公开(公告)号：US20160195492A1

公开(公告)日：2016-07-07

申请号：US14987352

申请日：2016-01-04

Applicant: Advanced Liquid Logic, Inc.

Inventor： William Craig Bauer

IPC: G01N27/447 ,  B01L7/00 ,  B01L3/00

CPC classification number: G01N27/44734 ,  B01F13/0071 ,  B01F13/0076 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502761 ,  B01L3/502784 ,  B01L7/525 ,  B01L7/54 ,  B01L2200/027 ,  B01L2200/06 ,  B01L2200/0668 ,  B01L2200/10 ,  B01L2200/16 ,  B01L2300/0887 ,  B01L2300/12 ,  B01L2300/18 ,  B01L2300/1805 ,  B01L2300/1827 ,  B01L2400/0427 ,  B01L2400/043 ,  B01L2400/0677 ,  B01L2400/0683 ,  G01N27/44791

Abstract: The invention relates to a droplet actuator device and methods for integrated sample preparation and analysis of a biological sample. A droplet actuator device is provided for conducting droplet operations. The droplet actuator device may include a bottom substrate and a top substrate separated from each other to form a gap therebetween; an arrangement of droplet operations electrodes arranged on one or both of the bottom and/or top substrates for conducting droplet operations thereon; a reagent storage layer comprising one or more compartments bound to the top substrate; and one or more openings arranged to provide a fluidic path from the one or more compartments into the gap, upon breach of a breachable seal separating the one or more compartments and openings.

公开(公告)号：US20160129437A1

公开(公告)日：2016-05-12

申请号：US14538506

申请日：2014-11-11

Applicant: GENMARK DIAGNOSTICS, INC. ,  ADVANCED LIQUID LOGIC, INC.

Inventor： JON FAIZ KAYYEM ,  SEAN FORD ,  VAMSEE K. PAMULA ,  VIJAY SRINIVASAN ,  SCOTT COREY ,  BRIAN MURPHY ,  DAVID WALTER WRIGHT ,  DOMINIC AIELLO

IPC: B01L3/00 ,  B01L7/00

CPC classification number: B01L3/502 ,  B01L3/502792 ,  B01L3/52 ,  B01L7/525 ,  B01L2200/04 ,  B01L2200/0647 ,  B01L2200/10 ,  B01L2200/16 ,  B01L2300/021 ,  B01L2300/04 ,  B01L2300/06 ,  B01L2300/0636 ,  B01L2300/0672 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2300/0874 ,  B01L2300/123 ,  B01L2300/1822 ,  B01L2400/0427 ,  B01L2400/043 ,  B01L2400/0481 ,  B01L2400/0487 ,  B01L2400/0683

Abstract: In one embodiment, a multiplex fluid processing cartridge includes a sample well, a deformable fluid chamber, a mixing well with a mixer disposed therein, a lysis chamber including a lysis mixer, an electrowetting grid for microdroplet manipulation, and electrosensor arrays configured to detect analytes of interest. An instrument for processing the cartridge is configured to receive the cartridge and to selectively apply thermal energy, magnetic force, and electrical connections to one or more discrete locations on the cartridge and is further configured to compress the deformable chamber(s) in a specified sequence.

Abstract translation: 在一个实施例中，多重流体处理盒包括样品阱，可变形流体室，与其中设置的混合器的混合井，包括裂解混合器的裂解室，用于微滴操作的电润湿栅格，以及配置成检测分析物的电传感器阵列 出于兴趣。 用于处理盒的装置被配置为接收盒并且选择性地将热能，磁力和电连接施加到盒上的一个或多个离散位置，并且还被配置成以指定的顺序压缩可变形室 。

公开(公告)号：US20160109338A1

公开(公告)日：2016-04-21

申请号：US14978935

申请日：2015-12-22

Applicant: Advanced Liquid Logic, Inc.

Inventor： Ramakrishna Sista ,  Vamsee K. Pamula ,  Vijay Srinivasan ,  Michael G. Pollack ,  Allen Eckhardt

IPC: G01N1/38

CPC classification number: G01N33/54326 ,  B01F11/0071 ,  B01F13/0071 ,  B01F13/0076 ,  B01F13/0809 ,  B01L3/50273 ,  B01L3/502761 ,  B01L3/502792 ,  B01L7/52 ,  B01L7/525 ,  B01L2200/027 ,  B01L2200/0647 ,  B01L2200/0673 ,  B01L2300/06 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/089 ,  B01L2300/1827 ,  B01L2400/0406 ,  B01L2400/0415 ,  B01L2400/0421 ,  B01L2400/0424 ,  B01L2400/0427 ,  B01L2400/043 ,  B01L2400/0436 ,  B01L2400/0442 ,  G01N1/38 ,  G01N2001/386 ,  G01N2035/1046 ,  Y10T436/11 ,  Y10T436/25 ,  Y10T436/25625 ,  Y10T436/2575

Abstract: The invention provides a method of circulating magnetically responsive beads within a droplet in a droplet actuator. The invention also provides methods for splitting droplets. The invention, in one embodiment, makes use of a droplet actuator with top and bottom substrates, a plurality of magnetic fields respectively present proximate the top and bottom substrates, wherein at least one of the magnet fields is selectively alterable, and a plurality of droplet operations electrodes positioned along at least one of the top and bottom surfaces. A droplet is positioned between the top and bottom surfaces and at least one of the magnetic fields is selectively altered.

公开(公告)号：US20160097047A1

公开(公告)日：2016-04-07

申请号：US14969933

申请日：2015-12-15

Applicant: Advanced Liquid Logic, Inc

Inventor： Michael G. Pollack ,  Vamsee K. Pamula ,  Allen E. Eckhardt

IPC: C12N13/00 ,  G01N15/10

CPC classification number: C12N13/00 ,  B01J2219/00527 ,  B01J2219/00653 ,  B01J2219/00743 ,  G01N15/10 ,  G01N35/10 ,  G01N2015/0088 ,  G01N2015/1081 ,  G01N2035/00564 ,  G01N2035/1034 ,  Y10T436/11 ,  Y10T436/25 ,  Y10T436/2575

Abstract: A method of growing cells on a droplet actuator is provided. The method may include providing a droplet actuator including a cell culture droplet including a cell culture medium and one or more cells; and maintaining the droplet at a temperature suitable for causing the cells to grow in the cell culture medium on the droplet actuator. Related methods, droplet actuators, and systems are also provided.

Abstract translation: 提供了一种在液滴致动器上生长细胞的方法。 该方法可以包括提供包括包含细胞培养基和一个或多个细胞的细胞培养液滴的液滴致动器; 并且将液滴保持在适于使细胞在液滴致动器上的细胞培养基中生长的温度。 还提供了相关方法，液滴致动器和系统。

公开(公告)号：US20150268235A1

公开(公告)日：2015-09-24

申请号：US14731740

申请日：2015-06-05

Applicant: Advanced Liquid Logic, Inc.

Inventor： Michael G. Pollack ,  Vamsee K. Pamula ,  Ramakrishna Sista ,  Arjun Sudarsan

IPC: G01N33/543

CPC classification number: G01N33/54333 ,  B01F11/0071 ,  B01F13/0071 ,  B01F13/0076 ,  B01L3/502715 ,  B01L3/502761 ,  B01L3/502784 ,  B01L3/502792 ,  B01L7/52 ,  B01L7/525 ,  B01L2200/027 ,  B01L2200/0647 ,  B01L2200/0668 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/089 ,  B01L2300/1827 ,  B01L2400/0406 ,  B01L2400/0415 ,  B01L2400/0421 ,  B01L2400/0424 ,  B01L2400/0427 ,  B01L2400/043 ,  B01L2400/0436 ,  B01L2400/0442 ,  B03C1/02 ,  B03C1/288 ,  B03C1/30 ,  B03C5/005 ,  B03C2201/18 ,  B03C2201/26 ,  C12N15/1013 ,  G01N33/561 ,  G01N2035/1046 ,  Y10T436/11 ,  Y10T436/25 ,  Y10T436/25625 ,  Y10T436/2575

Abstract: The invention relates to bead incubating and washing on a droplet actuator. Methods for incubating magnetically responsive beads that are labeled with primary antibody, a sample (i.e., analyte), and secondary reporter antibodies on a magnet, on and off a magnet, and completely off a magnet are provided. Also provided are methods for washing magnetically responsive beads using shape-assisted merging of droplets. Also provided are methods for shape-mediated splitting, transporting, and dispensing of a sample droplet that contains magnetically responsive beads. The apparatuses and methods of the invention provide for rapid time to result and optimum detection of an analyte in an immunoassay.

Abstract translation: 本发明涉及在液滴致动器上的珠孵化和洗涤。 提供了用于将用一抗标记的磁响应珠，样品（即分析物）和二次报告基因抗体孵育在磁体上，磁铁上和从磁体上离开并完全离开磁体的方法。 还提供了使用形状辅助的液滴合并来洗涤磁响应珠的方法。 还提供了用于形状介导的分离，输送和分配含有磁响应珠的样品液滴的方法。 本发明的装置和方法提供了免疫测定中分析物的快速结果和最佳检测的时间。

公开(公告)号：US20150267242A1

公开(公告)日：2015-09-24

申请号：US14439255

申请日：2013-10-30

Applicant: ADVANCED LIQUID LOGIC, INC

Inventor： Nora J. Foegeding ,  Carrie A. Graham ,  Allen E. Eckhardt

IPC: C12Q1/32

CPC classification number: C12Q1/32 ,  G01N33/6893 ,  G01N2333/90206 ,  G01N2800/04 ,  G01N2800/7085

Abstract: A method for conducting medium-chain acyl-CoA dehydrogenase (MCAD) and very-long-chain acyl-CoA dehydrogenase (VCAD) enzymatic activity assays is provided. The method may include, but is not limited to, preparing a sample; preparing an enzyme-specific substrate/reagent mixture; mixing an aliquot of the prepared sample with an aliquot of the enzyme-specific substrate/reagent mixture; reading absorbance in the range of about 600 nm; incubating the prepared sample and enzyme-specific substrate/reagent mixture; and reading absorbance in the range of about 600 nm at various time intervals.

Abstract translation: 提供了一种用于进行中链酰基辅酶A脱氢酶（MCAD）和非常长链的酰基辅酶A脱氢酶（VCAD）酶活性测定的方法。 该方法可以包括但不限于制备样品; 制备酶特异性底物/试剂混合物; 将制备的样品的等分试样与酶特异性底物/试剂混合物的等分试样混合; 读取吸光度在约600nm范围内; 孵育制备的样品和酶特异性底物/试剂混合物; 并以各种时间间隔读取约600nm范围内的吸光度。

公开(公告)号：US20150212043A1

公开(公告)日：2015-07-30

申请号：US14428219

申请日：2013-10-14

Applicant: ADVANCED LIQUID LOGIC, INC.

Inventor： Michael G. Pollack

IPC: G01N27/447

CPC classification number: G01N27/44791 ,  B01L3/0241 ,  B01L3/502792 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2300/088 ,  B01L2300/0883 ,  B01L2300/1827 ,  B01L2400/0427 ,  B01L2400/043 ,  B01L2400/0457 ,  G01N27/44743

Abstract: A liquid handling system for supplying liquids to a flow cell (FC). The system may include a droplet actuator cartridge, wherein the droplet actuator and a flow cell are fluidly coupled to, or situated within, a droplet operations gap of the droplet actuator.

Abstract translation: 一种用于向流动池（FC）供应液体的液体处理系统。 该系统可以包括液滴致动器盒，其中液滴致动器和流动池流体耦合到液滴致动器的液滴操作间隙或位于液滴致动器的液滴操作间隙内。

公开(公告)号：US09086345B2

公开(公告)日：2015-07-21

申请号：US14308110

申请日：2014-06-18

Applicant: Advanced Liquid Logic, Inc.

Inventor： Ramakrishna Sista ,  Vamsee K. Pamula ,  Vijay Srinivasan ,  Michael G. Pollack ,  Allen Eckhardt

IPC: G01N15/06 ,  G01N33/00 ,  G01N33/48 ,  G01N1/38 ,  B01F11/00 ,  B01F13/00 ,  B01L3/00 ,  B01L7/00 ,  G01N35/10

CPC classification number: G01N33/54326 ,  B01F11/0071 ,  B01F13/0071 ,  B01F13/0076 ,  B01F13/0809 ,  B01L3/50273 ,  B01L3/502761 ,  B01L3/502792 ,  B01L7/52 ,  B01L7/525 ,  B01L2200/027 ,  B01L2200/0647 ,  B01L2200/0673 ,  B01L2300/06 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/089 ,  B01L2300/1827 ,  B01L2400/0406 ,  B01L2400/0415 ,  B01L2400/0421 ,  B01L2400/0424 ,  B01L2400/0427 ,  B01L2400/043 ,  B01L2400/0436 ,  B01L2400/0442 ,  G01N1/38 ,  G01N2001/386 ,  G01N2035/1046 ,  Y10T436/11 ,  Y10T436/25 ,  Y10T436/25625 ,  Y10T436/2575

Abstract: The invention provides a method of dispersing or circulating magnetically responsive beads within a droplet in a droplet actuator. The invention, in one embodiment, makes use of a droplet actuator with a plurality of droplet operations electrodes configured to transport the droplet, and a magnetic field present at a portion of the plurality of droplet operations electrodes. A bead-containing droplet is provided on the droplet actuator in the presence of the uniform magnetic field. Beads are circulated in the droplet during incubation by conducting droplet operations on the droplet within a uniform region of the magnate field. Other embodiments are also provided.

Abstract translation: 本发明提供了一种在液滴致动器中的液滴内分散或循环磁响应珠的方法。 在一个实施例中，本发明利用具有配置成传送液滴的多个液滴操作电极的液滴致动器和存在于多个液滴操作电极的一部分处的磁场。 在均匀磁场存在的情况下，在液滴致动器上提供含珠的液滴。 在孵育期间，通过在大气场的均匀区域内的液滴上进行液滴操作，珠子在液滴中循环。 还提供了其他实施例。

公开(公告)号：US09012165B2

公开(公告)日：2015-04-21

申请号：US14089070

申请日：2013-11-25

Applicant: Advanced Liquid Logic, Inc.

Inventor： Allen E. Eckhardt

IPC: C12Q1/54 ,  C07D311/18 ,  C07D311/20 ,  C07H15/203 ,  C12Q1/25 ,  C12Q1/34 ,  G01N21/64 ,  G01N33/543 ,  B01L3/00

CPC classification number: C12Q1/40 ,  B01L3/502784 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/18 ,  B01L2400/0427 ,  B01L2400/043 ,  C07D311/16 ,  C07D311/18 ,  C07D311/20 ,  C07D405/12 ,  C07D413/12 ,  C07H15/203 ,  C07H17/075 ,  C12Q1/25 ,  C12Q1/34 ,  C12Q1/44 ,  C12Q1/54 ,  C12Y302/01023 ,  G01N21/6452 ,  G01N33/5438 ,  G01N2333/916 ,  G01N2333/926 ,  G01N2333/938 ,  G01N2800/04

Abstract: The invention is directed to an assay for acid β-galactosidase activity. The invention may include combining in oil a sample droplet with a 4-methylumbelliferyl-B-galactose to yield a reaction droplet; splitting the reaction droplet to yield a first daughter droplet and a second daughter droplet; combining the first daughter droplet with a stop buffer droplet to yield a first stopped reaction droplet; incubating the second daughter droplet; combining the second daughter droplet with a stop buffer droplet to yield a second stopped reaction droplet; and measuring 4-methylumbelliferone released in the first and second stopped reaction droplets.

Abstract translation: 本发明涉及酸和半乳糖苷酶活性的测定。 本发明可以包括在油中混合样品液滴与4-甲基伞形酮基-B-半乳糖以产生反应液滴; 分裂反应液滴以产生第一子液滴和第二子液滴; 将第一子液滴与停止缓冲液滴组合以产生第一停止的反应液滴; 孵育第二个子液滴; 将第二子液滴与停止缓冲液滴组合以产生第二停止的反应液滴; 并测量在第一和第二停止反应液滴中释放的4-甲基伞形酮。

公开(公告)号：US20150065360A1

公开(公告)日：2015-03-05

申请号：US14538379

申请日：2014-11-11

Applicant: Advanced Liquid Logic, Inc.

Inventor： Allen E. Eckhardt ,  Jonathan Benton ,  Deborah Boles

IPC: C12N15/10 ,  C12Q1/70 ,  C12Q1/68

CPC classification number: C12N15/1089 ,  C12Q1/6869 ,  C12Q1/689 ,  C12Q1/701 ,  C12Q1/703 ,  G16B30/00 ,  G16B40/00 ,  C12Q2537/165 ,  C12Q2565/301

Abstract: The systems and methods of the invention provide a guided approach to pyrosequencing (i.e., hybrid pyrosequencing). A de novo nucleic acid sequence may compared to a library of possible results and the next nucleotide to be dispensed is selected based on the comparison of the de novo sequence and the library of possible results. In another example, at least the first nucleotide to be dispensed is selected based on a query of a database(s) of non-sequence parameters (e.g., incidence of infection, diagnostic symptoms, sample source) and subsequent dispensations determined based on a comparison of the de novo sequence and the library of possible results (e.g., candidate sequences). The systems and methods of the invention may be performed using a droplet actuator.

Abstract translation: 本发明的系统和方法提供了焦磷酸测序（即，杂化焦磷酸测序）的指导方法。 从头核酸序列可以与可能结果的文库进行比较，并且基于从头序列与可能结果库的比较来选择待分配的下一个核苷酸。 在另一个实例中，基于对非序列参数（例如，感染发生率，诊断症状，样本来源）的数据库的查询和基于比较确定的随后分配来选择至少要分配的第一核苷酸 的新生序列和可能结果的库（例如候选序列）。 本发明的系统和方法可以使用液滴致动器来执行。