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    POLYMERASE COMPOSITIONS AND KITS, AND METHODS OF USING AND MAKING THE SAME
    42.
    发明公开
    POLYMERASE COMPOSITIONS AND KITS, AND METHODS OF USING AND MAKING THE SAME 审中-公开

    公开(公告)号：US20240101980A1

    公开(公告)日：2024-03-28

    申请号：US18521466

    申请日：2023-11-28

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Daniel MAZUR ,  Sihong CHEN ,  Peter VANDER HORN ,  Eileen TOZER ,  Guobin LUO ,  Joshua SHIRLEY ,  Kevin HEINEMANN

    IPC: C12N9/12 ,  C12Q1/686

    CPC classification number: C12N9/1252 ,  C12Q1/686 ,  C12Y207/07007

    Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, recombinant polymerases and biologically active fragments thereof are provided that allow for nucleic acid amplification. In some aspects, the disclosure provides recombinant polymerases that yield lower systematic error rates and/or improved accuracy, when used in sequencing by synthesis reactions as compared to a control polymerase. In one aspect, the disclosure relates to recombinant polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In another aspect, the recombinant polymerases are useful for the amplification of nucleic acid templates during PCR, emPCR, isothermal amplification, recombinase polymerase amplification, rolling circle amplification, strand displacement amplification and proximity ligation amplification. In some aspects, the disclosure relates to recombinant polymerases useful for the generation of nucleic acid libraries and/or nucleic acid templates.

    Systems And Methods For Improved Imaging And Fluorescence In Flow Cytometry And Other Applications
    43.
    发明公开
    Systems And Methods For Improved Imaging And Fluorescence In Flow Cytometry And Other Applications 审中-公开

    公开(公告)号：US20240094110A1

    公开(公告)日：2024-03-21

    申请号：US18240441

    申请日：2023-08-31

    Applicant: Life Technologies Corporation

    Inventor： Michael D Ward ,  Gregory Kaduchak ,  Dmitriy Strelkov

    IPC: G01N15/14

    CPC classification number: G01N15/1436 ,  G01N2015/0065

    Abstract: Provided are systems and methods that allow for brightfield imaging in a flow cytometer, allowing for collection of fluorescence and high-quality image date. The disclosed technology also gives rise to an illumination pattern that allows a user to create different oblique or structured illumination profiles within a static system. With the disclosed approach, a user can illuminate a sample from a first direction (e.g., with laser illumination configured to give rise to one or more of fluorescence information and scattering information), collect scattering information from a second direction, collect fluorescence information from a third direction, and capture an image of the sample from a fourth direction. (Two or more of the foregoing can be accomplished simultaneously.) Also as described elsewhere herein, an illumination used to illuminate the sample for visual image capture can be communicated to the same through a lens that also collects fluorescence from the sample.

    NUCLEIC ACID AMPLIFICATION
    44.
    发明公开
    NUCLEIC ACID AMPLIFICATION 审中-公开

    公开(公告)号：US20240067939A1

    公开(公告)日：2024-02-29

    申请号：US18221099

    申请日：2023-07-12

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Chieh-Yuan LI ,  David RUFF ,  Shiaw-Min CHEN ,  Jennifer O'NEIL ,  Rachel KASINSKAS ,  Jonathan ROTHBERG ,  Bin LI ,  Kai Qin LAO

    IPC: C12N9/12 ,  C12Q1/6844 ,  C12Q1/6853 ,  C12Q1/6855 ,  C12Q1/686

    CPC classification number: C12N9/1252 ,  C12Q1/6846 ,  C12Q1/6853 ,  C12Q1/6855 ,  C12Q1/686 ,  C12Q1/6874

    Abstract: The present disclosure provides methods, compositions, kits and systems for nucleic acid amplification. In some embodiments, nucleic acid amplification methods include subjecting the nucleic acid to be amplified to partially denaturing conditions. In some embodiments, nucleic acid amplification methods include amplifying without fully denaturing the nucleic acid that is amplified. In some embodiments, the nucleic acid amplification method employs an enzyme that catalyzes homologous recombination and a polymerase. In some embodiments, methods for nucleic acid amplification can be conducted in a single reaction vessel and/or in a single continuous liquid phase of a reaction mixture, without need for compartmentalization of the reaction mixture or immobilization of reaction components. In some embodiments, methods for nucleic acid amplification comprise amplifying at least one polynucleotide onto a surface under isothermal amplification conditions, optionally in the presence of a polymer which can include a sieving agent and/or a diffusion-reducing agent.

    Microcarrier filter bag assemblies and methods of use
    45.
    发明授权
    Microcarrier filter bag assemblies and methods of use 有权

    公开(公告)号：US11890557B2

    公开(公告)日：2024-02-06

    申请号：US17740710

    申请日：2022-05-10

    Applicant: Life Technologies Corporation

    Inventor： Charles R. Rhodes

    IPC: B01D29/27 ,  C12M1/12 ,  C12M1/00 ,  C12M1/26 ,  B01D35/027

    CPC classification number: B01D29/27 ,  C12M23/14 ,  C12M23/28 ,  C12M25/16 ,  C12M29/04 ,  C12M33/14 ,  C12M47/02 ,  B01D35/027

    Abstract: A filter bag assembly includes: a flexible first sheet; a flexible second sheet overlying and secured to the first sheet so that a compartment is formed therebetween; a porous filter sheet disposed between the first sheet and the second sheet so as to divide the compartment into a first compartment and a second compartment; a first port secured to the second sheet so as to communicate directly with the first compartment; and a second port secured to the second sheet so as to communicate directly with the second compartment, the second port being spaced apart from the first port. The porous filter sheet is disposed so that fluid entering the first compartment through the first port must pass through the filter sheet before entering the second compartment or exiting through the second port.

    Polymerase compositions and kits, and methods of using and making the same
    48.
    发明授权
    Polymerase compositions and kits, and methods of using and making the same 有权

    公开(公告)号：US11866740B2

    公开(公告)日：2024-01-09

    申请号：US17302621

    申请日：2021-05-07

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Daniel Mazur ,  Peter B. Vander Horn ,  Eileen Tozer ,  Sihong Chen ,  Guobin Luo ,  Joshua Shirley ,  Kevin Heinemann

    IPC: C12N9/12 ,  C12Q1/686

    CPC classification number: C12N9/1252 ,  C12Q1/686 ,  C12Y207/07007 ,  C12Q1/686 ,  C12Q2521/101

    Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, recombinant polymerases and biologically active fragments thereof are provided that allow for nucleic acid amplification. In some aspects, the disclosure provides recombinant polymerases that yield lower systematic error rates and/or improved accuracy, when used in sequencing by synthesis reactions as compared to a control polymerase. In one aspect, the disclosure relates to recombinant polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In another aspect, the recombinant polymerases are useful for the amplification of nucleic acid templates during PCR, emPCR, isothermal amplification, recombinase polymerase amplification, rolling circle amplification, strand displacement amplification and proximity ligation amplification. In some aspects, the disclosure relates to recombinant polymerases useful for the generation of nucleic acid libraries and/or nucleic acid templates.

    Optical systems and methods for sample separation
    50.
    发明授权
    Optical systems and methods for sample separation 有权

    公开(公告)号：US11860122B2

    公开(公告)日：2024-01-02

    申请号：US18098060

    申请日：2023-01-17

    Applicant: Life Technologies Corporation

    Inventor： Shaohong Wang

    IPC: G01J3/44 ,  G01N27/447 ,  G01J3/02 ,  G01J3/18

    CPC classification number: G01N27/44721 ,  G01J3/0208 ,  G01J3/0218 ,  G01J3/0289 ,  G01J3/0294 ,  G01J3/18 ,  G01J3/4406 ,  G01N27/44791

    Abstract: A system for separating biological molecules includes a plurality of capillaries, a capillary mount, a plurality of optical fibers, a fiber mount, an optical detector, and a motion stage. The plurality of capillaries are configured to separate biological molecules in a sample. Each capillary comprising a detection portion configured to pass electromagnetic radiation into the capillary. The plurality of capillaries are coupled to the capillary mount such that the detection portions are fixedly located relative to one another. Each optical fiber includes a receiving end to receive emissions. The optical fibers are coupled to the fiber mount such that the receiving ends of the optical fibers are fixedly located relative to one another. The optical detector is configured to produce an alignment signal. The motion stage is configured to align the receiving ends of the optical fibers to the detection portions based on values of the alignment signal.

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