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公开(公告)号：US20180154281A1
公开(公告)日：2018-06-07
申请号：US15882998
申请日：2018-01-29
Applicant: GE Healthcare BioProcess R&D AB
Inventor： CARINA ENGSTRAND , ANNIKA FORSS , GUNNAR GLAD , BO-LENNART JOHANSSON , HANS J JOHANSSON , JEAN-LUC MALOISEL
IPC: B01D15/38 , B01J20/286 , C07K16/00 , B01D15/32 , B01D15/36 , C07K16/06 , B01J20/32 , B01J41/20 , C07K1/16 , C07K1/36
Abstract: The present invention relates to a chromatography ligand defined by the following formula R1—R2—N(R3)—R4—R5 wherein R1 is a substituted or non-substituted phenyl group; R2 is a hydrocarbon chain comprising 0-4 carbon atoms; R3 is a hydrocarbon chain comprising 1-3 carbon atoms; R4 is a hydrocarbon chain comprising 1-5 carbon atoms; and R5 is OH or H. The invention also comprises a separation matrix, comprising the described ligands coupled to a porous support, such as particles or a membrane. The ligand and matrix according to the invention is useful for purification of biomolecules or organic compounds, such as proteins, polypeptides, DNA etc. An advantageous use according to the invention is the purification of antibodies.
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公开(公告)号：US09896486B2
公开(公告)日：2018-02-20
申请号：US14903837
申请日：2014-07-08
Applicant: GE Healthcare BioProcess R&D AB
Inventor： Gustav Rodrigo , Mats Ander , Tomas Bjorkman , Goran Bauren
IPC: C07K14/31 , C07K1/22 , B01D15/38 , B01J20/286 , B01J20/32 , C07K14/745 , C07K16/00
CPC classification number: C07K14/31 , B01D15/3809 , B01J20/286 , B01J20/3204 , B01J20/3212 , B01J20/3219 , B01J20/3274 , C07K1/22 , C07K14/745 , C07K16/00
Abstract: A polypeptide with improved alkaline stability, which polypeptide comprises a mutant of a B or C domain of Staphylococcus Protein A, as specified by SEQ ID NO 1 or SEQ ID NO 2, or of Protein Z, as specified by SEQ ID NO 3, wherein at least the glutamine residue at position 9 has been mutated to an amino acid other than asparagine. The invention also discloses multimers of said polypeptide, as well as separation matrices comprising the multimers or polypeptides.
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公开(公告)号：US20180036445A1
公开(公告)日：2018-02-08
申请号：US15554075
申请日：2016-02-25
Applicant: GE HEALTHCARE BIOPROCESS R&D AB
Inventor： Elin Marianne Monie , Tomas Bjorkman , Anna Gronberg , Anders Ljunglof , Gustav Jose Rodrigo , Karin Torstenson , Magnus Carl Erik Wetterhall
CPC classification number: A61L2/18 , B01D15/203 , B01D15/3804 , G01N30/50
Abstract: The invention discloses a method for cleaning or sanitization of an affinity chromatography matrix, comprising the steps of: a) providing an affinity chromatography matrix having oxidation-tolerant proteinaceous ligands coupled to a support, b) contacting the matrix with a sanitization solution comprising at least one oxidant defined by formula I, R—O—O—H (I) wherein R is hydrogen or an acyl group R′—C(O)—, with R′ being a hydrogen or a methyl, ethyl or propyl group.
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公开(公告)号：US20170327545A1
公开(公告)日：2017-11-16
申请号：US15526849
申请日：2015-12-11
Applicant: GE HEALTHCARE BIOPROCESS R&D AB
Inventor： Gustav Rodrigo , Mats Ander , Tomas Bjorkman
IPC: C07K14/195 , C07K1/22 , C07K16/00
CPC classification number: C07K14/195 , C07K1/22 , C07K16/00 , C07K2317/55 , C07K2317/569 , C07K2319/02
Abstract: A kappa light chain-binding polypeptide comprising or consisting essentially of one or more binding domains of Peptostreptococcus Protein L, each of said domains being selected from the group consisting of domain 2, domain 3 and domain 4.
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公开(公告)号：US20170327534A1
公开(公告)日：2017-11-16
申请号：US15282367
申请日：2016-09-30
Applicant: GE Healthcare BioProcess R&D AB
Inventor： Gustav Jose RODRIGO , Tomas BJORKMAN , Mats Ander , Jesper Ulf Hansson
IPC: C07K1/22 , B01J20/285 , B01J20/26 , B01J20/286 , C07K17/10 , C07K16/12
CPC classification number: C07K1/22 , B01J20/267 , B01J20/285 , B01J20/286 , B01J20/3274 , B01J2220/52 , C07K14/31 , C07K16/00 , C07K16/1271 , C07K17/10
Abstract: The invention relates to a separation matrix comprising at least 11 mg/ml Fc-binding ligands covalently coupled to a porous support, wherein: a) the ligands comprise multimers of alkali-stabilized Protein A domains, and b) the porous support comprises cross-linked polymer particles having a volume-weighted median diameter (d50,v) of 56-70 micrometers and a dry solids weight of 55-80 mg/ml.
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Patent Agency Ranking
公开(公告)号：US20170120232A1
公开(公告)日：2017-05-04
申请号：US15407240
申请日：2017-01-16
Applicant: GE Healthcare BioProcess R&D AB
Inventor： Jesper Hansson , Gustav Rodrigo , Tobias E. Soderman
CPC classification number: B01J39/26 , B01D15/1864 , B01D15/34 , B01D15/361 , B01D15/362 , B01D15/3809 , B01J20/24 , B01J20/261 , B01J20/265 , B01J20/267 , B01J20/28014 , B01J20/28033 , B01J20/286 , B01J20/289 , B01J20/3085 , B01J20/3212 , B01J20/3278 , B01J20/3282 , B01J2220/80 , C07H1/08 , C07K1/22 , G01N2030/8827 , Y02P20/582
Abstract: The invention discloses a method of separating a biomolecule from at least one other component in a liquid, comprising a step of contacting said liquid with a separation matrix comprising a solid support and polymer chains bound to said solid support. The polymer chains comprise units derived from a first monomer of structure CH2═CH-L-X, where L is a covalent bond or an alkyl ether or hydroxysubstituted alkyl ether chain comprising 2-6 carbon atoms, and X is a sulfonate or phosphonate group.
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公开(公告)号：US20170080358A1
公开(公告)日：2017-03-23
申请号：US15367640
申请日：2016-12-02
Applicant: GE Healthcare BioProcess R&D AB
Inventor： Sophia Hober
IPC: B01D15/38 , C07K16/06 , B01J20/289 , C07K14/00 , B01J20/24
CPC classification number: B01D15/3809 , B01J20/24 , B01J20/286 , B01J20/289 , B01J20/3244 , B01J2220/44 , B01J2220/52 , C07K1/22 , C07K14/001 , C07K14/195 , C07K14/31 , C07K16/065 , Y10S530/81 , Y10S530/825
Abstract: The present invention relates to an immunoglobulin-binding protein, wherein at least one asparagine residue has been mutated to an amino acid other than glutamine or aspartic acid, which mutation confers an increased chemical stability at pH-values of up to about 13-14 compared to the parental molecule. The protein can for example be derived from a protein capable of binding to other regions of the immunoglobulin molecule than the complementarity determining regions (CDR), such as protein A, and preferably the B-domain of Staphylococcal protein A. The invention also relates to a matrix for affinity separation, which comprises an immunoglobulin-binding protein as ligand coupled to a solid support, in which protein ligand at least one asparagine residue has been mutated to an amino acid other than glutamine.
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公开(公告)号：US09597610B2
公开(公告)日：2017-03-21
申请号：US13672819
申请日：2012-11-09
Applicant: GE HEALTHCARE BIOPROCESS R&D AB
Inventor： Klaus Gebauer
CPC classification number: B01D15/206 , B01J2220/56 , G01N30/56 , G01N30/6021 , G01N30/603 , G01N30/606 , G01N2030/565 , Y10T137/0402
Abstract: The invention relates to a system for packing chromatography columns with a chromatography medium and packing method for use in such columns. In particular, the invention relates to a method and system for packing chromatography columns where the column and chromatography media are pre-sterilized.
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公开(公告)号：US09580692B2
公开(公告)日：2017-02-28
申请号：US14380477
申请日：2013-02-27
Applicant: GE Healthcare BioProcess R&D AB
Inventor： Patrik Adielsson , Tobias E. Soderman , Anna Akerblom
IPC: C12N7/02 , B01D15/36 , C12N7/00 , C07K1/20 , B01D15/38 , B01J20/24 , B01J20/285 , B01J20/286
CPC classification number: C12N7/00 , B01D15/363 , B01D15/3847 , B01J20/24 , B01J20/285 , B01J20/286 , C07K1/20 , C12N2760/16151
Abstract: The present invention relates to a method for endotoxin removal from a sample comprising the following steps: combining the sample comprising one or more target molecule(s) with a chromatography media comprising beads having an inner porous core functionalized with ligands capable of binding endotoxin and an outer porous layer without functional groups and a pore size small enough to exclude the target molecule from the inner core; and collecting the sample from the media, wherein the sample comprises an endotoxin level which is at least 75% less, preferably 90% less, than before the removal and the yield of the target molecule is at least 75%.
Abstract translation: 本发明涉及一种从样品中去除内毒素的方法，包括以下步骤：将包含一种或多种靶分子的样品与包含珠的色谱介质组合，所述珠粒具有由能够结合内毒素的配体功能化的内部多孔核心和 没有官能团的外部多孔层和足够小以从靶芯排出靶分子的孔径; 并从培养基中收集样品，其中样品包含与去除前相比至少75％以上，优选90％以下的内毒素水平，并且目标分子的产率为至少75％。
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公开(公告)号：US20200239517A1
公开(公告)日：2020-07-30
申请号：US16095721
申请日：2017-05-10
Applicant: GE HEALTHCARE BIOPROCESS R&D AB
Inventor： Annika Forss , Gustav José Rodrigo , Tomas Bjorkman , Mats Ander , Jesper Ulf Hansson
Abstract: The present invention concerns a method of storing a separation matrix comprising multimers of immunoglobulin-binding alkali-stabilized Protein A domains covalently coupled to a porous support. The method comprises the steps of: a) providing a storage liquid comprising at least 50% by volume of an aqueous alkali metal hydroxide solution; b) permeating the separation matrix with the storage liquid; and c) storing the storage liquid-permeated separation matrix for a storage time of at least days. The alkali-stabilized Protein A domains comprise mutants of a parental Fc-binding domain of Staphylococcus Protein A (SpA), as defined by, or having at least 80% such as at least 90%, 95% or 98% identity to, SEQ ID NO 51 or SEQ ID NO 52, wherein the amino acid residues at positions 13 and 44 of SEQ ID NO 51 or 52 are asparagines and wherein at least the asparagine residue at position 3 of SEQ ID NO 51 or 52 has been mutated to an amino acid selected from the group consisting of glutamic acid, lysine, tyrosine, threonine, phenylalanine, leucine, isoleucine, tryptophan, methionine, valine, alanine, histidine and arginine.
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