公开(公告)号：US20060228749A1

公开(公告)日：2006-10-12

申请号：US11448636

申请日：2006-06-07

申请人： Xiaobo Wang ,  Lei Wu ,  Jing Cheng ,  Weiping Yang ,  Junquan Xu

发明人： Xiaobo Wang ,  Lei Wu ,  Jing Cheng ,  Weiping Yang ,  Junquan Xu

IPC分类号： C40B40/08 ,  C40B40/10 ,  C12Q1/68 ,  G01N33/551

CPC分类号： G01N27/745 ,  B01J2219/00527 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00612 ,  B01J2219/00617 ,  B01J2219/00619 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/00639 ,  B01J2219/00653 ,  B01J2219/00659 ,  B01J2219/00702 ,  B01L3/5027 ,  C12Q1/6837 ,  C12Q1/6874 ,  G01N33/54326 ,  G01N33/5438 ,  C12Q2565/629

摘要： This invention relates generally to the field of moiety or molecule manipulation in a chip format. In particular, the invention provides a method for manipulating a moiety in a microfluidic application, which method comprises: a) coupling a moiety to be manipulated onto surface of a binding partner of said moiety to form a moiety-binding partner complex; and b) manipulating said moiety-binding partner complex with a physical force in a chip format, wherein said manipulation is effected through a combination of a structure that is external to said chip and a structure that is built-in in said chip, thereby said moiety is manipulated.

摘要翻译： 本发明一般涉及芯片格式的部分或分子操作领域。 特别地，本发明提供了一种在微流体应用中操作部分的方法，该方法包括：a）将待操作的部分偶联到所述部分的结合配偶体的表面上以形成部分结合配偶体复合物; 和b）以芯片格式的物理力操纵所述部分结合配偶体复合物，其中所述操作通过所述芯片外部的结构和内置在所述芯片中的结构的组合来实现，由此所述 部分被操纵。

公开(公告)号：US08206903B2

公开(公告)日：2012-06-26

申请号：US11286882

申请日：2005-11-23

申请人： Xiaobo Wang ,  Yama A. Abassi ,  Josephine Atienza ,  Xiao Xu ,  Junquan Xu

发明人： Xiaobo Wang ,  Yama A. Abassi ,  Josephine Atienza ,  Xiao Xu ,  Junquan Xu

IPC分类号： C12Q1/68

CPC分类号： C12N15/87 ,  C12M35/02 ,  G01N15/12 ,  G01N33/48728

摘要： The present invention includes devices and methods for transfecting a cell or cell population and dynamic monitoring of cellular events. A variety of microelectronic devices are provide that incorporate functions such as electroporation, modulation of a transmembrane potential and dynamic monitoring of cellular functions and mechanisms.

摘要翻译： 本发明包括用于转染细胞或细胞群的装置和方法以及细胞事件的动态监测。 提供了多种微电子器件，其包括诸如电穿孔，跨膜电位的调节和细胞功能和机制的动态监测的功能。

公开(公告)号：US08921041B2

公开(公告)日：2014-12-30

申请号：US13474355

申请日：2012-05-17

申请人： Xiaobo Wang ,  Yama A. Abassi ,  Josephine Atienza ,  Xiao Xu ,  Junquan Xu

发明人： Xiaobo Wang ,  Yama A. Abassi ,  Josephine Atienza ,  Xiao Xu ,  Junquan Xu

IPC分类号： C12Q1/68 ,  C12M1/42 ,  C12N15/87

CPC分类号： C12N15/87 ,  C12M35/02 ,  G01N15/12 ,  G01N33/48728

摘要： The present invention includes devices and methods for transfecting a cell or cell population and dynamic monitoring of cellular events. A variety of microelectronic devices are provide that incorporate functions such as electroporation, modulation of a transmembrane potential and dynamic monitoring of cellular functions and mechanisms.

摘要翻译： 本发明包括用于转染细胞或细胞群的装置和方法以及细胞事件的动态监测。 提供了多种微电子器件，其包括诸如电穿孔，跨膜电位的调节和细胞功能和机制的动态监测的功能。

公开(公告)号：US20060121446A1

公开(公告)日：2006-06-08

申请号：US11286882

申请日：2005-11-23

申请人： Yama Abassi ,  Xiaobo Wang ,  Josephine Atienza ,  Xiao Xu ,  Junquan Xu

发明人： Yama Abassi ,  Xiaobo Wang ,  Josephine Atienza ,  Xiao Xu ,  Junquan Xu

IPC分类号： C12Q1/00 ,  C12N15/86

CPC分类号： C12N15/87 ,  C12M35/02 ,  G01N15/12 ,  G01N33/48728

摘要： The present invention includes devices and methods for transfecting a cell or cell population and dynamic monitoring of cellular events. A variety of microelectronic devices are provide that incorporate functions such as electroporation, modulation of a transmembrane potential and dynamic monitoring of cellular functions and mechanisms.

摘要翻译： 本发明包括用于转染细胞或细胞群的装置和方法以及细胞事件的动态监测。 提供了多种微电子器件，其包括诸如电穿孔，跨膜电位的调节和细胞功能和机制的动态监测的功能。

公开(公告)号：US20120225424A1

公开(公告)日：2012-09-06

申请号：US13474355

申请日：2012-05-17

申请人： Yama A. Abassi ,  Xiaobo Wang ,  Josephine Atienza ,  Xiao Xu ,  Junquan Xu

发明人： Yama A. Abassi ,  Xiaobo Wang ,  Josephine Atienza ,  Xiao Xu ,  Junquan Xu

IPC分类号： G01N27/02 ,  G01N21/00 ,  C12M1/42 ,  G01N21/64

CPC分类号： C12N15/87 ,  C12M35/02 ,  G01N15/12 ,  G01N33/48728

摘要： The present invention includes devices and methods for transfecting a cell or cell population and dynamic monitoring of cellular events. A variety of microelectronic devices are provide that incorporate functions such as electroporation, modulation of a transmembrane potential and dynamic monitoring of cellular functions and mechanisms.

摘要翻译： 本发明包括用于转染细胞或细胞群的装置和方法以及细胞事件的动态监测。 提供了多种微电子器件，其包括诸如电穿孔，跨膜电位的调节和细胞功能和机制的动态监测的功能。

公开(公告)号：US06596143B1

公开(公告)日：2003-07-22

申请号：US09678263

申请日：2000-10-03

申请人： Xiao-Bo Wang ,  Weiping Yang ,  Junquan Xu ,  Jing Cheng ,  Lei Wu

发明人： Xiao-Bo Wang ,  Weiping Yang ,  Junquan Xu ,  Jing Cheng ,  Lei Wu

IPC分类号： G01N2726

CPC分类号： H02N11/006 ,  B01D57/02 ,  B03C5/028 ,  H02N1/004

摘要： A device acts as a particle switch to transport and/or re-direct microparticles which are in a fluid suspension. The switch comprises at least three structural branches and the branches may be connected at a common junction. Particles can be transported along the branches as a result of the forces generated along that branch. Particles are transported into or out of the particle switch via the ends of the branches. Particles can be switched from one branch into one of the other branches. Depending on the properties of the particles, the transportation mechanism may be traveling-wave-dielectrophoresis or traveling-wave-electrophoresis.

摘要翻译： 装置用作颗粒开关以运输和/或重新引导处于流体悬浮液中的微粒。 开关包括至少三个结构分支，并且分支可以在公共接头处连接。 由于沿该分支产生的力，颗粒可以沿着分支运输。 颗粒通过分支的末端被输送进出颗粒开关。 颗粒可以从一个分支切换到其他分支之一。 根据颗粒的性质，运输机制可以是行波介电电泳或行波电泳。

公开(公告)号：US06881314B1

公开(公告)日：2005-04-19

申请号：US09679023

申请日：2000-10-04

申请人： Xiao-Bo Wang ,  Jing Cheng ,  Lei Wu ,  Junquan Xu

发明人： Xiao-Bo Wang ,  Jing Cheng ,  Lei Wu ,  Junquan Xu

IPC分类号： B01J19/00 ,  B03C1/00 ,  B03C5/00 ,  B81B1/00 ,  C02F1/40 ,  C25B9/00 ,  C25B11/00 ,  C25B13/00 ,  G01N27/27

CPC分类号： B03C1/00 ,  B01D21/283 ,  B01J19/0093 ,  B01L2400/0415 ,  B01L2400/043 ,  B01L2400/0436 ,  B01L2400/0442 ,  B01L2400/0454 ,  B01L2400/0487 ,  B03C5/005 ,  G01N2030/0015

摘要： This invention relates generally to the field of field-flow-fractionation. In particular, the invention provides apparatuses and methods for the discrimination of matters utilizing acoustic force, or utilizing acoustic force with electrophoretic or dielectrophoretic force, in field flow fractionation.

摘要翻译： 本发明一般涉及现场流分馏领域。 特别地，本发明提供了用于区分利用声力或利用电泳或介电电泳力的声力的现场流分级的装置和方法。

公开(公告)号：US06630835B2

公开(公告)日：2003-10-07

申请号：US10216003

申请日：2002-08-07

申请人： Jing Cheng ,  Junquan Xu ,  Xiaoshan Zhu ,  Litian Liu ,  Xiao-Bo Wang ,  Lei Wu

发明人： Jing Cheng ,  Junquan Xu ,  Xiaoshan Zhu ,  Litian Liu ,  Xiao-Bo Wang ,  Lei Wu

IPC分类号： G01R2708

CPC分类号： G01N33/48707 ,  B01J2219/00497 ,  B01J2219/00527 ,  B01J2219/00529 ,  B01J2219/00587 ,  B01J2219/00596 ,  B01J2219/00653 ,  B01J2219/00659 ,  B01J2219/00707 ,  B01J2219/0072 ,  Y10S436/809

摘要： The present invention concerns a high throughput electrorotation chip having an array of electrorotation units and methods of use thereof. To make the high throughput electrorotation chip, a plurality of electrorotation units (EU) are fabricated on a substrate or support and each EU is capable of producing a rotating electric field upon the application of an appropriate electrical signal. Exemplary embodiments include a row-column configuration of EUs having four electrode elements realized through two conductive-layers. The electrode elements may be linear, concave, or convex. Thin plates having one or multiple holes are bound to high-throughput electrorotation chips to form assay chambers having one or multiple wells. Particles can be introduced to the wells and electrorotation measurements can be performed on the particles. The high throughput electrorotation chip and chamber may be used for cell-based screening for leading drug candidate molecules from a compound library, for high-throughput characterizing particle electric properties, and for high-throughput assaying molecular compositions of unknown solutions.

公开(公告)号：US06448794B1

公开(公告)日：2002-09-10

申请号：US09643362

申请日：2000-08-22

申请人： Jing Cheng ,  Junquan Xu ,  Xiaoshan Zhu ,  Litian Liu ,  Xiao-Bo Wang ,  Lei Wu

发明人： Jing Cheng ,  Junquan Xu ,  Xiaoshan Zhu ,  Litian Liu ,  Xiao-Bo Wang ,  Lei Wu

IPC分类号： G01R2708

CPC分类号： G01N33/48707 ,  B01J2219/00497 ,  B01J2219/00527 ,  B01J2219/00529 ,  B01J2219/00587 ,  B01J2219/00596 ,  B01J2219/00653 ,  B01J2219/00659 ,  B01J2219/00707 ,  B01J2219/0072 ,  Y10S436/806

摘要： The present invention concerns a high throughput electrorotation chip having an array of electrorotation units and methods of use thereof. To make the high throughput electrorotation chip, a plurality of electrorotation units (EU) are fabricated on a substrate or support and each EU is capable of producing a rotating electric field upon the application of an appropriate electrical signal. Exemplary embodiments include a row-column configuration of EUs having four electrode elements realized through two conductive-layers. The electrode elements may be linear, concave, or convex. Thin plates having one or multiple holes are bound to high-throughput electrorotation chips to form assay chambers having one or multiple wells. Particles can be introduced to the wells and electrorotation measurements can be performed on the particles. The high throughput electrorotation chip and chamber may be used for cell-based screening for leading drug candidate molecules from a compound library, for high-throughput characterizing particle electric properties, and for high-throughput assaying molecular compositions of unknown solutions.

摘要翻译： 本发明涉及一种具有电极单元阵列的高通量电选机芯及其使用方法。 为了制造高通量电选芯片，在基板或支撑件上制造多个电极单元（EU），并且每个EU在施加适当的电信号时能够产生旋转电场。 示例性实施例包括具有通过两个导电层实现的四个电极元件的EU行的列列配置。 电极元件可以是线性的，凹的或凸的。 具有一个或多个孔的薄板结合到高通量电旋转切片以形成具有一个或多个孔的测定室。 可以将颗粒引入孔中，并且可以对颗粒进行电转化测量。 高通量电刺激芯片和腔室可用于来自化合物文库的主要药物候选分子的基于细胞的筛选，用于高通量表征颗粒电性质，以及用于高通量测定未知溶液的分子组成。

公开(公告)号：US06355491B1

公开(公告)日：2002-03-12

申请号：US09399299

申请日：1999-09-17

申请人： Yuxiang Zhou ,  Litian Liu ,  Ken Chen ,  Depu Chen ,  Jia Wang ,  Zewen Liu ,  Zhimin Tan ,  Junquan Xu ,  Xiaoshan Zhu ,  Xuezhong He ,  Wenzhang Xie ,  Zhiming Li ,  Xiumei Liu

发明人： Yuxiang Zhou ,  Litian Liu ,  Ken Chen ,  Depu Chen ,  Jia Wang ,  Zewen Liu ,  Zhimin Tan ,  Junquan Xu ,  Xiaoshan Zhu ,  Xuezhong He ,  Wenzhang Xie ,  Zhiming Li ,  Xiumei Liu

IPC分类号： G01N33543

CPC分类号： B01L3/502761 ,  B01J19/0046 ,  B01J2219/00317 ,  B01J2219/00468 ,  B01J2219/005 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00614 ,  B01J2219/00626 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00639 ,  B01J2219/00648 ,  B01J2219/00653 ,  B01J2219/00659 ,  B01J2219/00722 ,  B01L3/5025 ,  B01L2200/0647 ,  B01L2200/0668 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0877 ,  B01L2300/089 ,  B01L2400/043 ,  C40B40/06 ,  C40B60/14 ,  G01N35/0098 ,  Y10S436/806

摘要： This invention provides electromagnetic chips and electromagnetic biochips having arrays of individually addressable micro-electromagnetic units, as well as methods of utilizing these chips for directed manipulation of micro-particles and micro-structures such as biomolecules and chemical reagents. An electromagnetic biochip comprises an individually addressable micro-electromagnetic unit chip with ligand molecules immobilized on its surface. By controlling the electromagnetic field at each unit of the array and combining this control with magnetic modification of biomolecules, these chips can be used for directed manipulation, synthesis and release of biomolecules in order to increase sensitivity of biochemical or chemical analysis and reduce assay time. Other advantages with these chips include minimized damages to biological molecules and increased reproducibility of assay results.

摘要翻译： 本发明提供具有独立寻址的微电磁单元阵列的电磁芯片和电磁生物芯片，以及利用这些芯片来定向操纵微粒和微结构如生物分子和化学试剂的方法。 电磁生物芯片包括具有固定在其表面上的配体分子的单独可寻址的微电磁单元芯片。 通过控制阵列的每个单元的电磁场并将该控制与生物分子的磁性修饰相组合，这些芯片可用于生物分子的定向操作，合成和释放，以提高生物化学或化学分析的灵敏度并减少测定时间。 这些芯片的其他优点包括对生物分子的最小化损害和提高测定结果的再现性。