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63 条记录 (耗时 0.058 秒)

    Extended dynamic range assays
    42.
    发明授权
    Extended dynamic range assays 失效
    扩展动态范围测定

    公开(公告)号：US06180340B2

    公开(公告)日：2001-01-30

    申请号：US08962033

    申请日：1997-10-31

    申请人： Norman C. Nelson

    发明人： Norman C. Nelson

    IPC分类号： G01N33533

    CPC分类号： G01N33/54306 ,  C12Q1/6816 ,  Y10S435/962 ,  Y10S435/968 ,  Y10S436/80 ,  Y10S436/814 ,  Y10S436/815 ,  Y10S436/817

    摘要： A method and compositions for the detection and/or quantification of an analyte through the use of a plurality of labeled probes, with two or more said probes targeted to different regions of said analyte. In specific embodiments, the labels are separately distinguishable, and/or are present at different specific activities on the labels.

    摘要翻译： 一种用于通过使用多个标记探针来检测和/或定量分析物的方法和组合物，其中两个或多个所述探针靶向所述分析物的不同区域。 在具体实施方案中，标签是分开区分的，和/或以不同的特定活性存在于标签上。

    Hybridization protection assay
    43.
    发明授权
    Hybridization protection assay 失效
    杂交保护试验

    公开(公告)号：US6004745A

    公开(公告)日：1999-12-21

    申请号：US465435

    申请日：1995-06-05

    申请人： Lyle J. Arnold, Jr. ,  Norman C. Nelson

    发明人： Lyle J. Arnold, Jr. ,  Norman C. Nelson

    IPC分类号： C12Q1/68 ,  G01N33/53 ,  G01N33/532 ,  G01N33/533 ,  G01N33/542 ,  G01N33/58

    CPC分类号： G01N33/58 ,  C12Q1/6816 ,  C12Q1/6823 ,  G01N33/5306 ,  G01N33/532 ,  G01N33/533 ,  G01N33/542

    摘要： Improved homogenous diagnostic assay methods and labels for detecting an analyte in a medium when the analyte is a member of a specific binding pair. The methods and labels provide procedures for reducing background and increasing sensitivity. The binding partner of the analyte is labeled with a substance, the stability of which detectably changes whenever said analyte is bound as a member of the specific binding pair, In a closely related system, the analyte is labeled with a substance susceptible to differential degradation depending on whether or not the analyte is bound as a member of its specific binding pair. After incubation and selective degradation or chemical or biochemical alteration, the amount of analyte bound is detected by measuring either the stability change or the extent of degradation of the label. In a particular system, chemiluminescent acridinium ester labeled probes are used in a homogenous hybridization assay format for sensitively detecting the presence of complement any target polynucleotide sequences.

    摘要翻译： 当分析物是特异性结合对的成员时，改进的同源诊断测定方法和用于检测培养基中分析物的标记。 方法和标签提供了降低背景和提高灵敏度的程序。 分析物的结合配偶体用物质标记，每当所述分析物作为特异性结合对的成员结合时，其稳定性可检测地改变。在紧密相关的系统中，分析物用易受差异降解的物质标记 关于分析物是否作为其特异性结合对的成员结合。 孵育和选择性降解或化学或生物化学改变后，通过测量标签的稳定性变化或降解程度来检测分析物的结合量。 在特定系统中，化学发光的吖啶酯标记的探针以均质杂交测定形式使用，以敏感地检测补体存在任何靶多核苷酸序列。

    Branched nucleic acid probes
    44.
    发明授权
    Branched nucleic acid probes 失效
    支链核酸探针

    公开(公告)号：US5424413A

    公开(公告)日：1995-06-13

    申请号：US940652

    申请日：1992-09-04

    申请人： James J. Hogan ,  Lyle J. Arnold, Jr. ,  Norman C. Nelson ,  Robert Bezverkov

    发明人： James J. Hogan ,  Lyle J. Arnold, Jr. ,  Norman C. Nelson ,  Robert Bezverkov

    IPC分类号： C12N15/09 ,  C07H21/04 ,  C12N15/10 ,  C12Q1/68 ,  C12R1/36

    CPC分类号： C12N15/1068 ,  C12Q1/6816 ,  C12Q1/6823 ,  Y10T436/143333

    摘要： Nucleic acid hybridization probes having at least one nucleic acid strand which has at least two separate target specific regions that hybridize to a target nucleic acid sequence, and at least two distinct arm regions that do not hybridize with the target nucleic acid but possess complementary regions that are capable of hybridizing with one another. These regions are designed such that, under appropriate hybridization conditions, the complementary arm regions will not hybridize to one another in the absence of the target nucleic acid; but, in the presence of target nucleic acid the target-specific regions of the probe will anneal to the target nucleic acid, and the complementary arm regions will anneal to one another, thereby forming a branched nucleic acid structure.

    摘要翻译： 具有至少一个核酸链的核酸杂交探针，其具有与靶核酸序列杂交的至少两个分离的靶特异性区域，以及至少两个不与靶核酸杂交但具有互补区域的不同臂区域， 能够彼此杂交。 这些区域被设计成使得在适当的杂交条件下，互补臂区不会在不存在靶核酸的情况下彼此杂交; 但是在目标核酸的存在下，探针的靶特异性区域将与靶核酸退火，并且互补臂区域将彼此退火，由此形成支化核酸结构。

    Methods for Amplifying Fragmented Target Nucleic Acids Utilizing an Assembler Sequence
    46.
    发明申请
    Methods for Amplifying Fragmented Target Nucleic Acids Utilizing an Assembler Sequence 审中-公开
    使用汇编序列扩增分离的靶核酸的方法

    公开(公告)号：US20160068900A1

    公开(公告)日：2016-03-10

    申请号：US14773366

    申请日：2014-03-15

    申请人： Lyle J. ARNOLD ,  Norman C. NELSON

    发明人： Lyle J. Arnold ,  Norman C. Nelson

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6858 ,  C12Q1/6806 ,  C12Q1/6844 ,  C12Q2525/161 ,  C12Q2525/186 ,  C12Q2525/204 ,  C12Q2531/101

    摘要： The present invention provides methods of amplifying a fragmented target nucleic acid containing short target nucleic acid fragments utilizing an assembler sequence to convert these short fragments into longer sequences enabling their identification and interrogation. This is particularly important when attempting to identify small genetic variations, such as SNVs, present in highly fragmented nucleic acid samples. Amplification is accomplished by hybridizing the short target nucleic acid sequences to the assembler sequence, where these short sequences serve as primers for extension. Since the fragmented target nucleic acids that contain SNVs are utilized as primers on the assembler sequence they are preserved during amplification and can be detected.

    摘要翻译： 本发明提供了利用汇编序列扩增含有短靶核酸片段的片段化靶核酸的方法，以将这些短片段转化成更长的序列，使其能够进行鉴定和询问。 当试图鉴定存在于高度片段化的核酸样品中的小的遗传变异（例如SNV）时，这尤其重要。 通过将短目标核酸序列杂交到汇编序列来完成扩增，其中这些短序列用作扩增的引物。 由于含有SNV的片段化靶核酸作为汇编序列上的引物，因此在扩增过程中被保留并可以被检测。

    Composition kits and methods for performing amplification reactions
    50.
    发明授权
    Composition kits and methods for performing amplification reactions 有权
    用于进行扩增反应的组合物试剂盒和方法

    公开(公告)号：US07696337B2

    公开(公告)日：2010-04-13

    申请号：US11574307

    申请日：2005-08-26

    申请人： Michael M. Becker ,  Steven T. Brentano ,  Daniel P. Kolk ,  Wai-Chung Lam ,  Kristin W. Livezey ,  Norman C. Nelson ,  Astrid R. W. Schroder ,  Gary P. Schroth

    发明人： Michael M. Becker ,  Steven T. Brentano ,  Daniel P. Kolk ,  Wai-Chung Lam ,  Kristin W. Livezey ,  Norman C. Nelson ,  Astrid R. W. Schroder ,  Gary P. Schroth

    IPC分类号： C07H21/04 ,  C12Q1/68

    CPC分类号： C12P19/34 ,  C12Q1/6865 ,  C12Q2537/163 ,  C12Q2521/325 ,  C12Q2521/107 ,  C12Q2525/186 ,  C12Q2533/101 ,  C12Q2525/143

    摘要： The present invention is directed to novel methods of synthesizing multiple copies of a target nucleic acid sequence which are autocatalytic (i.e., able to cycle automatically without the need to modify reaction conditions such as temperature, pH, or ionic strength and using the product of one cycle in the next one). In particular, the present invention discloses a method of nucleic acid amplification which is robust and efficient, while reducing the appearance of side products. The method uses only one primer, the “priming oligonucleotide,” a 3′blocked promoter oligonucleotide and optionally, a means for terminating a primer extension reaction, to amplify RNA or DNA molecules in vitro, while reducing or eliminating the formation of side products. The method of the present invention minimizes or eliminates the emergence of side products, thus providing a high level of specificity. Furthermore, the appearance of side products can complicate the analysis of the amplification reaction by various molecular detection techniques. The present invention minimizes or eliminates this problem, thus providing an enhanced level of sensitivity.

    摘要翻译： 本发明涉及合成目标核酸序列的多个拷贝的新型方法，所述目标核酸序列是自动催化的（即，能够自动循环而不需要修饰诸如温度，pH或离子强度的反应条件，并且使用一种 循环在下一个）。 特别地，本发明公开了一种鲁棒有效的核酸扩增方法，同时减少了副产物的外观。 该方法仅使用一种引物，即引物寡核苷酸，3'封闭的启动子寡核苷酸和任选的终止引物延伸反应的手段，以体外扩增RNA或DNA分子，同时减少或消除副产物的形成。 本发明的方法使副产物的出现最小化或消除，从而提供高水平的特异性。 此外，副产物的出现可能通过各种分子检测技术使扩增反应的分析复杂化。 本发明使这个问题最小化或消除了这一点，从而提供了增强的灵敏度。