公开(公告)号：US09951092B2

公开(公告)日：2018-04-24

申请号：US13580884

申请日：2011-05-20

Applicant: Pamela C. Iraneta ,  Thomas H. Walter ,  Xin Zhang ,  Daniel P. Walsh ,  Kevin D. Wyndham ,  Raymond P. Fisk

Inventor： Pamela C. Iraneta ,  Thomas H. Walter ,  Xin Zhang ,  Daniel P. Walsh ,  Kevin D. Wyndham ,  Raymond P. Fisk

IPC: C07F9/10 ,  B01D15/32 ,  B01J20/28 ,  B01J20/287 ,  B01J20/32 ,  B01J20/10 ,  B01D15/22 ,  B01L3/00 ,  C07K1/30 ,  G01N30/88

CPC classification number: C07F9/103 ,  B01D15/22 ,  B01D15/327 ,  B01J20/103 ,  B01J20/28004 ,  B01J20/28019 ,  B01J20/28061 ,  B01J20/28069 ,  B01J20/287 ,  B01J20/3204 ,  B01J20/3217 ,  B01J20/3246 ,  B01J20/3293 ,  B01J2220/64 ,  B01L3/5023 ,  B01L3/50255 ,  B01L2300/0681 ,  C07K1/30 ,  G01N2030/8813

Abstract: Methods, kits and devices for separating phospholipids and proteins from small molecules in biochemical samples can feature an apparatus having a wetting barrier, at least one fit and a separation media. For example, an apparatus can include at least one wall defining a chamber having an exit and an entrance; a wetting barrier disposed between the exit and entrance, so as to define a separation media space located between the wetting barrier and the exit and a sample receiving area located between the wetting barrier and the entrance; and a separation media disposed adjacent to the wetting barrier and having a specific affinity for phospholipids. The wetting barrier is adapted to (i) retain the liquid sample and a protein precipitating agent in the sample receiving area under a first force, thereby facilitating the formation of a protein precipitate and a processed sample, and (ii) flow the processed sample through the wetting barrier and separation media under a second force, wherein the second force is greater than the first force, thereby retaining the protein precipitate in the sample receiving area, retaining phospholipids in the separation media, and eluting small molecules.

公开(公告)号：US09527059B2

公开(公告)日：2016-12-27

申请号：US14660153

申请日：2015-03-17

Applicant: THE FLORIDA INTERNATIONAL UNIVERSITY BOARD OF TRUSTEES

Inventor： Abuzar Kabir ,  Kenneth G. Furton

IPC: B01L3/00 ,  B01L99/00 ,  B01J20/32 ,  B01J20/286 ,  G01N1/40 ,  G01N1/02

CPC classification number: B01J20/3212 ,  B01J20/286 ,  B01J20/3204 ,  B01J20/321 ,  B01J20/3276 ,  B01J20/328 ,  B01J20/3285 ,  B01J2220/64 ,  G01N1/405 ,  G01N2001/027

Abstract: A field kit for collecting analytical samples has one or more dynamic fabric phase sorptive extraction (DFPSE) devices and/or fabric phase sorptive extraction (FPSE) devices and a plurality of containers for storing and transporting the DFPSEs and/or FPSEs that were used for sampling. The field kit has media for documenting information concerning the site, quantity, date, and/or other pertinent information concerning the sampling. Samples can be maintained within the kit for any required period of storage. Sampling can be done once or a plurality of times, such that an initial analysis can be carried out and analysis can be repeated using a portion of a FPSE or with a redundant FPSE that has been stored. The DFPSE device is a sampling device including a plurality of FPSEs, such that a number of different types of analytes can be sampled in different layers of the DFPSE. At least one external surface layer of the DFPSE is a barrier FPSE that restricts solids from underlying layers.

Abstract translation: 用于收集分析样品的现场试剂盒具有一个或多个动态织物相吸附萃取（DFPSE）装置和/或织物相吸附提取（FPSE）装置和用于储存和运输用于DFPSE和/或FPSE的DFPSE和/或FPSE的多个容器 采样。 现场工具包有媒体记录有关采样的站点，数量，日期和/或其他相关信息的信息。 样品可以保存在试剂盒中以保持任何所需的储存期。 可以进行一次或多次采样，使得可以执行初始分析并且可以使用FPSE的一部分或已经存储的冗余FPSE来重复分析。 DFPSE设备是包括多个FPSE的采样设备，使得可以在DFPSE的不同层中对多个不同类型的分析物进行采样。 DFPSE的至少一个外表面层是限制来自下层的固体的屏障FPSE。

公开(公告)号：US09114383B2

公开(公告)日：2015-08-25

申请号：US10920922

申请日：2004-08-17

Applicant: Douglas T. Gjerde ,  Ronald Jones ,  Allen Burge ,  Christopher P. Hanna ,  Leon S. Yengoyan

Inventor： Douglas T. Gjerde ,  Ronald Jones ,  Allen Burge ,  Christopher P. Hanna ,  Leon S. Yengoyan

IPC: G01N30/02 ,  G01N30/56 ,  B01J20/285 ,  B01J20/286 ,  B01L3/02 ,  B82Y30/00 ,  B01D15/22 ,  G01N1/40 ,  G01N35/10

CPC classification number: B01J20/285 ,  B01D15/22 ,  B01J20/286 ,  B01J2220/54 ,  B01J2220/58 ,  B01J2220/64 ,  B01L3/0275 ,  B01L2200/0631 ,  B82Y30/00 ,  G01N1/405 ,  G01N30/02 ,  G01N30/56 ,  G01N2035/1053 ,  B01D15/3804

Abstract: The invention provides extraction columns for the purification of an analyte (e.g., a biological macromolecule, such as a peptide, protein or nucleic acid) from a sample solution, as well as methods for making and using such columns. The columns typically include a bed of extraction media positioned in the column between two frits. In some embodiments, the extraction columns employ modified pipette tips as column bodies. In some embodiments, the invention provides methods of storing columns in a wet state.

Abstract translation: 本发明提供了用于从样品溶液中纯化分析物（例如生物大分子，如肽，蛋白质或核酸）的提取柱，以及制备和使用这些色谱柱的方法。 柱通常包括位于两个玻璃料之间的柱中的提取介质床。 在一些实施方案中，提取柱使用改进的移液管尖作为柱体。 在一些实施方案中，本发明提供了在湿状态下储存塔的方法。

公开(公告)号：US08956445B2

公开(公告)日：2015-02-17

申请号：US13435163

申请日：2012-03-30

Applicant: Daniel W. Armstrong ,  Jared Anderson

Inventor： Daniel W. Armstrong ,  Jared Anderson

IPC: B01D53/30 ,  B01J20/292 ,  B01J20/22 ,  B01J20/286 ,  B01J20/32 ,  C07D233/54 ,  C07F9/54 ,  C07F9/58 ,  C07F9/6512 ,  B01J20/29 ,  B01J20/30

CPC classification number: B01J20/292 ,  B01J20/22 ,  B01J20/286 ,  B01J20/29 ,  B01J20/3092 ,  B01J20/3204 ,  B01J20/3246 ,  B01J20/3248 ,  B01J20/3251 ,  B01J20/3253 ,  B01J20/3255 ,  B01J20/3276 ,  B01J20/3282 ,  B01J20/3285 ,  B01J20/3287 ,  B01J2220/54 ,  B01J2220/58 ,  B01J2220/64 ,  B01J2220/84 ,  C07D233/54 ,  C07F9/5407 ,  C07F9/5449 ,  C07F9/58 ,  C07F9/6512 ,  Y02P20/542

Abstract: The present invention relates to diionic liquid salts of dicationic or dianionic molecules, as well as solvents comprising such diionic liquids and the use of such diionic liquids as the stationary phase in a gas chromatographic column.

Abstract translation: 本发明涉及双阳离子或二阴离子分子的双离子液体盐，以及包含这种双离子液体的溶剂以及在气相色谱柱中使用这种二元液体作为固定相。

公开(公告)号：US08685283B2

公开(公告)日：2014-04-01

申请号：US12201796

申请日：2008-08-29

Applicant: Ta-Chen Wei ,  Wu Chen ,  William E. Barber

Inventor： Ta-Chen Wei ,  Wu Chen ,  William E. Barber

IPC: G01N33/00 ,  C01B33/26

CPC classification number: C09C1/3063 ,  B01J20/08 ,  B01J20/103 ,  B01J20/28004 ,  B01J20/28057 ,  B01J20/28078 ,  B01J20/286 ,  B01J20/3204 ,  B01J20/3246 ,  B01J20/3257 ,  B01J20/3293 ,  B01J20/3295 ,  B01J2220/64 ,  C01B33/18 ,  C01F7/021 ,  C01P2002/72 ,  C01P2004/03 ,  C01P2004/52 ,  C01P2004/61 ,  C01P2004/62 ,  C01P2006/12 ,  C01P2006/16 ,  C01P2006/17 ,  C09C1/3669 ,  C09C1/407

Abstract: Micelle-templated superficially porous particles having a solid core and an outer porous shell with ordered pore structures and a narrow particle size distribution, such as about ±5% (one sigma), and a high specific surface area of about 5 to about 1000 m2/g.

Abstract translation: 具有实心芯和具有有序孔结构和窄粒度分布的外多孔壳（例如约±5％（一西格玛））和约5至约1000m 2的高比表面积的胶束模板表面多孔颗粒 /G。

公开(公告)号：US20130153479A1

公开(公告)日：2013-06-20

申请号：US13764394

申请日：2013-02-11

Applicant: INTRINSIC BIOPROBES, INC.

Inventor： Kemmons Tubbs ,  Karl F. Gruber ,  Randall W. Nelson

IPC: G01N33/68

CPC classification number: G01N33/6845 ,  B01J20/28097 ,  B01J20/281 ,  B01J20/286 ,  B01J20/32 ,  B01J20/3204 ,  B01J20/3208 ,  B01J20/3242 ,  B01J20/3259 ,  B01J20/3265 ,  B01J20/3272 ,  B01J20/3274 ,  B01J20/3282 ,  B01J2220/54 ,  B01J2220/64 ,  B82Y30/00 ,  G01N30/02 ,  G01N30/48 ,  G01N30/6095 ,  G01N33/54366 ,  G01N33/545 ,  G01N33/6803 ,  G01N2570/00 ,  H01J49/164 ,  H01J49/40 ,  Y10T436/255 ,  B01D15/3804

Abstract: Described is an affinity microcolumn comprising a high surface area material, which has high flow properties and a low dead volume, contained within a housing and having affinity reagents bound to the surface of the high surface area material that are either activated or activatable. The affinity reagents bound to the surface of the affinity microcolumn further comprise affinity receptors for the integration into high throughput analysis of biomolecules.

Abstract translation: 描述了一种亲和力微柱，其包含高表面积材料，其具有包含在壳体内的高流动性和低死体积，并且具有结合到高表面积材料的表面的亲和试剂，其被激活或可激活。 结合亲和微柱表面的亲和试剂还包含用于整合生物分子的高通量分析的亲和受体。

公开(公告)号：US08336403B2

公开(公告)日：2012-12-25

申请号：US12448689

申请日：2008-01-21

Applicant: Hideji Tajima

Inventor： Hideji Tajima

IPC: B01L3/02

CPC classification number: B01L3/0275 ,  B01D15/3804 ,  B01J2220/64 ,  B01L2200/141 ,  B01L2200/16 ,  B01L2300/042 ,  B01L2300/044 ,  B01L2300/0636 ,  B01L2300/0681 ,  B01L2300/069 ,  G01N30/6043 ,  G01N2035/1053

Abstract: The present invention relates to a pipette tip having a support and a fluid enclosed therein, an apparatus for treating the pipette tip having a support and a fluid enclosed therein, and a method of treating the pipette tip having a support and a fluid enclosed therein. The present invention aims at providing separation and purification that may be performed more efficiently and rapidly than the treatments using a conventional treatment using liquid chromatograph or a filter, and constituted to have a function for sustainedly activating the support by comprising a pipette tip comprising an attachment opening that is to be attached to a nozzle for sucking and discharging a gas or to a connecting tube attachable to the nozzle and may be communicated with the nozzle, and an opening that allows flow-in and flow-out of a liquid in response to the suction and discharging of the gas; a support enclosed in the pipette tip, which may adsorb or capture a biological material in the liquid or react with or bond to the biological material; and a fluid for sustainedly activating the support, which comprises a predetermined liquid or a predetermined gas that is enclosed in the pipette tip in a breakable state and comes into contact with the support.

Abstract translation: 本发明涉及具有支撑体和封闭在其中的流体的移液管尖端，用于处理具有支撑件和封闭在其中的流体的移液管尖端的装置，以及处理具有支撑件的移液管尖端和封闭在其中的流体的方法。 本发明旨在提供可以比使用液相色谱仪或过滤器的常规处理的处理更有效和快速地进行的分离和纯化，并且构成为具有通过包括附着物的移液管尖端来持续活化载体的功能 要连接到用于吸入和排出气体的喷嘴的开口或连接到喷嘴的连接管，并且可以与喷嘴连通，以及允许响应于流体流动而流入和流出液体的开口 气体的吸入和排出; 包封在移液管尖端的支架，其可以吸附或捕获液体中的生物材料或与生物材料反应或结合到生物材料上; 以及用于持续地启动支撑件的流体，其包括预定液体或预定的气体，所述预定液体或预定气体以可破坏的状态包封在吸头中并与支撑体接触。

公开(公告)号：US08182581B2

公开(公告)日：2012-05-22

申请号：US11187389

申请日：2005-07-22

Applicant: Daniel W. Armstrong ,  Jared Anderson

Inventor： Daniel W. Armstrong ,  Jared Anderson

IPC: B01D53/02 ,  C07D233/00 ,  C07D207/18

CPC classification number: C07F9/72 ,  B01J20/22 ,  B01J20/28097 ,  B01J20/286 ,  B01J20/292 ,  B01J20/3204 ,  B01J20/3287 ,  B01J2220/54 ,  B01J2220/64 ,  B01J2220/84 ,  C07C211/09 ,  C07D233/54 ,  C07D333/46

Abstract: The present invention relates to diionic liquid salts of dicationic or dianionic molecules, as well as solvents comprising diionic liquids and the use of diionic liquids as the stationary phase in a gas chromatographic column.

Abstract translation: 本发明涉及双阳离子或二阴离子分子的双离子液体盐，以及包含双离子液体的溶剂以及在气相色谱柱中使用双离子液体作为固定相。

公开(公告)号：US20120085692A1

公开(公告)日：2012-04-12

申请号：US13315163

申请日：2011-12-08

Applicant: Kemmons A. Tubbs ,  Karl F. Gruber ,  Randall W. Nelson

Inventor： Kemmons A. Tubbs ,  Karl F. Gruber ,  Randall W. Nelson

IPC: B01D15/08

CPC classification number: G01N33/6845 ,  B01J20/28097 ,  B01J20/281 ,  B01J20/286 ,  B01J20/32 ,  B01J20/3204 ,  B01J20/3208 ,  B01J20/3242 ,  B01J20/3259 ,  B01J20/3265 ,  B01J20/3272 ,  B01J20/3274 ,  B01J20/3282 ,  B01J2220/54 ,  B01J2220/64 ,  B82Y30/00 ,  G01N30/02 ,  G01N30/48 ,  G01N30/6095 ,  G01N33/54366 ,  G01N33/545 ,  G01N33/6803 ,  G01N2570/00 ,  H01J49/164 ,  H01J49/40 ,  Y10T436/255 ,  B01D15/3804

Abstract: Described is an affinity microcolumn comprising a high surface area material, which has high flow properties and a low dead volume, contained within a housing and having affinity reagents bound to the surface of the high surface area material that are either activated or activatable. The affinity reagents bound to the surface of the affinity microcolumn further comprise affinity receptors for the integration into high throughput analysis of biomolecules.

Abstract translation: 描述了一种亲和力微柱，其包含高表面积材料，其具有包含在壳体内的高流动性和低死体积，并且具有结合到高表面积材料的表面的亲和试剂，其被激活或可激活。 结合亲和微柱表面的亲和试剂还包含用于整合生物分子的高通量分析的亲和受体。

公开(公告)号：US08143071B2

公开(公告)日：2012-03-27

申请号：US12004726

申请日：2007-12-21

Applicant: Douglas T. Gjerde

Inventor： Douglas T. Gjerde

IPC: G01N33/543

CPC classification number: C07K1/22 ,  B01J2220/64 ,  G01N30/02 ,  G01N30/461 ,  G01N30/466 ,  G01N2030/009 ,  G01N2030/085 ,  B01D15/3804 ,  B01D15/361 ,  B01D15/1871

Abstract: The invention provides columns and methods for the purification and concentration of an analyte (e.g., a biological macromolecule, such as a peptide, protein or nucleic acid) from a sample solution. The columns typically include a bed of extraction medium positioned in the column between two frits. In some embodiments, the extraction columns employ modified pipette tips as column bodies. The invention also provides methods for purifying and concentrating multiple analytes simultaneously.

Abstract translation: 本发明提供了用于从样品溶液纯化和浓缩分析物（例如，生物大分子，例如肽，蛋白质或核酸）的柱和方法。 柱通常包括位于两个玻璃料之间的柱中的提取介质床。 在一些实施方案中，提取柱使用改进的移液管尖作为柱体。 本发明还提供了同时净化和浓缩多种分析物的方法。