公开(公告)号：US20140173876A1

公开(公告)日：2014-06-26

申请号：US14191499

申请日：2014-02-27

Applicant: EMD Millipore Corporation

Inventor： James Hobson ,  James Austin ,  Boris Pesakovich ,  Elias G. Noukas ,  Thomas Janko ,  Shaun McMahon

IPC: B65F1/06

CPC classification number: A61J1/10 ,  B65B69/0091 ,  B65D88/20 ,  B65D90/046 ,  B65D2203/10 ,  B65D2303/00 ,  B65F1/06 ,  Y10T29/49826

Abstract: The present invention is an alignment device for a bag containing one or more ports. The alignment device is attached to either the one or more ports or to the bag adjacent the one or more ports. The plate has an indicator or a unique outer edge shape that is designed to fit into a corresponding unique opening in a holder to ensure proper alignment of the bag and its port(s) in the holder. The plate is has a Series of holes equal to and in alignment with the one or more ports of the bag and the port(s) extend through the holes of the plate,

Abstract translation: 本发明是一种用于包含一个或多个端口的袋的对准装置。 对准装置附接到一个或多个端口或邻近一个或多个端口的袋。 该板具有指示器或独特的外边缘形状，其被设计成装配在保持器中相应的独特开口中，以确保袋和其端口在保持器中的适当对准。 板具有与袋的一个或多个端口相等并且对准的一系列孔，并且该端口延伸穿过板的孔，

公开(公告)号：US20140090735A1

公开(公告)日：2014-04-03

申请号：US14053688

申请日：2013-10-15

Applicant: EMD Millipore Corporation

Inventor： Paul Ju-Sung Hung ,  Philip Janmin Lee

IPC: F16K27/02

CPC classification number: C12M23/12 ,  B01L3/5025 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502738 ,  B01L3/502746 ,  B01L2200/12 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/10 ,  B01L2300/123 ,  B01L2300/14 ,  B01L2400/0481 ,  B01L2400/0487 ,  B01L2400/0655 ,  B29C65/002 ,  C12M23/16 ,  C12M29/10 ,  C12M29/14 ,  C12M41/00 ,  C12M41/48 ,  C12Q1/025 ,  F16K27/0236 ,  F16K99/0001 ,  F16K99/0015 ,  F16K99/0059 ,  F16K2099/0074 ,  F16K2099/0078 ,  F16K2099/0084 ,  G01N33/5008 ,  G01N33/54366 ,  G01N2035/00158 ,  Y10T137/2202

Abstract: A valved microfluidics device, microfluidics cell-culture device and system incorporating the devices are disclosed. The valved microfluidics device includes a substrate, a microchannel through which liquid can be moved from one station to another within the device, and a pneumatic microvalve adapted to be switched between open and closed states to control the flow of fluid through a microchannel. The microvalve is formed of three flexible membranes, one of which is responsive to pneumatic pressure applied to the valve and the other two of which deform to produce a more sealable channel cross-section. The cell culture device provides valving to allow controlled loading of cells into the individual well of the device, and exchange of cell-culture components in the wells.

Abstract translation: 公开了一种带阀的微流体装置，微流体细胞培养装置和结合装置的系统。 带阀的微流体装置包括基底，微通道，通过该微通道液体可以在装置内从一个站移动到另一个站;以及气动微型阀，其适于在打开和关闭状态之间切换以控制通过微通道的流体流动。 微型阀由三个柔性膜形成，其中一个柔性膜响应于施加到阀上的气压，另外两个变形以产生更可密封的通道横截面。 细胞培养装置提供阀门以允许将细胞控制加载到装置的个体井中，以及在孔中交换细胞培养组分。

公开(公告)号：US08672144B2

公开(公告)日：2014-03-18

申请号：US13669494

申请日：2012-11-06

Applicant: EMD Millipore Corporation

Inventor： David P. Yavorsky

IPC: B01D39/20 ,  B01J20/14

CPC classification number: B01D39/2079 ,  B01D2239/0407 ,  B01D2239/08 ,  B01J20/10 ,  B01J20/103 ,  B01J20/14 ,  B01J20/165 ,  B01J20/18 ,  B01J20/20 ,  B01J20/28026 ,  B01J20/2803

Abstract: Adsorbent filter media particularly suited for removal of biological contaminants in process liquids. A porous fixed bed of adsorbent material is formed, using only a granular adsorbent and a water-insoluble thermoplastic binder. The resulting composite filter allows for a higher amount of adsorbent with smaller adsorbent particles than conventional depth filters. Elimination of cellulose fiber, as well as the elimination of the thermoset binder, results in reduced contamination of the process liquid.

Abstract translation: 吸附剂过滤介质特别适用于去除工艺液体中的生物污染物。 仅使用粒状吸附剂和水不溶性热塑性粘合剂形成吸附剂材料的多孔固定床。 所得到的复合过滤器允许比常规深度过滤器更大量的具有较小吸附剂颗粒的吸附剂。 消除纤维素纤维以及消除热固性粘合剂导致工艺液体的污染减少。

公开(公告)号：US20140046037A1

公开(公告)日：2014-02-13

申请号：US14061080

申请日：2013-10-23

Applicant: EMD Millipore Corporation

Inventor： Shari Spector

IPC: C07K16/12

CPC classification number: C07K16/1271 ,  B01J20/286 ,  B01J20/3274 ,  C07K1/22 ,  C07K14/31

Abstract: The present invention relates to modified immunoglobulin-binding proteins, e.g., Staphylococcus protein A, having improved binding specificity for immunoglobulins and methods of making and using the same.

Abstract translation: 本发明涉及对免疫球蛋白具有改善的结合特异性的修饰的免疫球蛋白结合蛋白，例如葡萄球菌蛋白A，以及制备和使用免疫球蛋白的方法。

公开(公告)号：US20140038226A1

公开(公告)日：2014-02-06

申请号：US14047373

申请日：2013-10-07

Applicant: EMD Millipore Corporation

Inventor： Christian Schann ,  Bruno Duquet ,  Abdel Hajila ,  Frederic Olivieri

IPC: C12Q1/24

CPC classification number: C12Q1/24 ,  B05B12/20 ,  G01N21/763

Abstract: The device for spraying a reagent onto a support (81) adapted to retain microorganisms on a predetermined surface (82), comprises a spraying bell (3) as well as a nozzle (71) for emitting a jet of droplets of said reagent into a spraying chamber (34) comprised by said bell (3), said device also comprising an absorbent pad (5) mounted against said bell (3) transversely to said jet and closing said chamber (34) from the opposite side to said nozzle (71) with the exception of a circular central opening (51) provided in said pad (5), the diameter of said central opening (51) being adapted to enable a portion of said jet, when said device faces said support (81) and is at a predetermined distance therefrom, to pass through said central opening (51) over its entire area and be deposited on the whole of said predetermined surface (82) of said support (81).

Abstract translation: 用于将试剂喷洒到适于将微生物保留在预定表面（82）上的支撑体（81）上的装置包括喷雾钟（3）以及喷嘴（71），用于将所述试剂的液滴喷射到 喷雾室（34），所述装置还包括一个吸收垫（5），该吸收垫（5）横向于所述射流安装在所述钟形（3）上，并从所述喷嘴（71）的相对侧关闭所述腔室 ），除了设置在所述垫（5）中的圆形中心开口（51）之外，所述中心开口（51）的直径适于使所述装置的一部分当所述装置面向所述支撑件（81）并且是 在其预定距离处穿过所述中心开口（51）遍及其整个区域并且沉积在所述支撑件（81）的整个所述预定表面（82）上。

公开(公告)号：US20130317204A1

公开(公告)日：2013-11-28

申请号：US13955024

申请日：2013-07-31

Applicant: EMD Millipore Corporation

Inventor： Wilson Moya ,  Jad Jaber

IPC: C07K1/32

CPC classification number: C07K1/32 ,  C07K1/30

Abstract: The present invention relates to a selectively soluble polymer capable of binding to a desired molecules in an unclarified mixture containing various biological materials and the methods of using such a polymer to purify a molecule from such a mixture. The polymer is soluble in the mixture under a certain set of process conditions such as pH or temperature and/or salt concentration and is rendered insoluble and precipitates out of solution upon a change in the process conditions. The polymer is capable of binding to the desired molecule (protein, polypeptide, etc) and remains capable of binding to that molecule even after the polymer is precipitated out of solution. The precipitate can then be filtered out from the remainder of the stream and the desired biomolecule is recovered such as by elution and further processed.

Abstract translation: 本发明涉及能够在含有各种生物材料的未澄清混合物中结合所需分子的选择性可溶性聚合物以及使用这种聚合物从这种混合物中纯化分子的方法。 聚合物在一定的工艺条件例如pH或温度和/或盐浓度下可溶于混合物中，并且在工艺条件改变时变得不溶解并沉淀出溶液。 聚合物能够结合到所需的分子（蛋白质，多肽等），并且即使在聚合物从溶液中沉淀出来之后仍然能够结合该分子。 然后可以从流的其余部分过滤出沉淀物，并回收所需的生物分子，例如通过洗脱和进一步处理。

公开(公告)号：US20130240065A1

公开(公告)日：2013-09-19

申请号：US13889616

申请日：2013-05-08

Applicant: EMD MILLIPORE CORPORATION

Inventor： Jean-Louis Weissenbach ,  Sebastien Cirou

IPC: B01D63/00

CPC classification number: B01D63/00 ,  B01D61/20 ,  B01D2313/105 ,  B01D2313/125 ,  B01D2313/13 ,  B01D2313/18 ,  B01D2313/58 ,  B01D2313/90 ,  B01D2315/10 ,  Y10T137/6851 ,  Y10T137/8593

Abstract: The invention concerns a bag comprising a first conduit (13C) which extends longitudinally between a flow pump connector (11C) emerging on a first side (68) and a tangential filter connector (11M) emerging on a second side (69); a second conduit (13B) which extends longitudinally from a first side of said conduit (13C) between a supply container connector (11B) emerging on said first side (68), and another tangential flow connector (11N) emerging on said second side (69); a third conduit (13H) which extends from a second side of said conduit (13C), starting at a collecting container connector (11J), until it enters said first conduit (13C); and a fourth conduit (13A) which extends from the first side of said conduit (13C), starting at a transfer pump connector (11A), until it enters said second conduit (13B).

Abstract translation: 本发明涉及一种袋子，其包括在出现在第一侧面（68）上的流动泵连接器（11C）和出现在第二侧面（69）上的切向过滤器连接器（11M）之间纵向延伸的第一管道（13C）。 第二导管（13B），其从所述导管（13C）的第一侧沿着出现在所述第一侧（68）上的供应容器连接器（11B）和出现在所述第二侧（68）上的另一个切向流动连接器（11N） 69）; 从所述导管（13C）的第二侧延伸的第三导管（13H），从收集容器连接器（11J）开始，直至其进入所述第一导管（13C）; 以及从所述导管（13C）的第一侧从转移泵连接器（11A）开始直到其进入所述第二导管（13B）的第四导管（13A）。

公开(公告)号：US20130236130A1

公开(公告)日：2013-09-12

申请号：US13872248

申请日：2013-04-29

Applicant: EMD MILLIPORE CORPORATION

Inventor： Sebastien Cirou ,  Rene Reinbigler ,  Virginie Buisson ,  Jean-Louis Weissenbach

IPC: B65D33/00

CPC classification number: B65D33/00 ,  A61M1/36 ,  A61M2205/125 ,  A61M2209/088 ,  B01D61/18 ,  B01D61/20 ,  B01D2313/105 ,  B01D2313/125 ,  B01D2313/58 ,  F04B43/08

Abstract: The invention concerns a device comprising a base (2) and a door (20), said device having a closed door position in which a circuit (8) of the device comprises a bag comprising two flexible films and connectors of the conveying network, and a press (9) comprising a first shell (16) disposed on a front face (5) of said base (2) and a second shell (17) disposed in said door (20); and a hinge system hinging said door (20) relative to said base (2), and disposed only on one side of said door (20) so as to form lateral clearances between said door (20) and said base (2) over the rest of a perimeter of said door (20)

Abstract translation: 本发明涉及一种包括基座（2）和门（20）的装置，所述装置具有关闭的门位置，其中装置的电路（8）包括包括输送网络的两个柔性膜和连接器的袋，以及 包括设置在所述基座（2）的前表面（5）上的第一壳体（16）和设置在所述门（20）中的第二壳体（17）的压力机（9）。 以及铰链系统，其将所述门（20）相对于所述基座（2）铰接，并且仅设置在所述门（20）的一侧上，以便在所述门（20）和所述基座（2）之间形成横向间隙 所述门（20）的周边的其余部分

公开(公告)号：US20130171682A1

公开(公告)日：2013-07-04

申请号：US13761130

申请日：2013-02-06

Applicant: EMD Millipore Corporation

Inventor： Ju-Sung Paul Hung ,  Philip J. Lee ,  Amedeo J. Cappione, III

IPC: C12Q1/02 ,  C12M3/06

CPC classification number: C12M23/16 ,  B01L3/502715 ,  B01L3/502753 ,  B01L2200/027 ,  B01L2200/0647 ,  B01L2200/0694 ,  B01L2300/0681 ,  B01L2300/0877 ,  B01L2300/14 ,  C12M23/34 ,  C12M23/40 ,  C12M27/18 ,  C12M29/10 ,  C12M41/46 ,  C12Q1/02 ,  C12Q1/025

Abstract: A number of novel improved microfluidic configurations and systems and methods of manufacture and operation for a microfluidic invasion assay system.

Abstract translation: 许多新颖的改进的微流体配置和用于微流体入侵测定系统的制造和操作的系统和方法。

公开(公告)号：US20130130356A1

公开(公告)日：2013-05-23

申请号：US13732870

申请日：2013-01-02

Applicant: EMD MILLIPORE CORPORATION

Inventor： Shawn Bates ,  Kerry Roche Lentine ,  Nada Bsat

IPC: C12N1/20

CPC classification number: C12N1/20 ,  C12Q1/02 ,  C12Q1/22

Abstract: A method for retention testing sterilizing grade filters comprises: a) providing a stock of Acholeplasma laidlawii; b) growing up the stock of A. laidlawii for about 24 hours or less in a single serum-free growth medium that supports cell growth to a high titer and yields a cellular morphology where the cells are small, deaggregated and spherical, thereby producing a bacterial culture; c) challenging a test filter by filtering the bacterial culture through the test filter at a known challenge level, thereby producing a filtrate downstream of the test filter; and d) detecting concentration of A. laidlawii in the filtrate. Serum-free growth media for cultivating or storing A. laidlawii are also described.

Abstract translation: 一种杀菌级过滤器的保留测试方法包括：a）提供普通Ac a草; b）在支持细胞生长至高滴度的单一无血清生长培养基中培养约24小时或更少的A.laylawii的存量，并产生细胞形态，其中细胞小，解聚和球形，从而产生 细菌培养; c）通过在已知的攻击级别通过测试过滤器过滤细菌培养物来挑战测试过滤器，从而在测试过滤器的下游产生滤液; 和d）检测滤液中的嗜。菌浓度。 还描述了用于培养或储存A.lllawii的无血清生长培养基。