公开(公告)号：US20070054305A1

公开(公告)日：2007-03-08

申请号：US11590383

申请日：2006-10-31

申请人： Francis Barany ,  Matthew Lubin ,  George Barany ,  Robert Hammer

发明人： Francis Barany ,  Matthew Lubin ,  George Barany ,  Robert Hammer

IPC分类号： C12Q1/68 ,  C12P19/34

CPC分类号： C12Q1/6876 ,  C12Q1/6813 ,  C12Q1/6827 ,  C12Q1/683 ,  C12Q1/6851 ,  C12Q1/6853 ,  C12Q1/6858 ,  C12Q1/686 ,  C12Q1/6862 ,  C12Q1/6869 ,  C12Q1/6881 ,  C12Q2600/156 ,  C12Q2600/16 ,  C12Q2561/125 ,  C12Q2531/113 ,  C12Q2525/161 ,  C12Q2545/114 ,  C12Q2535/131 ,  C12Q2521/501 ,  C12Q2537/143 ,  C12Q2533/107 ,  C12Q2525/131 ,  C12Q2525/125 ,  C12Q2521/319 ,  C12Q2521/531 ,  C12Q2525/155

摘要： The present invention relates to a method for identifying a target nucleotide sequence. This method involves forming a ligation product on a target nucleotide sequence in a ligase detection reaction mixture, amplifying the ligation product to form an amplified ligation product in a polymerase chain reaction (PCR) mixture, detecting the amplified ligation product, and identifying the target nucleotide sequence. Such coupling of the ligase detection reaction and the polymerase chain reaction permits multiplex detection of nucleic acid sequence differences.

公开(公告)号：US07166434B2

公开(公告)日：2007-01-23

申请号：US10843720

申请日：2004-05-12

申请人： Francis Barany ,  Matthew Lubin ,  George Barany ,  Robert P. Hammer

发明人： Francis Barany ,  Matthew Lubin ,  George Barany ,  Robert P. Hammer

IPC分类号： C12Q1/68 ,  C12P19/34

CPC分类号： C12Q1/6876 ,  C12Q1/6813 ,  C12Q1/6827 ,  C12Q1/683 ,  C12Q1/6851 ,  C12Q1/6853 ,  C12Q1/6858 ,  C12Q1/686 ,  C12Q1/6862 ,  C12Q1/6869 ,  C12Q1/6881 ,  C12Q2600/156 ,  C12Q2600/16 ,  C12Q2561/125 ,  C12Q2531/113 ,  C12Q2525/161 ,  C12Q2545/114 ,  C12Q2535/131 ,  C12Q2521/501 ,  C12Q2537/143 ,  C12Q2533/107 ,  C12Q2525/131 ,  C12Q2525/125 ,  C12Q2521/319 ,  C12Q2521/531 ,  C12Q2525/155

摘要： The present invention relates to the detection of nucleic acid sequence differences using coupled ligase detection reaction and polymerase chain reaction. One aspect of the present invention involves use of a ligase detection reaction coupled to a polymerase chain reaction. Another aspect of the present invention relates to the use of a primary polymerase chain reaction coupled to a secondary polymerase chain reaction coupled to a ligase detection reaction. A third aspect of the present invention involves a primary polymerase chain reaction coupled to a secondary polymerase chain reaction. Such coupling of the ligase detection reaction and the polymerase chain reaction permits multiplex detection of nucleic acid sequence differences.

摘要翻译： 本发明涉及使用偶联连接酶检测反应和聚合酶链反应检测核酸序列差异。 本发明的一个方面涉及使用与聚合酶链式反应偶联的连接酶检测反应。 本发明的另一方面涉及与连接酶检测反应偶联的二级聚合酶链反应偶联的初级聚合酶链式反应的用途。 本发明的第三方面涉及与第二聚合酶链反应偶联的初级聚合酶链反应。 连接酶检测反应和聚合酶链式反应的这种偶联允许核酸序列差异的多重检测。

公开(公告)号：US07097980B2

公开(公告)日：2006-08-29

申请号：US10852289

申请日：2004-05-24

申请人： Francis Barany ,  Matthew Lubin ,  George Barany ,  Robert P. Hammer ,  Phillip Belgrader

发明人： Francis Barany ,  Matthew Lubin ,  George Barany ,  Robert P. Hammer ,  Phillip Belgrader

IPC分类号： C12Q1/68 ,  C12P19/34

CPC分类号： C12Q1/6876 ,  C12Q1/6813 ,  C12Q1/6827 ,  C12Q1/683 ,  C12Q1/6851 ,  C12Q1/6853 ,  C12Q1/6858 ,  C12Q1/686 ,  C12Q1/6862 ,  C12Q1/6869 ,  C12Q1/6881 ,  C12Q2600/156 ,  C12Q2600/16 ,  C12Q2561/125 ,  C12Q2531/113 ,  C12Q2525/161 ,  C12Q2545/114 ,  C12Q2535/131 ,  C12Q2521/501 ,  C12Q2537/143 ,  C12Q2533/107 ,  C12Q2525/131 ,  C12Q2525/125 ,  C12Q2521/319 ,  C12Q2521/531 ,  C12Q2525/155

摘要： The present invention relates to the detection of nucleic acid sequence differences using coupled ligase detection reaction and polymerase chain reaction. One aspect of the present invention involves use of a ligase detection reaction coupled to a polymerase chain reaction. Another aspect of the present invention relates to the use of a primary polymerase chain reaction coupled to a secondary polymerase chain reaction coupled to a ligase detection reaction. A third aspect of the present invention involves a primary polymerase chain reaction coupled to a secondary polymerase chain reaction. Such coupling of the ligase detection reaction and the polymerase chain reaction permits multiplex detection of nucleic acid sequence differences.

公开(公告)号：US20060183149A1

公开(公告)日：2006-08-17

申请号：US11369118

申请日：2006-03-06

申请人： Francis Barany ,  George Barany ,  Robert Hammer

发明人： Francis Barany ,  George Barany ,  Robert Hammer

IPC分类号： C12Q1/68 ,  C40B40/08

CPC分类号： C12Q1/6837 ,  B01J19/0046 ,  B01J2219/00432 ,  B01J2219/00527 ,  B01J2219/00529 ,  B01J2219/00536 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00608 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00711 ,  B01J2219/00722 ,  B01J2219/00729 ,  B82Y30/00 ,  C12Q1/6816 ,  C12Q1/6827 ,  C40B40/06 ,  C40B60/14 ,  Y02A50/58 ,  C12Q2525/107 ,  C12Q2525/117 ,  C12Q2565/501 ,  C12Q2561/125 ,  C12Q2537/143 ,  C12Q2565/514

摘要： The present invention describes a method for identifying one or more of a plurality of sequences differing by one or more single base changes, insertions, deletions, or translocations in a plurality of target nucleotide sequences. The method includes a ligation phase, a capture phase, and a detection phase. The ligation phase utilizes a ligation detection reaction between one oligonucleotide probe, which has a target sequence-specific portion and an addressable array-specific portion, and a second oligonucleotide probe, having a target sequence-specific portion and a detectable label. After the ligation phase, the capture phase is carried out by hybridizing the ligated oligonucleotide probes to a solid support with an array of immobilized capture oligonucleotides at least some of which are complementary to the addressable array-specific portion. Following completion of the capture phase, a detection phase is carried out to detect the labels of ligated oligonucleotide probes hybridized to the solid support. The ligation phase can be preceded by an amplification process. The present invention also relates to a kit for practicing this method, a method of forming arrays on solid supports, and the supports themselves.

公开(公告)号：US6027889A

公开(公告)日：2000-02-22

申请号：US864473

申请日：1997-05-28

申请人： Francis Barany ,  Matthew Lubin

发明人： Francis Barany ,  Matthew Lubin

IPC分类号： C12N15/09 ,  C12Q1/68 ,  C12P19/34

CPC分类号： C12Q1/6876 ,  C12Q1/6813 ,  C12Q1/6827 ,  C12Q1/683 ,  C12Q1/6851 ,  C12Q1/6853 ,  C12Q1/6858 ,  C12Q1/686 ,  C12Q1/6862 ,  C12Q1/6869 ,  C12Q1/6881 ,  C12Q2600/156 ,  C12Q2600/16

摘要： The present invention relates to the detection of nucleic acid sequence differences using coupled ligase detection reaction and polymerase chain reaction. One aspect of the present invention involves use of a ligase detection reaction coupled to a polymerase chain reaction. Another aspect of the present invention relates to the use of a primary polymerase chain reaction coupled to a secondary polymerase chain reaction coupled to a ligase detection reaction. A third aspect of the present invention involves a primary polymerase chain reaction coupled to a secondary polymerase chain reaction. Such coupling of the ligase detection reaction and the polymerase chain reaction permits multiplex detection of nucleic acid sequence differences.

公开(公告)号：US09771345B2

公开(公告)日：2017-09-26

申请号：US13500857

申请日：2010-10-07

申请人： Francis Barany ,  Maneesh Pingle ,  Sarah Filippa Giardina ,  Donald Bergstrom ,  Lee Daniel Arnold

发明人： Francis Barany ,  Maneesh Pingle ,  Sarah Filippa Giardina ,  Donald Bergstrom ,  Lee Daniel Arnold

IPC分类号： A61K31/69 ,  C07D319/14 ,  C07D207/12 ,  C07D207/24 ,  C07D209/52 ,  C07D239/42 ,  C07D239/54 ,  C07D241/36 ,  C07D263/20 ,  C07D295/18 ,  C07D307/80 ,  C07D317/10 ,  C07D317/44 ,  C07D319/12 ,  C07D403/06 ,  C07D403/14 ,  C07D405/14 ,  C07D413/06 ,  C07D471/14 ,  C07D491/04 ,  C07D491/10 ,  C07D491/14 ,  C07F5/02 ,  C40B30/04

CPC分类号： C07D319/14 ,  C07D207/12 ,  C07D207/24 ,  C07D209/52 ,  C07D239/42 ,  C07D239/54 ,  C07D241/36 ,  C07D263/20 ,  C07D295/18 ,  C07D307/80 ,  C07D317/10 ,  C07D317/44 ,  C07D319/12 ,  C07D403/06 ,  C07D403/14 ,  C07D405/14 ,  C07D413/06 ,  C07D471/14 ,  C07D491/04 ,  C07D491/10 ,  C07D491/14 ,  C07F5/025 ,  C40B30/04

摘要： The present invention is directed to a monomer useful in preparing therapeutic compounds. The monomer includes one or more pharmacophores which potentially binds to a target molecule with a dissociation constant of less than 300 μM and a linker element connected to the pharmacophore. The linker element has a molecular weight less than 500 daltons, is connected, directly or indirectly through a connector, to the pharmacophore.

公开(公告)号：US08853185B2

公开(公告)日：2014-10-07

申请号：US12937053

申请日：2009-04-09

申请人： Francis Barany ,  Maneesh Pingle ,  Donald Bergstrom ,  Sarah Filippa Giardina

发明人： Francis Barany ,  Maneesh Pingle ,  Donald Bergstrom ,  Sarah Filippa Giardina

IPC分类号： A61K31/69 ,  C07F5/02 ,  C40B30/04 ,  C40B50/04

CPC分类号： A61K47/545 ,  A61K31/69 ,  C07D233/64 ,  C07D307/92 ,  C07D309/30 ,  C07D319/24 ,  C07D493/22 ,  C07F5/02 ,  C07H7/02 ,  C07H9/02 ,  C40B30/04

摘要： A monomer useful in prepaπng therapeutic compounds includes a diversity element which potentially binds to a target molecule with a dissociation constant of less than 300 11 M and a linker element connected to the diversity element The linker element has a molecular weight less than 500 daltons, is connected, directly or indirectly through a connector, to said diversity element, and is capable of forming a reversible covalent bond or noncovalent interaction with a binding partner of the linker element The monomers can be covalently or non-covalently linked together to form a therapeutic multimer or a precursor thereof.

摘要翻译： 可用于制备治疗化合物的单体包括潜在地结合靶分子的多样性元件，其解离常数小于300±11M，连接元件连接到多样性元件。连接体元件具有小于500道尔顿的分子量 通过连接器直接或间接连接到所述多样性元件，并且能够与连接体元件的结合配偶体形成可逆共价键或非共价相互作用。所述单体可以共价或非共价连接在一起形成 治疗性多聚体或其前体。

公开(公告)号：US08597891B2

公开(公告)日：2013-12-03

申请号：US13543365

申请日：2012-07-06

申请人： Francis Barany ,  Matthew Lubin ,  George Barany ,  Robert P. Hammer

发明人： Francis Barany ,  Matthew Lubin ,  George Barany ,  Robert P. Hammer

IPC分类号： C12Q1/68 ,  C12P19/34

CPC分类号： C12Q1/6876 ,  C12Q1/6813 ,  C12Q1/6827 ,  C12Q1/683 ,  C12Q1/6851 ,  C12Q1/6853 ,  C12Q1/6858 ,  C12Q1/686 ,  C12Q1/6862 ,  C12Q1/6869 ,  C12Q1/6881 ,  C12Q2600/156 ,  C12Q2600/16 ,  C12Q2561/125 ,  C12Q2531/113 ,  C12Q2525/161 ,  C12Q2545/114 ,  C12Q2535/131 ,  C12Q2521/501 ,  C12Q2537/143 ,  C12Q2533/107 ,  C12Q2525/131 ,  C12Q2525/125 ,  C12Q2521/319 ,  C12Q2521/531 ,  C12Q2525/155

摘要： The present invention relates to a method for identifying a target nucleotide sequence. This method involves forming a ligation product on a target nucleotide sequence in a ligation detection reaction mixture, amplifying the ligation product to form an amplified ligation product in a polymerase chain reaction (PCR) mixture, detecting the amplified ligation product, and identifying the target nucleotide sequence. Such coupling of the ligase detection reaction and the polymerase chain reaction permits multiplex detection of nucleic acid sequence difference.

公开(公告)号：US08288521B2

公开(公告)日：2012-10-16

申请号：US13072442

申请日：2011-03-25

申请人： Francis Barany ,  George Barany ,  Robert P. Hammer ,  Maria Kempe ,  Herman Blok ,  Monib Zirvi

发明人： Francis Barany ,  George Barany ,  Robert P. Hammer ,  Maria Kempe ,  Herman Blok ,  Monib Zirvi

IPC分类号： C07H21/04 ,  C12N15/11

CPC分类号： C12Q1/6827 ,  B01J19/0046 ,  B01J2219/00432 ,  B01J2219/00527 ,  B01J2219/00529 ,  B01J2219/00536 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00608 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00711 ,  B01J2219/00722 ,  B01J2219/00729 ,  B82Y30/00 ,  C12Q1/6816 ,  C12Q1/6837 ,  C12Q1/6876 ,  C12Q2600/156 ,  C40B40/06 ,  C40B60/14 ,  Y02A50/58 ,  C12Q2565/501 ,  C12Q2561/125 ,  C12Q2537/143 ,  C12Q2565/514

摘要： The present invention describes a method for identifying one or more of a plurality of sequences differing by one or more single base changes, insertions, deletions, or translocations in a plurality of target nucleotide sequences. The method includes a ligation phase, a capture phase, and a detection phase. The ligation phase utilizes a ligation detection reaction between one oligonucleotide probe, which has a target sequence-specific portion and an addressable array-specific portion, and a second oligonucleotide probe, having a target sequence-specific portion and a detectable label. After the ligation phase, the capture phase is carried out by hybridizing the ligated oligonucleotide probes to a solid support with an array of immobilized capture oligonucleotides at least some of which are complementary to the addressable array-specific portion. Following completion of the capture phase, a detection phase is carried out to detect the labels of ligated oligonucleotide probes hybridized to the solid support.

摘要翻译： 本发明描述了用于鉴定多个靶核苷酸序列中一个或多个单碱基变化，插入，缺失或易位不同的多个序列中的一个或多个的方法。 该方法包括连接阶段，捕获阶段和检测阶段。 连接阶段利用具有目标序列特异性部分的一个寡核苷酸探针与可寻址阵列特异性部分之间的连接检测反应和具有靶序列特异性部分和可检测标记的第二寡核苷酸探针。 在连接阶段之后，捕获阶段通过将连接的寡核苷酸探针与具有固定化捕获寡核苷酸阵列的固体支持物杂交进行，其中至少一些与可寻址阵列特异性部分互补。 捕获阶段完成后，进行检测阶段以检测与固体支持物杂交的连接的寡核苷酸探针的标记。

公开(公告)号：US20110136116A1

公开(公告)日：2011-06-09

申请号：US12934866

申请日：2009-04-08

申请人： Francis Barany ,  Maneesh Pingle ,  Donald Bergstrom

发明人： Francis Barany ,  Maneesh Pingle ,  Donald Bergstrom

IPC分类号： C12Q1/68 ,  G01N33/53

CPC分类号： C12Q1/6827 ,  Y10T436/143333 ,  C12Q2561/125 ,  C12Q2527/107 ,  C12Q2525/301

摘要： A method for identifying a plurality of target nucleic acid molecules in a sample. The method provides a plurality of oligonucleotide probe sets. Each set comprises a first and a second probe, each having a target-specific portion and a tunable portion with an acceptor or a donor group. The first probe further comprises an endcapped hairpin. A reaction comprises a denaturation and hybridization cycle. Under the hybridization, the set of probes hybridize in a base-specific manner to their respective target nucleotide sequences, and ligate to one another to form a ligation product. Under conditions that permit hybridization of the tunable portions of the ligation product to one another, an internally hybridized ligation product formed, which allows the detection of the fluorescence resonance energy transfer (FRET). A method comprising PCR amplification is also disclosed.

摘要翻译： 用于鉴定样品中多个靶核酸分子的方法。 该方法提供多个寡核苷酸探针组。 每组包括第一和第二探针，每个具有目标特异性部分和具有受体或供体基团的可调部分。 第一探针还包含封端的发夹。 反应包括变性和杂交循环。 在杂交下，探针组以碱基特异性方式与其各自的靶核苷酸序列杂交，并且彼此连接以形成连接产物。 在允许连接产物的可调部分彼此杂交的条件下，形成内部杂交的连接产物，其允许检测荧光共振能量转移（FRET）。 还公开了包括PCR扩增的方法。