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151.发明申请公开(公告)号：US20140272952A1
公开(公告)日：2014-09-18
申请号：US14180262
申请日：2014-02-13
Applicant: Fluidigm Corporation
Inventor： Andrew May , Peilin Chen , Jun Wang , Fiona Kaper , Megan Anderson
IPC: C12Q1/68
CPC classification number: C12Q1/6806 , B01L3/50273 , B01L3/502738 , B01L7/52 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2300/087 , B01L2400/0487 , B01L2400/0655 , C12Q1/686 , C12Q2525/155 , C12Q2525/161 , C12Q2527/143 , C12Q2535/122 , C12Q2549/119 , C12Q2563/179 , C12Q2565/629
Abstract: In certain embodiments, the present invention provides amplification methods in which nucleotide tag(s) and, optionally, a barcode nucleotide sequence are added to target nucleotide sequences. In other embodiments, the present invention provides a microfluidic device that includes a plurality of first input lines and a plurality of second input lines. The microfluidic device also includes a plurality of sets of first chambers and a plurality of sets of second chambers. Each set of first chambers is in fluid communication with one of the plurality of first input lines. Each set of second chambers is in fluid communication with one of the plurality of second input lines. The microfluidic device further includes a plurality of first pump elements in fluid communication with a first portion of the plurality of second input lines and a plurality of second pump elements in fluid communication with a second portion of the plurality of second input lines.
Abstract translation: 在某些实施方案中，本发明提供了其中将核苷酸标签和任选的条形码核苷酸序列加入靶核苷酸序列的扩增方法。 在其它实施例中，本发明提供一种包括多个第一输入线和多个第二输入线的微流体装置。 微流体装置还包括多组第一腔室和多组第二腔室。 每组第一腔室与多个第一输入管线中的一个流体连通。 每组第二腔室与多个第二输入管线中的一个流体连通。 微流体装置还包括与多个第二输入线的第一部分流体连通的多个第一泵元件和与多个第二输入线的第二部分流体连通的多个第二泵元件。
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公开(公告)号：US20140193812A1
公开(公告)日：2014-07-10
申请号：US14102331
申请日：2013-12-10
Applicant: Fluidigm Corporation
Inventor： Amy Hamilton , Min Lin , Alain Mir , Martin Pieprzyk
IPC: C12Q1/68
CPC classification number: C12Q1/6881 , C12Q1/6844 , C12Q1/686 , C12Q2531/113 , C12Q2531/119 , C12Q2531/137 , C12Q2565/101 , C12Q2600/156 , C12Q2600/158 , C12Q2600/16 , C12Q2600/178
Abstract: The present invention provides methods for analysis of genomic DNA and/or RNA from small samples or even single cells. Methods for analyzing genomic DNA can entail whole genome amplification (WGA), followed by preamplification and amplification of selected target nucleic acids. Methods for analyzing RNA can entail reverse transcription of the desired RNA, followed by preamplification and amplification of selected target nucleic acids.
Abstract translation: 本发明提供从小样品甚至单细胞分析基因组DNA和/或RNA的方法。 分析基因组DNA的方法可能需要全基因组扩增（WGA），然后对选定的靶核酸进行预扩增和扩增。 分析RNA的方法可能需要所需RNA的逆转录，然后对选定的靶核酸进行预扩增和扩增。
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公开(公告)号：US20140154787A1
公开(公告)日：2014-06-05
申请号：US13915476
申请日：2013-06-11
Applicant: Fluidigm Corporation
Inventor： Gang Sun , Ramesh Ramakrishnan , Robert C. Jones
IPC: C12Q1/68
CPC classification number: C12Q1/686 , C12Q2565/629 , C12Q2565/501 , C12Q2545/114
Abstract: A method of adjusting amplification curves in a PCR experiment includes receiving a plurality of amplification curves for a sample and computing a first parameter for each of the plurality of amplification curves. The method also includes computing a second parameter for each of the plurality of amplification curves and computing a third parameter using at least a portion of the first or second parameters. The method further includes computing an offset for each of the plurality of amplification curves. The offset is a function of the first parameter and the third parameter. Moreover, the method includes adjusting at least one of the plurality of amplification curves by subtracting the offset.
Abstract translation: 在PCR实验中调整扩增曲线的方法包括接收样品的多个放大曲线并计算多个放大曲线中的每一个的第一参数。 该方法还包括为多个放大曲线中的每一个计算第二参数，并使用第一或第二参数的至少一部分来计算第三参数。 该方法还包括计算多个放大曲线中的每一个的偏移量。 偏移量是第一个参数和第三个参数的函数。 此外，该方法包括通过减去偏移来调整多个放大曲线中的至少一个。
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公开(公告)号：US20140133732A1
公开(公告)日：2014-05-15
申请号：US14071423
申请日：2013-11-04
Applicant: Fluidigm Corporation
Inventor： Simant Dube , Gang Sun , Lian-She Zhao
IPC: G01N21/64
CPC classification number: G01N21/64 , G06T7/0012 , G06T7/74 , G06T2207/10064 , G06T2207/30004
Abstract: A method of processing data associated with fluorescent emissions from a microfluidic device. The method includes performing an auto-focus process associated with a first image of the microfluidic device and performing an auto-exposure process associated with the first image of the microfluidic device. The method also includes capturing a plurality of images of the microfluidic device. The plurality of images are associated with a plurality of thermal cycles. The method further includes performing image analysis of the plurality of captured images to determine a series of optical intensities and performing data analysis of the series of optical intensities to provide a series of change in threshold values.
Abstract translation: 处理与微流体装置的荧光发射有关的数据的方法。 该方法包括执行与微流体装置的第一图像相关联的自动聚焦处理，并且执行与微流体装置的第一图像相关联的自动曝光处理。 该方法还包括捕获微流体装置的多个图像。 多个图像与多个热循环相关联。 该方法还包括执行多个拍摄图像的图像分析以确定一系列光强度并执行该系列光强度的数据分析以提供一系列阈值变化。
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公开(公告)号：US08721968B2
公开(公告)日：2014-05-13
申请号：US14031972
申请日：2013-09-19
Applicant: Fluidigm Corporation
Inventor： Marc A. Unger , Geoffrey Richard Facer , Barry Clerkson , Christopher G. Cesar , Neil Switz
CPC classification number: C12Q1/686 , B01J2219/00576 , B01J2219/00704 , B01L3/5027 , B01L7/52 , G01N21/64 , G01N21/6452 , G01N21/6456 , G01N21/6486 , G01N21/75 , G01N21/8483 , G01N27/44721 , G01N2021/6421 , G01N2201/061 , G02B21/16 , G02B21/36
Abstract: An apparatus for imaging one or more selected fluorescence indications from a microfluidic device. The apparatus includes an imaging path coupled to least one chamber in at least one microfluidic device. The imaging path provides for transmission of one or more fluorescent emission signals derived from one or more samples in the at least one chamber of the at least one microfluidic device. The chamber has a chamber size, the chamber size being characterized by an actual spatial dimension normal to the imaging path. The apparatus also includes an optical lens system coupled to the imaging path. The optical lens system is adapted to transmit the one or more fluorescent signals associated with the chamber.
Abstract translation: 一种用于从微流体装置成像一个或多个选定的荧光指示的装置。 该装置包括耦合到至少一个微流体装置中的至少一个室的成像路径。 成像路径提供从至少一个微流体装置的至少一个室中的一个或多个样品衍生的一个或多个荧光发射信号的传输。 腔室具有腔室尺寸，腔室尺寸的特征在于与成像路径垂直的实际空间尺寸。 该装置还包括耦合到成像路径的光学透镜系统。 光学透镜系统适于透射与腔室相关联的一个或多个荧光信号。
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Patent Agency Ranking
公开(公告)号：US20140045729A1
公开(公告)日：2014-02-13
申请号：US13863973
申请日：2013-04-16
Applicant: Fluidigm Corporation
Inventor： Robert C. Jones , Jing Wang , Andrew May , David Cohen
IPC: B01L3/00
CPC classification number: B01J19/00 , B01F5/0085 , B01F5/0602 , B01F13/0069 , B01J2219/00283 , B01J2219/00286 , B01J2219/00315 , B01J2219/00317 , B01J2219/00351 , B01J2219/00355 , B01J2219/00389 , B01J2219/00418 , B01L3/502715 , B01L3/502738 , B01L3/5085 , B01L7/52 , B01L2200/16 , B01L2300/0654 , B01L2300/0829 , B01L2300/123 , B01L2300/1805 , B01L2400/0481 , B01L2400/0605 , B01L2400/0655
Abstract: An SBS-formatted microfluidic device where the geometry of the plate defines an array of interrogation areas, and where each interrogation area encompasses at least one reaction site.
Abstract translation: SBS格式的微流体装置，其中板的几何形状限定了询问区域阵列，并且其中每个询问区域包含至少一个反应位点。
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公开(公告)号：US20140011204A1
公开(公告)日：2014-01-09
申请号：US13937340
申请日：2013-07-09
Inventor： Marc A. Unger , Geoffrey Richard Facer , Barry Clerkson , Christopher G. Cesar , Neil Switz
IPC: G01N21/64
CPC classification number: C12Q1/686 , B01J2219/00576 , B01J2219/00704 , B01L3/5027 , B01L7/52 , G01N21/64 , G01N21/6452 , G01N21/6456 , G01N21/6486 , G01N21/75 , G01N21/8483 , G01N27/44721 , G01N2021/6421 , G01N2201/061 , G02B21/16 , G02B21/36
Abstract: An apparatus for imaging one or more selected fluorescence indications from a microfluidic device. The apparatus includes an imaging path coupled to least one chamber in at least one microfluidic device. The imaging path provides for transmission of one or more fluorescent emission signals derived from one or more samples in the at least one chamber of the at least one microfluidic device. The chamber has a chamber size, the chamber size being characterized by an actual spatial dimension normal to the imaging path. The apparatus also includes an optical lens system coupled to the imaging path. The optical lens system is adapted to transmit the one or more fluorescent signals associated with the chamber.
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公开(公告)号：US20130255799A1
公开(公告)日：2013-10-03
申请号：US13773518
申请日：2013-02-21
Applicant: FLUIDIGM CORPORATION
Inventor： Naga Gopi Devaraju , Marc A. Unger
IPC: F17D3/00
CPC classification number: B01L3/502738 , B01L3/502707 , B01L3/502715 , B01L2200/14 , B01L2300/023 , B01L2300/0816 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/123 , B01L2300/14 , B01L2400/0605 , B01L2400/0633 , B01L2400/0655 , F17D3/00 , G01N35/00871 , G01N2035/00247 , Y10T137/7762
Abstract: A microfluidic device includes an input source characterized by a source pressure and an input channel in fluid communication with the input source. The microfluidic device also includes an output channel and a valve having an open state and a closed state. The valve is disposed between the input channel and the output channel and is characterized by a static pressure. The microfluidic device further includes a control channel coupled to the valve and characterized by a control pressure. In the closed state, the control pressure is greater than atmospheric pressure.
Abstract translation: 微流体装置包括以源压力为特征的输入源和与输入源流体连通的输入通道。 微流体装置还包括输出通道和具有打开状态和关闭状态的阀。 阀设置在输入通道和输出通道之间，其特征在于静压。 微流体装置还包括连接到阀的控制通道，其特征在于控制压力。 在关闭状态下，控制压力大于大气压。
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公开(公告)号：US20130252234A1
公开(公告)日：2013-09-26
申请号：US13894296
申请日：2013-05-14
Applicant: Fluidigm Corporation
Inventor： Hany Nassef , Greoffrey Facer , Marc Unger
IPC: G01N27/60
CPC classification number: C03C25/68 , B01L3/502707 , B01L2300/0645 , B01L2300/0816 , B01L2300/123 , B01L2400/0481 , B01L2400/0655 , G01N15/1031 , G01N15/12 , G01N27/00 , G01N27/60 , G01N33/48707 , G01N33/48721 , G01N2015/1087 , Y10T436/2575
Abstract: The presence of a detectable entity within a detection volume of a microfabricated elastomeric structure is sensed through a change in the electrical or magnetic environment of the detection volume. In embodiments utilizing electronic detection, an electric field is applied to the detection volume and a change in impedance, current, or combined impedance and current due to the presence of the detectable entity is measured. In embodiments utilizing magnetic detection, the magnetic properties of a magnetized detected entity alter the magnetic field of the detection volume. This changed magnetic field induces a current which can reveal the detectable entity. The change in resistance of a magnetoresistive element may also reveal the passage of a magnetized detectable entity.
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公开(公告)号：US20040072278A1
公开(公告)日：2004-04-15
申请号：US10405953
申请日：2003-04-01
Applicant: Fluidigm Corporation
Inventor： Hou-Pu Chou , Antoine Daridon , Kevin Farrell , Brian Fowler , Yish-Hann Liau , Ian D. Manger , Hany Ramez Nassef , William Throndset
IPC: G01N033/00 , C12M001/34 , C12Q001/02
CPC classification number: G01N35/00 , B01L3/502746 , B01L3/502753 , B01L3/502761 , B01L2200/0636 , B01L2200/0647 , B01L2200/0652 , B01L2200/0668 , B01L2200/10 , B01L2200/12 , B01L2300/0645 , B01L2300/0681 , B01L2300/0861 , B01L2300/0867 , B01L2300/087 , B01L2300/0887 , B01L2300/123 , B01L2400/0409 , B01L2400/0481 , B01L2400/0655 , G01N15/1456 , G01N15/1468 , G01N15/1484 , G01N2015/0288 , G01N2015/1075 , G01N2015/149 , G01N2015/1493 , Y10T436/11 , Y10T436/2575
Abstract: The invention provides systems, including apparatus, methods, and kits, for the microfluidic manipulation and/or detection of particles, such as cells and/or beads. The invention provides systems, including apparatus, methods, and kits, for the microfluidic manipulation and/or analysis of particles, such as cells, viruses, organelles, beads, and/or vesicles. The invention also provides microfluidic mechanisms for carrying out these manipulations and analyses. These mechanisms may enable controlled input, movement/positioning, retention/localization, treatment, measurement, release, and/or output of particles. Furthermore, these mechanisms may be combined in any suitable order and/or employed for any suitable number of times within a system. Accordingly, these combinations may allow particles to be sorted, cultured, mixed, treated, and/or assayed, among others, as single particles, mixed groups of particles, arrays of particles, heterogeneous particle sets, and/or homogeneous particle sets, among others, in series and/or in parallel. In addition, these combinations may enable microfluidic systems to be reused. Furthermore, these combinations may allow the response of particles to treatment to be measured on a shorter time scale than was previously possible. Therefore, systems of the invention may allow a broad range of cell and particle assays, such as drug screens, cell characterizations, research studies, and/or clinical analyses, among others, to be scaled down to microfluidic size. Such scaled-down assays may use less sample and reagent, may be less labor intensive, and/or may be more informative than comparable macrofluidic assays.
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