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    System and method incorporating solid buffer
    23.
    发明授权
    System and method incorporating solid buffer 有权

    公开(公告)号：US11131679B2

    公开(公告)日：2021-09-28

    申请号：US16149799

    申请日：2018-10-02

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： James A. Ball ,  Jonathan Schultz

    IPC: G01N33/84 ,  B01J19/00 ,  C12Q1/68 ,  C12Q1/6869

    Abstract: A buffered suspension includes a surfactant and a solid buffer particulate having a point of zero charge at least 1.2 pH units different that the pH of the buffered suspension. The buffered suspension can be prepared by mixing a stock solution with the solid buffer particulate and titrating. A method of preforming a pH sensitive process includes drawing the buffered suspension from a reservoir, filtering the solid buffer particulate from the buffered suspension, and applying the filtered solution to a sensor.

    SEQUENCING DEVICE
    24.
    发明申请
    SEQUENCING DEVICE 审中-公开

    公开(公告)号：US20190264274A1

    公开(公告)日：2019-08-29

    申请号：US16289508

    申请日：2019-02-28

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Jonathan Schultz ,  Todd Roswech ,  Jon A. Hoshizaki ,  Albert L. Carrillo ,  James A, Ball

    IPC: C12Q1/6869 ,  B01F5/06 ,  B01F11/00 ,  B01L3/00 ,  G01N35/10 ,  C12Q1/6806 ,  B01F1/00 ,  G01N27/414

    Abstract: A method of preparing reagents includes inserting a cartridge into an instrument. The cartridge includes a plurality of reagent enclosures disposed in a cavity of the cartridge and exposing a port to an exterior of the cartridge. Each reagent enclosure includes a reagent container including a reagent and an internal cavity defining a compressible volume, an opening defined through the reagent container to the internal cavity. The method further includes connecting a plurality of fluid ports to the openings of the plurality of reagent enclosures; applying a solution through the fluid ports to at least partially fill the plurality of reagent enclosures; and cycling a pressure of the cavity, whereby for each of the reagent enclosures, during increasing pressure, the solution enters the internal cavity of the reagent container, combines with the reagent, and compresses the compressible volume, and during decreasing pressure, the compressible volume decreases and the reagent is ejected through the opening.

    PINCH FLOW REGULATOR
    26.
    发明申请
    PINCH FLOW REGULATOR 审中-公开

    公开(公告)号：US20170191571A1

    公开(公告)日：2017-07-06

    申请号：US15407333

    申请日：2017-01-17

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Jonathan Schultz

    IPC: F16K7/07 ,  G05D16/06 ,  B01L3/00

    CPC classification number: F16K7/07 ,  B01L3/567 ,  B01L2200/06 ,  C12Q1/6869 ,  F16K7/04 ,  G05D16/0638 ,  G05D16/185

    Abstract: A valve for regulating fluid flow includes a housing base defining a lower cavity and comprising a pinch structure within the lower cavity, a gas inlet providing external access to the lower cavity, a base fluid inlet, and a base fluid outlet. A housing cover defines an upper cavity and comprises a cover fluid inlet and a cover fluid outlet. The cover fluid inlet is in fluidic communication with the base fluid outlet between the upper cavity and the lower cavity, and the cover fluid outlet provides external access from the upper cavity. A diaphragm is disposed between the housing base and the housing cover. A pinch plate is disposed in the lower cavity and comprises a pinch point disposed opposite the pinch structure. A pinch tube is in fluidic communication between the base fluid inlet and the base fluid outlet in the lower cavity.

    FLUIDICS SYSTEM FOR SEQUENTIAL DELIVERY OF REAGENTS
    27.
    发明申请
    FLUIDICS SYSTEM FOR SEQUENTIAL DELIVERY OF REAGENTS 审中-公开
    用于顺序递送试剂的流体系统

    公开(公告)号：US20170056881A1

    公开(公告)日：2017-03-02

    申请号：US15348907

    申请日：2016-11-10

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Jonathan Schultz ,  David Marran

    IPC: B01L3/00 ,  G01N27/447

    CPC classification number: B01L3/502715 ,  B01J2219/00389 ,  B01J2219/00394 ,  B01L3/502 ,  B01L3/50273 ,  B01L3/502738 ,  B01L3/502746 ,  B01L3/502769 ,  B01L3/502776 ,  B01L3/52 ,  B01L2200/16 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/0874 ,  B01L2300/0877 ,  B01L2400/0487 ,  C12Q1/6869 ,  G01N27/447 ,  G01N35/1002 ,  G01N35/1097 ,  Y10T137/0318 ,  Y10T137/0424 ,  Y10T137/85938 ,  C12Q2565/629 ,  C12Q2527/119

    Abstract: The invention provides a passive fluidics circuit for directing different fluids to a common volume, such as a reaction chamber or flow cell, without intermixing or cross contamination. The direction and rate of flow through junctions, nodes and passages of the fluidics circuit are controlled by the states of upstream valves (e.g. opened or closed), differential fluid pressures at circuit inlets or upstream reservoirs, flow path resistances, and the like. Free diffusion or leakage of fluids from unselected inlets into the common outlet or other inlets at junctions or nodes is prevented by the flow of the selected inlet fluid, a portion of which sweeps by the inlets of unselected fluids and exits the fluidics circuit by waste ports, thereby creating a barrier against undesired intermixing with the outlet flow through leakage or diffusion. The invention is particularly advantageous in apparatus for performing sensitive multistep reactions, such as pH-based DNA sequencing reactions.

    Abstract translation: 本发明提供了一种用于将不同流体引导到公共体积（例如反应室或流动池）的无源流体回路，而不会混合或交叉污染。 通过流体回路的连接点，节点和通道的流动方向和流速由上游阀（例如打开或关闭）的状态，电路入口或上游储存器处的差分流体压力，流动阻力等来控制。 通过所选择的入口流体的流动防止流体从未选择的入口到共同的出口或其他入口处的自由扩散或泄漏，其一部分由未选择的流体的入口扫过并且通过废物端口离开流体回路 ，从而产生阻止与出口流通过泄漏或扩散的不期望的混合的屏障。 本发明在用于进行灵敏多步反应（例如基于pH的DNA测序反应）的装置中特别有利。

    Pinch flow regulator
    28.
    发明授权
    Pinch flow regulator 有权
    夹紧流量调节器

    公开(公告)号：US09579654B2

    公开(公告)日：2017-02-28

    申请号：US15162876

    申请日：2016-05-24

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Jonathan Schultz

    IPC: B01L99/00 ,  B01L3/00 ,  C12Q1/68 ,  F16K7/07 ,  G05D16/06 ,  G05D16/18 ,  F16K7/04

    CPC classification number: F16K7/07 ,  B01L3/567 ,  B01L2200/06 ,  C12Q1/6869 ,  F16K7/04 ,  G05D16/0638 ,  G05D16/185

    Abstract: A valve for regulating fluid flow includes a housing base defining a lower cavity and comprising a pinch structure within the lower cavity, a gas inlet providing external access to the lower cavity, a base fluid inlet, and a base fluid outlet. A housing cover defines an upper cavity and comprises a cover fluid inlet and a cover fluid outlet. The cover fluid inlet is in fluidic communication with the base fluid outlet between the upper cavity and the lower cavity, and the cover fluid outlet provides external access from the upper cavity. A diaphragm is disposed between the housing base and the housing cover. A pinch plate is disposed in the lower cavity and comprises a pinch point disposed opposite the pinch structure. A pinch tube is in fluidic communication between the base fluid inlet and the base fluid outlet in the lower cavity.

    Abstract translation: 用于调节流体流动的阀包括限定下腔的壳体底座，并且在下腔体内包括夹紧结构，提供对下腔室的外部通路的气体入口，基础流体入口和基础流体出口。 壳体盖限定上腔，并且包括盖流体入口和盖流体出口。 覆盖流体入口与上腔和下腔之间的基础流体出口流体连通，并且盖流体出口提供从上腔体的外部通路。 隔膜设置在壳体基座和壳体盖之间。 压板设置在下腔体中，并且包括与夹紧结构相对设置的夹点。 夹管在底部流体入口和下腔体中的基础流体出口之间流体连通。

    Alternative nucleotide flows in sequencing-by-synthesis methods
    30.
    发明授权
    Alternative nucleotide flows in sequencing-by-synthesis methods 有权
    按顺序排序的方法中的替代核苷酸流

    公开(公告)号：US09416413B2

    公开(公告)日：2016-08-16

    申请号：US13859673

    申请日：2013-04-09

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Jonathan Schultz ,  John Davidson

    IPC: C12Q1/68 ,  B01L3/00

    CPC classification number: C12Q1/6869 ,  B01L3/5027 ,  C12Q1/6874 ,  C12Q2537/155 ,  C12Q2563/149 ,  C12Q2565/301 ,  C12Q2565/629

    Abstract: A method for sequencing a polynucleotide strand by using sequencing-by-synthesis techniques. To address the problem of incomplete extension (IE) and/or carry forward (CF) errors that can occur in sequencing-by-synthesis reactions, an alternative flow ordering of dNTPs is used. In contrast to conventional flow orderings, the dNTPs are flowed in an ordering that is not a continuous repeat of an ordering of the four different dNTPs. This alternate flow ordering may reduce the loss of phasic synchrony in the population of template polynucleotide strands that result from IE and/or CF errors.

    Abstract translation: 通过使用按顺序合成技术测序多核苷酸链的方法。 为了解决在逐个合成反应中可能发生的不完全扩展（IE）和/或结转（CF）错误的问题，使用了dNTP的替代流顺序。 与常规流顺序相反，dNTP以不是四种不同dNTP的顺序的连续重复的顺序流动。 这种交替流顺序可以减少由IE和/或CF错误导致的模板多核苷酸链群体中的相位同步损失。

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