or H; B) for cases where the added amine A) is water-soluble, addition of a water-insoluble amine of formula (I), where, in A) or B), a multiphase system is obtained and the corresponding ammonium carboxylate is formed from the water-insoluble amine and the carboxylic acid; C) removal of the water-insoluble phase; and D) heating of the water-insoluble phase with release of free carboxylic acid.

摘要翻译： 一种制备游离羧酸的方法，包括：A）通过加入式（I）的胺的位于水性介质中的生物细胞制备羧酸，其中R1，R2和R3彼此独立地相同 或不同的支链或非支链的任选取代的烃基或H; B）对于添加的胺A）是水溶性的，加入式（I）的水不溶性胺，其中在A）或B）中获得多相体系，并且相应的羧酸铵由 水不溶性胺和羧酸; C）去除水不溶性相; 和D）释放游离羧酸时加热水不溶性相。

公开(公告)号：US20140051137A1

公开(公告)日：2014-02-20

申请号：US13838570

申请日：2013-03-15

申请人： Butamax(TM) Advanced Biofuels LLC

发明人： Dennis FLINT ,  Steven Cary Rothman ,  Wonchul Suh ,  Jean-Francois Tomb ,  Rick W. Ye

IPC分类号： C12N9/88 ,  C12P7/16 ,  G06F19/22

CPC分类号： G16B30/00 ,  C12N9/88 ,  C12P7/16 ,  C12P7/40 ,  C12P13/06 ,  C12P13/08 ,  C12Y402/01009 ,  Y02E50/10

摘要： A group of bacterial dihydroxy-acid dehydratases having a [2Fe-2S] cluster was discovered. Bacterial [2Fe-2S] DHADs were expressed as heterologous proteins in bacteria and yeast cells, providing DHAD activity for conversion of 2,3-dihydroxyisovalerate to α-ketoisovalerate or 2,3-dihydroxymethylvalerate to α-ketomethylvalerate. Isobutanol and other compounds may be synthesized in pathways that include bacterial [2Fe-2S] DHAD activity.

摘要翻译： 发现一组具有[2Fe-2S]簇的细菌二羟基酸脱水酶。 细菌[2Fe-2S] DHADs在细菌和酵母细胞中表达为异源蛋白质，提供DHAD活性，用于将2,3-二羟基异戊酸酯转化为α-酮异戊酸酯或2,3-二羟甲基戊酸酯转化为α-酮基甲基戊酸酯。 异丁醇和其他化合物可以在包括细菌[2Fe-2S] DHAD活性的途径中合成。

公开(公告)号：US20140038263A1

公开(公告)日：2014-02-06

申请号：US13837921

申请日：2013-03-15

申请人： Butamax(TM) Advanced Biofuels LLC

发明人： Dennis FLINT ,  Brian James Paul ,  Rick W. Ye

IPC分类号： C12N9/88

CPC分类号： C12P7/16 ,  C07K14/395 ,  C12N9/88 ,  C12N15/81 ,  C12P7/40 ,  C12P13/02 ,  C12P13/04 ,  C12Q1/527 ,  C12Y402/01009 ,  G01N33/573 ,  Y02E50/10 ,  Y02P20/52

摘要： The present invention is related to a recombinant host cell, in particular a yeast cell, comprising a dihydroxy-acid dehydratase polypeptide. The invention is also related to a recombinant host cell having increased specific activity of the dihydroxy-acid dehydratase polypeptide as a result of increased expression of the polypeptide, modulation of the Fe—S cluster biosynthesis of the cell, or a combination thereof. The present invention also includes methods of using the host cells, as well as, methods for identifying polypeptides that increase the flux in an Fe—S cluster biosynthesis pathway in a host cell.

公开(公告)号：US20140038254A1

公开(公告)日：2014-02-06

申请号：US13954294

申请日：2013-07-30

申请人： The United States of America, as represented by the Secretary of Agriculture ,  Ion Research, Inc.

发明人： Anthony B. Kuhry ,  Paul J. Weimer

IPC分类号： C12P5/02

CPC分类号： C25B3/04 ,  C12N1/22 ,  C12P3/00 ,  C12P5/02 ,  C12P5/023 ,  C12P5/026 ,  C12P7/40 ,  C12P7/52 ,  C12P7/54 ,  C12P7/6409 ,  C12P39/00 ,  C25B1/02 ,  Y02E50/343

摘要： Hydrocarbon and hydrogen fuels and other products may be produced by a process employing a combination of fermentation and electrochemical stages. In the process, a biomass contained within a fermentation medium is fermented with an inoculum comprising a mixed culture of microorganisms derived the rumen contents of a rumen-containing animal. This inoculated medium is incubated under anaerobic conditions and for a sufficient time to produce volatile fatty acids. The resultant volatile fatty acids are then subjected to electrolysis under conditions effective to convert said volatile fatty acids to hydrocarbons and hydrogen simultaneously. The process can convert a wide range of biomass materials to a wide range of volatile fatty acid chain lengths and can convert these into a wide range of biobased fuels and biobased products.

公开(公告)号：US08637284B2

公开(公告)日：2014-01-28

申请号：US13312753

申请日：2011-12-06

申请人： Marshall Medoff

发明人： Marshall Medoff

IPC分类号： C12P1/02 ,  C12N9/42 ,  B01J19/08 ,  B60H1/00 ,  C08B1/00 ,  C07C4/00 ,  A23L3/26 ,  D21C5/00

CPC分类号： C12P13/24 ,  A23K10/12 ,  A23K10/30 ,  A23K10/37 ,  A23K20/163 ,  A23K40/00 ,  A23K50/10 ,  A23K50/80 ,  A23L5/36 ,  A23L7/115 ,  A23V2002/00 ,  A61K8/97 ,  A61Q19/00 ,  B01D39/18 ,  C08H8/00 ,  C08J3/28 ,  C08L1/02 ,  C08L5/14 ,  C12P7/10 ,  C12P7/16 ,  C12P7/40 ,  C12P7/46 ,  C12P7/56 ,  C12P13/04 ,  C12P13/06 ,  C12P13/08 ,  C12P13/12 ,  C12P13/14 ,  C12P13/20 ,  C12P13/222 ,  C12P13/227 ,  C12P19/02 ,  C12P19/14 ,  C12P19/44 ,  C12P2201/00 ,  C12P2203/00 ,  C13K1/02 ,  D21C5/005 ,  D21C9/001 ,  Y02A40/818 ,  Y02E50/10 ,  Y02E50/16 ,  Y02E50/17 ,  Y02E50/30 ,  Y02P60/877

摘要： Biomass (e.g., plant biomass, animal biomass, microbial, and municipal waste biomass) is processed to produce useful products, such as food products and amino acids.

摘要翻译： 加工生物质（例如，植物生物量，动物生物量，微生物和城市废物生物质）以产生有用的产物，例如食品和氨基酸。

公开(公告)号：US20130316426A1

公开(公告)日：2013-11-28

申请号：US13887140

申请日：2013-05-03

申请人： Genomatica, Inc.

发明人： Mark J. Burk ,  Priti Pharkya ,  Stephen J. Van Dien ,  Anthony P. Burgard ,  Christophe H. Schilling

IPC分类号： C07C51/38 ,  C07C67/32

CPC分类号： C07C51/353 ,  C07C51/38 ,  C07C57/04 ,  C07C67/08 ,  C07C67/32 ,  C07C67/333 ,  C12P7/40 ,  C12P7/46 ,  C12P7/62 ,  Y02P20/125 ,  C07C69/60 ,  C07C69/54

摘要： The invention provides a method of producing acrylic acid. The method includes contacting fumaric acid with a sufficient amount of ethylene in the presence of a cross-metathesis transformation catalyst to produce about two moles of acrylic acid per mole of fumaric acid. Also provided is an acrylate ester. The method includes contacting fumarate diester with a sufficient amount of ethylene in the presence of a cross-metathesis transformation catalyst to produce about two moles of acrylate ester per mole of fumarate diester. An integrated process for process for producing acrylic acid or acrylate ester is provided which couples bioproduction of fumaric acid with metathesis transformation. An acrylic acid and an acrylate ester production also is provided.

摘要翻译： 本发明提供了制备丙烯酸的方法。 该方法包括在交叉复分解转化催化剂存在下使富马酸与足够量的乙烯接触，以产生约2摩尔丙烯酸/摩尔富马酸。 还提供了丙烯酸酯。 该方法包括在交叉复分解转化催化剂存在下使富马酸二酯与足够量的乙烯接触，以产生每摩尔富马酸二酯约2摩尔丙烯酸酯。 提供了用于生产丙烯酸或丙烯酸酯的方法的综合方法，其将富马酸的生物生产与易位转化相结合。 还提供丙烯酸和丙烯酸酯生产。

公开(公告)号：US20130295624A1

公开(公告)日：2013-11-07

申请号：US13935065

申请日：2013-07-03

申请人： Xyleco

发明人： Marshall Medoff ,  Thomas Craig Masterman

IPC分类号： C12P7/64 ,  C12P7/40 ,  C12P5/02 ,  C12P7/18 ,  C12P7/06 ,  C12P7/46 ,  C12P7/20 ,  C12P7/54 ,  C12P7/52 ,  C12P7/58 ,  C12P7/56 ,  C12P7/42 ,  C12P7/04 ,  C12P7/16

CPC分类号： C12P7/10 ,  C07H3/02 ,  C07H3/04 ,  C12N1/20 ,  C12N1/38 ,  C12P5/02 ,  C12P7/04 ,  C12P7/06 ,  C12P7/16 ,  C12P7/18 ,  C12P7/20 ,  C12P7/40 ,  C12P7/42 ,  C12P7/46 ,  C12P7/52 ,  C12P7/54 ,  C12P7/56 ,  C12P7/58 ,  C12P7/6409 ,  C12P19/02 ,  C12P2201/00 ,  C12P2203/00 ,  Y02E50/16 ,  Y02E50/17 ,  Y02E50/343

摘要： Biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) is processed to produce useful products, such as fuels. For example, systems can use feedstock materials, such as cellulosic and/or lignocellulosic materials, to produce ethanol and/or butanol, e.g., by fermentation.

公开(公告)号：US20130289113A1

公开(公告)日：2013-10-31

申请号：US13799882

申请日：2013-03-13

申请人： E. I. du Pont de Nemours and Company

发明人： Mark Scott PAYNE ,  Robert DiCosimo

IPC分类号： C12N9/18

CPC分类号： C12N9/18 ,  A61K8/38 ,  A61K8/66 ,  A61K2800/10 ,  A61K2800/805 ,  A61Q5/02 ,  A61Q11/00 ,  A61Q17/005 ,  A61Q19/00 ,  A61Q19/10 ,  C11D3/2093 ,  C11D3/221 ,  C11D3/226 ,  C11D3/38636 ,  C12P7/40

摘要： Acetyl xylan esterases and variants thereof having perhydrolytic activity are provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. Multi-component peracid generation systems comprising an enzyme catalyst having perhydrolytic activity are also provided, as are methods of using the present enzyme catalyst to produce peroxycarboxylic acids. The polypeptide having perhydrolytic activity may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, paper pulp processing, and personal care applications.