公开(公告)号：US11680292B2

公开(公告)日：2023-06-20

申请号：US17840569

申请日：2022-06-14

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

Inventor： Jeffrey S. Fisher ,  Brian D. Mather ,  Maria Candelaria Rogert Bacigalupo ,  Justin Fullerton ,  Ludovic Vincent ,  Lewis J. Kraft ,  Sahngki Hong ,  Boyan Boyanov ,  M. Shane Bowen ,  Sang Park ,  Wayne N. George ,  Andrew A. Brown

IPC: C12Q1/6869 ,  C08F220/56 ,  C08G77/04 ,  G03F7/075 ,  G03F7/16 ,  G03F7/00 ,  G03F7/004

CPC classification number: C12Q1/6869 ,  C08F220/56 ,  C08G77/04 ,  G03F7/0757 ,  G03F7/165 ,  G03F7/0002 ,  G03F7/0046

Abstract: An example of a flow cell includes a substrate; a first primer set attached to a first region on the substrate, the first primer set including an un-cleavable first primer and a cleavable second primer; and a second primer set attached to a second region on the substrate, the second primer set including a cleavable first primer and an un-cleavable second primer.

公开(公告)号：US20230002822A1

公开(公告)日：2023-01-05

申请号：US17840569

申请日：2022-06-14

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

Inventor： Jeffrey S. Fisher ,  Brian D. Mather ,  Maria Candelaria Rogert Bacigalupo ,  Justin Fullerton ,  Ludovic Vincent ,  Lewis J. Kraft ,  Sahngki Hong ,  Boyan Boyanov ,  M. Shane Bowen ,  Sang Park ,  Wayne N. George ,  Andrew A. Brown

IPC: C12Q1/6869 ,  C08F220/56 ,  C08G77/04 ,  G03F7/075 ,  G03F7/16

Abstract: An example of a flow cell includes a substrate; a first primer set attached to a first region on the substrate, the first primer set including an un-cleavable first primer and a cleavable second primer; and a second primer set attached to a second region on the substrate, the second primer set including a cleavable first primer and an un-cleavable second primer.

公开(公告)号：US20220097048A1

公开(公告)日：2022-03-31

申请号：US17486549

申请日：2021-09-27

Applicant: ILLUMINA, INC.

Inventor： Brinda Kodira Cariappa ,  Jeffrey S. Fisher ,  Sahngki Hong ,  Lewis J. Kraft ,  Brian D. Mather ,  Vanessa Montaño-Machado

IPC: B01L3/00 ,  G03F7/00 ,  G03F7/038 ,  G03F7/20 ,  G03F7/32 ,  G03F7/16

Abstract: In one example, a flow cell includes a base support and a protrusion over the base support, where the protrusion is a different material than the base support. The flow cell further includes a first functionalized layer over a first portion of the protrusion, a second functionalized layer over a second portion of the protrusion, and first and second primer sets respectively attached to the first and second functionalized layers.

公开(公告)号：US11124829B2

公开(公告)日：2021-09-21

申请号：US16626452

申请日：2019-06-07

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

Inventor： Jeffrey S. Fisher ,  Brian D. Mather ,  Maria Candelaria Rogert Bacigalupo ,  Justin Fullerton ,  Ludovic Vincent ,  Lewis J. Kraft ,  Sahngki Hong ,  Boyan Boyanov ,  M. Shane Bowen ,  Sang Park ,  Wayne N. George ,  Andrew A. Brown ,  Dajun Yuan

IPC: C12Q1/68 ,  G03F7/16 ,  C12Q1/6869 ,  C08F220/56 ,  C08G77/04 ,  G03F7/075 ,  G03F7/00 ,  G03F7/004

Abstract: An example of a flow cell includes a substrate; a first primer set attached to a first region on the substrate, the first primer set including an un-cleavable first primer and a cleavable second primer; and a second primer set attached to a second region on the substrate, the second primer set including a cleavable first primer and an un-cleavable second primer.

公开(公告)号：US20210190675A1

公开(公告)日：2021-06-24

申请号：US17126548

申请日：2020-12-18

Applicant: ILLUMINA, INC.

Inventor： Sahngki Hong ,  M. Shane Bowen ,  Lewis J. Kraft

IPC: G01N21/05 ,  G03F7/00 ,  G03F7/09 ,  H01L21/311 ,  H01L21/02 ,  H01L31/08

Abstract: One example of a flow cell includes a base support and a multi-layer stack positioned over the base support. The multi-layer stack includes a resin layer positioned over the base support; and a hydrophobic layer positioned over the resin layer. A depression is defined in the multi-layer stack through the hydrophobic material and through a portion of the resin.

公开(公告)号：US20210139975A1

公开(公告)日：2021-05-13

申请号：US17139834

申请日：2020-12-31

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

Inventor： Jeffrey S. Fisher ,  Brian D. Mather ,  Maria Candelaria Rogert Bacigalupo ,  Justin Fullerton ,  Ludovic Vincent ,  Lewis J. Kraft ,  Sahngki Hong ,  Boyan Boyanov ,  M. Shane Bowen ,  Sang Park ,  Wayne N. George ,  Andrew A. Brown ,  Dajun Yuan

IPC: C12Q1/6869 ,  C08F220/56 ,  C08G77/04 ,  G03F7/075 ,  G03F7/16

Abstract: An example of a flow cell includes a substrate; a first primer set attached to a first region on the substrate, the first primer set including an un-cleavable first primer and a cleavable second primer; and a second primer set attached to a second region on the substrate, the second primer set including a cleavable first primer and an un-cleavable second primer.

公开(公告)号：US20240207842A1

公开(公告)日：2024-06-27

申请号：US18542511

申请日：2023-12-15

Applicant: ILLUMINA, INC.

Inventor： Gianluca Andrea Artioli ,  Johan Sebastian Basuki ,  Boyan Boyanov ,  Gabriele Canzi ,  Sahngki Hong ,  Nam Nguyen ,  Sebastien Georg Gabriel Ricoult ,  Alexandra Szemjonov ,  Xavier von Hatten

IPC: B01L3/00

CPC classification number: B01L3/5027 ,  B01L2200/025 ,  B01L2300/12 ,  B01L2300/16 ,  B01L2300/1805

Abstract: An example of a flow cell includes a substrate; a plurality of reactive regions spatially separated from one another across the substrate; and a plurality of independently removable coatings respectively positioned over each of the plurality of reactive regions. Each of the plurality of reactive regions includes a polymeric hydrogel layer; and a reactive entity attached to the polymeric hydrogel layer. At least one of the independently removable coatings is a gas-dissolvable coating.

公开(公告)号：US20230259034A1

公开(公告)日：2023-08-17

申请号：US18068229

申请日：2022-12-19

Applicant: ILLUMINA, INC.

Inventor： Jeffrey S. Fisher ,  Tanmay Ghonge ,  Sahngki Hong ,  Lewis J Kraft

IPC: G03F7/20

CPC classification number: G03F7/2026 ,  B29C59/026

Abstract: In a method, a resin layer of a stack (e.g., resin layer over sacrificial layer over transparent substrate) is imprinted to form a convex region including first region with first height and second region with second height

公开(公告)号：US20230139821A1

公开(公告)日：2023-05-04

申请号：US17739964

申请日：2022-05-09

Applicant: ILLUMINA, INC.

Inventor： Jeffrey S. Fisher ,  Anthony Flannery ,  Sahngki Hong ,  Brinda Kodira Cariappa ,  Lewis J. Kraft

IPC: B01L3/00 ,  G03F7/039 ,  G03F7/038 ,  G03F7/00

Abstract: In an example method, a first functionalized layer is deposited over a resin layer including multi-depth depressions separated by interstitial regions, each depression including a deep portion and a shallow portion adjacent to the deep portion; a photoresist is deposited over the first functionalized layer; an ultraviolet light dosage is directed, through the resin layer, whereby a first photoresist portion generates an insoluble photoresist and a second photoresist portion becomes a soluble photoresist; the soluble photoresist is removed to expose a portion of the first functionalized layer; the portion of the first functionalized layer is removed to expose a portion of the resin layer; a second functionalized layer is deposited over the insoluble photoresist, and over the exposed portion of the resin layer; the insoluble photoresist is removed to expose the first functionalized layer; and the first functionalized layer or the second functionalized layer is removed from the interstitial regions.

公开(公告)号：US20220390348A1

公开(公告)日：2022-12-08

申请号：US17826091

申请日：2022-05-26

Applicant: ILLUMINA, INC.

Inventor： Eric M. Brustad ,  Craig Michael Ciesla ,  Jeffrey S. Fisher ,  Sahngki Hong ,  Lewis J. Kraft

IPC: G01N15/14 ,  C12Q1/6844 ,  C12Q1/6869

Abstract: An example of a flow cell includes a substrate; a plurality of reactive regions extending along the substrate; and a non-reactive region separating one of the plurality of reactive regions from an adjacent one of the plurality of reactive regions. Each of the plurality of reactive regions includes alternating first and second areas positioned along the reactive region. Each of the first areas includes a first primer set and each of the second areas includes a second primer set that is different than the first primer set. Either adjacent first and second areas directly abut each other, or) the first areas are positioned on protrusions and the second areas are positioned in depressions adjacent to the protrusions.