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    Method of refolding human activin A
    11.
    发明授权
    Method of refolding human activin A 有权
    人激活素A重折叠的方法

    公开(公告)号：US6084076A

    公开(公告)日：2000-07-04

    申请号：US91265

    申请日：1999-02-08

    Applicant: Daisuke Ejima ,  Kunio Ono ,  Michiro Sasaki ,  Yuzuru Eto ,  Shigekatsu Tsuchiya

    Inventor： Daisuke Ejima ,  Kunio Ono ,  Michiro Sasaki ,  Yuzuru Eto ,  Shigekatsu Tsuchiya

    IPC: C07K14/575 ,  C12P21/04 ,  C07K14/475 ,  A61K38/18 ,  C07K1/06 ,  C12N15/09

    CPC classification number: C07K14/575

    Abstract: A method for industrially producing human activin A which comprises refolding a modified human activin A produced by a microorganism into natural-form human activin A having a biological activity. This method comprises the steps of: (a) solubilizing the modified human activin A with a modifier and then protecting the thiol groups with glutathione and/or sodium sulfite; and (b) dialyzing the protected modified human activin A with a refolding buffer, containing taurodexoycholic acid or its salts and then adding a thiol compound thereto conduct a disulfide bond interchange reaction.

    Abstract translation: PCT No.PCT / JP96 / 03700 Sec。 371日期1999年2月8日 102（e）1999年2月8日PCT 1996年12月19日PCT PCT。 公开号WO97 / 23638 PCT 日期1997年7月3日工业生产人激活素A的方法，其包括将由微生物产生的经修饰的人激活素A重折叠成具有生物活性的天然形式的人激活素A. 该方法包括以下步骤：（a）用修饰剂溶解修饰的人激活素A，然后用谷胱甘肽和/或亚硫酸钠保护硫醇基团; 和（b）用含有牛磺去氧胆酸或其盐的重折叠缓冲液透析受保护的修饰的人激活素A，然后加入硫醇化合物进行二硫键交换反应。

    METHOD FOR IMPROVING RECOVERY YIELD IN PROTEIN PURIFICATION WITH GEL FILTRATION CHROMATOGRAPHY
    15.
    发明申请
    METHOD FOR IMPROVING RECOVERY YIELD IN PROTEIN PURIFICATION WITH GEL FILTRATION CHROMATOGRAPHY 有权
    改进凝胶过滤色谱法提纯蛋白质回收率的方法

    公开(公告)号：US20060199948A1

    公开(公告)日：2006-09-07

    申请号：US11276509

    申请日：2006-03-03

    Applicant: Daisuke Ejima ,  Ryosuke Yumioka ,  Tsutomu Arakawa

    Inventor： Daisuke Ejima ,  Ryosuke Yumioka ,  Tsutomu Arakawa

    IPC: C07K16/00 ,  C07K1/10

    CPC classification number: C07K1/20 ,  C07K1/14 ,  C07K14/50 ,  C07K14/5412 ,  C07K14/55 ,  C07K14/575 ,  C07K14/755 ,  C07K16/36

    Abstract: A method to more quantitatively recover a peak of a protein and associated aggregates, hydrophobic proteins, or hydrophobic peptides which are typically difficult to recover and detect as a peak, using a commercially available gel filtration chromatography column and a mobile phase is described. More specifically, a compound, such as arginine, which may be added to the mobile phase, thereby eliminating the unnecessary interaction that occurs between the proteins, peptides, or aggregates and the column stationary phase is described.

    Abstract translation: 描述了使用市售的凝胶过滤色谱柱和流动相来更定量地回收通常难以回收和检测作为峰的相关聚集体，疏水性蛋白质或疏水性肽的峰值的方法。 更具体地，描述了可以添加到流动相中的化合物，例如精氨酸，从而消除了在蛋白质，肽或聚集体和柱固定相之间发生的不必要的相互作用。

    Protein refolding method
    17.
    发明授权
    Protein refolding method 有权
    蛋白重折叠法

    公开(公告)号：US08975383B2

    公开(公告)日：2015-03-10

    申请号：US12941272

    申请日：2010-11-08

    Applicant: Ryosuke Yumioka ,  Daisuke Ejima

    Inventor： Ryosuke Yumioka ,  Daisuke Ejima

    IPC: C07K1/113 ,  C07K1/14 ,  C07K14/54 ,  C07K14/475 ,  C12N9/10 ,  C07K16/18 ,  C07K16/44

    CPC classification number: C07K14/5412 ,  C07K1/1136 ,  C07K1/145 ,  C07K14/475 ,  C07K16/18 ,  C07K16/44 ,  C07K2317/569 ,  C07K2317/64 ,  C12N9/104 ,  C12N9/1044

    Abstract: The present invention provides a method for producing a protein which has a restored native higher-order structure by bringing a protein which has lost its native higher-order structure into contact at pH 6.5 to 9.0 with a 1 to 3% aqueous solution of a specific surfactant, such as lauroylglutamic acid to obtain a solubilized solution of the protein; and then adding the solubilized solution to a buffer with pH 6.5 to 9.0 containing arginine or an arginine derivative at a concentration of 0.1 to 1.2 M to lower the concentration of the specific surfactant, such as lauroylglutamic acid, in the obtained mixture solution down to 0.02 to 0.275%. According to the present invention, it is possible to easily restore the native higher-order structure of a protein while smoothly removing the surfactant from the protein.

    Abstract translation: 本发明提供一种生产蛋白质的方法，该蛋白质具有恢复的天然高级结构，通过使具有1至3％的特异性的1％至3％的水溶液使具有失去其天然高级结构的蛋白质在pH 6.5至9.0下接触 表面活性剂如月桂酰谷氨酸以获得蛋白质的溶解溶液; 然后将溶解的溶液加入到含有浓度为0.1至1.2M的精氨酸或精氨酸衍生物的pH 6.5至9.0的缓冲液中，以将得到的混合溶液中的特定表面活性剂如月桂酰谷氨酸的浓度降低至0.02 至0.275％。 根据本发明，可以容易地恢复蛋白质的天然高级结构，同时平滑地从蛋白质中除去表面活性剂。

    FOLDING PORTABLE DEVICE, HINGE MECHANISM FOR THE DEVICE, AND METHOD OF OPENING THE DEVICE
    19.
    发明申请
    FOLDING PORTABLE DEVICE, HINGE MECHANISM FOR THE DEVICE, AND METHOD OF OPENING THE DEVICE 有权
    折叠便携式装置，装置的铰链机构以及开启装置的方法

    公开(公告)号：US20120013231A1

    公开(公告)日：2012-01-19

    申请号：US13057848

    申请日：2009-09-15

    Applicant: Daisuke Ejima

    Inventor： Daisuke Ejima

    IPC: A47B96/00 ,  E05F1/12 ,  E05D5/02

    CPC classification number: H04M1/0216 ,  H04M1/0227 ,  Y10T16/538 ,  Y10T16/53832 ,  Y10T16/53888 ,  Y10T16/558

    Abstract: A folding portable device in which a first case and a second case are foldably connected and which can be opened by a simple operation even when a thickness of the case is reduced includes support means for supporting the first case with respect to the second case in a relatively displaceable manner within a specified range and opening assistance means of which an open lock is unlocked when the first case is displaced more than a predetermined amount.

    Abstract translation: 一种折叠便携式装置，其中第一壳体和第二壳体可折叠地连接，并且即使当壳体的厚度减小时也可以通过简单的操作来打开，包括用于相对于第二壳体在第一壳体中支撑第一壳体的支撑装置 在规定范围内可相对移动的方式和打开辅助装置，当第一壳体移动超过预定量时，打开辅助装置打开锁定。

    PORTABLE INFORMATION PROCESSING TERMINAL
    20.
    发明申请
    PORTABLE INFORMATION PROCESSING TERMINAL 有权
    便携式信息处理终端

    公开(公告)号：US20110205694A1

    公开(公告)日：2011-08-25

    申请号：US12675936

    申请日：2008-10-06

    Applicant: Daisuke Ejima

    Inventor： Daisuke Ejima

    IPC: H05K5/02

    CPC classification number: H04M1/0237 ,  H04M1/0233

    Abstract: To reduce the thickness and improve the operability. It includes a first housing and a second housing placed on top of each other, and connecting means to connect the first and second housings, the connecting means being interposed between the first and second housings, wherein the connecting means engages with the first housing so as to be slidable in at least one direction with respect to the first housing, and engages with the second housing so as to be rotatable with respect to the second housing.

    Abstract translation: 减小厚度，提高可操作性。 它包括彼此顶部放置的第一壳体和第二壳体，以及用于连接第一和第二壳体的连接装置，连接装置设置在第一和第二壳体之间，其中连接装置与第一壳体接合，以便 能够相对于第一壳体在至少一个方向上滑动，并且与第二壳体接合以能够相对于第二壳体旋转。

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