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    DETERMINATION OF NUCLEIC ACID METHYLATION
    41.
    发明申请
    DETERMINATION OF NUCLEIC ACID METHYLATION 审中-公开

    公开(公告)号：US20190017110A1

    公开(公告)日：2019-01-17

    申请号：US15778876

    申请日：2016-11-25

    Applicant: Agency for Science, Technology and Research

    Inventor： Yanbing Zu ,  Jackie Y. Ying

    IPC: C12Q1/6858 ,  C12Q1/6886

    Abstract: The invention relates to methods and kits for determining the methylation status of a target nucleic acid molecule in a sample comprising (a) bisulfite treatment of the target nucleic acid, (b) amplifying the treated target nucleic acid with methylation-specific primers, (c) contacting the amplicons with a methylation-specific plasmonic nanoprobe and (d) determining the methylation status based on melting temperature Tm of the hybrid probe and the target nucleic acid. In particular, a plasmonic gold nanoparticle covalently coupled to a morpholino oligonucleotide probe. Also claimed are methods and kits for determining methylation status of the Septin 9 (SEPT9) gene promoter.

    NANOSTRUCTURED CONTACT LENSES AND RELATED OPHTHALMIC MATERIALS
    46.
    发明申请
    NANOSTRUCTURED CONTACT LENSES AND RELATED OPHTHALMIC MATERIALS 审中-公开
    NANOSTRUCTURED接触镜和相关眼镜材料

    公开(公告)号：US20150168605A1

    公开(公告)日：2015-06-18

    申请号：US14569253

    申请日：2014-12-12

    Applicant: Agency for Science, Technology and Research

    Inventor： Jackie Y. Ying ,  Shona Pek

    IPC: G02B1/04 ,  G02C7/10

    CPC classification number: G02B1/043 ,  C08L33/10

    Abstract: A bicontinuous microemulsion of water, a monomer, and a surfactant copolymerizable with the monomer is polymerized to form a polymeric material, the polymeric material comprising a polymer matrix defining interconnected pores. The polymeric material may additional comprise at least one photochromic agent and/or UV-absorbing agent. The at least one photochromic agent and/or UV-absorbing agent may be dispersed in one or both of the polymer matrix or the interconnected pores. The polymeric material may be used to form photochromic articles including ophthalmic articles such as contact lenses.

    Abstract translation: 水，单体和可与单体共聚的表面活性剂的双连续微乳液被聚合以形成聚合材料，聚合物材料包含限定互连孔的聚合物基质。 聚合物材料可另外包含至少一种光致变色剂和/或紫外线吸收剂。 所述至少一种光致变色剂和/或UV吸收剂可以分散在聚合物基质或互连孔中的一个或两个中。 聚合物材料可用于形成光致变色制品，包括眼用制品如隐形眼镜。

    A METHOD TO AMPLIFY A NUCLEIC ACID
    48.
    发明申请
    A METHOD TO AMPLIFY A NUCLEIC ACID 有权

    公开(公告)号：US20230086471A1

    公开(公告)日：2023-03-23

    申请号：US17799882

    申请日：2021-02-15

    Applicant: Agency for Science, Technology and Research

    Inventor： Jackie Y. Ying ,  Muhammad Nadjad Bin Abdul Rahim

    IPC: C12Q1/6844

    Abstract: This invention relates to methods and compositions for amplifying nucleic acids, e.g., genomic DNA, using nicking agents. The method of amplifying nucleic acids comprising: (a) forming a reaction mixture comprising: (i) a first nucleic acid template comprising a strand having a first nicking agent recognition sequence; (ii) a second nucleic acid template comprising a strand having a second nicking agent recognition sequence; (iii) at least one primer for a target region on the first or second nucleic acid template; (iv) at least one protein having DNA polymerase domain function, wherein the domain function comprises a first domain function capable of strand displacement activity and a second domain function capable of high processivity activity, or at least one protein having DNA polymerase domain function capable of strand displacement activity and at least one protein having DNA polymerase domain function capable of high processivity activity; (v) at least one deoxynucleoside triphosphate; and (vi) a first nicking agent for recognizing the first nicking agent recognition sequence and a second nicking agent for recognizing the second nicking agent recognition sequence; (b) incubating the reaction mixture under conditions that amplifies the nucleic acid templates, wherein the domain functions capable of strand displacement activity and high processivity activity are separate from each other and capable of carrying out their activities simultaneously. In specific embodiments, the nicking agent is NB.BsrDI and the proteins having DNA polymerase domain functions are Bst 3.0 polymerase and Pfu polymerase.

    IMMUNE RESPONSE PROFILING OF TUMOR-DERIVED EXOSOMES FOR CANCER DIAGNOSIS
    49.
    发明申请
    IMMUNE RESPONSE PROFILING OF TUMOR-DERIVED EXOSOMES FOR CANCER DIAGNOSIS 有权

    公开(公告)号：US20210223251A1

    公开(公告)日：2021-07-22

    申请号：US16761827

    申请日：2018-12-06

    Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH

    Inventor： Jackie Y. Ying ,  Yiran Zheng ,  Yi Fei Lee

    IPC: G01N33/574

    Abstract: Disclosed herein are methods of (i) detecting cancer or cancer type in a subject or (ii) simultaneously testing for, or distinguishing between, multiple types of cancer in a subject; methods of screening subjects for a prevalence of cancer type or cancer types; methods of managing a subject with a cancer type; and methods of identifying whether a subject having a cancer type is responding to management of that cancer type; and methods of generating a response profile specific for a cancer type. Disclosed herein also include tumor-derived exosome-induced immune response or cancer-specific response profile created based on the measurement of functional impacts of tumor-derived exosomes on immune cells in vitro for use or when used for detecting or diagnosing cancer or cancer type in a subject; tumor-derived exosome-induced immune response for use in or when used for creating a cancer-specific response profile measuring functional impacts of tumor-derived exosomes on immune cells in vitro; and use of a tumor-derived exosome-induced immune response for generating a cancer-specific response profile measuring functional impacts of tumor-derived exosomes on immune cells in vitro. Disclosed herein also include tests, assays, kits, apparatus or devises for use or when used for the method, the response, the profile or the use as disclosed herein.

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