公开(公告)号：US09920341B2

公开(公告)日：2018-03-20

申请号：US15454544

申请日：2017-03-09

申请人： Novozymes A/S

发明人： Sara Landvik ,  Lars Henrik Oestergaard ,  Lisbeth Kalum

IPC分类号： C12P7/04 ,  C12P7/02 ,  C12N9/08 ,  C12N15/00 ,  C12P7/66 ,  C12P7/22 ,  C12P7/00 ,  C12P7/26 ,  C12P7/24 ,  C12P7/40

CPC分类号： C12P7/66 ,  C11D3/38654 ,  C12N9/0065 ,  C12P7/00 ,  C12P7/04 ,  C12P7/18 ,  C12P7/22 ,  C12P7/24 ,  C12P7/26 ,  C12P7/40 ,  C12P7/64 ,  C12Y111/02001 ,  Y02P20/52

摘要： The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

公开(公告)号：US09920334B2

公开(公告)日：2018-03-20

申请号：US15009453

申请日：2016-01-28

申请人： Evonik Degussa GmbH

发明人： Thomas Haas ,  Thomas Buelter ,  Martin Demler ,  Simon Beck

IPC分类号： C12N1/14 ,  C12P7/06 ,  C12N1/20 ,  C12P7/02 ,  C12R1/145 ,  C12P7/04 ,  C12P7/14

CPC分类号： C12P7/065 ,  C12N1/20 ,  C12P7/02 ,  C12P7/04 ,  C12P7/14 ,  C12R1/145 ,  Y02E50/17

摘要： A reaction mixture for producing ethanol and/or acetate from a carbon source in aerobic conditions, wherein the mixture comprises a first acetogenic microorganism in an exponential growth phase; free oxygen; and a second acetogenic microorganism in a stationary phase wherein the first and second acetogenic microorganism is capable of converting the carbon source to the acetate and/or ethanol.

公开(公告)号：US09919282B2

公开(公告)日：2018-03-20

申请号：US15181319

申请日：2016-06-13

申请人： Xyleco, Inc.

发明人： Marshall Medoff

IPC分类号： B01J19/08 ,  C10G3/00 ,  C10G11/00 ,  C10G15/10 ,  C10G47/00 ,  C10L1/04 ,  C12P19/14 ,  C12P7/14 ,  C08B1/00 ,  C12P7/10 ,  C12P19/02 ,  C10L1/02 ,  C12P5/00 ,  C12P7/04 ,  C12P7/16 ,  H01M8/22 ,  H01M8/1007 ,  H01M8/1011 ,  C12P5/02 ,  C12P7/02 ,  H01M8/16

CPC分类号： B01J19/08 ,  B01J19/081 ,  B01J19/085 ,  B01J2219/08 ,  B01J2219/0879 ,  C08B1/00 ,  C10G3/00 ,  C10G11/00 ,  C10G15/10 ,  C10G47/00 ,  C10G2300/1011 ,  C10L1/02 ,  C10L1/04 ,  C10L2200/0469 ,  C10L2270/02 ,  C10L2270/04 ,  C10L2290/02 ,  C10L2290/06 ,  C10L2290/26 ,  C10L2290/34 ,  C10L2290/36 ,  C10L2290/543 ,  C12P5/00 ,  C12P5/02 ,  C12P5/023 ,  C12P7/02 ,  C12P7/04 ,  C12P7/10 ,  C12P7/14 ,  C12P7/16 ,  C12P19/02 ,  C12P19/14 ,  C12P2201/00 ,  C12P2203/00 ,  H01M8/1007 ,  H01M8/1013 ,  H01M8/16 ,  H01M8/22 ,  Y02E50/16 ,  Y02E50/343 ,  Y02P30/20 ,  Y02T50/678

摘要： Biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) is processed to produce useful products, such as fuels. For example, systems can use feedstock materials, such as cellulosic and/or lignocellulosic materials and/or starchy materials, to produce ethanol and/or butanol, e.g., by fermentation.

公开(公告)号：US09909147B2

公开(公告)日：2018-03-06

申请号：US15349024

申请日：2016-11-11

申请人： Novozymes A/S

发明人： Martin Hofrichter ,  Katrin Scheibner ,  Rene Ullrich ,  Matthias Kinne ,  Sebastian Peter ,  Henrik Lund ,  Lisbeth Kalum

IPC分类号： C12P7/04 ,  C12P7/02 ,  C12P7/42 ,  C12P7/00 ,  C12N9/02

CPC分类号： C12P7/04 ,  C12N9/0071 ,  C12P7/00 ,  C12P7/02 ,  C12P7/42 ,  C12Y111/02001 ,  C12Y114/14001

摘要： The invention relates to enzymatic methods for hydroxylation in position 2 or 3 of substituted or unsubstituted, linear or branched aliphatic hydrocarbons.

公开(公告)号：US09890401B2

公开(公告)日：2018-02-13

申请号：US14720240

申请日：2015-05-22

申请人： REG Life Sciences, LLC

发明人： Zhihao Hu ,  Vikranth Arlagadda

IPC分类号： C12N1/20 ,  C12N9/02 ,  C12P7/64 ,  C12P7/04 ,  A61K38/00 ,  A61K31/045 ,  C12R1/01

CPC分类号： C12P7/64 ,  A61K31/045 ,  C12N1/20 ,  C12N9/0004 ,  C12N2501/71 ,  C12N2502/99 ,  C12P7/04 ,  C12R1/01 ,  Y02A50/473

摘要： Methods and compositions, including nucleotide sequences, amino acid sequences, and host cells, for producing fatty alcohols are described.

公开(公告)号：US20180010137A1

公开(公告)日：2018-01-11

申请号：US15650285

申请日：2017-07-14

申请人： REG Life Sciences, LLC

发明人： Derek L. GREENFIELD ,  Andreas W. Schirmer ,  Elizabeth J. CLARKE ,  Eli S. GROBAN ,  Bernardo M. Da Costa ,  Zhihao Hu ,  Kevin Holden ,  Noah Helman

IPC分类号： C12N15/70 ,  C12P7/64 ,  C12P7/04 ,  C12N9/02 ,  C12N9/90 ,  C12N9/88 ,  C12N9/12 ,  C12N9/10 ,  C12N9/04 ,  C12N9/00

CPC分类号： C12N15/70 ,  C12N9/0006 ,  C12N9/001 ,  C12N9/1029 ,  C12N9/1288 ,  C12N9/88 ,  C12N9/90 ,  C12N9/93 ,  C12P7/04 ,  C12P7/6409 ,  C12P7/6436 ,  C12Y101/011 ,  C12Y103/01009 ,  C12Y103/0101 ,  C12Y203/01039 ,  C12Y203/01041 ,  C12Y203/01179 ,  C12Y203/0118 ,  C12Y207/08007 ,  C12Y402/01059 ,  C12Y503/03014 ,  C12Y604/01002

摘要： The invention relates to compositions and methods, including polynucleotide sequences, amino acid sequences, recombinant host cells and recombinant host cell cultures engineered to produce fatty acid derivative compositions comprising fatty acids, fatty alcohols, fatty aldehydes, fatty esters, alkanes, terminal olefins, internal olefins or ketones. The fatty acid derivative composition is produced extracellularly with a higher titer, yield or productivity than the corresponding wild type or non-engineered host cell.

公开(公告)号：US20170369860A1

公开(公告)日：2017-12-28

申请号：US15538816

申请日：2016-01-06

申请人： Novozymes A/S

发明人： Robert Blazej ,  Charles Emrich ,  Nicholas Toriello ,  Hanshu Ding ,  David Osborn ,  Aubrey Jones

IPC分类号： C12N9/42 ,  C12P19/14 ,  C12P5/02 ,  C12P5/00 ,  D21C5/00 ,  C12P7/40 ,  C12P3/00

CPC分类号： C12N9/2437 ,  C12P3/00 ,  C12P5/007 ,  C12P5/02 ,  C12P5/023 ,  C12P5/026 ,  C12P7/04 ,  C12P7/40 ,  C12P19/14 ,  C12Y302/01004 ,  D21C5/005 ,  D21H17/005 ,  Y02E50/343

摘要： The present invention relates to endoglucanase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, expression vectors, and recombinant host cells comprising the polynucleotides; and methods of using the variants.

公开(公告)号：US09850504B2

公开(公告)日：2017-12-26

申请号：US14902565

申请日：2014-07-02

申请人： Scientist of Fortune S.A. ,  Global Bioenergies

发明人： Philippe Marliere ,  Maria Anissimova ,  Mathieu Allard

IPC分类号： C12N9/88 ,  C12P7/16 ,  C12N1/00 ,  C12P5/02 ,  C12P7/26 ,  C12N9/04 ,  C12N9/02 ,  C12N15/52 ,  C12P7/04 ,  C12N15/70 ,  C12P19/32

CPC分类号： C12P7/16 ,  C12N1/00 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/001 ,  C12N9/88 ,  C12N15/52 ,  C12N15/70 ,  C12P5/026 ,  C12P7/04 ,  C12P7/26 ,  C12P19/32 ,  C12Y101/01001 ,  C12Y101/0103 ,  C12Y101/01034 ,  C12Y101/01184 ,  C12Y102/0105 ,  C12Y103/08001 ,  C12Y402/01017 ,  C12Y402/01018 ,  C12Y402/01055 ,  C12Y402/01059 ,  C12Y402/01074 ,  C12Y402/01116 ,  C12Y402/01127 ,  Y02E50/10

摘要： Described is a method for the production of 3-buten-2-one comprising the enzymatic conversion of 4-hydroxy-2-butanone into 3-buten-2-one by making use of an enzyme catalyzing 4-hydroxy-2-butanone dehydration, wherein said enzyme catalyzing 4-hydroxy-2-butanone dehydration is (a) a 3-hydroxypropiony-CoA dehydratase (EC 4.2.1.116), (b) a 3-hydroxybutyryl-CoA dehydratase (EC 4.2.1.55), (c) an enoyl-CoA hydratase (EC 4.2.1.17), (d) a 3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase (EC 4.2.1.59), (e) a crotonyl-[acyl-carrier-protein] hydratase (EC 4.2.1.58), (f) a 3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase (EC 4.2.1.60), (g) a 3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase (EC 4.2.1.61), (h) a long-chain-enoyl-CoA hydratase (EC 4.2.1.74), or (i) a 3-methylglutaconyl-CoA hydratase (EC 4.2.1.18). The produced 3-buten-2-one can be further converted into 3-buten-2-ol and finally into 1,3-butadiene.

公开(公告)号：US09834795B2

公开(公告)日：2017-12-05

申请号：US15205891

申请日：2016-07-08

申请人： LanzaTech New Zealand Limited

发明人： Michael Koepke ,  Sean Simpson ,  Fungmin Liew ,  Wendy Yiting Chen

IPC分类号： C12P7/30 ,  C12N15/74 ,  C12N9/10 ,  C12N9/88 ,  C12N9/04 ,  C12N15/52 ,  C12P7/04 ,  C12P7/28

CPC分类号： C12P7/30 ,  C12N9/0006 ,  C12N9/1029 ,  C12N9/13 ,  C12N9/88 ,  C12N15/52 ,  C12N15/74 ,  C12P7/04 ,  C12P7/28 ,  C12Y101/01001 ,  C12Y203/01009 ,  C12Y208/03009 ,  C12Y401/01004 ,  Y02P20/52

摘要： The invention provides recombinant microorganisms and methods for the production of acetone from gaseous substrates. For example, the recombinant microorganism may be modified to express an exogenous thiolase, an exogenous CoA transferase, and an exogenous decarboxylase.

公开(公告)号：US20170342444A1

公开(公告)日：2017-11-30

申请号：US15605930

申请日：2017-05-25

申请人： API Intellectual Property Holdings, LLC

发明人： Shrikant SURVASE ,  Ryan ZEBROSKI ,  Georgios ADAMOS ,  Vesa PYLKKANEN

IPC分类号： C12P7/10 ,  C12P7/16 ,  C12P7/28 ,  C12P19/02 ,  C12P7/04 ,  C12P7/52 ,  C12P7/54 ,  C12P19/14

CPC分类号： C12P7/10 ,  C12P7/04 ,  C12P7/16 ,  C12P7/28 ,  C12P7/52 ,  C12P7/54 ,  C12P19/02 ,  C12P19/14 ,  C12P2201/00 ,  C12P2203/00 ,  Y02E50/10 ,  Y02E50/16 ,  Y02E50/17

摘要： This invention provides optimized fermentation of cellulosic and hemicellulosic sugars. Biomass-derived hemicellulosic and cellulosic sugars are independently conditioned and separately fermented, utilizing reuse and recycle of microorganisms, metabolic intermediates, and nutrients. Conditioned sugars can be fermented in separate vessels, where excess cells from glucose fermentation are conveyed to hemicellulose sugar fermentation along with raffinate from solvent recovery, to enhance productivity and product yield. Some variations provide a method of fermenting C5 and C6 sugars to fermentation products, the method comprising: fermenting a C6-rich sugar feed to a first fermentation product; fermenting a C5-rich sugar feed to a second fermentation product; removing microorganism cells from the first fermentor, to maintain a cell concentration within a selected range; conveying microorganism cells to a second fermentor; and removing microorganism cells from the second fermentor, to maintain a microorganism cell concentration that is greater than that in the C6-rich fermentor.