公开(公告)号：US10919033B2

公开(公告)日：2021-02-16

申请号：US16626393

申请日：2018-12-17

申请人： Illumina, Inc. ,  ILLUMINA CAMBRIDGE LIMITED

发明人： Hongji Ren ,  Jonathan Mark Boutell ,  John A. Moon ,  M. Shane Bowen ,  Alex Nemiroski ,  Gary Mark Skinner ,  Kenny Chen

IPC分类号： C12Q1/6876 ,  B01L3/00 ,  C08F220/60 ,  B01J19/00 ,  C08F220/56 ,  C09D133/26

摘要： In an example of the method, a functionalized coating layer is applied in depressions of a patterned flow cell substrate. The depressions are separated by interstitial regions. A primer is grafted to the functionalized coating layer to form a grafted functionalized coating layer in the depressions. A hydrogel is applied on at least the grafted functionalized coating layer.

公开(公告)号：US20210024991A1

公开(公告)日：2021-01-28

申请号：US16626452

申请日：2019-06-07

申请人： ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

发明人： Jeffrey S. Fisher ,  Brian D. Mather ,  Maria Candelaria Rogert Bacigalupo ,  Justin Fullerton ,  Ludovic Vincent ,  Lewis J. Kraft ,  Sahngki Hong ,  Boyan Boyanov ,  M. Shane Bowen ,  Sang Park ,  Wayne N. George ,  Andrew A. Brown ,  Dajun Yuan

IPC分类号： C12Q1/6869 ,  G03F7/075 ,  C08G77/04 ,  C08F220/56

摘要： An example of a flow cell includes a substrate; a first primer set attached to a first region on the substrate, the first primer set including an un-cleavable first primer and a cleavable second primer; and a second primer set attached to a second region on the substrate, the second primer set including a cleavable first primer and an un-cleavable second primer.

公开(公告)号：US20210187469A1

公开(公告)日：2021-06-24

申请号：US17130489

申请日：2020-12-22

申请人： ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED ,  ILLUMINA SINGAPORE PTE. LTD.

发明人： Wayne N. GEORGE ,  Jonathan Mark BOUTELL ,  Mathieu LESSARD-VIGER ,  Saurabh NIRANTAR ,  Pietro GATTI LAFRANCONI ,  Seth MCDONALD ,  Yin Nah TEO ,  Maria Rogert BACIGALUPO ,  Sergio PEISAJOVICH ,  Eric BRUSTAD ,  Michael HOWARD ,  Xiangyuan YANG ,  M. Shane Bowen ,  Alex NEMIROSKI ,  Jeffrey BRODIN ,  Ludovic VINCENT ,  Lorenzo BERTI ,  Andrew A. BROWN ,  Fei SHEN ,  Lena STORMS ,  Olivia BENICE

IPC分类号： B01J19/00

摘要： Provided is a nanoparticle including a scaffold, a single template site for bonding a template polynucleotide to the scaffold, and a plurality of accessory sites for bonding accessory oligonucleotides to the scaffold, wherein the scaffold is selected from one or more scaffold DNA molecules and one or more scaffold polypeptides, the single template site for bonding a template polynucleotide to the scaffold is selected from a covalent template bonding site and a noncovalent template bonding site and the plurality of accessory sites for bonding accessory oligonucleotides to the scaffold are selected from covalent accessory oligonucleotide bonding sites and noncovalent accessory oligonucleotide bonding sites. Also provided are methods of using the nanoparticle.

公开(公告)号：US20230332224A1

公开(公告)日：2023-10-19

申请号：US18317865

申请日：2023-05-15

申请人： ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

发明人： Jeffrey S. Fisher ,  Brian D. Mather ,  Maria Candelaria Rogert Bacigalupo ,  Justin Fullerton ,  Ludovic Vincent ,  Lewis J. Kraft ,  Sahngki Hong ,  Boyan Boyanov ,  M. Shane Bowen ,  Sang Park ,  Wayne N. George ,  Andrew A. Brown

IPC分类号： C12Q1/6869 ,  C08F220/56 ,  C08G77/04 ,  G03F7/075 ,  G03F7/16

CPC分类号： C12Q1/6869 ,  C08F220/56 ,  C08G77/04 ,  G03F7/0757 ,  G03F7/165 ,  G03F7/0002

摘要： An example of a flow cell includes a substrate; a first primer set attached to a first region on the substrate, the first primer set including an un-cleavable first primer and a cleavable second primer; and a second primer set attached to a second region on the substrate, the second primer set including a cleavable first primer and an un-cleavable second primer.

公开(公告)号：US11760994B2

公开(公告)日：2023-09-19

申请号：US17358892

申请日：2021-06-25

申请人： ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

发明人： Maria Candelaria Rogert Bacigalupo ,  Frank Steemers ,  Jeffrey Fisher ,  Andrew Slatter ,  Lewis Kraft ,  Niall Gormley ,  M. Shane Bowen

IPC分类号： C12N15/10

CPC分类号： C12N15/1065 ,  C12N15/1093

摘要： Provided is a method, including stretching a polynucleotide over a substrate including a plurality of equally spaced cleavage regions including a plurality of transposases, cleaving the polynucleotide with two or more of the plurality of transposases to form a plurality of polynucleotide fragments, and separating, within the plurality of polynucleotide fragments, a population of longer polynucleotide fragments from a population of shorter polynucleotide fragments. Also provided is a method including stretching a polynucleotide over a substrate including a plurality of equally spaced cleavage regions including a plurality of transposases, cleaving the polynucleotide with two or more of the plurality of transposases to form a plurality of polynucleotide fragments, and separating, within the plurality of polynucleotide fragments, a population of longer polynucleotide fragments from a population of shorter polynucleotide fragments.

公开(公告)号：US20210086174A1

公开(公告)日：2021-03-25

申请号：US17114134

申请日：2020-12-07

申请人： ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

发明人： Hongji Ren ,  Jonathan Mark Boutell ,  John A. Moon ,  M. Shane Bowen ,  Alex Nemiroski ,  Gary Mark Skinner ,  Kenny Chen

IPC分类号： B01L3/00 ,  C08F220/60 ,  B01J19/00 ,  C08F220/56 ,  C09D133/26 ,  C12Q1/6876

摘要： In an example of the method, a functionalized coating layer is applied in depressions of a patterned flow cell substrate. The depressions are separated by interstitial regions. A primer is grafted to the functionalized coating layer to form a grafted functionalized coating layer in the depressions. A hydrogel is applied on at least the grafted functionalized coating layer.

公开(公告)号：US20180179575A1

公开(公告)日：2018-06-28

申请号：US15848640

申请日：2017-12-20

申请人： ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

发明人： Wayne N. George ,  Alexandre Richez ,  M. Shane Bowen ,  Andrew A. Brown ,  Dajun M. Yuan ,  Audrey Rose Zak ,  Sean M. Ramirez ,  Raymond Campos

IPC分类号： C12Q1/68 ,  C09D183/06 ,  C09D133/26

摘要： An example of an array includes a support, a cross-linked epoxy polyhedral oligomeric silsesquioxane (POSS) resin film on a surface of the support, and a patterned hydrophobic polymer layer on the cross-linked epoxy POSS resin film. The patterned hydrophobic polymer layer defines exposed discrete areas of the cross-linked epoxy POSS resin film, and a polymer coating is attached to the exposed discrete areas. Another example of an array includes a support, a modified epoxy POSS resin film on a surface of the support, and a patterned hydrophobic polymer layer on the modified epoxy POSS resin film. The modified epoxy POSS resin film includes a polymer growth initiation site, and the patterned hydrophobic polymer layer defines exposed discrete areas of the modified epoxy POSS resin film. A polymer brush is attached to the polymer growth initiation site in the exposed discrete areas.

公开(公告)号：US12110547B2

公开(公告)日：2024-10-08

申请号：US17033237

申请日：2020-09-25

申请人： Illumina, Inc.

发明人： Andrew A. Brown ,  Wayne N. George ,  Alexandre Richez ,  M. Shane Bowen

IPC分类号： G01N33/53 ,  C12Q1/6874 ,  G03F7/00 ,  G03F7/16 ,  B01L3/00 ,  G01N21/64

CPC分类号： C12Q1/6874 ,  G03F7/0002 ,  G03F7/161 ,  B01L3/5085 ,  B01L2200/12 ,  B01L2300/0819 ,  B01L2300/0829 ,  B01L2300/161 ,  G01N21/645

摘要： Substrates comprising dual functional polymer layered surfaces and the preparation thereof by using UV nano-imprinting processes are disclosed. The substrates can be used as flow cells, nanofluidic or microfluidic devices for biological molecules analysis.

公开(公告)号：US12083514B2

公开(公告)日：2024-09-10

申请号：US17727622

申请日：2022-04-22

申请人： ILLUMINA, Inc.

发明人： Maxwell Zimmerley ,  LiangLiang Qiang ,  M. Shane Bowen ,  Steven H. Modiano ,  Dajun Yuan ,  Randall Smith ,  Arthur J. Pitera ,  Hai Quang Tran ,  Gerald Kreindl

IPC分类号： B01L3/00

CPC分类号： B01L3/502715 ,  B01L3/502707 ,  B01L3/502746

摘要： An interposer for a flow cell comprises a base layer having a first surface and a second surface opposite the first surface. The base layer comprises black polyethylene terephthalate (PET). A first adhesive layer is disposed on the first surface of the base layer. The first adhesive layer comprises methyl acrylic adhesive. A second adhesive layer is disposed on the second surface of the base layer. The second adhesive layer comprises methyl acrylic adhesive. A plurality of microfluidic channels extends through each of the base layer, the first adhesive layer, and the second adhesive layer.

公开(公告)号：US20240209412A1

公开(公告)日：2024-06-27

申请号：US18590738

申请日：2024-02-28

申请人： ILLUMINA, INC.

发明人： Wayne N. George ,  Alexandre Richez ,  M. Shane Bowen ,  Andrew A. Brown ,  Dajun Yuan ,  Audrey Rose Zak ,  Sean M. Ramirez ,  Raymond Campos

IPC分类号： C12Q1/68 ,  C08G77/14 ,  C09D133/26 ,  C09D183/06

CPC分类号： C12Q1/68 ,  C09D133/26 ,  C09D183/06 ,  C08G77/14

摘要： An example of an array includes a support, a cross-linked epoxy polyhedral oligomeric silsesquioxane (POSS) resin film on a surface of the support, and a patterned hydrophobic polymer layer on the cross-linked epoxy POSS resin film. The patterned hydrophobic polymer layer defines exposed discrete areas of the cross-linked epoxy POSS resin film, and a polymer coating is attached to the exposed discrete areas. Another example of an array includes a support, a modified epoxy POSS resin film on a surface of the support, and a patterned hydrophobic polymer layer on the modified epoxy POSS resin film. The modified epoxy POSS resin film includes a polymer growth initiation site, and the patterned hydrophobic polymer layer defines exposed discrete areas of the modified epoxy POSS resin film. A polymer brush is attached to the polymer growth initiation site in the exposed discrete areas.