公开(公告)号：US09535033B2

公开(公告)日：2017-01-03

申请号：US14421809

申请日：2013-08-02

Applicant: Quantum Biosystems Inc.

Inventor： Tomoji Kawai ,  Takahito Ohshiro ,  Masateru Taniguchi

IPC: G01N27/403 ,  G01N27/453 ,  G01N27/447 ,  G01N27/327 ,  G01N33/487 ,  G01N15/10

CPC classification number: G01N27/447 ,  G01N15/1031 ,  G01N27/3275 ,  G01N27/4473 ,  G01N33/48721 ,  G01N2015/1006

Abstract: The analysis method allows analysis of samples with high sensitivity, irrespective of interelectrode distance. The method includes: a step of applying a voltage between a first electrode pair such that an electric field is formed in a direction intersecting a migration direction of a sample; a step of placing a solution, including an electrochemically active molecule that produces a redox reaction at the electrode pair, between the first electrode pair; a step of causing the sample to migrate; and a step of measuring an amount of change in current flow between the first electrode pair.

Abstract translation: 分析方法允许分析具有高灵敏度的样品，而不考虑电极间距离。 该方法包括：在与样品的迁移方向相交的方向形成电场的第一电极对之间施加电压的步骤; 在第一电极对之间放置包括在电极对产生氧化还原反应的电化学活性分子的溶液的步骤; 使样品迁移的步骤; 以及测量第一电极对之间的电流变化量的步骤。

公开(公告)号：US20160320364A1

公开(公告)日：2016-11-03

申请号：US15098147

申请日：2016-04-13

Applicant: Quantum Biosystems Inc.

Inventor： Shuji Ikeda ,  Mark Oldham ,  Eric S. Nordman

IPC: G01N33/487 ,  C23C16/455 ,  G01N27/447

CPC classification number: G01N33/48721 ,  C23C16/45525 ,  G01N27/44791

Abstract: A system for detecting a biomolecule comprises a nano-gap electrode device including a first electrode and a second electrode adjacent to the first electrode. The first electrode can be separated from the second electrode by a nano-gap that is dimensioned to permit the biomolecule to flow through the nano-gap. The nano-gap can have at least a first gap region and a second gap region. The second gap region can be oriented at an angle that is greater than zero degrees with respect to a plane having the first gap region. The system can further include an electrical circuit coupled to the nano-gap electrode device. The electrical circuit can receive electrical signals from the first electrode and the second electrode upon the flow of the biomolecule through the nano-gap.

Abstract translation: 用于检测生物分子的系统包括纳米间隙电极装置，其包括与第一电极相邻的第一电极和第二电极。 第一电极可以通过纳米间隙与第二电极分离，其尺寸被设计成允许生物分子流过纳米间隙。 纳米间隙可以具有至少第一间隙区域和第二间隙区域。 第二间隙区域可相对于具有第一间隙区域的平面以大于零度的角度定向。 该系统还可以包括耦合到纳米间隙电极器件的电路。 当生物分子流过纳米间隙时，电路可以接收来自第一电极和第二电极的电信号。

公开(公告)号：US20160245790A1

公开(公告)日：2016-08-25

申请号：US15048889

申请日：2016-02-19

Applicant: Osaka University ,  Quantum Biosystems Inc.

Inventor： Tomoji Kawai ,  Masayuki Furuhashi ,  Masateru Kawaguchi ,  Mark Oldham ,  Eric Nordman

IPC: G01N33/487 ,  G01N27/26 ,  C12Q1/68 ,  B01L3/00 ,  B01L7/00

CPC classification number: G01N33/48721 ,  B01L3/502715 ,  B01L7/52 ,  B01L7/525 ,  B01L2200/0663 ,  B01L2200/10 ,  B01L2300/04 ,  B01L2300/0645 ,  B01L2300/0663 ,  B01L2300/0816 ,  B01L2300/0896 ,  B01L2300/12 ,  B01L2300/1827 ,  B01L2400/086 ,  C12Q1/6813 ,  C12Q1/6869 ,  G01N27/26 ,  G01N27/3278 ,  C12Q2527/107 ,  C12Q2527/137 ,  C12Q2563/159 ,  C12Q2565/629 ,  C12Q2565/631

Abstract: The present disclosure provides methods and systems that can reduce the amount of sample necessary to detect or identify, or both detect and identify, a biomolecule, and increase the rate of denaturing of the biomolecule. A device for thermally denaturing a biomolecule may include: a substrate having low thermal conductivity; a heater disposed adjacent to the substrate; a temperature sensor disposed adjacent to the substrate; a semiconductor oxide film disposed adjacent to the substrate, a nanochannel formed in a region of the semiconductor oxide film, and a cover over the nanochannel.

Abstract translation: 本公开提供了可以减少检测或识别或既检测和鉴定生物分子又增加生物分子的变性速率所需的样品量的方法和系统。 用于热变性生物分子的装置可以包括：具有低导热性的基底; 靠近所述基板设置的加热器; 设置在所述基板附近的温度传感器; 邻近衬底设置的半导体氧化膜，形成在半导体氧化膜的区域中的纳米通道以及纳米通道上的覆盖层。

公开(公告)号：US20190310240A1

公开(公告)日：2019-10-10

申请号：US16178924

申请日：2018-11-02

Applicant: Quantum Biosystems Inc.

Inventor： Shuji Ikeda ,  Mark Oldham ,  Eric S. Nordman

IPC: G01N33/487 ,  G01N27/327 ,  C23C16/455 ,  G01N27/447

Abstract: A system for detecting a biomolecule comprises a nano-gap electrode device including a first electrode and a second electrode adjacent to the first electrode. The first electrode can be separated from the second electrode by a nano-gap that is dimensioned to permit the biomolecule to flow through the nano-gap. The nano-gap can have at least a first gap region and a second gap region. The second gap region can be oriented at an angle that is greater than zero degrees with respect to a plane having the first gap region. The system can further include an electrical circuit coupled to the nano-gap electrode device. The electrical circuit can receive electrical signals from the first electrode and the second electrode upon the flow of the biomolecule through the nano-gap.

公开(公告)号：US10438811B1

公开(公告)日：2019-10-08

申请号：US14687856

申请日：2015-04-15

Applicant: Quantum Biosystems Inc.

Inventor： Shuji Ikeda

IPC: H01L21/311 ,  H01L21/285 ,  C12Q1/6869

Abstract: Methods for forming electrodes for use in nano-gap electrodes are provided. Such methods can be used to form electrodes for use in devices that can be used to sense or detect biomolecules, such as in biomolecule sequence applications.

公开(公告)号：US10202644B2

公开(公告)日：2019-02-12

申请号：US14883494

申请日：2015-10-14

Applicant: Quantum Biosystems Inc.

Inventor： Masateru Taniguchi ,  Makusu Tsutsui ,  Kazumichi Yokota ,  Tomoji Kawai

IPC: C12Q1/6874 ,  G01N27/327 ,  C12Q1/6869

Abstract: The present invention provides technology that uses current measurements to identify nucleotides and determine a nucleotide sequence in polynucleotides. The present invention calculates a modal value of a tunnel current that arises when a nucleotide or polynucleotide for analysis passes through between electrodes, and then employs the calculated modal value. The present invention accordingly enables direct rapid implementation to identify nucleotides and to determine a nucleotide sequence in a polynucleotide without marking.

公开(公告)号：US20180023132A1

公开(公告)日：2018-01-25

申请号：US15448317

申请日：2017-03-02

Applicant: Quantum Biosystems Inc.

Inventor： Tomoji Kawai ,  Masateru Taniguchi ,  Takahito Ohshiro ,  Mark Oldham ,  Eric Nordman

IPC: C12Q1/68 ,  G06F19/22 ,  G01N27/04

Abstract: Devices, systems and methods for sequencing protein samples are provided. In some examples, currents generated when a monomer passes through between electrodes of a nanogap electrode pair are measured for each of several different distances, so that monomers are identified when compared to a reference physical quantity of a known monomer, which may be obtained from a current measured with a similar inter-electrode distance(s) at which each of plural kinds of monomers are identifiable and ordered with predetermined accuracy and based on a detected physical quantity obtained from a tunneling current, which may be further normalized by the use of one or more reference substances.

公开(公告)号：US09644236B2

公开(公告)日：2017-05-09

申请号：US15061871

申请日：2016-03-04

Applicant: Quantum Biosystems Inc.

Inventor： Tomoji Kawai ,  Masateru Taniguchi ,  Takahito Ohshiro ,  Mark Oldham ,  Eric Nordman

IPC: C12Q1/68 ,  G01N27/64 ,  G06F19/22 ,  G01N27/04

CPC classification number: C12Q1/6869 ,  G01N27/04 ,  G01N33/48721 ,  G06F19/22 ,  C12Q2563/116 ,  C12Q2565/631

Abstract: Devices, systems and methods for sequencing protein samples are provided. In some examples, currents generated when a monomer passes through between electrodes of a nanogap electrode pair are measured for each of several different distances, so that monomers are identified when compared to a reference physical quantity of a known monomer, which may be obtained from a current measured with a similar inter-electrode distance(s) at which each of plural kinds of monomers are identifiable and ordered with predetermined accuracy and based on a detected physical quantity obtained from a tunneling current, which may be further normalized by the use of one or more reference substances.

公开(公告)号：US20160245789A1

公开(公告)日：2016-08-25

申请号：US15048810

申请日：2016-02-19

Applicant: Quantum Biosystems Inc.

Inventor： Shuji Ikeda ,  Mark Oldham ,  Eric Nordman

IPC: G01N33/487 ,  C23C16/34 ,  C12Q1/68 ,  C23C16/56 ,  C23C14/06 ,  C23C14/58 ,  G01N27/447 ,  C23C16/06

CPC classification number: G01N33/48721 ,  B82Y40/00 ,  C12Q1/6869 ,  C23C14/0641 ,  C23C14/5886 ,  C23C16/06 ,  C23C16/345 ,  C23C16/56 ,  G01N27/3278 ,  G01N27/44704 ,  G01N27/44791

Abstract: The present disclosure provides methods for forming a nano-gap electrode. In some cases, a nano-gap having a width adjusted by a film thickness of a sidewall may be formed between a first electrode-forming part and a second electrode-forming part using sidewall which has contact with first electrode-forming part as a mask. Surfaces of the first electrode-forming part, the sidewall and the second electrode-forming part may then be exposed. The sidewall may then be removed to form a nano-gap between the first electrode-forming part and the second electrode-forming part.

Abstract translation: 本公开提供了形成纳米间隙电极的方法。 在一些情况下，可以使用与第一电极形成部分接触的侧壁作为掩模，在第一电极形成部分和第二电极形成部分之间形成具有由侧壁的膜厚度调节的宽度的纳米间隙 。 然后可以露出第一电极形成部分，侧壁和第二电极形成部分的表面。 然后可以去除侧壁以在第一电极形成部分和第二电极形成部分之间形成纳米间隙。

公开(公告)号：US20210238672A1

公开(公告)日：2021-08-05

申请号：US17238090

申请日：2021-04-22

Applicant: Quantum Biosystems Inc.

Inventor： Eric S. NORDMAN ,  Valentin DIMITROV ,  Gaurav GOYAL ,  Jessica KILLIAN ,  Kazusuke MIHARA

IPC: C12Q1/6869 ,  C12Q1/00 ,  C12Q1/48

Abstract: The present disclosure provides methods and systems for detecting or analyzing a biomolecule. The methods and systems may comprise the use of a plurality of electrodes and a solution which may comprise a variety of reagents. In an example, the solution may comprise one or more redox-active molecules or compounds which may undergo or facilitate a redox cycling process. The redox cycling process may generate an electric signal that may be measured by one or more of the plurality of electrodes. An introduction of a nucleotide having a redox cycling current modifying particle coupled thereto to the solution may result in a change in the electric signal. The change in the electric signal may be used to identify the biomolecule or a portion thereof.