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公开(公告)号：US20240344005A1
公开(公告)日：2024-10-17
申请号：US18631480
申请日：2024-04-10
Applicant: EMULATE, Inc.
Inventor： Geraldine A. Hamilton , Norman Wen , Catherine Karalis , Antonio Varone , Daniel Levner , Riccardo Barrile
CPC classification number: C12M23/16 , C12M25/02 , C12N5/0693 , G01N33/5011 , G01N33/5017
Abstract: The invention generally relates to a microfluidic platforms or “chips” for testing and understanding cancer, and, more specifically, for understanding the factors that contribute to cancer invading tissues and causing metastases. Tumor cells are grown on microfluidic devices with other non-cancerous tissues under conditions that simulate tumor invasion. The interaction with immune cells can be tested to inhibit this activity by linking a cancer chip to a lymph chip.
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公开(公告)号：US12098352B2
公开(公告)日：2024-09-24
申请号：US16983850
申请日：2020-08-03
Applicant: EMULATE, INC.
Inventor： Janna Nawroth , Riccardo Barrile , David Conegliano , Remi Villenave , Carolina Lucchesi , Justin Nguyen , Antonio Varone , Catherine Karalis , Geraldine Hamilton
CPC classification number: C12M23/16 , B01L3/5027 , C12N5/0688 , C12N5/0696 , C12N2501/115 , C12N2501/117 , C12N2501/119 , C12N2501/155 , C12N2501/41 , C12N2502/1323 , C12N2502/27 , C12N2503/04 , C12N2506/02 , C12N2513/00 , G01N2800/12
Abstract: An in vitro microfluidic “organ-on-chip” device is described herein that mimics the structure and at least one function of specific areas of the epithelial system in vivo. In particular, a stem cell-based Lung-on-Chip is described. This in vitro microfluidic system can be used for modeling differentiation of cells on-chip into lung cells, e.g., a lung (Lung-On-Chip), bronchial (Airway-On-Chip; small-Airway-On-Chip), alveolar sac (Alveolar-On-Chip), etc., for use in modeling disease states of derived tissue, i.e. as healthy, pre-disease and diseased tissues. Additionally, stem cells under differentiation protocols for deriving (producing) differentiated lung cells off-chips may be seeded onto microfluidic devices at any desired point during the in vitro differentiation pathway for further differentiation on-chip or placed on-chip before, during or after terminal differentiation.
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公开(公告)号：US20240301358A1
公开(公告)日：2024-09-12
申请号：US18602727
申请日：2024-03-12
Applicant: EMULATE, Inc.
Inventor： Athanasia Apostolou , Antonio Varone , Magdalena Kasendra , Raymond Luc
CPC classification number: C12N5/0679 , B01L3/502715 , B01L3/502761 , C12M23/16 , C12M23/26 , C12M25/02 , C12N5/069 , G01N1/30 , G01N33/5047 , G01N33/5064 , B01L2200/16 , B01L2300/123 , B01L2300/16 , C12N2500/00 , C12N2501/052 , C12N2501/2301 , C12N2501/2306 , C12N2501/25
Abstract: An in vitro microfluidic intestine on-chip is described herein that mimics the structure and at least one function of specific areas of the gastrointestinal system in vivo. In particular, a multicellular, layered, microfluidic intestinal cell culture, which is some embodiments is derived from patient's enteroids-derived cells, is described comprising L cells, allowing for interactions between L cells and gastrointestinal epithelial cells, endothelial cells and immune cells. This in vitro microfluidic system can be used for modeling inflammatory gastrointestinal autoimmune tissue, e.g., diabetes, obesity, intestinal insufficiency and other inflammatory gastrointestinal disorders. These multicellular-layered microfluidic intestine on-chips further allow for comparisons between types of gastrointestinal tissues, e.g., small intestinal duodenum, small intestinal jejunum, small intestinal ileum, large intestinal colon, etc., and between disease states of gastrointestinal tissue, i.e. healthy, pre-disease and diseased areas. Additionally, these microfluidic gut-on-chips allow identification of cells and cellular derived factors driving disease states and drug testing for reducing inflammation.
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公开(公告)号：US12018284B2
公开(公告)日：2024-06-25
申请号：US17984023
申请日：2022-11-09
Applicant: EMULATE, INC.
Inventor： Athanasia Apostolou , Antonio Varone , Magdalena Kasendra , Raymond Luc
CPC classification number: C12N5/0679 , B01L3/502715 , B01L3/502761 , C12M23/16 , C12M23/26 , C12M25/02 , C12N5/069 , G01N1/30 , G01N33/5047 , G01N33/5064 , B01L2200/16 , B01L2300/123 , B01L2300/16 , C12N2500/00 , C12N2501/052 , C12N2501/2301 , C12N2501/2306 , C12N2501/25
Abstract: An in vitro microfluidic intestine on-chip is described herein that mimics the structure and at least one function of specific areas of the gastrointestinal system in vivo. In particular, a multicellular, layered, microfluidic intestinal cell culture, which is some embodiments is derived from patient's enteroids-derived cells, is described comprising L cells, allowing for interactions between L cells and gastrointestinal epithelial cells, endothelial cells and immune cells. This in vitro microfluidic system can be used for modeling inflammatory gastrointestinal autoimmune tissue, e.g., diabetes, obesity, intestinal insufficiency and other inflammatory gastrointestinal disorders. These multicellular-layered microfluidic intestine on-chips further allow for comparisons between types of gastrointestinal tissues, e.g., small intestinal duodenum, small intestinal jejunum, small intestinal ileum, large intestinal colon, etc., and between disease states of gastrointestinal tissue, i.e. healthy, pre-disease and diseased areas. Additionally, these microfluidic gut-on-chips allow identification of cells and cellular derived factors driving disease states and drug testing for reducing inflammation.
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公开(公告)号：US11970680B2
公开(公告)日：2024-04-30
申请号：US16089220
申请日：2017-03-30
Applicant: EMULATE, INC.
Inventor： Geraldine A. Hamilton , Norman Wen , Catherine Karalis , Antonio Varone , Daniel Levner , Riccardo Barrile
CPC classification number: C12M23/16 , C12M25/02 , C12N5/0693 , G01N33/5011 , G01N33/5017
Abstract: The invention generally relates to a microfluidic platforms or “chips” for testing and understanding cancer, and, more specifically, for understanding the factors that contribute to cancer invading tissues and causing metastases. Tumor cells are grown on microfluidic devices with other non-cancerous tissues under conditions that simulate tumor invasion. The interaction with immune cells can be tested to inhibit this activity by linking a cancer chip to a lymph chip.
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Patent Agency Ranking
公开(公告)号：US11859165B2
公开(公告)日：2024-01-02
申请号：US18097755
申请日：2023-01-17
Applicant: EMULATE, INC.
Inventor： Daniel Levner , Christopher David Hinojosa , Norman Wen , Antonio Varone , Justin Nguyen , Lina Williamson , S. Jordan Kerns , Catherine Karalis , Geraldine Hamilton , Carol Lucchesi
IPC: C12M3/06 , C12M1/00 , C12M1/12 , C12M1/42 , G01N33/50 , G01N33/543 , C12N5/0793 , C12N5/079 , C12N5/071 , C12N5/077
CPC classification number: C12M35/04 , C12M23/16 , C12M23/38 , C12M25/02 , C12M35/08 , C12N5/069 , C12N5/0619 , C12N5/0622 , C12N5/0629 , C12N5/0656 , C12N5/0688 , C12N5/0697 , C12N5/0698 , G01N33/50 , G01N33/5082 , G01N33/54366 , C12N2501/165
Abstract: A microfluidic device is contemplated comprising an open-top cavity with structural anchors on the vertical wall surfaces that serve to prevent gel shrinkage-induced delamination, a porous membrane (optionally stretchable) positioned in the middle over a microfluidic channel(s). The device is particularly suited to the growth of cells mimicking dermal layers.
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公开(公告)号：US20230341378A1
公开(公告)日：2023-10-26
申请号：US18214651
申请日：2023-06-27
Applicant: EMULATE, Inc.
Inventor： Daniel Levner , Kyung Jin Jang , Jacob Fraser , S. Jordan Kerns , Antonio Varone , Dongeun Huh
IPC: G01N33/50 , C12N5/071 , C12M1/12 , C12N5/077 , C12N5/00 , C07K2/00 , C07K14/78 , C12M3/06 , C12M1/00
CPC classification number: G01N33/5032 , C07K2/00 , C07K14/78 , C12M23/16 , C12M23/20 , C12M25/02 , C12N5/0018 , C12N5/0068 , C12N5/0658 , C12N5/067 , G01N33/5014 , C12N2500/32 , C12N2521/00 , C12N2533/30 , C12N2533/50 , C12N2533/52 , C12N2533/54 , C12N2533/90 , C12N2535/10 , C12N2537/10 , G01N33/5044
Abstract: Compositions, devices and methods are described for improving adhesion, attachment, and/or differentiation of cells in a microfluidic device or chip. In one embodiment, one or more ECM proteins are covalently coupled to the surface of a microchannel of a microfluidic device. The microfluidic devices can be stored or used immediately for culture and/or support of living cells such as mammalian cells, and/or for simulating a function of a tissue, e.g., a liver tissue, muscle tissue, etc. Extended adhesion and viability with sustained function over time is observed.
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公开(公告)号：US20230287324A1
公开(公告)日：2023-09-14
申请号：US18097755
申请日：2023-01-17
Applicant: EMULATE, INC.
Inventor： Daniel Levner , Christopher David Hinojosa , Norman Wen , Antonio Varone , Justin Nguyen , Lina Williamson , S. Jordan Kerns , Catherine Karalis , Geraldine Hamilton , Carol Lucchesi
IPC: C12M1/42 , C12M3/06 , C12M1/12 , G01N33/50 , G01N33/543 , C12M1/00 , C12N5/0793 , C12N5/079 , C12N5/071 , C12N5/077
CPC classification number: C12M35/04 , C12M23/16 , C12M23/38 , C12M25/02 , C12M35/08 , C12N5/0619 , C12N5/0622 , C12N5/0629 , C12N5/0656 , C12N5/0688 , C12N5/069 , C12N5/0697 , C12N5/0698 , G01N33/50 , G01N33/5082 , G01N33/54366 , C12N2501/165
Abstract: A microfluidic device is contemplated comprising an open-top cavity with structural anchors on the vertical wall surfaces that serve to prevent gel shrinkage-induced delamination, a porous membrane (optionally stretchable) positioned in the middle over a microfluidic channel(s). The device is particularly suited to the growth of cells mimicking dermal layers.
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公开(公告)号：US20230151333A1
公开(公告)日：2023-05-18
申请号：US17984023
申请日：2022-11-09
Applicant: EMULATE, INC.
Inventor： Athanasia Apostolou , Antonio Varone , Magdalena Kasendra , Raymond Luc
CPC classification number: C12N5/0679 , C12M23/16 , C12M25/02 , G01N1/30
Abstract: An in vitro microfluidic intestine on-chip is described herein that mimics the structure and at least one function of specific areas of the gastrointestinal system in vivo. In particular, a multicellular, layered, microfluidic intestinal cell culture, which is some embodiments is derived from patient's enteroids-derived cells, is described comprising L cells, allowing for interactions between L cells and gastrointestinal epithelial cells, endothelial cells and immune cells. This in vitro microfluidic system can be used for modeling inflammatory gastrointestinal autoimmune tissue, e.g., diabetes, obesity, intestinal insufficiency and other inflammatory gastrointestinal disorders. These multicellular-layered microfluidic intestine on-chips further allow for comparisons between types of gastrointestinal tissues, e.g., small intestinal duodenum, small intestinal jejunum, small intestinal ileum, large intestinal colon, etc., and between disease states of gastrointestinal tissue, i.e. healthy, pre-disease and diseased areas. Additionally, these microfluidic gut-on-chips allow identification of cells and cellular derived factors driving disease states and drug testing for reducing inflammation.
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公开(公告)号：US11549937B2
公开(公告)日：2023-01-10
申请号：US15648306
申请日：2017-07-12
Applicant: Emulate Inc.
Inventor： Daniel Levner , Kyung Jin Jang , Jacob Fraser , S. Jordan Kerns , Antonio Varone , Dongeun Huh
IPC: C12N5/00 , C12N5/02 , A61K38/00 , C07K2/00 , C07K4/00 , C07K5/00 , C07K7/00 , C07K14/00 , C07K16/00 , C07K17/00 , G01N33/50 , C07K14/78 , C12N5/071 , C12M3/06 , C12M1/00 , C12M1/12 , C12N5/077
Abstract: Compositions, devices and methods are described for improving adhesion, attachment, and/or differentiation of cells in a microfluidic device or chip. In one embodiment, one or more ECM proteins are covalently coupled to the surface of a microchannel of a microfluidic device. The microfluidic devices can be stored or used immediately for culture and/or support of living cells such as mammalian cells, and/or for simulating a function of a tissue, e.g., a liver tissue, muscle tissue, etc. Extended adhesion and viability with sustained function over time is observed.
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