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52 条记录 (耗时 0.031 秒)

    Use of aniline derivates as coupling components in oxidative color formation reactions
    32.
    发明授权
    Use of aniline derivates as coupling components in oxidative color formation reactions 失效
    在氧化色彩形成反应中苯胺衍生物作为偶联组分的使用

    公开(公告)号：US4845030A

    公开(公告)日：1989-07-04

    申请号：US122600

    申请日：1987-11-20

    申请人： Hans-Georg Batz ,  Rupert Herrmann ,  Fritz Topfmeier ,  Helmut Schlumberger

    发明人： Hans-Georg Batz ,  Rupert Herrmann ,  Fritz Topfmeier ,  Helmut Schlumberger

    IPC分类号： G01N33/52 ,  C09B57/00 ,  C12Q1/28 ,  G01N21/77 ,  G01N21/78 ,  G01N33/50

    CPC分类号： C12Q1/28 ,  C12Q2326/00 ,  Y10T436/144444 ,  Y10T436/206664

    摘要： The present invention is concerned with the use of aniline derivatives of the general formula: ##STR1## wherein R.sup.1 is a hydrogen atom or a --(CH.sub.2).sub.n --X radical, in which n is a whole number of from 1 to 3, X is H, OH, NH.sub.2, CH.sub.3 CONH, CH.sub.3 SO.sub.2 NH, COOH, SO.sub.3 H or --ArSO.sub.3 H, Ar being an optionally substituted arylene radical, m is a whole number of from 2 to 4, R.sup.3 is a hydrogen atom, an alkyl radical containing up to 3 carbon atoms, OCH.sub.3, CH.sub.3 CONH, COOH or SO.sub.3 H and R.sup.4 is a hydrogen, chlorine or bromine atom or a carboxylic acid or sulphonic acid group, as coupling components in oxidative color formation reactions.The present invention also provides an agent for the analytical determination of oxidizing substances by oxidative color coupling which, as coupling component, contains at least one compound of general formula (I).

    摘要翻译： 本发明涉及以下通式的苯胺衍生物的用途：其中R 1为氢原子或 - （CH 2）n X基团，其中n为1至3的整数， X是H，OH，NH 2，CH 3 CONH，CH 3 SO 2 NH，COOH，SO 3 H或-ArSO 3 H，Ar是任选取代的亚芳基，m是整数2至4，R 3是氢原子， 3个碳原子，OCH 3，CH 3 CONH，COOH或SO 3 H，R 4是氢，氯或溴原子或羧酸或磺酸基团，作为氧化颜色形成反应中的偶联组分。 本发明还提供了一种通过氧化颜色偶联分析测定氧化物质的试剂，其作为偶联组分含有至少一种通式（I）的化合物。

    Preparation of samples for LC-MS/MS using magnetic particles
    33.
    发明授权
    Preparation of samples for LC-MS/MS using magnetic particles 有权
    使用磁性颗粒制备LC-MS / MS样品

    公开(公告)号：US07815803B2

    公开(公告)日：2010-10-19

    申请号：US12133545

    申请日：2008-06-05

    申请人： Uwe Kobold ,  Albert Geiger ,  Rupert Herrmann ,  Michael Vogeser

    发明人： Uwe Kobold ,  Albert Geiger ,  Rupert Herrmann ,  Michael Vogeser

    IPC分类号： B01D15/08 ,  B01D59/44 ,  H01J49/00

    CPC分类号： G01N33/6848 ,  B01D15/325 ,  B01J20/28009 ,  B01J20/287 ,  B01J20/3246 ,  B01J20/3276 ,  G01N33/54326 ,  G01N33/6842

    摘要： The present invention provides a novel procedure of preparing samples for analysis by way of mass spectrometry, preferably LC-MS/MS. Accordingly, functionalized magnetic particles with a hydrophobic surface were used for extracting low molecular weight compounds from complex liquid biological samples such as plasma, serum, whole blood or hemolyzed blood. The method of the invention includes (a) contacting the sample with an amount of functionalized magnetic particles with a hydrophobic surface, (b) incubating the sample and the particles, thereby adsorbing the compound to the hydrophobic surface, (c) separating the particles by applying a magnetic field and removing the liquid, (d) optionally washing the particles, (e) eluting the compound from the particles.

    摘要翻译： 本发明提供了通过质谱法，优选LC-MS / MS制备用于分析的样品的新方法。 因此，使用具有疏水性表面的官能化磁性颗粒从复杂的液体生物样品如血浆，血清，全血或溶血中提取低分子量化合物。 本发明的方法包括（a）使样品与一定量的具有疏水性表面的官能化磁性颗粒接触，（b）将样品和颗粒温育，从而将化合物吸附到疏水表面，（c）将颗粒分离 施加磁场并除去液体，（d）任选地洗涤颗粒，（e）从颗粒中洗脱化合物。

    DIFFERENTIAL HEMOLYSIS OF A WHOLE BLOOD SAMPLE
    34.
    发明申请
    DIFFERENTIAL HEMOLYSIS OF A WHOLE BLOOD SAMPLE 有权
    全血样品的差异分析

    公开(公告)号：US20090253210A1

    公开(公告)日：2009-10-08

    申请号：US12328890

    申请日：2008-12-05

    申请人： Uwe Kobold ,  Thomas Duelffer ,  Rupert Herrmann ,  Herbert von der Eltz

    发明人： Uwe Kobold ,  Thomas Duelffer ,  Rupert Herrmann ,  Herbert von der Eltz

    IPC分类号： G01N33/48 ,  H01J49/00

    CPC分类号： G01N30/06 ,  G01N30/603 ,  G01N30/7233 ,  G01N33/5002 ,  G01N2030/027 ,  Y10T436/24 ,  Y10T436/25

    摘要： The invention relates to a method for differentially hemolyzing whole blood. It discloses method for detecting an analyte in a liquid sample known or suspected to comprise red blood cells and suspected or known to comprise eukaryotic cells, the method comprising the steps of processing said liquid sample with a membrane solubilizing agent under conditions appropriate to lyse cell membranes of red blood cells and at the same time not to cause precipitation of sample constituents, subjecting the processed sample to a chromatographic separation, and detecting the analyte. The differential hemolysis of red blood cells is of advantage in a method of detecting an analyte in a liquid sample that may comprise both erythrocytes as well as nucleated cells. The differential solubilization of red blood cells can be easily combined with an online detection methodology, like LC-MS, and is advantageous in the detection of many analytes, e.g. in the detection of folate or of immunosuppressive drugs, like tacrolimus or sirolimus.

    摘要翻译： 本发明涉及一种使全血不同溶血的方法。 它公开了用于检测已知或怀疑包含红细胞并且怀疑或已知包含真核细胞的液体样品中的分析物的方法，所述方法包括以下步骤：在适合于裂解细胞膜的条件下用膜增溶剂处理所述液体样品 的红细胞，同时不会引起样品成分沉淀，对经处理的样品进行色谱分离，并检测分析物。 在检测可能包含红细胞和有核细胞的液体样品中的分析物的方法中，红细胞的差异溶血是有利的。 红细胞的差异溶解可以容易地与诸如LC-MS的在线检测方法组合，并且在许多分析物的检测中是有利的，例如， 在检测叶酸或免疫抑制药物，如他克莫司或西罗莫司。

    PREPARATION OF SAMPLES FOR LC-MS/MS USING MAGNETIC PARTICLES
    35.
    发明申请
    PREPARATION OF SAMPLES FOR LC-MS/MS USING MAGNETIC PARTICLES 有权
    使用磁性颗粒制备LC-MS / MS样品

    公开(公告)号：US20090090855A1

    公开(公告)日：2009-04-09

    申请号：US12133545

    申请日：2008-06-05

    申请人： Uwe Kobold ,  Albert Geiger ,  Rupert Herrmann ,  Michael Vogeser

    发明人： Uwe Kobold ,  Albert Geiger ,  Rupert Herrmann ,  Michael Vogeser

    IPC分类号： H01J49/00 ,  B01D15/26

    CPC分类号： G01N33/6848 ,  B01D15/325 ,  B01J20/28009 ,  B01J20/287 ,  B01J20/3246 ,  B01J20/3276 ,  G01N33/54326 ,  G01N33/6842

    摘要： The present invention provides a novel procedure of preparing samples for analysis by way of mass spectrometry, preferably LC-MS/MS. Accordingly, functionalized magnetic particles with a hydrophobic surface were used for extracting low molecular weight compounds from complex liquid biological samples such as plasma, serum, whole blood or hemolyzed blood. The method of the invention includes (a) contacting the sample with an amount of functionalized magnetic particles with a hydrophobic surface, (b) incubating the sample and the particles, thereby adsorbing the compound to the hydrophobic surface, (c) separating the particles by applying a magnetic field and removing the liquid, (d) optionally washing the particles, (e) eluting the compound from the particles.

    摘要翻译： 本发明提供了通过质谱法，优选LC-MS / MS制备用于分析的样品的新方法。 因此，使用具有疏水性表面的官能化磁性颗粒从复杂的液体生物样品如血浆，血清，全血或溶血中提取低分子量化合物。 本发明的方法包括（a）使样品与一定量的具有疏水性表面的官能化磁性颗粒接触，（b）将样品和颗粒温育，从而将化合物吸附到疏水表面，（c）将颗粒分离 施加磁场并除去液体，（d）任选地洗涤颗粒，（e）从颗粒中洗脱化合物。

    Resorufin derivatives
    38.
    发明授权
    Resorufin derivatives 失效
    Resorufin衍生物

    公开(公告)号：US4954630A

    公开(公告)日：1990-09-04

    申请号：US889676

    申请日：1986-07-24

    申请人： Christian Klein ,  Hans-Georg Batz ,  Rupert Herrmann

    发明人： Christian Klein ,  Hans-Georg Batz ,  Rupert Herrmann

    IPC分类号： C07C237/22 ,  C07C279/10 ,  C07D265/38 ,  C07D413/12 ,  C07J71/00 ,  C07K1/113 ,  C07K14/00 ,  C07K14/42 ,  C07K14/575 ,  C07K14/705 ,  C07K16/00 ,  C07K19/00 ,  C09B19/00 ,  G01N33/533

    CPC分类号： G01N33/533 ,  C07D265/38 ,  Y10S436/80 ,  Y10S530/802

    摘要： The present invention provides resorufin derivatives of the general formulae: ##STR1## wherein R.sup.1, R.sup.2, R.sup.3, R.sup.4 and R.sup.5, which can be the same or different, are hydrogen, halogen, carboxyl, carboxamido, lower alkoxycarbonyl, cyano or nitro groups or lower alkyl or lower alkoxy radicals, which can be substituted by carboxyl, carboxamido, lower alkoxycarbonyl, cyano or nitro groups, and wherein R.sup.4 and R.sup.5 can together also represent an anellated aromatic residue, Z is a bridge member, A is the residue of a ligand and n is a whole number of from 1 to 200.The present invention also provides processes for the preparation of these resorufin derivatives, as well as intermediates for the preparation thereof.

    摘要翻译： 本发明提供通式为：其中R 1，R 2，R 3，R 4和R 5可以相同或不同的氢，卤素，羧基，甲酰胺基，低级烷氧基羰基，氰基或硝基的通式 低级烷基或低级烷氧基，其可以被羧基，甲酰氨基，低级烷氧基羰基，氰基或硝基取代，并且其中R 4和R 5一起也可以代表苯并芳族残基，Z是桥连，A是 配体，n是1至200的整数。本发明还提供制备这些稀释剂衍生物的方法，以及制备它们的中间体。

    MEASUREMENT OF VITAMIN D
    40.
    发明申请
    MEASUREMENT OF VITAMIN D 有权
    维生素D的测定

    公开(公告)号：US20100285603A1

    公开(公告)日：2010-11-11

    申请号：US12326972

    申请日：2008-12-03

    申请人： Uwe Kobold ,  Thomas Duelffer ,  Michael Grol ,  Rupert Herrmann ,  Herbert Von der Eltz ,  Leopold Von Proff

    发明人： Uwe Kobold ,  Thomas Duelffer ,  Michael Grol ,  Rupert Herrmann ,  Herbert Von der Eltz ,  Leopold Von Proff

    IPC分类号： G01N33/00

    CPC分类号： G01N33/82 ,  Y10T436/20 ,  Y10T436/203332

    摘要： The present invention relates to a method of measuring a vitamin D metabolite in a sample, the method comprising the steps of (a) treating said sample with a vitamin D metabolite releasing reagent under conditions appropriate to release a vitamin D metabolite from vitamin D-binding protein and not to cause protein precipitation, (b) subjecting the treated sample obtained in step (a) to a chromatographic separation, and (c) measuring a vitamin D metabolite during or after said chromatographic separation. The present invention also relates to methods for determining the vitamin D status of a subject, for use in the diagnosis of disease, and to agents and kits for use in performing the methods of the invention.

    摘要翻译： 本发明涉及一种测量样品中维生素D代谢物的方法，所述方法包括以下步骤：（a）在适于从维生素D结合物释放维生素D代谢物的条件下用维生素D代谢物释放试剂处理所述样品 蛋白质，不会引起蛋白质沉淀，（b）使步骤（a）中得到的处理样品进行色谱分离，和（c）在所述色谱分离期间或之后测量维生素D代谢物。 本发明还涉及确定用于疾病诊断的受试者的维生素D状态的方法以及用于实施本发明的方法的试剂和试剂盒。