公开(公告)号：US20190040457A1

公开(公告)日：2019-02-07

申请号：US15758814

申请日：2016-05-12

Applicant: WAKE FOREST UNIVERSITY HEALTH SCIENCES

Inventor： ADAM R. HALL

IPC: C12Q1/6827 ,  C12P19/34 ,  C12Q1/6869 ,  C07H21/04 ,  C12N15/10 ,  C12Q1/6834

Abstract: Described are methods of detecting modified nucleotide bases in a DNA sample using specific DNA glycosylases to excise target modified bases. DNA molecules are then labeled using a DNA polymerase lacking 3′→5′ exo-nuclease activity and strand displacement activity. The methods can be used to detect epigenetic changes and DNA damage. Provided are methods for diagnosing a disease or condition, determining risk of a disease or condition, identifying appropriate treatment, monitoring effectiveness of treatment, and monitoring side effects of treatment in subjects based on detection of modified bases. Also provided are methods for determining environmental exposure, or an environmental exposure time, of a biological sample containing DNA. Also provided are kits, systems, and devices for performing the described methods.

公开(公告)号：US20190038849A1

公开(公告)日：2019-02-07

申请号：US16158808

申请日：2018-10-12

Applicant: Wake Forest University Health Sciences

Inventor： James J. Yoo ,  Anthony Atala ,  Kyle W. Binder ,  Mohammad Z. Albanna ,  Weixin Zhao ,  Dennis Dice ,  Tao Xu

IPC: A61M11/00 ,  A61L27/38 ,  A61L27/60 ,  A61B5/00 ,  A61B5/107 ,  A61L27/22 ,  C12N5/077 ,  A61M35/00 ,  A61L27/24 ,  A61B34/30 ,  A61B90/50 ,  A61B17/00 ,  A61B17/322 ,  A61B34/10 ,  B41J3/407

Abstract: Provided herein is a delivery system, including: (a) an optical sensor configured to detect data to create a map of a patient bodily surface; and (b) a dispenser operatively associated with the optical sensor and configured to deliver compositions (optionally including cells) to the patient bodily surface based upon the data or map. Methods of forming a tissue on a patient bodily surface of a patient in need thereof are also provided, as are methods, systems and computer program products useful for processing patient bodily surface data.

公开(公告)号：US20180291350A1

公开(公告)日：2018-10-11

申请号：US15768100

申请日：2016-10-14

Applicant: Wake Forest University Health Sciences

Inventor： Sean V. Murphy ,  Anthony Atala

IPC: C12N5/071 ,  C12M3/00 ,  C12M3/06 ,  C12M1/00 ,  C12N1/20

Abstract: Provided herein are artificial lung organoids. The artificial lung organoids may include an epithelial cell layer comprising mammalian lung epithelial cells, a stromal cell layer comprising mammalian lung fibroblast cells and an endothelial cell layer comprising mammalian endothelial cells. The artificial lung organoids may optionally include a porous membrane between said epithelial cell layer and said stromal cell layer and/or between said stromal cell layer and said endothelial lung cell layer.

公开(公告)号：US10092679B2

公开(公告)日：2018-10-09

申请号：US14518352

申请日：2014-10-20

Applicant: Wake Forest University Health Sciences

Inventor： Hyunhee Ahn ,  Young Min Ju ,  Anthony Atala ,  Sang Jin Lee

IPC: A61L27/50 ,  A61L27/26 ,  A61F2/07 ,  A61L27/38 ,  A61F2/06 ,  C12N5/071 ,  D01D5/00

Abstract: Vascular scaffolds and methods of fabricating the same are disclosed for tissue engineering of vascular constructs. By combining electrospun matrices with cell sheet technologies, vascular constructs with more mature cell layers can be obtained for reconstruction of blood vessels, heart valves and the like. A engineered smooth muscle cell sheet, wrapped around an electrospun vascular scaffold, is able to provide a mature SMC layer that expresses strong cell-to-cell junction markers and contractile proteins. In addition, preconditioning of the cell sheet covered vascular scaffold maintained cell viability and infiltration into the scaffold.

公开(公告)号：US20180133422A1

公开(公告)日：2018-05-17

申请号：US15578453

申请日：2015-06-16

Applicant: Wake Forest University Health Sciences

Inventor： Michael A. Olympio ,  Philip J. Brown

IPC: A61M16/04 ,  A61B1/267

CPC classification number: A61M16/049 ,  A61B1/267 ,  A61M16/0488 ,  A61M16/0493 ,  A61M16/0495 ,  A61M2025/0183 ,  A61M2209/04

Abstract: Tracheal tube devices include: (a) a mouthpiece sized and configured to allow an endotracheal tube to extend outwardly therefrom; and (b) a handle holding a cutting member. The mouthpiece can include at least one outwardly projecting substantially rigid short tube. The cutting member may be held in a handle releasably attached to the short tube.

公开(公告)号：US09931434B2

公开(公告)日：2018-04-03

申请号：US14357443

申请日：2012-11-09

Applicant: Trustees of Tufts College ,  University of Pittsburgh—of the Commonwealth System of Higher Education ,  Wake Forest University Health Sciences

Inventor： Evangelia Bellas ,  Kacey Marra ,  J. Peter Rubin ,  David L. Kaplan ,  James J. Yoo

IPC: A61L27/22 ,  A61L27/54 ,  A61L27/36 ,  A61L27/38 ,  A61L27/48 ,  A61L27/56 ,  A61L27/58

CPC classification number: A61L27/227 ,  A61L27/3604 ,  A61L27/3834 ,  A61L27/48 ,  A61L27/54 ,  A61L27/56 ,  A61L27/58 ,  A61L2400/06 ,  A61L2430/34 ,  Y10T428/2982 ,  C08L89/00

Abstract: The inventions provided herein relate to compositions, methods, delivery devices and kits for repairing or augmenting a tissue in a subject. The compositions described herein are injectable such that they can be placed in a tissue to be treated with a minimally-invasive procedure (e.g., by injection) and/or be molded flexibly into a tissue void of any shape and/or size. In some embodiments, the composition described herein comprises a plurality of silk fibroin particles, which can retain their original volume within the tissue for a period of time. The compositions can be used as a filler to replace a tissue void, e.g., for tissue repair and/or augmentation, or as a scaffold to support tissue regeneration and/or reconstruction. In some embodiments, the compositions described herein can be used for soft tissue repair or augmentation.

公开(公告)号：US09896656B2

公开(公告)日：2018-02-20

申请号：US14216391

申请日：2014-03-17

Applicant: Wake Forest University Health Sciences

Inventor： Khalil Bitar

IPC: C12N5/00 ,  C12N5/02 ,  C12N5/0793 ,  C12N5/079 ,  A61K35/30 ,  A61K35/34

CPC classification number: C12N5/0619 ,  A61K35/30 ,  A61K35/34 ,  C12N5/0622 ,  C12N2502/1347 ,  C12N2533/52 ,  C12N2533/54 ,  C12N2533/90

Abstract: Differentiation and stability of neural stem cells can be enhanced by in vitro or in vivo culturing with one or more extracellular matrix (ECM) compositions, such as collagen I, IV, laminin and/or a heparan sulfate proteoglycan. In one aspect of the invention, adult mammalian enteric neuronal progenitor cells can be induced to differentiate on various substrates derived from components or combinations of neural ECM compositions. Collagen I and IV supported neuronal differentiation and extensive glial differentiation individually and in combination. Addition of laminin or heparan sulfate to collagen substrates unexpectedly improved neuronal differentiation, increasing neuron number, branching of neuronal processes, and initiation of neuronal network formation. In another aspect, neuronal subtype differentiation was affected by varying ECM compositions in hydrogels overlaid on intestinal smooth muscle sheets. The matrix compositions of the present invention can be used to tissue engineer transplantable innervated GI smooth muscle constructs to remedy aganglionic disorders.

公开(公告)号：US09878013B2

公开(公告)日：2018-01-30

申请号：US15045557

申请日：2016-02-17

Applicant: Wake Forest University Health Sciences

Inventor： Waldemar Debinski ,  Hetal Pandya ,  Denise Mazess Herpai

IPC: A61K38/20 ,  A61K47/48 ,  C07K7/06 ,  C07K7/08 ,  A61K38/08 ,  A61K38/10 ,  C07K14/54 ,  A61K38/00

CPC classification number: A61K38/2086 ,  A61K38/00 ,  A61K38/08 ,  A61K38/10 ,  A61K47/64 ,  A61K47/6415 ,  C07K7/06 ,  C07K7/08 ,  C07K14/5437 ,  C07K2319/01 ,  C07K2319/55

Abstract: A targeting peptide that specifically binds to an IL13 receptor (e.g., wherein said targeting peptide is not an IL13 fragment) is described. The targeting peptide is optionally conjugated to at least one effector molecule. In some embodiments, the peptide specifically binds to the IL13Rα2 protein.

公开(公告)号：US20170354691A1

公开(公告)日：2017-12-14

申请号：US15689268

申请日：2017-08-29

Applicant: Wake Forest University Health Sciences

Inventor： Emmanuel C. Opara ,  Benjamin S. Harrison

IPC: A61K35/39 ,  A61K9/50 ,  A61K47/22 ,  A61K38/44 ,  A61K47/02 ,  A61K38/18 ,  A61K9/00 ,  C12N1/04 ,  A61K45/06 ,  A61K47/42 ,  C12N11/10 ,  C12N11/04 ,  A61K33/40 ,  C12N5/071 ,  A61K31/555

CPC classification number: A61K35/39 ,  A61K9/0024 ,  A61K9/501 ,  A61K9/5015 ,  A61K9/5036 ,  A61K9/5073 ,  A61K31/555 ,  A61K33/40 ,  A61K38/1866 ,  A61K38/44 ,  A61K45/06 ,  A61K47/02 ,  A61K47/22 ,  A61K47/42 ,  C12N1/04 ,  C12N5/0676 ,  C12N11/04 ,  C12N11/10

Abstract: Microcapsules are described that comprise (a) a liquid aqueous or hydrogel core; (b) a semipermeable membrane surrounding said core; (c) live animal cells (e.g., pancreatic cells) in the core; and (d) oxygen-generating particles in said core, said oxygen-generating particles included in said microcapsules in an amount sufficient to lengthen the duration of viability of said animal cells in said microcapsules. Compositions comprising such microcapsules and uses thereof, such as in treating diabetes, are also described.

公开(公告)号：US09801713B2

公开(公告)日：2017-10-31

申请号：US14987601

申请日：2016-01-04

Applicant: Wake Forest University Health Sciences

Inventor： Anthony Atala ,  James Yoo

IPC: C12N5/00 ,  A61F2/24 ,  C12N5/071 ,  A61L27/36 ,  A61L27/38 ,  A61L27/50 ,  B82Y5/00 ,  B82Y10/00

CPC classification number: A61F2/2415 ,  A61L27/3604 ,  A61L27/3641 ,  A61L27/3808 ,  A61L27/3826 ,  A61L27/383 ,  A61L27/3839 ,  A61L27/3886 ,  A61L27/507 ,  B82Y5/00 ,  B82Y10/00 ,  C12N5/0068 ,  C12N5/069 ,  C12N2533/90

Abstract: The invention is directed to methods for preparing artificial heart valves by preconditioning a matrix seeded with endothelial cells and smooth muscle cells differentiated from isolated progenitor cells. These cell seeded matrices are exposed to fluid conditions that mimic blood flow through the heart to produce tissue engineered heart valves that are analogous to native heart valves.