公开(公告)号：US20240240209A1

公开(公告)日：2024-07-18

申请号：US18556152

申请日：2022-04-20

Applicant: Agency for Science, Technology and Research ,  Institut National De Recherche Pour L' Agriculture, L'Alimentation Et L'Environment ,  Centre National De La Recherche Scientifique ,  Institut National Des Sciences Appliquées De Toulouse

Inventor： Xixian CHEN ,  Congqiang ZHANG ,  Rehka T ,  Sudha SHUKAL ,  Derek SMITH ,  Isabelle ANDRÉ ,  Esque JEREMY

IPC: C12P7/26 ,  C12N9/10 ,  C12N9/78 ,  C12N15/52 ,  C12N15/70 ,  C12P41/00

CPC classification number: C12P7/26 ,  C12N9/1007 ,  C12N9/78 ,  C12N15/52 ,  C12N15/70 ,  C12P41/00 ,  C12Y201/01 ,  C12Y201/01067 ,  C12Y305/04028 ,  C12N2800/101

Abstract: Synthesis of enantiopure cis-α-irone from a renewable carbon source Disclosed herein are natural and synthetic enzymes capable of performing a method of producing cis-α-irone. The method comprises providing an enzyme capable of converting psi-ionone to cis-α-irone; and contacting the enzyme and psi-ionone under suitable conditions to produce cis-α-irone. The enzyme may be a methyltransferase from Streptomyces albireticuli (SaMT), a promiscuous bifunctional methyltransferase/cyclase (pMT1) enzyme from Streptomyces, or a modified pMT1 enzyme with at least one substitution. The enzymes allow the in-vivo and in-vitro production of cis-α-irone including the use of glucose as feedstock for the biotransformation into cis-α-irone.

公开(公告)号：US20240043892A1

公开(公告)日：2024-02-08

申请号：US18345379

申请日：2023-06-30

Applicant: Arcturus Therapeutics, Inc.

Inventor： Maher ALAYYOUBI ,  Kyoung-Joo Jenny CHOI ,  Timothy Wayne LUGER, II

IPC: C12P19/34 ,  C12N9/10 ,  C12N9/12

CPC classification number: C12P19/34 ,  C12N9/1007 ,  C12Y201/01 ,  C12N9/1247 ,  C12Y207/07006

Abstract: Disclosed herein are methods of producing transcribed RNA product with increased yield and reduced dsRNA impurities.

公开(公告)号：US20230287362A1

公开(公告)日：2023-09-14

申请号：US18183815

申请日：2023-03-14

Applicant: The Regents of the University of Colorado, a body corporate ,  The Regents of the University of California

Inventor： Aaron Whiteley ,  Hannah Ledvina ,  Kevin Corbett ,  Qiaozhen Ye ,  Yajie Gu

IPC: C12N9/12 ,  C07K16/10 ,  C12N9/10

CPC classification number: C12N9/1241 ,  C07K16/10 ,  C12N9/1007 ,  C12Y201/01 ,  C12N9/1077 ,  C12Y204/02038 ,  C07K2319/30

Abstract: The invention describes the discovery and novel application of a bacterial ubiquitin transferase (Cap2). Specifically, the invention describes the novel activity of the enzyme Cap2 which is capable of creating a specific fusion between two proteins implementing a standalone catalytic mechanism to create the fusion.

公开(公告)号：US20230220443A1

公开(公告)日：2023-07-13

申请号：US16488421

申请日：2018-02-20

Applicant: ALBERT EINSTEIN COLLEGE OF MEDICINE

Inventor： Emmanuel Sebastien BURGOS ,  David SHECHTER

IPC: C12Q1/48 ,  G01N21/76 ,  C12Q1/34

CPC classification number: C12Q1/48 ,  C12Y201/01 ,  C12Y305/04 ,  G01N21/76 ,  C12Q1/34

Abstract: Methods and kits are disclosed for measuring activity of a methyltransferase or a radical SAM enzyme or for screening for an inhibitor of a methyltransferase or a radical SAM enzyme, where the methods and kits comprise, respectively, deaminase TM0936 for a MTase coupled assay and deaminase PA3170 for a radical SAM coupled assay.

公开(公告)号：US20180030484A1

公开(公告)日：2018-02-01

申请号：US15488320

申请日：2017-04-14

Applicant: Genomatica, Inc.

Inventor： Anthony P. Burgard ,  Robin E. Osterhout ,  Stephen J. Van Dien ,  Cara Ann Tracewell ,  Priti Pharkya ,  Stefan Andrae

IPC: C12P7/18 ,  C08G69/26 ,  C08G63/66 ,  C08G63/12 ,  C08G63/06 ,  C12N9/10 ,  C12P19/02 ,  C12P7/24 ,  C08G18/32 ,  C12N9/88 ,  C12N9/04 ,  C12N9/06 ,  C07D207/12

CPC classification number: C12P7/18 ,  C07D207/12 ,  C08G18/3206 ,  C08G63/06 ,  C08G63/12 ,  C08G63/66 ,  C08G69/26 ,  C12N9/0006 ,  C12N9/0028 ,  C12N9/1007 ,  C12N9/88 ,  C12P7/24 ,  C12P19/02 ,  C12Y101/01224 ,  C12Y101/01244 ,  C12Y101/02007 ,  C12Y105/0102 ,  C12Y201/01 ,  C12Y201/0109 ,  C12Y402/01 ,  Y02P20/52

Abstract: Provided herein is a non-naturally occurring microbial organism having a methanol metabolic pathway that can enhance the availability of reducing equivalents in the presence of methanol. Such reducing equivalents can be used to increase the product yield of organic compounds produced by the microbial organism, such as 1,4-butanediol (BDO). Also provided herein are methods for using such an organism to produce BDO.

公开(公告)号：US20170327805A1

公开(公告)日：2017-11-16

申请号：US15415431

申请日：2017-01-25

Applicant: The General Hospital Corporation

Inventor： J. Keith Joung ,  Shengdar Tsai

IPC: C12N9/22 ,  C12N15/10 ,  C12N15/11 ,  C12N2310/20

CPC classification number: C12N15/102 ,  C07K14/005 ,  C07K14/195 ,  C07K2319/00 ,  C07K2319/01 ,  C07K2319/80 ,  C12N9/0071 ,  C12N9/1007 ,  C12N9/16 ,  C12N9/22 ,  C12N9/96 ,  C12N15/01 ,  C12N15/1031 ,  C12N15/11 ,  C12N15/63 ,  C12N15/85 ,  C12N2310/20 ,  C12N2710/00033 ,  C12N2770/00033 ,  C12N2800/80 ,  C12Y114/11 ,  C12Y201/01 ,  C12Y301/00 ,  C12Y301/21004

Abstract: Methods for increasing specificity of RNA-guided genome editing, e.g., editing using CRISPR/Cas9 systems.

公开(公告)号：US20170198318A1

公开(公告)日：2017-07-13

申请号：US15389849

申请日：2016-12-23

Applicant: NATIONAL UNIVERSITY OF TAINAN ,  BIO-JOURDENESS INTERNATIONAL GROUP CO., LTD.

Inventor： TE-SHENG CHANG ,  YA-TING TSAI ,  HSIOU-YU DING ,  CHIEN-MIN CHIANG

IPC: C12P17/06 ,  A61K8/49 ,  A61Q19/02

CPC classification number: C12P17/06 ,  A61K8/498 ,  A61Q19/02 ,  C12Y201/01

Abstract: A method of manufacturing methoxy-isoflavones by biotransformation and a use thereof are revealed herein. The method comprises the steps of synthesizing a nucleic acid sequence including a SpOMT2884 gene ofStreptomyces peucetius; cloning the nucleic acid sequence into an expression vector to form a cyclic recombinant plasmid; transforming the cyclic recombinant plasmid into a microbial expression system (Escherichia coli); and incubating the microbial expression system in a medium containing 8-hydroxydaidzeins therein for generating methoxy-isoflavones.

公开(公告)号：US09657316B2

公开(公告)日：2017-05-23

申请号：US13975678

申请日：2013-08-26

Applicant: Genomatica, Inc.

Inventor： Anthony P. Burgard ,  Robin E. Osterhout ,  Stephen J. Van Dien ,  Cara Ann Tracewell ,  Priti Pharkya ,  Stefan Andrae

IPC: C12P7/24 ,  C12P7/28 ,  C12P7/18 ,  C12N1/20 ,  C12N1/00 ,  C07H21/04 ,  C08G18/00 ,  C08G63/00 ,  C08G63/02 ,  C12N9/04 ,  C12N9/06 ,  C12N9/10 ,  C07D207/12 ,  C08G18/32 ,  C08G63/06 ,  C08G63/12 ,  C08G63/66 ,  C08G69/26 ,  C12N9/88 ,  C12P19/02

CPC classification number: C12P7/18 ,  C07D207/12 ,  C08G18/3206 ,  C08G63/06 ,  C08G63/12 ,  C08G63/66 ,  C08G69/26 ,  C12N9/0006 ,  C12N9/0028 ,  C12N9/1007 ,  C12N9/88 ,  C12P7/24 ,  C12P19/02 ,  C12Y101/01224 ,  C12Y101/01244 ,  C12Y101/02007 ,  C12Y105/0102 ,  C12Y201/01 ,  C12Y201/0109 ,  C12Y402/01 ,  Y02P20/52

Abstract: Provided herein is a non-naturally occurring microbial organism having a methanol metabolic pathway that can enhance the availability of reducing equivalents in the presence of methanol. Such reducing equivalents can be used to increase the product yield of organic compounds produced by the microbial organism, such as 1,4-butanediol (BDO). Also provided herein are methods for using such an organism to produce BDO.

公开(公告)号：US09567604B2

公开(公告)日：2017-02-14

申请号：US14213723

申请日：2014-03-14

Applicant: The General Hospital Corporation

Inventor： J. Keith Joung ,  Jeffry D. Sander ,  Morgan Maeder ,  Yanfang Fu

IPC: C07H21/02 ,  C12N15/00 ,  A61K48/00 ,  C12N15/85 ,  C12N9/22 ,  C12N15/63 ,  C07K14/005 ,  C07K14/195 ,  C12N9/02 ,  C12N9/10 ,  C12N9/16 ,  C12N9/96 ,  C12N15/01

CPC classification number: C12N15/102 ,  C07K14/005 ,  C07K14/195 ,  C07K2319/00 ,  C07K2319/01 ,  C07K2319/80 ,  C12N9/0071 ,  C12N9/1007 ,  C12N9/16 ,  C12N9/22 ,  C12N9/96 ,  C12N15/01 ,  C12N15/1031 ,  C12N15/11 ,  C12N15/63 ,  C12N15/85 ,  C12N2310/20 ,  C12N2710/00033 ,  C12N2770/00033 ,  C12N2800/80 ,  C12Y114/11 ,  C12Y201/01 ,  C12Y301/00 ,  C12Y301/21004

Abstract: Methods for increasing specificity of RNA-guided genome editing, e.g., editing using CRISPR/Cas9 systems, using truncated guide RNAs (tru-gRNAs).

Abstract translation: 使用截短的引导RNA（tru-gRNA）增加RNA指导基因组编辑的特异性的方法，例如使用CRISPR / Cas9系统的编辑。

公开(公告)号：US20160340686A1

公开(公告)日：2016-11-24

申请号：US14901904

申请日：2014-06-27

Applicant: Federica Brandizzi ,  Curtis Wilkerson ,  Sang Jin Kim ,  Michael Held

Inventor： Federica Brandizzi ,  Curtis Wilkerson ,  Sang Jin Kim ,  Michael Held

IPC: C12N15/82 ,  C12P19/02 ,  C12N9/10 ,  C12P19/14

CPC classification number: C12N15/8261 ,  C12N9/1007 ,  C12P19/02 ,  C12P19/14 ,  C12Y201/01 ,  Y02A40/146

Abstract: Plants described herein have increased biomass and are more readily digested into fermentable sugars when the plants express increased levels of one or more types of CGR2 and/or CGR3 enzymes.

Abstract translation: 当植物表达增加水平的一种或多种类型的CGR2和/或CGR3酶时，本文所述的植物具有增加的生物量并且更容易地被消化成可发酵的糖。