公开(公告)号：US09896486B2

公开(公告)日：2018-02-20

申请号：US14903837

申请日：2014-07-08

Applicant: GE Healthcare BioProcess R&D AB

Inventor： Gustav Rodrigo ,  Mats Ander ,  Tomas Bjorkman ,  Goran Bauren

IPC: C07K14/31 ,  C07K1/22 ,  B01D15/38 ,  B01J20/286 ,  B01J20/32 ,  C07K14/745 ,  C07K16/00

CPC classification number: C07K14/31 ,  B01D15/3809 ,  B01J20/286 ,  B01J20/3204 ,  B01J20/3212 ,  B01J20/3219 ,  B01J20/3274 ,  C07K1/22 ,  C07K14/745 ,  C07K16/00

Abstract: A polypeptide with improved alkaline stability, which polypeptide comprises a mutant of a B or C domain of Staphylococcus Protein A, as specified by SEQ ID NO 1 or SEQ ID NO 2, or of Protein Z, as specified by SEQ ID NO 3, wherein at least the glutamine residue at position 9 has been mutated to an amino acid other than asparagine. The invention also discloses multimers of said polypeptide, as well as separation matrices comprising the multimers or polypeptides.

公开(公告)号：US20170120232A1

公开(公告)日：2017-05-04

申请号：US15407240

申请日：2017-01-16

Applicant: GE Healthcare BioProcess R&D AB

Inventor： Jesper Hansson ,  Gustav Rodrigo ,  Tobias E. Soderman

IPC: B01J39/26 ,  B01J20/24 ,  B01D15/36 ,  C07K1/22 ,  B01D15/38 ,  B01D15/34 ,  B01J20/26 ,  B01J20/286

CPC classification number: B01J39/26 ,  B01D15/1864 ,  B01D15/34 ,  B01D15/361 ,  B01D15/362 ,  B01D15/3809 ,  B01J20/24 ,  B01J20/261 ,  B01J20/265 ,  B01J20/267 ,  B01J20/28014 ,  B01J20/28033 ,  B01J20/286 ,  B01J20/289 ,  B01J20/3085 ,  B01J20/3212 ,  B01J20/3278 ,  B01J20/3282 ,  B01J2220/80 ,  C07H1/08 ,  C07K1/22 ,  G01N2030/8827 ,  Y02P20/582

Abstract: The invention discloses a method of separating a biomolecule from at least one other component in a liquid, comprising a step of contacting said liquid with a separation matrix comprising a solid support and polymer chains bound to said solid support. The polymer chains comprise units derived from a first monomer of structure CH2═CH-L-X, where L is a covalent bond or an alkyl ether or hydroxysubstituted alkyl ether chain comprising 2-6 carbon atoms, and X is a sulfonate or phosphonate group.

公开(公告)号：US20170080358A1

公开(公告)日：2017-03-23

申请号：US15367640

申请日：2016-12-02

Applicant: GE Healthcare BioProcess R&D AB

Inventor： Sophia Hober

IPC: B01D15/38 ,  C07K16/06 ,  B01J20/289 ,  C07K14/00 ,  B01J20/24

CPC classification number: B01D15/3809 ,  B01J20/24 ,  B01J20/286 ,  B01J20/289 ,  B01J20/3244 ,  B01J2220/44 ,  B01J2220/52 ,  C07K1/22 ,  C07K14/001 ,  C07K14/195 ,  C07K14/31 ,  C07K16/065 ,  Y10S530/81 ,  Y10S530/825

Abstract: The present invention relates to an immunoglobulin-binding protein, wherein at least one asparagine residue has been mutated to an amino acid other than glutamine or aspartic acid, which mutation confers an increased chemical stability at pH-values of up to about 13-14 compared to the parental molecule. The protein can for example be derived from a protein capable of binding to other regions of the immunoglobulin molecule than the complementarity determining regions (CDR), such as protein A, and preferably the B-domain of Staphylococcal protein A. The invention also relates to a matrix for affinity separation, which comprises an immunoglobulin-binding protein as ligand coupled to a solid support, in which protein ligand at least one asparagine residue has been mutated to an amino acid other than glutamine.

公开(公告)号：US09597610B2

公开(公告)日：2017-03-21

申请号：US13672819

申请日：2012-11-09

Applicant: GE HEALTHCARE BIOPROCESS R&D AB

Inventor： Klaus Gebauer

IPC: B01D15/20 ,  B01D15/22 ,  G01N30/56 ,  B01D15/14 ,  G01N30/60

CPC classification number: B01D15/206 ,  B01J2220/56 ,  G01N30/56 ,  G01N30/6021 ,  G01N30/603 ,  G01N30/606 ,  G01N2030/565 ,  Y10T137/0402

Abstract: The invention relates to a system for packing chromatography columns with a chromatography medium and packing method for use in such columns. In particular, the invention relates to a method and system for packing chromatography columns where the column and chromatography media are pre-sterilized.

公开(公告)号：US09580692B2

公开(公告)日：2017-02-28

申请号：US14380477

申请日：2013-02-27

Applicant: GE Healthcare BioProcess R&D AB

Inventor： Patrik Adielsson ,  Tobias E. Soderman ,  Anna Akerblom

IPC: C12N7/02 ,  B01D15/36 ,  C12N7/00 ,  C07K1/20 ,  B01D15/38 ,  B01J20/24 ,  B01J20/285 ,  B01J20/286

CPC classification number: C12N7/00 ,  B01D15/363 ,  B01D15/3847 ,  B01J20/24 ,  B01J20/285 ,  B01J20/286 ,  C07K1/20 ,  C12N2760/16151

Abstract: The present invention relates to a method for endotoxin removal from a sample comprising the following steps: combining the sample comprising one or more target molecule(s) with a chromatography media comprising beads having an inner porous core functionalized with ligands capable of binding endotoxin and an outer porous layer without functional groups and a pore size small enough to exclude the target molecule from the inner core; and collecting the sample from the media, wherein the sample comprises an endotoxin level which is at least 75% less, preferably 90% less, than before the removal and the yield of the target molecule is at least 75%.

Abstract translation: 本发明涉及一种从样品中去除内毒素的方法，包括以下步骤：将包含一种或多种靶分子的样品与包含珠的色谱介质组合，所述珠粒具有由能够结合内毒素的配体功能化的内部多孔核心和 没有官能团的外部多孔层和足够小以从靶芯排出靶分子的孔径; 并从培养基中收集样品，其中样品包含与去除前相比至少75％以上，优选90％以下的内毒素水平，并且目标分子的产率为至少75％。

公开(公告)号：US09409967B2

公开(公告)日：2016-08-09

申请号：US14348634

申请日：2012-09-28

Applicant: GE HEALTHCARE BIO-SCIENCES AB

Inventor： Eggert Brekkan ,  Kjell Eriksson ,  Bo-Lennart Johansson ,  Jamil Shanagar

IPC: C07K1/18 ,  A61K38/28 ,  B01D15/08 ,  B01D15/26 ,  B01D15/36 ,  B01J20/285 ,  B01J20/286 ,  B01J20/32 ,  C07K14/62 ,  B01J39/26 ,  B01J20/288

CPC classification number: C07K14/62 ,  B01D15/361 ,  B01D15/362 ,  B01J20/286 ,  B01J20/288 ,  B01J20/321 ,  B01J20/3212 ,  B01J20/3293 ,  B01J39/26

Abstract: The present invention is within the field of biomolecule purification. More closely the invention relates to chromatographic purification of insulin using a specific kind of shell beads having an inner core and an outer functionalized layer. The method enables purification at high flow rates and high purity, over 90%.

Abstract translation: 本发明在生物分子纯化领域内。 更具体地，本发明涉及使用具有内核和外功能化层的特定种类的壳珠的色谱纯化胰岛素。 该方法能够在高流速和高纯度下纯化，超过90％。

公开(公告)号：US10933350B2

公开(公告)日：2021-03-02

申请号：US16154639

申请日：2018-10-08

Applicant: GE Healthcare BioProcess R&D AB

Inventor： Klaus Gebauer

IPC: B01D15/14 ,  B01D15/18 ,  B01D15/22 ,  G01N30/60 ,  G01N30/88 ,  G01N30/46

Abstract: A parallel assembly of chromatography column modules, the assembly having one common assembly inlet and one common assembly outlet, each column module comprising a bed space filled with chromatography medium and each column module comprises integrated fluid conduits which when the column module is connected with other column modules are adapted to connect the bed space of the column module with the assembly inlet and the assembly outlet, wherein the total length and/or volume of the fluid conduit from the assembly inlet to one bed space together with the length and/or volume of the fluid conduit from the same bed space to the assembly outlet is substantially the same for all bed spaces and modules installed in the parallel assembly.

公开(公告)号：US20200318120A1

公开(公告)日：2020-10-08

申请号：US16615552

申请日：2018-05-23

Applicant: GE HEALTHCARE BIOPROCESS R&D AB

Inventor： Mats ANDER ,  Magnus BERGMAN ,  Tomas BJORKMAN ,  Joakim GALLI ,  Gustav RODRIGO

IPC: C12N15/62 ,  C07K1/22 ,  C07K14/31

Abstract: A recombinant protein comprising a functional polypeptide and, linked to the N-terminus of said functional polypeptide, an N-terminal spacer having a length such that the number of amino acid residues between a signal peptide cleaving site and an N-terminus proximal structural unit of said functional polypeptide is 14-24.

公开(公告)号：US10751645B2

公开(公告)日：2020-08-25

申请号：US15882998

申请日：2018-01-29

Applicant: GE Healthcare BioProcess R&D AB

Inventor： Carina Engstrand ,  Annika Forss ,  Gunnar Glad ,  Bo-Lennart Johansson ,  Hans J Johansson ,  Jean-Luc Maloisel

IPC: B01D15/38 ,  B01D15/32 ,  B01D15/36 ,  B01J20/32 ,  B01J20/286 ,  B01J41/20 ,  C07K1/36 ,  C07K16/06 ,  C07K1/16 ,  C07K16/00

Abstract: The present invention relates to a chromatography ligand defined by the following formula R1—R2—N(R3)—R4—R5 wherein R1 is a substituted or non-substituted phenyl group; R2 is a hydrocarbon chain comprising 0-4 carbon atoms; R3 is a hydrocarbon chain comprising 1-3 carbon atoms; R4 is a hydrocarbon chain comprising 1-5 carbon atoms; and R5 is OH or H. The invention also comprises a separation matrix, comprising the described ligands coupled to a porous support, such as particles or a membrane. The ligand and matrix according to the invention is useful for purification of biomolecules or organic compounds, such as proteins, polypeptides, DNA etc. An advantageous use according to the invention is the purification of antibodies.

公开(公告)号：US10711035B2

公开(公告)日：2020-07-14

申请号：US16682855

申请日：2019-11-13

Applicant: GE HEALTHCARE BIOPROCESS R&D AB

Inventor： Gustav José Rodrigo ,  Tomas Bjorkman ,  Mats Ander ,  Jesper Ulf Hansson

IPC: A23J1/00 ,  C07K1/22 ,  C07K16/00 ,  C07K14/31 ,  B01J20/28 ,  B01J20/24 ,  B01J20/32 ,  B01D15/38 ,  C07K17/10 ,  B01J20/286 ,  C07K16/06

Abstract: The invention relates to a separation matrix comprising at least 11 mg/ml Fc-binding ligands covalently coupled to a porous support, wherein: a) the ligands comprise multimers of alkali-stabilized Protein A domains, and b) the porous support comprises cross-linked polymer particles having a volume-weighted median diameter (d50,v) of 56-70 micrometers and a dry solids weight of 55-80 mg/ml.