公开(公告)号：US20180353958A1

公开(公告)日：2018-12-13

申请号：US15781235

申请日：2016-12-02

Applicant: President and Fellows of Harvard College

Inventor： Christopher David Hinojosa ,  Daniel Levner

IPC: B01L3/00 ,  C09K3/00 ,  C08F212/08 ,  B01J4/00 ,  B01L9/00

CPC classification number: B01L3/502715 ,  B01J4/00 ,  B01J4/001 ,  B01L3/565 ,  B01L9/54 ,  B01L2200/027 ,  B01L2200/0689 ,  B01L2300/0816 ,  B01L2300/0838 ,  C08F212/08 ,  C09K3/00 ,  G01N1/28

Abstract: The present invention relates to microfluidic systems, devices and methods. More specifically, the invention relates to gaskets for sealing fluid interfaces in microfluidic systems and devices.

公开(公告)号：US20180346859A1

公开(公告)日：2018-12-06

申请号：US15781078

申请日：2016-12-02

Applicant: President and Fellows of Harvard College

Inventor： Antonio Varone ,  Norman Wen ,  Daniel Levner ,  Richard Novak ,  Lori McPartlin ,  Donald E. Ingber ,  Youngjae Choe ,  Lian Leng ,  Justin K. Nguyen

IPC: C12M3/06 ,  C12M3/00 ,  C12M1/12 ,  C12N5/071 ,  C12N5/077

CPC classification number: C12M25/02 ,  C12M21/08 ,  C12M23/16

Abstract: A device for simulating a function of a tissue includes a first structure, a second structure, and a membrane. The first structure defines a first chamber. The first chamber includes a matrix disposed therein and an opened region. The second structure defines a second chamber. The membrane is located at an interface region between the first chamber and the second chamber. The membrane includes a first side facing toward the first chamber and a second side facing toward the second chamber. The membrane separates the first chamber from the second chamber.

公开(公告)号：US20180071690A1

公开(公告)日：2018-03-15

申请号：US15558191

申请日：2016-03-16

Applicant: President and Fellows of Harvard College

Inventor： James Coon ,  Tiama Hamkins-Indik ,  Donald E. Ingber ,  Miles Ingram ,  Daniel Levner ,  Richard Novak ,  Jefferson Puerta ,  Daniel E. Shea ,  Josiah Sliz ,  Norman Wen

IPC: B01D67/00 ,  B01D71/70 ,  B29C41/38 ,  B29C33/42 ,  B29C33/68 ,  B29C39/00 ,  B29C39/26 ,  B29C41/12 ,  C12M1/12

CPC classification number: B01D67/0034 ,  B01D71/70 ,  B01L3/502707 ,  B01L2300/123 ,  B01L2400/086 ,  B29C33/424 ,  B29C33/68 ,  B29C39/006 ,  B29C39/26 ,  B29C41/12 ,  B29C41/36 ,  B29C41/38 ,  B29C2043/025 ,  B29L2031/755 ,  B29L2031/756 ,  C12M25/02

Abstract: A method for micro-molding a polymeric membrane and including pouring a predetermined volume of curable polymer unto a micro-fabricated mold having a post array with pillars, and overlaying the polymer with a support substrate. A spacer, such as a rubber spacer, is placed in contact with the support substrate and a force is applied to an exposed side of the spacer to compress the support substrate and the polymer together. While applying the force, the polymer is cured on the mold for a predetermined time period and at a predetermined temperature to form a polymeric membrane having a pore array with a plurality of pores corresponding to the plurality of pillars of the post array. The polymeric membrane is removed from the support substrate.

公开(公告)号：US20160187636A1

公开(公告)日：2016-06-30

申请号：US14899550

申请日：2014-06-26

Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE

Inventor： Donald E. Ingber ,  Daniel Levner ,  Guy Thompson ,  Christopher D. Hinojosa ,  Jose Fernandez-Alcon

IPC: G02B21/24 ,  G02B21/02 ,  G02B21/16 ,  G02B21/34 ,  G02B21/36 ,  G02B21/14 ,  G02B21/06 ,  G02B7/04

CPC classification number: G02B21/24 ,  G02B7/04 ,  G02B21/02 ,  G02B21/06 ,  G02B21/14 ,  G02B21/16 ,  G02B21/26 ,  G02B21/34 ,  G02B21/36

Abstract: A microscopy system for monitoring of one or more specimens includes a plurality of microscope blades, each microscope blade having at least one objective, at least one illuminator, and at least one detector. The microscopy system also includes a plurality of carriages, each carriage being connected to one or more of the microscope blades, and one or more actuators configured to drive the plurality of carriages along one or more axes, at least some of the plurality of carriages having at least partially overlapping ranges of motion along at least one of the one or more axes. The microscopy system also includes a master controller configured to drive each of the carriages, using the actuator(s), along the one or more axes.

Abstract translation: 用于监测一个或多个标本的显微镜系统包括多个显微镜刀片，每个显微镜刀片具有至少一个物镜，至少一个照明器和至少一个检测器。 显微镜系统还包括多个滑架，每个滑架连接到一个或多个显微镜刀片，以及一个或多个致动器，其配置成沿着一个或多个轴线驱动多个滑架，多个滑架中的至少一些具有 沿所述一个或多个轴中的至少一个轴线的至少部分重叠的运动范围。 显微镜系统还包括主控制器，该主控制器被配置成沿着一个或多个轴使用致动器驱动每个托架。

公开(公告)号：US12065634B2

公开(公告)日：2024-08-20

申请号：US17871054

申请日：2022-07-22

Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE

Inventor： Donald E. Ingber ,  Daniel Levner ,  Guy Thompson, II ,  Jose Fernandez-Alcon ,  Christopher David Hinojosa

IPC: C12M1/00 ,  B01L3/00 ,  B01L3/02 ,  C12M1/12 ,  C12M1/36 ,  C12M3/00 ,  C12M3/06

CPC classification number: C12M29/10 ,  B01L3/502715 ,  C12M21/08 ,  C12M23/16 ,  C12M23/38 ,  C12M23/58 ,  C12M29/00 ,  C12M29/20 ,  C12M37/04 ,  C12M41/48 ,  B01L3/0293 ,  B01L2200/027 ,  B01L2300/0816

Abstract: Systems and methods interconnect cell culture devices and/or fluidic devices by transferring discrete volumes of fluid between devices. A liquid-handling system collects a volume of fluid from at least one source device and deposits the fluid into at least one destination device. In some embodiments, a liquid-handling robot actuates the movement and operation of a fluid collection device in an automated manner to transfer the fluid between the at least one source device and the at least one destination device. In some cases, the at least one source device and the at least one destination device are cell culture devices. The at least one source device and the at least one destination device may be microfluidic or non-microfluidic devices. In some cases, the cell culture devices may be microfluidic cell culture devices. In further cases, the microfluidic cell culture devices may include organ-chips.

公开(公告)号：US20240084235A1

公开(公告)日：2024-03-14

申请号：US18470404

申请日：2023-09-19

Applicant: President and Fellows of Harvard College

Inventor： Jose Fernandez-Alcon ,  Norman Wen ,  Richard Novak ,  Donald E. Ingber ,  Geraldine A. Hamilton ,  Christopher Hinojosa ,  Karel Domansky ,  Daniel Levner ,  Guy Thompson, II ,  Kambez Hajipouran Benam ,  Remi Villenave ,  Thomas Umundum ,  Alfred Paris ,  Georg Bauer

IPC: C12M3/06 ,  B01D61/18 ,  B01D63/08 ,  B01D67/00 ,  B01L3/00 ,  C12M1/00 ,  C12M1/12 ,  C12M1/34 ,  C12M1/42 ,  C12M3/00

CPC classification number: C12M23/16 ,  B01D61/18 ,  B01D63/081 ,  B01D67/0023 ,  B01L3/5027 ,  B01L3/502753 ,  C12M21/08 ,  C12M23/26 ,  C12M25/02 ,  C12M35/04 ,  C12M41/00 ,  C12M41/46 ,  B01L2200/0647 ,  B01L2200/12 ,  B01L2300/0645 ,  B01L2300/0887 ,  B01L2400/0487

Abstract: An organomimetic device includes a microfluidic device that can be used to culture cells in its microfluidic channels. The organomimetic device can be part of dynamic system that can apply mechanical forces to the cells by modulating the microfluidic device and the flow of fluid through the microfluidic channels. The membrane in the organomimetic device can be modulated mechanically via pneumatic means and/or mechanical means. The organomimetic device can be manufactured by the fabrication of individual components separately, for example, as individual layers that can be subsequently laminated together.

公开(公告)号：US20230159872A1

公开(公告)日：2023-05-25

申请号：US18056035

申请日：2022-11-16

Applicant: President and Fellows of Harvard College

Inventor： Antonio Varone ,  Norman Wen ,  Daniel Levner ,  Richard Novak ,  Lori McPartlin ,  Donald E. Ingber ,  Youngjae Choe ,  Lian Leng ,  Justin K. Nguyen

IPC: C12M3/06 ,  C12M3/00 ,  C12M1/12 ,  C12N5/071 ,  C12N5/077

CPC classification number: C12M23/16 ,  C12M21/08 ,  C12M25/02 ,  C12M25/14 ,  C12N5/0629 ,  C12N5/0656 ,  C12N5/0679 ,  C12N5/069 ,  B01L3/5027

Abstract: A device for simulating a function of a tissue includes a first structure, a second structure, and a membrane. The first structure defines a first chamber. The first chamber includes a matrix disposed therein and an opened region. The second structure defines a second chamber. The membrane is located at an interface region between the first chamber and the second chamber. The membrane includes a first side facing toward the first chamber and a second side facing toward the second chamber. The membrane separates the first chamber from the second chamber.

公开(公告)号：US11549136B2

公开(公告)日：2023-01-10

申请号：US17366127

申请日：2021-07-02

Applicant: President and Fellows of Harvard College

Inventor： George M. Church ,  Jehyuk Lee ,  Daniel Levner ,  Michael Super

IPC: C12Q1/6806 ,  C12Q1/6844 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12P19/34 ,  C12Q1/6804 ,  C12Q1/6816 ,  C12Q1/6837 ,  G01N33/53

Abstract: Methods of making a three-dimensional matrix of nucleic acids within a cell is provided.

公开(公告)号：US11499131B2

公开(公告)日：2022-11-15

申请号：US16593646

申请日：2019-10-04

Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE

Inventor： Donald E. Ingber ,  Daniel Levner ,  Guy Thompson, II ,  Jose Fernandez-Alcon ,  Christopher David Hinojosa

IPC: C12M1/00 ,  C12M3/06 ,  B01L3/00 ,  C12M3/00 ,  C12M1/12 ,  C12M1/36 ,  B01L3/02

Abstract: Systems and methods interconnect cell culture devices and/or fluidic devices by transferring discrete volumes of fluid between devices. A liquid-handling system collects a volume of fluid from at least one source device and deposits the fluid into at least one destination device. In some embodiments, a liquid-handling robot actuates the movement and operation of a fluid collection device in an automated manner to transfer the fluid between the at least one source device and the at least one destination device. In some cases, the at least one source device and the at least one destination device are cell culture devices. The at least one source device and the at least one destination device may be microfluidic or non-microfluidic devices. In some cases, the cell culture devices may be microfluidic cell culture devices. In further cases, the microfluidic cell culture devices may include organ-chips.

公开(公告)号：US11311009B2

公开(公告)日：2022-04-26

申请号：US15558103

申请日：2016-03-17

Applicant: President and Fellows of Harvard College

Inventor： Jacob Freake ,  Josh Gomes ,  Christopher David Hinojosa ,  Daniel Levner ,  Doug Sabin ,  Guy Thompson, II

IPC: B01L1/00 ,  A01N1/02 ,  C12M1/00 ,  C12N5/00

Abstract: An incubator assembly includes an incubator enclosure having an internal chamber in which a controlled environment is maintained and which is defined by one or more walls. The incubator assembly further includes a jacket assembly mounted adjacent to at least one of the walls and having an internal airspace in which an internal fluid is enclosed for maintaining a homogenous temperature within the internal chamber. The jacket assembly further has a vent movable between a plurality of positions including an open position in which the internal fluid is allowed to exit the internal airspace into an ambient environment.