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    MIDDLE EAR MUCOSA-LIKE CELL SHEET, PROCESS OF PRODUCING THE SAME AND METHOD OF USING THE SAME
    26.
    发明申请
    MIDDLE EAR MUCOSA-LIKE CELL SHEET, PROCESS OF PRODUCING THE SAME AND METHOD OF USING THE SAME 审中-公开
    中等耳状细胞片及其制造方法及其使用方法

    公开(公告)号：US20160220356A1

    公开(公告)日：2016-08-04

    申请号：US15098604

    申请日：2016-04-14

    申请人： Tokyo Women's Medical University ,  The Jikei University ,  CellSeed Inc.

    发明人： Takanori Hama ,  Hiromi Kojima ,  Hiroshi Moriyama ,  Masayuki Yamato ,  Teruo Okano

    IPC分类号： A61F2/18 ,  A61L27/38

    CPC分类号： A61F2/18 ,  A61F2002/183 ,  A61F2310/00365 ,  A61K35/12 ,  A61L27/3804 ,  A61L27/3813 ,  A61L27/3839 ,  A61L27/3886 ,  A61L2430/14 ,  C12N5/062 ,  C12N2533/30

    摘要： The purpose of the present invention is to obtain an alternative to a substitute of the mucosa in the middle ear which is engrafted on the surface of the bone in the middle ear, hyperplasia of the granulation tissue and the bone and the development of the fibroblast cells in the middle ear are suppressed, and to obtain a middle ear mucosa-like cell sheet retaining cilia in the surface layer, comprising culturing nasal epithelium cells on a cell culture substrate coated with a polymer whose hydration force changes within a temperature range of 0 to 80° C., wherein the cells are cultured within a temperature range where the hydration force of the polymer is weak, and then changing the temperature to a temperature at which the hydration force is strong to recover the cultured cell sheet.

    摘要翻译： 本发明的目的是获得中耳粘膜替代物，其中植入中耳骨的表面，造粒组织和骨的增生以及成纤维细胞的发育 在中耳被抑制，并且获得在表层中保留纤毛的中耳粘膜样细胞片，包括在涂覆有聚合物的细胞培养基质上培养鼻上皮细胞，其水合力在0至 80℃，其中在聚合物的水合力弱的温度范围内培养细胞，然后将温度改变到水合力强的温度以回收培养的细胞片。

    Cultured periodontal ligament cell sheet, process for producing the same and method of use thereof
    27.
    发明授权
    Cultured periodontal ligament cell sheet, process for producing the same and method of use thereof 有权
    培养的牙周膜细胞片，其制造方法及其使用方法

    公开(公告)号：US09114192B2

    公开(公告)日：2015-08-25

    申请号：US11587427

    申请日：2005-04-25

    申请人： Teruo Okano ,  Masateru Hasegawa ,  Masayuki Yamato ,  Akihiko Kikuchi ,  Isao Ishikawa

    发明人： Teruo Okano ,  Masateru Hasegawa ,  Masayuki Yamato ,  Akihiko Kikuchi ,  Isao Ishikawa

    IPC分类号： A61K6/00 ,  A61L27/38 ,  A61K35/32 ,  C12N5/077 ,  A61C8/02

    CPC分类号： A61L27/3886 ,  A61C8/0006 ,  A61K35/32 ,  A61L27/3804 ,  A61L27/3865 ,  C12N5/066 ,  C12N2502/097 ,  C12N2502/1311 ,  C12N2513/00 ,  C12N2539/10

    摘要： A cultured periodontal membrane cell sheet, produced through a process comprising culturing cells on a cell culture support composed of a base material having its surface coated with a temperature-responsive polymer of 0˚C to 80˚C upper limit or lower limit critical dissolution temperature in water under specified conditions, (1) regulating the temperature of culture fluid to temperature≧the upper limit critical dissolution temperature or≦the lower limit critical dissolution temperature, (2) causing a cultured periodontal membrane cell sheet resulting from the culturing to adhere to a carrier, and (3) detaching the sheet together with the carrier. This cultured periodontal membrane cell sheet has a syndesmotic microstructure, exhibiting extremely high bioadherence to the surface of dental root, and realizes high-density transplant of target cells and positive reconstruction of periodontal tissue. Moreover, through laminating of cell sheets to be transplanted and donating of three-dimensional polarity, highly efficient reconstruction of attachment apparatus can be accomplished, and clinical application thereof to moderate periodontitis, severe periodontitis, gingival recession, etc. is highly promising.

    摘要翻译： 一种培养的牙周膜细胞片，其通过以下方法制造，所述方法包括在细胞培养载体上培养细胞，所述细胞培养载体由其表面涂覆有温度响应性聚合物的基底材料构成，所述温度响应性聚合物为0℃至80℃上限或下限临界溶解温度 在规定条件下的水中，（1）将培养液的温度调节至温度≧上限临界溶解温度或下限临界溶解温度，下限临界溶解温度，（2）使由培养产生的培养的牙周膜细胞片粘附于 载体，和（3）与载体一起分离片材。 该培养的牙周膜细胞片具有合成微观结构，对牙根表面具有极高的生物相干性，实现了靶细胞的高密度移植和牙周组织的积极重建。 此外，通过层叠待移植的细胞片和三维极性的提供，可以实现附着装置的高效重建，并且其适度的牙周炎，严重牙周炎，牙龈退缩等的临床应用是非常有希望的。

    Harvested sample preparation personal box and system and method of harvested sample preparation
    28.
    发明授权
    Harvested sample preparation personal box and system and method of harvested sample preparation 有权
    收集的样品制备个人箱和收获样品制备的系​​统和方法

    公开(公告)号：US09012211B2

    公开(公告)日：2015-04-21

    申请号：US13376973

    申请日：2010-06-22

    申请人： Manabu Mizutani ,  Yoshiko Nohmi ,  Kenji Yoneda

    发明人： Manabu Mizutani ,  Yoshiko Nohmi ,  Kenji Yoneda

    IPC分类号： C12M1/00 ,  C12M3/00 ,  C12M1/22 ,  A61B10/00 ,  C12M1/12

    CPC分类号： C12M45/22 ,  A61B10/0096 ,  C12M37/00

    摘要： A harvested sample preparation system includes an operation isolator 3 in which aseptic manipulation is executed, a harvested sample preparation personal box 4 which can be connected with the operation isolator 3 and a storage 5 that stores a plurality of harvested sample preparation personal boxes 4. The harvested sample preparation personal box 4 includes a first housing chamber 4Aa and a second housing chamber 4Ab, and a fluid appropriate for cell culturing is supplied to the first housing chamber 4Aa from a fluid supply pipe 16 included by the storage 5 while cooling fluid is supplied to the second housing chamber 4Ab from a cooling fluid supply pipe 18. The harvested sample preparation system that prevents mix-up and cross-contamination with a simple configuration can be provided.

    摘要翻译： 收集的样品制备系统包括其中执行无菌操作的操作隔离器3，可与操作隔离器3连接的收集的样品制备个人盒4和存储多个收获的样品制备个人盒的存储器5。 收集的样品制备个人箱4包括第一容纳室4Aa和第二容纳室4Ab，并且适合于细胞培养的流体从包含在储存器5中的流体供应管16供应到第一容纳室4Aa，同时供应冷却流体 从冷却流体供给管18到第二容纳室4Ab。可以提供防止混合和交叉污染的收集的样品制备系统，其具有简单的构造。

    CELL SHEET TRANSPLANTATION DEVICE AND METHOD FOR USING THE SAME
    29.
    发明申请
    CELL SHEET TRANSPLANTATION DEVICE AND METHOD FOR USING THE SAME 审中-公开
    细胞移植装置及其使用方法

    公开(公告)号：US20150032223A1

    公开(公告)日：2015-01-29

    申请号：US14008769

    申请日：2012-04-02

    申请人： Shigeru Miyagawa ,  Atsuhiro Saito ,  Yoshiki Sawa ,  Manabu Mizutani ,  Akima Harada

    发明人： Shigeru Miyagawa ,  Atsuhiro Saito ,  Yoshiki Sawa ,  Manabu Mizutani ,  Akima Harada

    IPC分类号： A61F2/02

    CPC分类号： A61F2/02 ,  A61F2230/0006 ,  A61L27/38 ,  A61L27/52 ,  C12M25/02 ,  C12M33/00

    摘要： A cell sheet transplantation device having a plane for transplanting a sheet of cultured cells, the device comprising, in the plane in the same direction, (1) a planar surface portion for capturing a cell sheet while maintaining a sheet-shaped form, and (2) suction holes for immobilizing a transplantation site by suction, the suction holes being positioned around the planar surface portion. By using the cell sheet transplantation device, a cultured cell sheet can be detached effectively, and the detached cultured cell sheet can be transplanted in an effective and simple manner.

    摘要翻译： 一种具有用于移植培养细胞片的平面的细胞移植装置，所述装置在相同方向的平面中包括（1）用于在保持片状形状的同时捕获细胞片的平面部分，以及（ 2）用于通过抽吸固定移植部位的吸孔，吸孔位于平面部分周围。 通过使用细胞移植装置，可以有效地剥离培养的细胞片，并且可以有效且简单地移植分离的培养细胞片。