公开(公告)号：US20240253044A1

公开(公告)日：2024-08-01

申请号：US18376276

申请日：2023-10-03

Applicant: 10x Genomics, Inc.

Inventor： Rajiv BHARADWAJ ,  Anthony MAKAREWICZ ,  Bill Kengli LIN

IPC: B01L3/00 ,  B01F23/41 ,  B01F25/314 ,  B01F31/80 ,  B01F33/3011 ,  B01F33/302 ,  B01L3/02 ,  C12Q1/6806 ,  C12Q1/686 ,  C40B40/06 ,  G01N33/543 ,  G01N33/58

CPC classification number: B01L3/502784 ,  B01F23/41 ,  B01F23/4105 ,  B01F25/3141 ,  B01F25/31422 ,  B01F25/31423 ,  B01F31/84 ,  B01F33/3011 ,  B01F33/3021 ,  B01L3/0241 ,  B01L3/50273 ,  B01L3/502761 ,  C12Q1/6806 ,  G01N33/54313 ,  G01N33/58 ,  B01L2200/0647 ,  B01L2200/0652 ,  B01L2200/0673 ,  B01L2300/0851 ,  B01L2300/161 ,  B01L2400/086 ,  C12N2533/50 ,  C12Q1/686 ,  C12Q2563/185 ,  C40B40/06

Abstract: Devices, systems, and their methods of use, for generating droplets are provided. One or more geometric parameters of a microfluidic channel can be selected to generate droplets of a desired and predictable droplet size.

公开(公告)号：US20230173491A1

公开(公告)日：2023-06-08

申请号：US18161643

申请日：2023-01-30

Applicant: 10x Genomics, Inc.

Inventor： Rajiv BHARADWAJ ,  Anthony MAKAREWICZ ,  Bill Kengli LIN

IPC: B01L3/00 ,  G01N33/543 ,  B01F23/41 ,  B01F25/314 ,  B01F31/80 ,  B01F33/3011 ,  B01F33/302 ,  B01L3/02 ,  C12Q1/6806 ,  G01N33/58

CPC classification number: B01L3/502784 ,  B01L3/502761 ,  G01N33/54313 ,  B01F23/41 ,  B01F23/4105 ,  B01F25/3141 ,  B01F25/31422 ,  B01F25/31423 ,  B01F31/84 ,  B01F33/3011 ,  B01F33/3021 ,  B01L3/0241 ,  B01L3/50273 ,  C12Q1/6806 ,  G01N33/58 ,  B01L2300/0851 ,  B01L2300/161 ,  B01L2200/0652 ,  B01L2400/086 ,  B01L2200/0673 ,  B01L2200/0647 ,  C12Q1/686

Abstract: Devices, systems, and their methods of use, for generating droplets are provided. One or more geometric parameters of a microfluidic channel can be selected to generate droplets of a desired and predictable droplet size.

公开(公告)号：US20220410162A1

公开(公告)日：2022-12-29

申请号：US17851416

申请日：2022-06-28

Applicant: 10x Genomics, Inc.

Inventor： Rajiv BHARADWAJ ,  Michael SCHNALL-LEVIN ,  Anthony MAKAREWICZ ,  Steven SHORT

IPC: B01L3/00 ,  G01N15/10 ,  G01N15/14

Abstract: Methods and systems for sorting particles are provided. Methods and systems for sorting cell beads are provided. In some cases, cell beads may be sorted from particles unoccupied with cell derivatives. In some cases, singularly occupied cell beads may be sorted from unoccupied particles and multiply occupied cell beads.

公开(公告)号：US20220280933A1

公开(公告)日：2022-09-08

申请号：US17751267

申请日：2022-05-23

Applicant: 10x Genomics, Inc.

Inventor： Rajiv BHARADWAJ ,  Hanyoup KIM ,  Bill Kengli LIN ,  Marissa PENNELL ,  Alireza SALMANZADEH ,  Martin SAUZADE ,  Tobias Daniel WHEELER

IPC: B01L3/02 ,  B01L3/00

Abstract: Devices, systems, and their methods of use, for generating droplets are provided. The devices, systems, and methods may include transporting a first liquid through an outlet of a channel and causing relative motion of the outlet and an interface of a second liquid to produce droplets of the first liquid in the second liquid. The devices, systems, and methods may also include illuminating a portion of the liquid as the liquid exits from an outlet. The invention also provides methods, devices, and systems for changing the size of a droplet and for eliminating a droplet from a plurality of droplets.

公开(公告)号：US20210362157A1

公开(公告)日：2021-11-25

申请号：US17397146

申请日：2021-08-09

Applicant: 10x Genomics, Inc.

Inventor： Rajiv BHARADWAJ ,  Kevin NESS ,  Tobias Daniel WHEELER

IPC: B01L3/00 ,  G01N1/28 ,  G01N33/487

Abstract: Microfluidic channels networks and systems are provided. One network includes a first fluid channel having a first depth dimension; at least a second channel intersecting the first channel at a first intersection; at least a third channel in fluid communication with the first intersection, at least one of the first intersection and the third channel having a depth dimension that is greater than the first depth dimension. Also provided is a flow control system for directing fluids in the network. Systems are additionally provided for flowing disrupted particles into a droplet formation junction, whereby a portion of the disrupted particles or the contents thereof are encapsulated into one or more droplets. Further provided is a method for controlling filling of a microfluidic network by controlling passive valving microfluidic channel network features.

公开(公告)号：US20200290049A1

公开(公告)日：2020-09-17

申请号：US16887024

申请日：2020-05-29

Applicant: 10X Genomics, Inc.

Inventor： Rajiv BHARADWAJ ,  Tobias Daniel WHEELER ,  Kevin NESS ,  Benjamin HINDSON

IPC: B01L3/00 ,  B01F13/00 ,  B01J2/06 ,  C12Q1/6844 ,  B01F3/08

Abstract: A microfluidic emulsion droplet generation system and methods of use thereof are provided. The system may include a microfluidic substrate having a flow path configured and arranged for emulsion droplet generation, at least one textured surface in the flow path configured and arranged for inducing surface-mediated coalescence of emulsion droplets; and at least one channel junction in the flow path for emulsion droplet formation.

公开(公告)号：US20200206742A1

公开(公告)日：2020-07-02

申请号：US16812866

申请日：2020-03-09

Applicant: 10X Genomics, Inc.

Inventor： Rajiv BHARADWAJ ,  Anthony MAKAREWICZ ,  Bill Kengli LIN

IPC: B01L3/00 ,  B01F5/04 ,  G01N33/543 ,  B01F3/08 ,  B01F13/00 ,  B01F11/02 ,  B01L3/02 ,  C12Q1/6806 ,  G01N33/58

Abstract: Devices, systems, and their methods of use, for generating droplets are provided. One or more geometric parameters of a microfluidic channel can be selected to generate droplets of a desired and predictable droplet size.

公开(公告)号：US20240218437A1

公开(公告)日：2024-07-04

申请号：US18542038

申请日：2023-12-15

Applicant: 10x Genomics, Inc.

Inventor： Zahra Kamila BELHOCINE ,  Zachary W. BENT ,  Rajiv BHARADWAJ ,  Alexander GAGNON ,  Qiang GONG ,  Ashley HAYES ,  Benjamin HINDSON ,  Yuwei LI ,  Benjamin PRUITT ,  Daniel P. RIORDAN ,  Hiroshi SASAKI ,  Weiyi TANG

IPC: C12Q1/6844 ,  C12Q1/6816

CPC classification number: C12Q1/6846 ,  C12Q1/6816

Abstract: The present disclosure relates in some aspects to methods and compositions for assessing system performance for in situ analyte detection. In some aspects, performance of an individual instrument can be assessed, or the performance of two or more instruments can be assessed and optionally compared. In some aspects, disclosed herein is a method comprising using rolling circle amplification products (RCPs) deposited on a cell-free and tissue-free quality control (QC) slide to assess performance of instrument workflow, where an instrument is used to decode signals associated with the RCPs on the QC slide. Quality metrics associated with the decoding (e.g., a percentage of RCPs successfully decoded to genes) can be used to qualify a system comprising the instrument and reagents for in situ analyte detection in cells or tissue samples, e.g., using in situ probe hybridization or in situ sequencing performed on the instrument.

公开(公告)号：US20230271187A1

公开(公告)日：2023-08-31

申请号：US18143272

申请日：2023-05-04

Applicant: 10x Genomics, Inc.

Inventor： Rajiv BHARADWAJ ,  Michael SCHNALL-LEVIN ,  Anthony MAKAREWICZ ,  Steven SHORT

IPC: B01L3/00 ,  G01N15/10 ,  G01N15/14

CPC classification number: B01L3/502761 ,  B01L3/502715 ,  B01L3/502784 ,  G01N15/1031 ,  G01N15/1056 ,  G01N15/1484 ,  B01L2200/0652 ,  B01L2200/0673 ,  B01L2300/0867 ,  B01L2400/0415 ,  B01L2400/043 ,  G01N2015/1081 ,  G01N2015/149

Abstract: Methods and systems for sorting particles are provided. Methods and systems for sorting cell beads are provided. In some cases, cell beads may be sorted from particles unoccupied with cell derivatives. In some cases, singularly occupied cell beads may be sorted from unoccupied particles and multiply occupied cell beads.

公开(公告)号：US20230167489A1

公开(公告)日：2023-06-01

申请号：US18070887

申请日：2022-11-29

Applicant: 10x Genomics, Inc.

Inventor： Yiran ZHANG ,  Siyuan XING ,  Rajiv BHARADWAJ

IPC: C12Q1/6841 ,  C12Q1/6837

CPC classification number: C12Q1/6841 ,  C12Q1/6837

Abstract: The present invention features devices and methods for supplying a biological sample with a fluid. In various embodiments, a device includes a first layer and a second layer. The first layer includes a window having a thickness of about 1 μm to about 1000 μm. The first and second layers reversibly seal to form a flow path having an inlet and an outlet and bounded in part by the window.