Enzyme with .beta.-(1-6)- endoglucanase activity
    31.
    发明授权
    Enzyme with .beta.-(1-6)- endoglucanase activity 失效
    具有β-(1-6) - 内切葡聚糖酶活性的酶

    公开(公告)号:US6022723A

    公开(公告)日:2000-02-08

    申请号:US40699

    申请日:1998-03-18

    摘要: The present invention relates to an enzyme with .beta.-(1-6)-endoglucanase activity encoded by a DNA sequence, which DNA sequence a) comprises the DNA sequence shown in SEQ ID No. 3, or b) comprises an analogue of the DNA sequence shown in SEQ ID No. 3, which i) is homologous with the DNA sequences shown in or SEQ ID No. 3, and/or ii) hybridizes with the same oligonucleotide probe as the DNA sequence shown in SEQ ID No. 3, and/or iii) encodes a polypeptide which is homologous with the polypeptide encoded by a DNA sequence comprising the DNA sequence shown in SEQ ID No. 3, and/or iv) encodes a polypeptide which is immunologically reactive with an antibody raised against a purified .beta.-(1-6)-glucanase shown in SEQ ID No. 4 derived from Trichoderma harzianum, CBS 243.71. The present invention also relates to a DNA construct encoding the enzyme, a method of producing the enzyme, an enzyme preparation containing the enzyme, and the use of the enzyme and enzyme preparation for the degradation or modification of .beta.-glucan containing materials.

    摘要翻译: 本发明涉及由DNA序列编码的β-(1-6) - 内葡聚糖酶活性的酶,该DNA序列a)包含SEQ ID No.3所示的DNA序列,或b)包含DNA的类似物 序列号3所示的序列,其i)与SEQ ID No.3所示的DNA序列同源,和/或ii)与与SEQ ID No.3所示的DNA序列相同的寡核苷酸探针杂交, 和/或iii)编码与由包含SEQ ID No.3所示的DNA序列的DNA序列编码的多肽同源的多肽,和/或iv)编码与针对纯化的抗体产生的抗体具有免疫反应性的多肽 β-(1-6) - 葡聚糖酶,如SEQ ID No.4所示,源自哈茨木霉,CBS 243.71。 本发明还涉及编码该酶的DNA构建体,该酶的制备方法,含该酶的酶制剂,以及该酶和酶制剂用于含β-葡聚糖的材料的降解或修饰的用途。

    Transglutaminase from oomycetes
    33.
    发明授权
    Transglutaminase from oomycetes 失效
    来自卵菌的转谷氨酰胺酶

    公开(公告)号:US07094586B2

    公开(公告)日:2006-08-22

    申请号:US10164765

    申请日:2002-06-07

    IPC分类号: C12N9/10 C12N15/54

    摘要: The present invention relates to transglutaminases and transglutaminase preparations obtained from lower fungi belonging to the class Oomycetes and unprecedented high-level expression is achievable by growing these coenocytium forming organisms, especially the strains Pythium sp., Pythium irregulare, Pythium dissotocum, Pythium periilum (or P. periplocum), Pythium torulosum, Pythium ultimum, Pythium aphanidermatum, Phytophthora cactorum, Phytophthora palmivora, Phytophthora porri, Phytophthora infestans, Phytophthora megasperma, Phytophthora cinnamomi and Phytophthora cryptogea; and a recombinant transglutaminase has been cloned and expressed, the enzyme and enzyme preparations being useful for cross-linking proteins, e.g. in flour, baked products, meat products, fish products, cosmetics, cheese, milk products, gelled food products and leather finishing, or as a glutaminase, e.g. in bread and other baked glutein-containing food products.

    摘要翻译: 本发明涉及从属于卵菌纲的低级真菌得到的转谷氨酰胺酶和转谷氨酰胺酶制剂,并且具有前所未有的高水平表达是通过生长这些形成细胞的生物体,特别是菌株腐霉菌,非腐霉菌,不锈菌腐霉,腐霉腐霉(或 P.腐生菌),腐皮腐,腐霉,腐霉,腐皮疫霉,疫霉疫霉,致病疫霉,致热疫霉,肉,疫霉和疫霉疫霉;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;; 并且克隆和表达重组转谷氨酰胺酶,所述酶和酶制剂可用于交联蛋白质,例如, 在面粉,烘焙食品,肉制品,鱼制品,化妆品,奶酪,乳制品,凝胶食品和皮革整理中,或作为谷氨酰胺酶。 在面包和其他烘烤含麸质的食品中。

    Enzyme with endo-1,3(4)-.beta.-glucanase activity
    34.
    发明授权
    Enzyme with endo-1,3(4)-.beta.-glucanase activity 失效
    具有内切-1,3(4)-β-葡聚糖酶活性的酶

    公开(公告)号:US6140096A

    公开(公告)日:2000-10-31

    申请号:US98580

    申请日:1998-06-17

    摘要: An enzyme having endo-1,3(4)-.beta.-glucanase activity is described which is encoded by the DNA sequence ATGTGGTCTCCCAAGGTTGCTGCTGCCGTCCTCGCCTTTGTTGGTGCTACCAACGCCT GGCAGCCCCCGACCTACAGCGGCTTCAACTTGGTCTGGACTGACACCTTCGCTGGCAACGGTGGCACTTCTCCTA ACCAGAACAACTGGAACATCATCACCGGAAACTTGAACGTCAACGCCGAGCAGGAGACCTACTCCTCCAGCACC GCCAATGTTCAGCTCAGTGGTGGCAGCACCCTTCAGCTGGTCCCCTGGAGAGACAGCAGCAAGGGAACCAGCAC CTTTGGTGGCTGGACCTCCGGTCGTCTTGAGTCCAAGTACACATTCACTCCCGCGGCCGGCAAGGTCACCCGTCTT GAAGCCGCCATCCGCTTCGGCAGCAACGCTCAGGCCAACAAGCAGGGTATCTGGCCTGCTTTCTGGATGCTGGGT GACTCCCTCCGTCAACCGGGCGGCAGCTGGCCCAACTGTGGTGAGATCGACATCATGGAGACTGTCGACGGCCA GGCTACCGGCCACGGTACCCTTCACTGCGACGTCTACCCCGGCGGTATCTGCAACGAGGGTAACGGTATTGGAGG CCCTGTCAACATCGCCAACGTCAACGACTGGCACGCTTGGCGTGTTGAGATCGACCGCACTCCCAGCAGCTGGCA ATCCGAGACCCTCACCTGGTCCCTCGACGGCACCATCTACTTCCAGATCACTGGCTCTCGCATTGGCAACCAGGG CGTCTGGAACAACATTGCTCACAGCCCCCTCTTCTTCATTCTTAACGTTGCTGTCGGTGGCAACTGGCCTGGCAAC CCCAACAGCGCTACCCTCGATGGCTACGGAAGCATGATGGAGGTTGGCTACGTCGCTCAGTACTCTACCTAA (SEQ ID NO:3).

    摘要翻译: 酶具有内切-1,3(4) - β葡聚糖酶活性被描述这是由DNA序列ATGTGGTCTCCCAAGGTTGCTGCTGCCGTCCTCGCCTTTGTTGGTGCTACCAACGCCT GGCAGCCCCCGACCTACAGCGGCTTCAACTTGGTCTGGACTGACACCTTCGCTGGCAACGGTGGCACTTCTCCTA ACCAGAACAACTGGAACATCATCACCGGAAACTTGAACGTCAACGCCGAGCAGGAGACCTACTCCTCCAGCACC GCCAATGTTCAGCTCAGTGGTGGCAGCACCCTTCAGCTGGTCCCCTGGAGAGACAGCAGCAAGGGAACCAGCAC CTTTGGTGGCTGGACCTCCGGTCGTCTTGAGTCCAAGTACACATTCACTCCCGCGGCCGGCAAGGTCACCCGTCTT GAAGCCGCCATCCGCTTCGGCAGCAACGCTCAGGCCAACAAGCAGGGTATCTGGCCTGCTTTCTGGATGCTGGGT GACTCCCTCCGTCAACCGGGCGGCAGCTGGCCCAACTGTGGTGAGATCGACATCATGGAGACTGTCGACGGCCA GGCTACCGGCCACGGTACCCTTCACTGCGACGTCTACCCCGGCGGTATCTGCAACGAGGGTAACGGTATTGGAGG CCCTGTCAACATCGCCAACGTCAACGACTGGCACGCTTGGCGTGTTGAGATCGACCGCACTCCCAGCAGCTGGCA ATCCGAGACCCTCACCTGGTCCCTCGACGGCACCATCTACTTCCAGATCACTGGCTCTCGCATTGGCAACCAGGG CGTCTGGAACAACATTGCTCACAGCCCCCTCTTCTTCATTCTTAACGTTGCTGTCGGTGGCAACTGGCCTGGCAAC CCCAACAGCGCTACCCTCGATGGCTACGGAAGCATGATGGAGGTTGGCTACGTCGCTCAGTACTCTACCTAA(SEQ编码 编号:3)。

    Xyloglucanase from Malbranchea
    37.
    发明授权
    Xyloglucanase from Malbranchea 失效
    来自马尔布兰卡的葡聚糖酶

    公开(公告)号:US06500658B1

    公开(公告)日:2002-12-31

    申请号:US09653778

    申请日:2000-09-01

    IPC分类号: C12P2106

    摘要: An isolated or purified polypeptide having xyloglucanase activity which is obtained from a strain of the genus Malbranchea and has xyloglucanase activity in the pH range 4-11, measured at 50° C.; and/or a molecular mass of 25±10 kDa, as determined by SDS-PAGE; and/or an isoelectric point (pI) in the range 3-5; and/or an N-terminal sequence Ala-Asp-Phe-Cys-Gly-Gln-Xaa-Asp-Ser-Glu-Gln-Ser-Gly-Pro-Tyr-Ile-Val-Tyr-Asn-Asn-Leu is useful in industrial applications such as in laundry detergent compositions and for treatment of textiles.

    摘要翻译: 具有木葡聚糖酶活性的分离的或纯化的多肽,其从马氏体属的菌株获得,并且在50℃下测量的pH范围4-11中具有木葡聚糖酶活性。 和/或25±10kDa的分子量,通过SDS-PAGE测定; 和/或3-5的等电点(pI); 和/或N-末端序列Ala-Asp-Phe-Cys-Gly-Gln-Xaa-Asp-Ser-Glu-Gln-Ser-Gly-Pro-Tyr-Ile-Val-Tyr-Asn-Asn-Leu是 可用于工业应用,例如洗衣洗涤剂组合物和用于纺织品的处理。

    Enzymes with aminopeptidase activity
    39.
    发明授权
    Enzymes with aminopeptidase activity 失效
    具有氨基肽酶活性的酶

    公开(公告)号:US06413559B1

    公开(公告)日:2002-07-02

    申请号:US09718709

    申请日:2000-11-22

    IPC分类号: A21D200

    CPC分类号: C12N9/48 A21D8/042

    摘要: The present invention relates to a 35 kDa enzyme exhibiting aminopeptidase activity which is derived from a fungal microorganism, a DNA construct comprising a DNA sequence encoding said enzyme, a recombinant expression vector comprising said DNA construct, and a cell comprising said DNA sequence. The present invention also provides a method for producing said enzyme exhibiting aminopeptidase activity, and an enzyme preparation comprising said enzyme, a bread-improving or dough-improving composition comprising the aminopeptidase of the invention. Finally the invention relates to the use of said enzyme exhibiting aminopeptidase activity or enzyme preparations or compositions thereof.

    摘要翻译: 本发明涉及一种表现出来自真菌微生物的氨基肽酶活性的35kDa酶,包含编码所述酶的DNA序列的DNA构建体,包含所述DNA构建体的重组表达载体和包含所述DNA序列的细胞。 本发明还提供了生产表现出氨基肽酶活性的酶的方法,以及包含所述酶的酶制剂,包含本发明的氨基肽酶的面包改良组合物或面团改良组合物。 最后,本发明涉及所述显示氨基肽酶活性的酶或酶制剂或其组合物的用途。