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49 条记录 (耗时 0.016 秒)

    Biologically active factor
    11.
    发明授权
    Biologically active factor 失效
    生物活性因子

    公开(公告)号：US5294544A

    公开(公告)日：1994-03-15

    申请号：US887681

    申请日：1992-05-22

    申请人： Patrick S. Gentile ,  Charlie R. Mantel ,  Hal E. Broxmeyer

    发明人： Patrick S. Gentile ,  Charlie R. Mantel ,  Hal E. Broxmeyer

    IPC分类号： A61K38/00 ,  C07K14/47 ,  C07K14/52 ,  C12P21/00 ,  C12N5/06

    CPC分类号： C07K14/52 ,  C07K14/4703 ,  A61K38/00 ,  Y10S530/829 ,  Y10S530/838

    摘要： The invention comprises a factor having the following characteristics:a) It inhibits granulocyte-macrophage colony and cluster formation;b) It has a molecular weight of about 8 kDa as determined by SDS-PAGE;c) It has a weak anionic charge at pH 7.4 as shown by anion exchange chromatography;d) It has a flattened isoelectric titration curve as shown by anion exchange chromatography; andd) It is a protein.The invention also comprises methods of making and using the factor and compositions comprising the factor.

    摘要翻译： 本发明包括具有以下特征的因素：a）抑制粒细胞 - 巨噬细胞集落和簇形成; b）通过SDS-PAGE测定其具有约8kDa的分子量; c）如阴离子交换色谱所示，其在pH 7.4下具有弱阴离子电荷; d）阴离子交换层析显示了等电点滴定曲线; 和d）它是蛋白质。 本发明还包括制备和使用包含因子的因子和组合物的方法。

    Augmentation of the production of antibodies in animals and humans and the collection thereof
    15.
    发明授权
    Augmentation of the production of antibodies in animals and humans and the collection thereof 失效
    动物和人体抗体生产的收获及其收集

    公开(公告)号：US3719182A

    公开(公告)日：1973-03-06

    申请号：US3719182D

    申请日：1971-04-22

    申请人： BIO RESPONSE INC

    发明人： ROSE S

    IPC分类号： C07K16/00 ,  C07K16/06 ,  A61B19/00

    CPC分类号： C07K16/06 ,  C07K16/00 ,  Y10S530/806 ,  Y10S530/838

    摘要： IT HAS BEEN FOUND THAT VERY LARGE PRODUCTION OF ANTIBODIES CAN BE ACHIEVED BY REMOVING SPECIFIC FEEDBACK REGULATORY ANTIBODIES BY MEANS OF A LYMPHORESIS PERFORMED UNDER SPECIAL CONDITIONS IN A PATIENT OR SUBJECT (E.G., AN ANIMAL OR HUMAN) WITH INDUCED ANATOMICAL AND PHYSIOLOGICAL CHANGES. THE SUBJECT IS FIRST GIVEN ANTIGEN ADMINISTRATION THEN, PREFERABLY, BUT NOT MANDATORILY, IS SPLENECTOMIZED. A THORACIC DUCT FISTULA IS NEXT PERFORMED. THE CENTRAL VENOUS SYSTEM PRESSURE IS THE PREFERABLY RAISED SO THAT IT IS ABOVE THE ATMOSPHERIC PRESSURE OF THE THORACIC DUCT. IN THIS MANNER, SUBSTANTIALLY ALL THE LYMPTH FLUID IS ALLOWED TO FLOW OUT OF THE THORACIC DUCT FROM THE FIISTULA (THROUGH AN INDWELLING CATHETER) FOR A PROLONGED PERIOD OF TIME. THE LYMPH IS SEPARATED INTO CELLS AND LYMPH FLUID. THE CELLS ARE RETURNED TO THE SUBJECT INTRAVENOUSLY. THE SUBJECT MUST BE GIVEN REPLACMENT FLUID, WHICH CAN BE OF SEVERAL KINDS, BUT ALL LACKING THE SPECIFIC ANTIBODY. BY VIRTUE OF THE ABOVE PROCEDURE, THE PLASMA AND EXTRACELLULAR FLUID OF THE SUBJECT IS CONTINUOUSLY DEPLETED OF FEEDBACK ANTIBODY BECAUSE THE ANTIBODY IS CONTINUOUSLY REMOVED PRIOR TO ITS REACHING THE BLOOD STREAM. BECAUSE OF THIS LACK OF ANTIBODY, IN THE PRESENCE OF ANTIGEN ADMINISTRATION, IT IS FOUND THAT THE ANTIBODY PRODUCTION IN THE LYMPHOID TISSUE AND THEREFORE ITS CONTENT IN THE LYMPH FLUID IS ENORMOUS AND EVER-INCREASES. THE TREMENDOUS INCREASE IN ANTIBODY PRODUCTION APPEARS TO BE SEVERAL ORDERS OF MAGNITUDE GREATER THAN OTHER MODES OF ANTIBODY PRODUCTION AND THEREFORE APPEARS TO HAVE VERY SUBSTANTIAL UTILITY IN THE FIELDS OF BIOLOGY, CHEMISTRY AND VETERINARY AND CLINICAL MEDICINE.

    Growth medium containing a complex of prealbumin, a retinoid and retinol-binding protein used to enhance B cell growth
    17.
    发明授权
    Growth medium containing a complex of prealbumin, a retinoid and retinol-binding protein used to enhance B cell growth 失效
    生长培养基含有前白蛋白，类视色素和视黄醇结合蛋白的复合物，用于增强B细胞的生长

    公开(公告)号：US5518914A

    公开(公告)日：1996-05-21

    申请号：US456644

    申请日：1995-06-02

    申请人： Jochen Buck ,  Urlich Hammerling

    发明人： Jochen Buck ,  Urlich Hammerling

    IPC分类号： A61K31/07 ,  C07K14/475 ,  C07K14/76 ,  C07K19/00 ,  C12N5/0781 ,  C12N5/00 ,  C12P21/04

    CPC分类号： C12N5/0635 ,  A61K31/07 ,  C07K14/475 ,  C07K14/76 ,  C07K19/00 ,  C12N2500/38 ,  C12N2500/84 ,  C12N2500/90 ,  C12N2501/385 ,  Y10S530/837 ,  Y10S530/838

    摘要： This invention provides a complex consisting essentially of prealbumin, retinol-binding protein, and a retinoid which binds to retinol-binding protein, wherein the concentration of retinoid in the complex is from about 2.times.10.sup.-5 M to about 10.sup.-10 M and the concentration of prealbumin and retinol-binding protein is sufficient to maintain the retinoid concentration from about 2.times.10.sup.-5 M to about 10.sup.-10 M. This invention also provides a composition for enhancing the growth of human B cells in culture which comprises an amount of the complex of this invention effective to enhance the growth of human B cells and a nutrient medium. This invention further provides a method of enhancing growth of human B cells in culture comprising growing the cells in culture in a growth medium containing a concentration of the complex effective to enhance growth of the cells. This invention also provides a synthetic growth medium for culturing animal cells comprising the complex of this invention at a concentration effective to enhance cell growth. This invention also provides a method of preventing the growth of human tumor cells containing growth factor which comprises removing the growth factor from the human tumor cells.

    摘要翻译： 本发明提供了一种基本上由前白蛋白，视黄醇结合蛋白和结合视黄醇结合蛋白的类视色素组成的复合物，其中复合物中类视黄醇的浓度为约2×10-5M至约10-10M，并且前白蛋白 和视黄醇结合蛋白足以将类视黄醇浓度从约2×10-5M维持至约10-10M。 本发明还提供了用于增强培养物中人B细胞生长的组合物，其包含一定量的本发明的复合物，其有效增强人B细胞和营养培养基的生长。 本发明进一步提供一种增强培养物中人B细胞生长的方法，包括在含有有效增强细胞生长的复合物浓度的生长培养基中培养培养细胞。 本发明还提供了一种用于培养包含本发明的复合物的动物细胞的合成生长培养基，其浓度有效地增强细胞生长。 本发明还提供一种防止含有生长因子的人肿瘤细胞生长的方法，其包括从人肿瘤细胞中除去生长因子。

    Protein and process for isolating it
    20.
    发明授权
    Protein and process for isolating it 失效
    蛋白质及其分离方法

    公开(公告)号：US4402872A

    公开(公告)日：1983-09-06

    申请号：US357077

    申请日：1982-03-11

    申请人： Hans Bohn

    发明人： Hans Bohn

    IPC分类号： G01N33/53 ,  A61K35/50 ,  A61K38/16 ,  A61K39/00 ,  C07K1/22 ,  C07K14/00 ,  C07K14/47 ,  C07K16/18 ,  G01N33/68 ,  C07G7/00 ,  A61K35/42 ,  A61K39/395

    CPC分类号： C07K14/4715 ,  C07K16/18 ,  G01N33/689 ,  Y10S530/806 ,  Y10S530/838 ,  Y10S530/844 ,  Y10S530/848 ,  Y10S530/851

    摘要： What are disclosed are an isolated tissue protein, denominated PP16, which can be used to prepare anti-sera useful as diagnostic agents, said protein being found in extracts of some human organs such as the placenta, spleen, stomach, and lung and being capable of isolation therefrom and which has the following properties:(a) a carbohydrate content of 4.3.+-.1.6%, comprising 3.4.+-.1.2% of hexoses, 0.24.+-.0.07% hexosamines, 0.06.+-.0.03% of fucose, and 0.6.+-.0.3% of neuraminic acid;(b) a sedimentation coefficient S.sub.20,w.sup.0 of 4.6.+-.0.4 S;(c) a molecular weight, determined in a polyacrylamide gel containing sodium dodecyl-sulfate, of 46,000.+-.3,000;(d) an extinction coefficient E.sub.1 cm.sup.1% (280 nm) of 8.82.+-.0.5;(e) an electrophoretic mobility in the range of albumin; and(f) an isoelectric point of 4.7.+-.0.2.

    摘要翻译： 所公开的是分离的组织蛋白，称为PP16，其可用于制备用作诊断剂的抗血清，所述蛋白质存在于一些人体器官的提取物中，例如胎盘，脾脏，胃和肺，并且能够 其具有以下性质：（a）碳水化合物含量为4.3 +/- 1.6％，包含3.4 +/- 1.2％的己糖，0.24 +/- 0.07％的己糖胺，0.06 +/- 0.03％的岩藻糖 ，0.6±0.3％的神经氨酸; （b）沉降系数S20，w0为4.6 +/- 0.4S; （c）在含有十六烷基硫酸钠的聚丙烯酰胺凝胶中测定的分子量为46,000 +/- 3,000; （d）消光系数E1cm1％（280nm）为8.82 +/- 0.5; （e）白蛋白范围内的电泳迁移率; 和（f）等电点为4.7 +/- 0.2。