中科微点-全球专利检索

  1. Login
  2. Sign Up

Search Results

48 records (took 0.035 seconds)

    DETECTION AND QUANTIFICATION OF NUCLEIC ACID MOLECULES ASSOCIATED WITH A SURFACE
    26.
    发明申请
    DETECTION AND QUANTIFICATION OF NUCLEIC ACID MOLECULES ASSOCIATED WITH A SURFACE 审中-公开

    公开(公告)号：US20180363037A1

    公开(公告)日：2018-12-20

    申请号：US15781899

    申请日：2016-12-07

    Applicant: LIFE TECHNOLOGIES CORPORATION ,  THERMO FISHER SCIENTIFIC GENEART GMBH

    Inventor： Korbinian HEIL ,  Frank NOTKA ,  Kyle GEE ,  Jason DALLWIG ,  Phillip KUHN ,  Schuyler CORRY

    IPC: C12Q1/6834

    Abstract: This disclosure relates to methods, compositions, and related apparatuses for detection and quantification of nucleic acid molecules associated with a solid surface. Methods can include detecting or quantifying at least one nucleic acid molecule associated with a surface by contacting the at least one nucleic acid molecule with a fluorophore, wherein the fluorophore emits increased fluorescence at a wavelength when in contact with nucleic acid molecule, and detecting or measuring fluorescence from the fluorophore at the wavelength. Compositions can comprise a fluorophore and a nucleic acid molecule, wherein the nucleic acid molecule is associated with a surface, and the fluorophore has the property of emitting increased fluorescence at a wavelength when in contact with a nucleic acid molecule.

    COMPOSITION AND METHOD FOR MEASURING THALLIUM INFLUX AND EFFLUX
    29.
    发明申请
    COMPOSITION AND METHOD FOR MEASURING THALLIUM INFLUX AND EFFLUX 审中-公开
    用于测量THILIUM INFLUX和EFFLUX的组合物和方法

    公开(公告)号：US20170067918A1

    公开(公告)日：2017-03-09

    申请号：US15269326

    申请日：2016-09-19

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Daniel BEACHAM ,  Kyle GEE

    IPC: G01N33/84 ,  G01N33/68

    CPC classification number: G01N33/84 ,  C12Q1/02 ,  G01N33/6872 ,  G01N2333/705

    Abstract: The present invention relates to methods for detecting the activity of an ion channel in a cell. The methods comprise providing a loading buffer solution to a cell that has an ion channel. The loading buffer comprises at least one thallium indicator (e.g., an environmentally sensitive, luminescent dye) and a physiological concentration of chloride ions. The methods further comprise providing a stimulus buffer to the cell, wherein the stimulus buffer comprises thallium (e.g., thallium ions). Providing the stimulus buffer causes thallium influx into the cell through the ion channel. After providing the stimulus buffer, the luminescence (e.g., fluorescence) of the dye in the cell is detected. The luminescence of the dye can change in the presence or absence of thallium. The methods may be used to measure influx or efflux of thallium through an ion channel.

    Abstract translation: 本发明涉及用于检测细胞中离子通道的活性的方法。 所述方法包括向具有离子通道的电池提供加载缓冲溶液。 加载缓冲液包含至少一种铊指示剂（例如环境敏感的发光染料）和氯离子的生理浓度。 所述方法还包括向细胞提供刺激缓冲液，其中刺激缓冲液包含铊（例如铊离子）。 提供刺激缓冲液使铊通过离子通道流入细胞。 在提供刺激缓冲液之后，检测细胞中染料的发光（例如荧光）。 在存在或不存在铊的情况下，染料的发光可以改变。 该方法可用于测量铊通过离子通道的流入或流出。

Patent Agency Ranking