公开(公告)号：US20120028313A1

公开(公告)日：2012-02-02

申请号：US12897745

申请日：2010-10-04

申请人： Yoshihide Hayashizaki

发明人： Yoshihide Hayashizaki

IPC分类号： C12N15/66 ,  C12P19/34

CPC分类号： C12P19/34 ,  C12N15/1096 ,  C12N15/66 ,  C40B40/00

摘要： The present invention relates to a linker or population of linkers that include an oligonucleotide fixed portion and an oligonucleotide variable portion represented by formula (N)n, wherein N is A, C, G, T or U, or their derivatives, and n is an integer equal to or higher than 1. A linker-polynucleotide or a population of linker-polynucleotides of the invention may be constituted by said linker or population of linkers and a target first strand polynucleotide bound to said linker. The invention also encompasses a method of preparing said linker or population of linkers and a method of preparing a linker-polynucleotide using said linker or population of linkers. The linkers or polynucleotide-linkers of the invention can be used in a method of preparing a cDNA library.

摘要翻译： 本发明涉及包含寡核苷酸固定部分和由式（N）n表示的寡核苷酸可变部分的接头或接头群体，其中N为A，C，G，T或U，或其衍生物，n为 本发明的接头多核苷酸或接头多聚核苷酸群体可以由所述连接体或接头群体和与所述接头结合的靶第一链多核苷酸构成。 本发明还包括制备所述接头或接头群体的方法，以及使用所述接头或接头群体制备接头 - 多核苷酸的方法。 本发明的接头或多核苷酸接头可用于制备cDNA文库的方法。

公开(公告)号：US08067162B2

公开(公告)日：2011-11-29

申请号：US12075198

申请日：2008-03-10

申请人： Yoshihide Hayashizaki ,  Akimitsu Okamoto ,  Alexander Lezhava ,  Yasushi Kogo

发明人： Yoshihide Hayashizaki ,  Akimitsu Okamoto ,  Alexander Lezhava ,  Yasushi Kogo

IPC分类号： C12Q1/68 ,  C12P19/38 ,  C07H21/02

CPC分类号： C12Q1/6853 ,  C12Q2525/117 ,  C12Q2563/107 ,  C12Q2565/101

摘要： The present invention provides a primer that effectively can detect, for example, the double helix structure of a nucleic acid. The primer is a labeled nucleic acid containing at least one structure represented by the following formula (16), where B is an atomic group having a nucleobase skeleton, E is an atomic group having a deoxyribose skeleton, a ribose skeleton, or a structure derived from either one of them, or an atomic group having a peptide structure or a peptoid structure, and Z11 and Z12 are each an atomic group that exhibits fluorescence and are identical to or different from each other.

摘要翻译： 本发明提供了有效地检测例如核酸的双螺旋结构的引物。 引物是含有至少一个下式（16）表示的结构的标记核酸，其中B是具有核碱基骨架的原子团，E是具有脱氧核糖骨架的原子团，核糖骨架或衍生的结构 或者具有肽结构或拟肽结构的原子团，Z 11和Z 12各自为表现出荧光且彼此相同或不同的原子团。

公开(公告)号：US07803579B2

公开(公告)日：2010-09-28

申请号：US10532975

申请日：2003-10-29

申请人： Yasumasa Mitani ,  Akio Yamane ,  Yuko Shibata ,  Yoshihide Hayashizaki

发明人： Yasumasa Mitani ,  Akio Yamane ,  Yuko Shibata ,  Yoshihide Hayashizaki

IPC分类号： C12P19/34 ,  C12Q1/68 ,  C07H21/04

CPC分类号： C12Q1/6844 ,  C12Q2525/301

摘要： The present invention relates to a process for synthesizing or amplifying efficiently a nucleic acid comprising a target nucleic acid sequence. The process involves providing a primer comprising in its 3′-end portion a sequence (Ac′) which hybridizes a sequence (A) in the 3′-end portion of the target nucleic acid sequence, and in the 5′-side of the sequence (Ac′) a sequence (B′) which hybridizes the complementary sequence (Bc) of a sequence (B) positioned in the 5′-side of the sequence (A) on the target nucleic acid sequence, wherein {X−(Y−Y′)}/X is in the range of −1.00 to 1.00, in which X denotes the number of bases in the sequence (Ac′), Y denotes the number of bases in the region flanked by the sequences (A) and (B) in the target nucleic acid sequence, and Y′ denotes the number of bases in an intervening sequence between the sequences (Ac′) and (B′) (Y′ may be zero).

摘要翻译： 本发明涉及有效地合成或扩增包含靶核酸序列的核酸的方法。 该方法包括提供一种引物，其在其3'末端部分包含与目标核酸序列的3'末端部分的序列（A）和5'侧杂交的序列（Ac'） 序列（Ac'）与位于靶核酸序列上的序列（A）的5'侧的序列（B）的互补序列（Bc）杂交的序列（B'），其中{X（ Y-Y'）} / X在-1.00至1.00的范围内，其中X表示序列中的碱基数（Ac'），Y表示侧翼为序列（A）的区域中的碱基数， 和（B），Y'表示序列（Ac'）和（B'）之间的间隔序列中的碱基数（Y'可以为零）。

公开(公告)号：US20100047862A1

公开(公告)日：2010-02-25

申请号：US12083695

申请日：2006-10-20

申请人： Yoshihide Hayashizaki ,  Masayoshi Itoh ,  Yoshimi Benno ,  Alexander Lezhava

发明人： Yoshihide Hayashizaki ,  Masayoshi Itoh ,  Yoshimi Benno ,  Alexander Lezhava

IPC分类号： C12P21/00 ,  C12N9/12 ,  C07H21/00 ,  C12N15/63 ,  C12N1/00 ,  C12P19/34

CPC分类号： C12N9/1276 ,  C12N9/1252

摘要： This invention provides a novel DNA polymerase obtained from Bacillus smithii JCM9076, which has novel features in terms of, for example, optimal reaction conditions (e.g., optimal temperature) and enzyme activity. More particularly, a novel DNA polymerase is a pol I type DNA polymerase, which is any of proteins (a) to (f) below and has DNA polymerase activity: (a) a protein comprising the amino acid sequence as shown in SEQ ID NO: 7; (b) a protein consisting of an amino acid sequence derived from the amino acid sequence as shown in SEQ ID NO: 7 by deletion, substitution, or addition of one or several amino acid residues; (c) a protein consisting of the amino acid sequence as shown in SEQ ID NO: 9; and (d) a protein consisting of an amino acid sequence derived from the amino acid sequence as shown in SEQ ID NO: 9 by deletion, substitution, or addition of one or several amino acid residues.

摘要翻译： 本发明提供了一种从芽孢杆菌JCM9076获得的新型DNA聚合酶，其在例如最佳反应条件（例如最佳温度）和酶活性方面具有新特征。 更具体地，新型DNA聚合酶是pol I型DNA聚合酶，其是以下蛋白质（a）至（f）中的任一种，并且具有DNA聚合酶活性：（a）包含SEQ ID NO所示的氨基酸序列的蛋白质 ：7; （b）通过缺失，取代或添加一个或几个氨基酸残基的由SEQ ID NO：7所示的氨基酸序列衍生的氨基酸序列组成的蛋白质; （c）由SEQ ID NO：9所示的氨基酸序列组成的蛋白质; 和（d）通过缺失，取代或添加一个或几个氨基酸残基，由SEQ ID NO：9所示的氨基酸序列衍生的氨基酸序列组成的蛋白质。

公开(公告)号：US07531076B2

公开(公告)日：2009-05-12

申请号：US11326368

申请日：2006-01-06

申请人： Yoshihide Hayashizaki ,  Rintaro Yamamoto

发明人： Yoshihide Hayashizaki ,  Rintaro Yamamoto

IPC分类号： G01N27/453

CPC分类号： G01N27/44782 ,  Y10S83/95 ,  Y10T29/5148 ,  Y10T29/53391 ,  Y10T83/0596

摘要： Capillary columns (102) pass through and are inserted in a rubber plate (14), held and fixed by elastic force of rubber, and two-dimensionally arranged on a sample injection side. it fixes the capillary columns (102) arranged on a plane in close contact by holding the same with a holder plate (6a) from below and with a rubber plate (16) from above on a detection side. In order to press the capillary columns (102) against the holder plate 6a and fix the same with the rubber plate (16), a holder plate (6b) fixing the rubber plate (16) to the holder plate (6a) on both sides of the arrangement of the capillary columns (102) is provided.

摘要翻译： 毛细管柱（102）穿过并被插入橡胶板（14）中，橡胶板通过橡胶的弹力保持和固定，并且二维地布置在样品注射侧。 它通过将保持板（6a）从下方夹持而在安装在平面上的毛细管柱（102）上固定，并在检测侧从橡胶板（16）上方固定。 为了将毛细管柱（102）压靠在保持板6a上并将其固定在橡胶板（16）上，将橡胶板（16）固定在两侧的保持板（6a）上的保持板（6b） 提供毛细管柱（102）的布置。

公开(公告)号：US20090042197A1

公开(公告)日：2009-02-12

申请号：US12094896

申请日：2006-11-21

申请人： Yoshihide Hayashizaki ,  Yasumasa Mitani ,  Yuki Kawai

发明人： Yoshihide Hayashizaki ,  Yasumasa Mitani ,  Yuki Kawai

IPC分类号： C12Q1/68 ,  C12P19/34

CPC分类号： C12Q1/6844 ,  C12Q1/6865 ,  C12Q2527/125 ,  C12Q2525/301

摘要： Problem to be Solved There is provided a method for detecting and/or amplifying a nucleic acid contained in a biological sample such as blood or cells conveniently, rapidly, and effectively.Solution There is provided a method for detecting a nucleic acid contained in a sample, comprising the step of adding at least one substance selected from the group consisting of polyphenols, polyhydric alcohols, sugar acids, sugar alcohols, and hydrophilic biodegradable polymers to a sample, the step of complementarily binding an oligonucleotide complementary to a part of the nucleic acid sequence of a nucleic acid to be detected to a part of the nucleic acid sequence, and the step of detecting the nucleic acid to be detected.

摘要翻译： 待解决的问题提供了一种方便，快速，有效地检测和/或扩增生物样品如血液或细胞中的核酸的方法。 溶液提供了一种检测样品中所含的核酸的方法，其包括将至少一种选自多酚，多元醇，糖酸，糖醇和亲水性可生物降解聚合物的物质加入到样品中的步骤， 将与要检测的核酸的一部分核酸序列互补的寡核苷酸互补结合到核酸序列的一部分的步骤和检测待检测的核酸的步骤。

公开(公告)号：US20080227104A1

公开(公告)日：2008-09-18

申请号：US12075198

申请日：2008-03-10

申请人： Yoshihide Hayashizaki ,  Akimitsu Okamoto ,  Alexander Lezhava ,  Yasushi Kogo

发明人： Yoshihide Hayashizaki ,  Akimitsu Okamoto ,  Alexander Lezhava ,  Yasushi Kogo

IPC分类号： C12Q1/68 ,  C07H21/00 ,  C12P19/34 ,  C12N9/10

CPC分类号： C12Q1/6853 ,  C12Q2525/117 ,  C12Q2563/107 ,  C12Q2565/101

摘要： The present invention provides a primer that effectively can detect, for example, the double helix structure of a nucleic acid. The primer is a labeled nucleic acid containing at least one structure represented by the following formula (16), where B is an atomic group having a nucleobase skeleton, E is an atomic group having a deoxyribose skeleton, a ribose skeleton, or a structure derived from either one of them, or an atomic group having a peptide structure or a peptoid structure, and Z11 and Z12 are each an atomic group that exhibits fluorescence and are identical to or different from each other.

摘要翻译： 本发明提供了有效地检测例如核酸的双螺旋结构的引物。 引物是含有至少一个下式（16）表示的结构的标记核酸，其中B是具有核碱基骨架的原子团，E是具有脱氧核糖骨架的原子团，核糖骨架或衍生的结构 或者具有肽结构或拟肽结构的原子团，Z 11和Z 12都是表现出荧光且相同的原子团 彼此之间或彼此不同。

公开(公告)号：US20060123505A1

公开(公告)日：2006-06-08

申请号：US10449902

申请日：2003-05-29

申请人： Shoshi Kikuchi ,  Naoki Kishimoto ,  Kouji Satoh ,  Toshifumi Nagata ,  Nobuyuki Kawagashira ,  Junshi Yazaki ,  Masahiro Ishikawa ,  Koji Doi ,  Jun Kawai ,  Yoshihide Hayashizaki ,  Yasuhiro Otomo ,  Kenichi Matsubara ,  Kazuo Murakami

发明人： Shoshi Kikuchi ,  Naoki Kishimoto ,  Kouji Satoh ,  Toshifumi Nagata ,  Nobuyuki Kawagashira ,  Junshi Yazaki ,  Masahiro Ishikawa ,  Koji Doi ,  Jun Kawai ,  Yoshihide Hayashizaki ,  Yasuhiro Otomo ,  Kenichi Matsubara ,  Kazuo Murakami

IPC分类号： A01H5/00 ,  G06F19/00 ,  C07H21/04 ,  A01H1/00 ,  C07K14/415 ,  C07K16/18

CPC分类号： C07K14/415

摘要： Full-length cDNAs of plants and their uses are provided. Source plants are preferably monocot plants, more preferably poaceous plants, and most preferably rice. Vectors carrying said cDNAs and transformants containing said cDNAs or said vectors, transgenic plants containing said transformants, polypeptides encoded by said cDNAs are also provided. The full-length cDNA clones play important roles in the annotation of correct gene coding region, determination of exons and introns, comprehensive expression analysis on the transcription level and proteome analysis. Furthermore, full-length cDNA clones are industrially useful in producing plants having different properties from those of the wild type due to the inhibition of expression and functional suppression in plant bodies.

摘要翻译： 提供植物的全长cDNA及其用途。 源植物优选为单子叶植物，更优选为植物，最优选为稻。 还提供了携带所述cDNAs和含有所述cDNA或所述载体的转化体的载体，含有所述转化体的转基因植物，由所述cDNA编码的多肽。 全长cDNA克隆在正确的基因编码区域的注释，外显子和内含子的测定，转录水平的综合表达分析和蛋白质组分析中起重要作用。 此外，全长cDNA克隆在工业上可用于生产与野生型具有不同性质的植物，这是由于植物体中表达和功能抑制的抑制。

公开(公告)号：US20050032061A1

公开(公告)日：2005-02-10

申请号：US10494354

申请日：2002-11-05

申请人： Yoshihide Hayashizaki

发明人： Yoshihide Hayashizaki

IPC分类号： C12N15/09 ,  B01J19/00 ,  B01L3/00 ,  C12M1/00 ,  C12Q1/68 ,  C40B40/06 ,  C40B40/10 ,  C40B40/12 ,  C40B60/14 ,  C40B70/00 ,  G01N35/00 ,  C12M1/34

CPC分类号： B01L3/5055 ,  B01J19/0046 ,  B01J2219/00373 ,  B01J2219/00378 ,  B01J2219/00385 ,  B01J2219/00387 ,  B01J2219/00497 ,  B01J2219/00527 ,  B01J2219/00542 ,  B01J2219/00547 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00637 ,  B01J2219/00641 ,  B01J2219/00644 ,  B01J2219/00657 ,  B01J2219/00659 ,  B01J2219/00677 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/00729 ,  B01J2219/00731 ,  B01L9/56 ,  B01L2300/069 ,  C12Q1/6806 ,  C40B40/06 ,  C40B40/10 ,  C40B40/12 ,  C40B60/14 ,  C40B70/00 ,  G01N35/00009 ,  C12Q2565/518

摘要： A method for storing and/or delivering an oligomer and/or polymer applied on at least one support, comprising the steps of: (a) applying at least one oligomer and/or polymer on at least one support; (b) folding or rolling the support; and (c) storing and/or delivering the support of step (b) is provided. Also, a support having at least one oligomer and/or polymer applied thereon, which is in the form of a folded or rolled sheet, a loose-leaf sheet or a card and a water-soluble support having at least one oligomer and/or polymer applied thereon are provided. According to the present invention, molecular substances (oligomers and/or polymers) can be stored and delivered in a simple system. This system can preserve the substances without contamination and degradation.

摘要翻译： 一种用于储存和/或递送施用在至少一种载体上的低聚物和/或聚合物的方法，包括以下步骤：（a）在至少一种载体上施用至少一种低聚物和/或聚合物; （b）折叠或滚动支架; 并提供（c）存储和/或传递步骤（b）的支持。 此外，其上涂覆有至少一种低聚物和/或聚合物的载体，其为折叠或卷状片材，活页片或卡片的形式，以及具有至少一种低聚物和/或 提供其上施加的聚合物。 根据本发明，分子物质（低聚物和/或聚合物）可以在简单的系统中储存和输送。 该系统可以保护物质而不会污染和降解。

公开(公告)号：US06605257B1

公开(公告)日：2003-08-12

申请号：US09634875

申请日：2000-08-07

申请人： Touji Nakazawa ,  Akihiro Iimura ,  Hideo Tashiro ,  Tokuji Kitsunai ,  Yoshihide Hayashizaki

发明人： Touji Nakazawa ,  Akihiro Iimura ,  Hideo Tashiro ,  Tokuji Kitsunai ,  Yoshihide Hayashizaki

IPC分类号： B01L302

CPC分类号： B01L3/0251 ,  B01J19/0046 ,  B01J2219/00364 ,  B01J2219/00369 ,  B01J2219/00376 ,  B01J2219/00387 ,  B01J2219/00527 ,  B01J2219/00533 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00608 ,  B01J2219/00659 ,  B01J2219/00677 ,  B01J2219/00686 ,  B01J2219/00689 ,  B01J2219/00691 ,  B01J2219/00722 ,  B01L3/0244 ,  B01L3/0255 ,  B82Y30/00 ,  C40B60/14 ,  G01N2035/1037 ,  Y10T436/2575

摘要： A present micro array manufacturing apparatus includes a substrate placement portion 11 on which a plurality of substrates can be arranged, a tighter plate 12 for storing a solution including a living body sample, a solution storing member 52 for taking in the solution from the tighter plate 12 and storing the solution therein, and a needle 51 which can be inserted into the solution storing member 52 to allow the solution stored in the solution storing member 52 to adhere onto each of the substrates by a given quantity, wherein the solution storing member 52 and needle 51 are moved in directions where they approach and part away from the substrates to thereby form spots on the substrates. And, the individual operations of the solution storing member 52 and needle 51 are carried out by single drive means 32.

摘要翻译： 本发明的微阵列制造装置包括：基板配置部11，能够配置多个基板的基板配置部11;用于存储包含生物体样品的溶液的更紧密的板12;从更紧的板取出溶液的溶液存储部件52 12，将溶液保存在其中，将针51插入到溶液收容部件52中，使存储在溶液储存部件52中的溶液以规定量附着在各基板上，其中，溶液收容部件52 并且针51在它们接近并远离基板的方向上移动，从而在基板上形成点。 并且，溶液储存构件52和针51的各个操作由单个驱动装置32执行。