利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

227 条记录 (耗时 0.035 秒)

    Yeast-based therapeutic for chronic hepatitis C infection
    11.
    发明授权
    Yeast-based therapeutic for chronic hepatitis C infection 失效
    基于酵母的慢性丙型肝炎感染治疗

    公开(公告)号：US07439042B2

    公开(公告)日：2008-10-21

    申请号：US11254252

    申请日：2005-10-18

    申请人： Richard C. Duke ,  Alex Franzusoff ,  Aurelia Haller ,  Thomas H. King

    发明人： Richard C. Duke ,  Alex Franzusoff ,  Aurelia Haller ,  Thomas H. King

    IPC分类号： C12N15/33

    CPC分类号： C07K14/005 ,  A61K39/00 ,  A61K39/12 ,  A61K39/29 ,  A61K2039/523 ,  A61K2039/5252 ,  A61K2039/55516 ,  C12N2770/24222 ,  C12N2770/24234

    摘要： Disclosed are compositions, including vaccines, and methods for vaccinating an animal against hepatitis C virus (HCV) and for treating or preventing hepatitis C viral infection in an animal. The invention includes a variety of novel HCV fusion proteins that can be used directly as a vaccine or in conjunction with a yeast-based vaccine vehicle to elicit an immune response against HCV in an animal. The invention also includes the use of the HCV fusion gene and protein described herein in any diagnostic or therapeutic protocol for the detection and/or treatment or prevention of HCV infection.

    摘要翻译： 公开了包括疫苗和疫苗接种丙型肝炎病毒（HCV）的组合物和用于治疗或预防动物中的丙型肝炎病毒感染的组合物。 本发明包括可以直接用作疫苗或与基于酵母的疫苗载体一起使用以在动物中引发针对HCV的免疫应答的多种新型HCV融合蛋白。 本发明还包括本文所述的HCV融合基因和蛋白质在任何诊断或治疗方案中用于检测和/或治疗或预防HCV感染的用途。

    Chimeric infectious DNA clones, chimeric porcine circoviruses and uses thereof
    12.
    发明申请
    Chimeric infectious DNA clones, chimeric porcine circoviruses and uses thereof 有权
    嵌合感染性DNA克隆，嵌合型猪圆环病毒及其用途

    公开(公告)号：US20080226666A1

    公开(公告)日：2008-09-18

    申请号：US11893182

    申请日：2007-08-15

    申请人： Xiang-Jin Meng ,  Martijn Fenaux ,  Patrick G. Halbur

    发明人： Xiang-Jin Meng ,  Martijn Fenaux ,  Patrick G. Halbur

    IPC分类号： A61K39/12 ,  C07K2/00 ,  C12N15/33 ,  C12N15/63 ,  C12P21/00

    CPC分类号： A61K39/12 ,  A61K2039/5252 ,  A61K2039/5254 ,  A61K2039/5256 ,  A61K2039/53 ,  A61K2039/54 ,  A61K2039/552 ,  C07K14/005 ,  C12N7/00 ,  C12N2750/10022 ,  C12N2750/10034 ,  C12N2750/10061

    摘要： The present invention relates to infectious DNA clones, infectious chimeric DNA clones of porcine circovirus (PCV), vaccines and means of protecting pigs against viral infection or postweaning multisystemic wasting syndrome (PMWS) caused by PCV2. The new chimeric infectious DNA clone and its derived, avirulent chimeric virus are constructed from the nonpathogenic PCV1 in which the immunogenic ORF gene of the pathogenic PCV2 replaces a gene of the nonpathogenic PCV1, preferably in the same position. The chimeric virus advantageously retains the nonpathogenic phenotype of PCV1 but elicits specific immune responses against the pathogenic PCV2. The invention further embraces the immunogenic polypeptide expression products. In addition, the invention encompasses two mutations in the PCV2 immunogenic capsid gene and protein, and the introduction of the ORF2 mutations in the chimeric clones.

    摘要翻译： 本发明涉及感染性DNA克隆，猪圆环病毒感染性嵌合DNA克隆（PCV），疫苗和保护猪免受病毒感染或PCV2引起的断奶后多系统消耗综合征（PMWS）的方法。 新的嵌合感染性DNA克隆及其衍生的无毒性嵌合病毒由非致病性PCV1构建，其中病原性PCV2的免疫原性ORF基因替代非致病性PCV1的基因，优选在相同的位置。 嵌合病毒有利地保留了PCV1的非病原性表型，但引发针对致病性PCV2的特异性免疫应答。 本发明还包括免疫原性多肽表达产物。 此外，本发明包括PCV2免疫原性衣壳蛋白基因和蛋白质中的两个突变，以及在嵌合克隆中引入ORF2突变。

    Nucleotide sequences of 2S albumin gene and its promoter from grape and uses thereof
    14.
    发明授权
    Nucleotide sequences of 2S albumin gene and its promoter from grape and uses thereof 失效
    2S白蛋白基因及其来自葡萄的启动子的核苷酸序列及其用途

    公开(公告)号：US07250296B2

    公开(公告)日：2007-07-31

    申请号：US10382066

    申请日：2003-03-05

    申请人： Zhijian T. Li ,  Dennis J. Gray

    发明人： Zhijian T. Li ,  Dennis J. Gray

    IPC分类号： C12N15/82 ,  C12N15/33 ,  A01H5/00

    CPC分类号： C12N15/8234 ,  C07K14/415

    摘要： The subject invention pertains to the isolation of nucleotide sequences of the 2S albumin gene and its promoter from grape. Promoter sequences of the invention provide for seed-specific transcription of operably linked polynucleotide sequences. Seed-specific expression activity of the subject promoter was also characterized by using transient green fluorescent protein (GFP) expression analysis in transformed somatic embryos (SE) of grape (cv. Thompson Seedless). The subject invention also concerns expression constructs comprising a promoter of the present invention. Plants, plant tissues, and plant cells bred to contain or transformed with a polynucleotide of the invention are also contemplated by the present invention.

    摘要翻译： 本发明涉及从葡萄分离2S白蛋白基因及其启动子的核苷酸序列。 本发明的启动子序列提供可操作地连接的多核苷酸序列的种子特异性转录。 通过在葡萄转化的体细胞胚（SE）（cv.Thopson Seedless）中使用瞬时绿色荧光蛋白（GFP）表达分析，也表征了本发明启动子的种子特异性表达活性。 本发明还涉及包含本发明的启动子的表达构建体。 繁殖含有或用本发明的多核苷酸转化的植物，植物组织和植物细胞也被本发明考虑。

    Isolation of proteins involved in posttranscriptional gene silencing and methods of use
    16.
    发明授权
    Isolation of proteins involved in posttranscriptional gene silencing and methods of use 有权

    公开(公告)号：US07001739B2

    公开(公告)日：2006-02-21

    申请号：US10226715

    申请日：2002-08-23

    申请人： T. Erik Mirkov ,  Ivan L. Ingelbrecht

    发明人： T. Erik Mirkov ,  Ivan L. Ingelbrecht

    IPC分类号： G01N33/569 ,  G01N33/53 ,  C12N15/33

    CPC分类号： C12N9/22 ,  C12N15/8218 ,  C12N15/8283

    摘要： The present invention includes a method for detecting and isolating sugarcane proteins that interact with the HC-Pro and P1 proteins of SrMV and other proteins involved in gene silencing, particularly in sugarcane. The method uses a two hybrid assay with an HC-Pro, P1, or other silencing-related protein-containing bait protein and a prey protein containing a polypeptide encoded by a DNA molecule in a cDNA library. The method also includes identification of false positives through reverse two-hybrid assays and using in vitro techniques such as farwestern blots or pull down assays where plant physiological conditions may be replicated. Finally, interactions may be confirmed in planta. Some novel proteins used in and discovered using the these methods are also identified. Methods of using viral and plant proteins to regulate silencing in plants such as sugarcane are also discussed.

    Chicken anemia virus mutants and vaccines and uses based on the viral proteins VP1, VP2 and VP3 or sequences of that virus coding therefor
    18.
    发明申请
    Chicken anemia virus mutants and vaccines and uses based on the viral proteins VP1, VP2 and VP3 or sequences of that virus coding therefor 失效
    基于病毒蛋白VP1，VP2和VP3或其编码的病毒序列的鸡贫血病毒突变体和疫苗和用途

    公开(公告)号：US20050100552A1

    公开(公告)日：2005-05-12

    申请号：US09740676

    申请日：2000-12-18

    申请人： Matheus Noteborn ,  Guus Koch

    发明人： Matheus Noteborn ,  Guus Koch

    IPC分类号： A61K38/00 ,  A61K39/00 ,  A61K39/12 ,  A61P31/20 ,  C07H21/04 ,  C07K14/005 ,  C07K14/01 ,  C12N5/06 ,  C12N7/01 ,  C12N15/33 ,  C12N15/86 ,  C12Q1/70 ,  G01N33/569

    CPC分类号： C07K14/005 ,  A61K38/00 ,  A61K39/00 ,  C12N15/86 ,  C12N2750/10022 ,  C12N2750/14143 ,  C12N2830/00 ,  C12N2830/60 ,  G01N33/56983

    摘要： The coding information for three putative chicken anemia virus proteins (VP1, VP2, VP3) was inserted into a baculovirus vector and expressed in insect cells. The immunogenic properties of the chicken anemia virus (CAV) proteins produced separately or together in insect-cell cultures were analyzed by inoculating them into chickens. Only lysates of insect cells which have synthesized equivalent amounts of all three recombinant CAV proteins or cells which synthesized mainly VP1 plus VP2 induced neutralizing antibodies directed against CAV in inoculated chickens. Progeny of those chickens were protected against clinical disease after CAV challenge. Inoculation of a mixture of lysates of cells that were separately infected with VP1-, VP2- and VP3-recombinant baculovirus did not induce significant levels of neutralizing antibody directed against CAV and their progeny were not protected against CAV challenge. Our results indicate that expression in the same cell of at least two CAV proteins, VP1 plus VP2, is required to obtain sufficient protection in chickens. Therefore, recombinant CAV proteins produced by baculovirus vectors can be used as a sub-unit vaccine against CAV infections.

    摘要翻译： 将三种推定的鸡贫血病毒蛋白（VP1，VP2，VP3）的编码信息插入杆状病毒载体中，并在昆虫细胞中表达。 通过将它们接种到鸡中来分析在昆虫细胞培养物中单独或一起产生的鸡贫血病毒（CAV）蛋白的免疫原性。 只有合成了相当量的所有三种重组CAV蛋白或主要合成VP1加VP2的细胞的昆虫细胞裂解液才能诱导接种鸡中抗CAV的中和抗体。 在CAV攻击后，这些鸡的后代受到临床疾病的保护。 分别感染VP1，VP2和VP3-重组杆状病毒的细胞裂解液的混合物接种不诱导针对CAV的显着水平的中和抗体，并且其后代不受CAV攻击的保护。 我们的研究结果表明，需要在至少两种CAV蛋白VP1加VP2的相同细胞中表达以获得足够的鸡保护。 因此，由杆状病毒载体产生的重组CAV蛋白可用作针对CAV感染的亚单位疫苗。

    Grapevine leafroll virus (type 2) proteins and their uses
    19.
    发明授权
    Grapevine leafroll virus (type 2) proteins and their uses 失效
    葡萄卷叶病毒（2型）蛋白质及其用途

    公开(公告)号：US06858426B1

    公开(公告)日：2005-02-22

    申请号：US09613486

    申请日：2000-07-11

    申请人： Hai-Ying Zhu ,  Kai-Shu Ling ,  Dennis Gonsalves

    发明人： Hai-Ying Zhu ,  Kai-Shu Ling ,  Dennis Gonsalves

    IPC分类号： C07K14/08 ,  C12N15/33 ,  C12N15/40 ,  C12N15/82 ,  C12Q1/70 ,  G01N33/569 ,  C12N15/00 ,  C07H21/02 ,  C12N1/20 ,  C12N5/00 ,  C12P21/06

    CPC分类号： C07K14/005 ,  C12N15/8283 ,  C12N2770/00022 ,  C12Q1/70 ,  G01N33/56961 ,  G01N33/56983 ,  G01N2333/08

    摘要： The present invention relates to isolated proteins or polypeptides of grapevine leafroll virus (type 2). The encoding DNA molecules either alone in isolated form or in an expression system, a host cell, or a transgenic grape plant are also disclosed. Other aspects of the present invention relates to a method of imparting grapevine leafroll resistance, to grape and tobacco plants by transforming them with the DNA molecules of the present invention, a method of imparting beet yellows virus resistance to a beet plant, a method of imparting tristeza virus resistance to a citrus plant, and a method of detecting the presence of a grapevine leafroll virus, such as GRLaV-2, in a sample.

    摘要翻译： 本发明涉及葡萄卷叶病毒的分离蛋白质或多肽（2型）。 也公开了分离形式或表达系统中的单独编码DNA分子，宿主细胞或转基因葡萄植物。 本发明的其他方面涉及通过用本发明的DNA分子转化葡萄和烟草植物来赋予葡萄和卷烟抗性的方法，赋予甜菜植物甜菜黄病毒抗性的方法，赋予甜菜甜菜植物的方法 对柑橘类植物的病毒抗性，以及检测样品中葡萄卷叶病毒如GRLaV-2的存在的方法。