公开(公告)号：US20230407341A1

公开(公告)日：2023-12-21

申请号：US18178675

申请日：2023-03-06

Applicant: The General Hospital Corporation

Inventor： J. Keith Joung ,  Jeffry D. Sander ,  Morgan Maeder ,  Yanfang Fu

IPC: C12N15/90 ,  C12N9/22 ,  C12N15/63 ,  C12N15/85 ,  C07K14/005 ,  C07K14/195 ,  C12N9/02 ,  C12N9/10 ,  C12N9/16 ,  C12N9/96 ,  C12N15/01 ,  C12N15/10 ,  C12N15/11

CPC classification number: C12N15/907 ,  C12N9/22 ,  C12N15/63 ,  C12Y301/21004 ,  C12N15/85 ,  C07K14/005 ,  C07K14/195 ,  C12N9/0071 ,  C12N9/1007 ,  C12N9/16 ,  C12N9/96 ,  C12N15/01 ,  C12N15/102 ,  C12N15/1031 ,  C12N15/11 ,  C07K2319/00 ,  C07K2319/01 ,  C12N2710/00033 ,  C12N2770/00033 ,  C12Y114/11 ,  C12Y201/01 ,  C12Y301/00 ,  C12N2800/80 ,  C12N2310/20 ,  C07K2319/80

Abstract: Methods for increasing specificity of RNA-guided genome editing, e.g., editing using CRISPR/Cas9 systems, using truncated guide RNAs (tru-gRNAs).

公开(公告)号：US20190234948A1

公开(公告)日：2019-08-01

申请号：US16164701

申请日：2018-10-18

Applicant: Aelan Cell Technologies, Inc.

Inventor： Victoria LUNYAK ,  James Robert TOLLERVEY

IPC: G01N33/573 ,  C12N9/10 ,  G01N33/543

CPC classification number: G01N33/573 ,  C07K14/47 ,  C12N9/1007 ,  C12P21/02 ,  C12P21/06 ,  C12Y201/01 ,  C12Y201/01043 ,  G01N33/54306

Abstract: Provided herein are compositions and methods related to the production and detection of a histone H1.0 protein dimethylated at lysine residue 180 (K180) (H1.0K180me2 protein) or a histone H1.0 peptide dimethylated at a lysine residue corresponding to K180 (H1.0K180me2 peptides). The H1.0K180me2 protein and H1.0K180me2 peptides are useful for applications including, but not limited to, molecular diagnostics of DNA damage, genotoxic stress, radiation exposure, and Alzheimer's disease, therapeutics, monitoring of therapeutic regimens, patient stratification, and drug screening. Also provided herein are antibodies specific for the H1.0K180me2 protein and H1.0K180me2 peptides.

公开(公告)号：US20160355542A1

公开(公告)日：2016-12-08

申请号：US14973637

申请日：2015-12-17

Applicant: Timothy H. Bestor ,  Jingyue Ju ,  Xiaoxu Li ,  James J. Russo

Inventor： Timothy H. Bestor ,  Jingyue Ju ,  Xiaoxu Li ,  James J. Russo

IPC: C07H19/16 ,  C12Q1/68 ,  C12N9/10 ,  C12P19/34

CPC classification number: C12Q1/6806 ,  C07H19/16 ,  C07H19/167 ,  C12N9/1007 ,  C12P19/34 ,  C12Q1/6827 ,  C12Q1/6874 ,  C12Y201/01 ,  C12Q2535/101 ,  C12Q2535/122 ,  C12Q2563/101 ,  C12Q2565/631 ,  C12Q2563/167 ,  C12Q2563/107

Abstract: The present invention provides a method of determining whether cytosine residues present at a predetermined positions within a single strand of a double stranded DNA of known sequence are methylated as well as compounds for carrying out this method.

Abstract translation: 本发明提供了确定存在于已知序列的双链DNA的单链内的预定位置处的胞嘧啶残基是否被甲基化的方法以及用于实施该方法的化合物。

公开(公告)号：US09458481B2

公开(公告)日：2016-10-04

申请号：US13806310

申请日：2011-06-16

Applicant: Thilo Hans Winzer ,  Tracy Carol Walker ,  Ian Alexander Graham

Inventor： Thilo Hans Winzer ,  Tracy Carol Walker ,  Ian Alexander Graham

IPC: C07K14/415 ,  C12P17/18 ,  C12N9/10 ,  C12N15/82

CPC classification number: C12P17/188 ,  C07K14/415 ,  C12N9/1007 ,  C12N15/8243 ,  C12Y201/01

Abstract: This disclosure relates to the isolation and sequencing of nucleic acid molecules that encode methyltransferase polypeptides from a Papaver somniferum cultivar; and uses in the production of noscapine and identification of poppy cultivars that include the genes that include these nucleic acid molecules.

Abstract translation: 本公开涉及从罂粟种植体品种编码甲基转移酶多肽的核酸分子的分离和测序; 并用于生产诺斯卡品和鉴定包括这些核酸分子在内的基因的罂粟品种。

公开(公告)号：US20160281118A1

公开(公告)日：2016-09-29

申请号：US15011855

申请日：2016-02-01

Applicant: SYMRISE AG

Inventor： GERHARD KRAMMER ,  JAKOB PETER LEY ,  KATRIN GEIßLER ,  TORSTEN GEIßLER ,  FRAUKE GOMOLL ,  PETER WELTERS ,  GUIDO JACH ,  LUDGER WESSJOHANN

IPC: C12P13/02 ,  C12N9/10 ,  C12P13/00 ,  C12P7/22 ,  C12P7/62

CPC classification number: C12P13/02 ,  A23L27/204 ,  A23L27/24 ,  A23V2002/00 ,  C12N9/1007 ,  C12N9/1085 ,  C12P7/22 ,  C12P7/40 ,  C12P7/42 ,  C12P7/62 ,  C12P13/001 ,  C12Y201/01 ,  C12Y205/01006

Abstract: The invention relates to providing both fermentative and biotechnological methods for producing 3,4-methylized cinnamic acids, 3,4-methylized cinnamic acid esters, 3,4-dimethoxyphenethylamine, and 4-methylized cinnamic acid amides using a 4′-O-methyltransferase, optionally in combination with further enzymes, wherein the enzymes are selected by means of metabolic engineering and operation have been adapted by targeted optimization, and compositions obtained by means of the method.The invention further relates to vector systems, recombinant microorganisms or fungi, and specific nucleic acid segments and polypeptides.

Abstract translation: 本发明还涉及载体系统，重组微生物或真菌，以及特异性核酸区段和多肽。

公开(公告)号：US20160177324A1

公开(公告)日：2016-06-23

申请号：US14787408

申请日：2013-04-28

Applicant: INSTITUTE OF BOTANY, THE CHINESE ACADEMY OF SCIENCES

Inventor： Liangsheng WANG ,  Hui DU ,  Qingyan SHU

IPC: C12N15/82 ,  C12N9/10

CPC classification number: C12N15/825 ,  C12N9/1007 ,  C12N15/8205 ,  C12Y201/01

Abstract: The present invention provides novel protein and gene related to flavonoid O-methyltransferase (FOMT) and their uses therefore. The said protein having an amino acid sequence shown in SEQ ID NO: 3, or an amino acid sequence having deletion, substitution or insertion of one or plural amino acids in said amino acid sequence. The said gene comprising the nucleotide sequence shown in SEQ ID NO: 1, or a gene which hybridizes with said gene under stringent conditions and encodes a protein, which has anthocyanin 3′-O-methyltransferase or 3′,5′-O-methyltransferase activity. The present invention also provides a method for obtaining the transgenic plant used the above-mentioned gene.

Abstract translation: 本发明提供与类黄酮O-甲基转移酶（FOMT）相关的新型蛋白质和基因及其用途。 所述蛋白质具有SEQ ID NO：3所示的氨基酸序列，或在所述氨基酸序列中具有一个或多个氨基酸的缺失，取代或插入的氨基酸序列。 所述基因包含SEQ ID NO：1所示的核苷酸序列，或在严格条件下与所述基因杂交并编码具有花青素3'-O-甲基转移酶或3'，5'-O-甲基转移酶 活动。 本发明还提供了获得使用上述基因的转基因植物的方法。

公开(公告)号：US20160138030A1

公开(公告)日：2016-05-19

申请号：US14899846

申请日：2014-06-20

Applicant: DANISCO US INC.

Inventor： Miles C. SCOTCHER ,  Derek H. WELLS

IPC: C12N15/74 ,  C12N9/10 ,  C12N15/70

CPC classification number: C12N15/74 ,  C12N9/1007 ,  C12N15/70 ,  C12P5/007 ,  C12P7/065 ,  C12Y201/01 ,  Y02E50/17

Abstract: The invention provides compositions and methods for clostridial bacteria that have been engineered to produce and/or to improve efficiency of production of industrial bioproducts.

Abstract translation: 本发明提供了经工程化以生产和/或提高工业生物产品生产效率的梭菌的组合物和方法。

公开(公告)号：US20150203875A1

公开(公告)日：2015-07-23

申请号：US14424404

申请日：2013-08-27

Applicant: Genomatica, Inc.

Inventor： Anthony P. Burgard ,  Robin E. Osterhout ,  Stephen J. Van Dien ,  Cara Ann Tracewell ,  Priti Pharkya ,  Stefan Andrae

IPC: C12P7/18 ,  C12P19/02 ,  C12N9/04 ,  C12N9/88 ,  C12N9/10 ,  C07D207/12 ,  C08G18/32 ,  C08G63/12 ,  C08G63/66 ,  C08G69/26 ,  C08G63/06 ,  C12P7/24 ,  C12N9/06

CPC classification number: C12P7/18 ,  C07D207/12 ,  C08G18/3206 ,  C08G63/06 ,  C08G63/12 ,  C08G63/66 ,  C08G69/26 ,  C12N9/0006 ,  C12N9/0028 ,  C12N9/1007 ,  C12N9/88 ,  C12P7/24 ,  C12P19/02 ,  C12Y101/01224 ,  C12Y101/01244 ,  C12Y101/02007 ,  C12Y105/0102 ,  C12Y201/01 ,  C12Y201/0109 ,  C12Y402/01 ,  Y02P20/52

Abstract: Provided herein is a non-naturally occurring microbial organism having a methanol metabolic pathway (MMP) that can enhance the availability of reducing equivalents in the presence of methanol. Such reducing equivalents can be used to increase the product yield of organic compounds produced by the microbial organism, such as 1,4-butanediol (BDO). Also provided herein are methods for using such an organism to produce BDO.

Abstract translation: 本文提供了具有甲醇代谢途径（MMP）的非天然存在的微生物生物，其可以在甲醇存在下增强还原当量的可用性。 这种还原当量可用于增加由微生物生物例如1,4-丁二醇（BDO）产生的有机化合物的产物产率。 本文还提供了使用这种生物体产生BDO的方法。

公开(公告)号：US20140295556A1

公开(公告)日：2014-10-02

申请号：US14213479

申请日：2014-03-14

Applicant: THE GENERAL HOSPITAL CORPORATION

Inventor： J. Keith Joung ,  Shengdar Tsai

IPC: C12N9/22 ,  C12N15/63

CPC classification number: C12N15/102 ,  C07K14/005 ,  C07K14/195 ,  C07K2319/00 ,  C07K2319/01 ,  C07K2319/80 ,  C12N9/0071 ,  C12N9/1007 ,  C12N9/16 ,  C12N9/22 ,  C12N9/96 ,  C12N15/01 ,  C12N15/1031 ,  C12N15/11 ,  C12N15/63 ,  C12N15/85 ,  C12N2310/20 ,  C12N2710/00033 ,  C12N2770/00033 ,  C12N2800/80 ,  C12Y114/11 ,  C12Y201/01 ,  C12Y301/00 ,  C12Y301/21004

Abstract: Methods for increasing specificity of RNA-guided genome editing, e.g., editing using CRISPR/Cas9 systems.

Abstract translation: 提高RNA指导基因组编辑特异性的方法，例如使用CRISPR / Cas9系统进行编辑。

公开(公告)号：US20100009423A1

公开(公告)日：2010-01-14

申请号：US12489038

申请日：2009-06-22

Applicant: Joseph Chappell ,  Shigeru Okada ,  Scott Kinison ,  Tom Niehaus

Inventor： Joseph Chappell ,  Shigeru Okada ,  Scott Kinison ,  Tom Niehaus

IPC: C12P5/02 ,  C12N9/10 ,  C12N15/11 ,  C12N15/00 ,  C12N1/21 ,  C12N1/15 ,  C12N5/04 ,  C12N5/06 ,  C12N1/12

CPC classification number: C12N9/1007 ,  C12N15/8243 ,  C12P5/007 ,  C12Y201/01

Abstract: Provided is an isolated polypeptide having triterpene methyltransferase activity. Also provided is an isolated nucleic acid molecule that encodes the triterpene methyltransferase polypeptides; a vector comprising the nucleic acid molecules that encode the triterpene methyltransferase polypeptides; and a host cell(s) transfected with the aforementioned nucleic acid molecule or vector. In another aspect, a method of producing a methylated triterpene is provided. The method comprises providing a metabolizable carbon source to a host cell transfected with a nucleic acid molecule that encodes a triterpene methyltransferase under conditions sufficient for production of a methylated triterpene. The method optionally further comprises isolating the methylated triterpene produced by the host cell.

Abstract translation: 提供了具有三萜甲基转移酶活性的分离的多肽。 还提供了编码三萜甲基转移酶多肽的分离的核酸分子; 包含编码三萜甲基转移酶多肽的核酸分子的载体; 和用上述核酸分子或载体转染的宿主细胞。 另一方面，提供了生产甲基化三萜的方法。 该方法包括向在用于生产甲基化三萜烯的条件下编码三萜甲基转移酶的核酸分子转染的宿主细胞提供可代谢碳源。 所述方法任选进一步包括分离由宿主细胞产生的甲基化三萜烯。