公开(公告)号：US20090260988A1

公开(公告)日：2009-10-22

申请号：US12494927

申请日：2009-06-30

Applicant: Vamsee K. Pamula ,  Michael G. Pollack ,  Philip Y. Paik ,  Hong Ren ,  Richard B. Fair

Inventor： Vamsee K. Pamula ,  Michael G. Pollack ,  Philip Y. Paik ,  Hong Ren ,  Richard B. Fair

IPC: B01D57/02

CPC classification number: B01L3/50273 ,  B01F11/0071 ,  B01F13/0071 ,  B01F13/0076 ,  B01F13/0084 ,  B01L3/00 ,  B01L3/5027 ,  B01L3/502784 ,  B01L3/502792 ,  B01L2200/06 ,  B01L2200/0605 ,  B01L2200/10 ,  B01L2300/089 ,  B01L2300/161 ,  B01L2300/165 ,  B01L2400/0415 ,  B01L2400/0427 ,  B41J2002/14395 ,  C25B9/00 ,  G01N27/44791 ,  G02B26/005

Abstract: Methods are provided for manipulating droplets. The methods include providing the droplet on a surface comprising an array of electrodes and a substantially co-planer array of reference elements, wherein the droplet is disposed on a first one of the electrodes, and the droplet at least partially overlaps a second one of the electrodes and an intervening one of the reference elements disposed between the first and second electrodes. The methods further include activating the first and second electrodes to spread at least a portion of the droplet across the second electrode and deactivating the first electrode to move the droplet from the first electrode to the second electrode.

Abstract translation: 提供了用于操纵液滴的方法。 所述方法包括在包括电极阵列和基本上共平面的参考元件阵列的表面上提供液滴，其中液滴设置在电极的第一个上，并且液滴至少部分地与第二个 电极和设置在第一和第二电极之间的参考元件中的插入件之一。 所述方法还包括激活第一和第二电极以将液滴的至少一部分扩散穿过第二电极，并且去激活第一电极以使液滴从第一电极移动到第二电极。

公开(公告)号：US07569129B2

公开(公告)日：2009-08-04

申请号：US11077569

申请日：2005-03-10

Applicant: Vamsee K. Pamula ,  Michael G. Pollack ,  Philip Y. Paik ,  Hong Ren ,  Richard B. Fair

Inventor： Vamsee K. Pamula ,  Michael G. Pollack ,  Philip Y. Paik ,  Hong Ren ,  Richard B. Fair

IPC: F04B19/00 ,  B81B7/00

CPC classification number: B01L3/50273 ,  B01F11/0071 ,  B01F13/0071 ,  B01F13/0076 ,  B01F13/0084 ,  B01L3/00 ,  B01L3/5027 ,  B01L3/502784 ,  B01L3/502792 ,  B01L2200/06 ,  B01L2200/0605 ,  B01L2200/10 ,  B01L2300/089 ,  B01L2300/161 ,  B01L2300/165 ,  B01L2400/0415 ,  B01L2400/0427 ,  B41J2002/14395 ,  C25B9/00 ,  G01N27/44791 ,  G02B26/005

Abstract: Methods are provided for manipulating droplets. The methods include providing the droplet on a surface comprising an array of electrodes and a substantially co-planer array of reference elements, wherein the droplet is disposed on a first one of the electrodes, and the droplet at least partially overlaps a second one of the electrodes and an intervening one of the reference elements disposed between the first and second electrodes. The methods further include activating the first and second electrodes to spread at least a portion of the droplet across the second electrode and deactivating the first electrode to move the droplet from the first electrode to the second electrode.

Abstract translation: 提供了用于操纵液滴的方法。 所述方法包括在包括电极阵列和基本上共平面的参考元件阵列的表面上提供液滴，其中液滴设置在电极的第一个上，并且液滴至少部分地与第二个 电极和设置在第一和第二电极之间的参考元件中的插入件之一。 所述方法还包括激活第一和第二电极以将液滴的至少一部分扩散穿过第二电极，并且去激活第一电极以使液滴从第一电极移动到第二电极。

公开(公告)号：US20080264797A1

公开(公告)日：2008-10-30

申请号：US11965206

申请日：2007-12-27

Applicant: Vamsee K. Pamula ,  Michael G. Pollack ,  Richard B. Fair

Inventor： Vamsee K. Pamula ,  Michael G. Pollack ,  Richard B. Fair

IPC: G01N27/00

CPC classification number: B01L3/50273 ,  B01F11/0071 ,  B01F13/0071 ,  B01F13/0076 ,  B01F13/0084 ,  B01L3/00 ,  B01L3/5027 ,  B01L3/502784 ,  B01L3/502792 ,  B01L2200/06 ,  B01L2200/0605 ,  B01L2200/10 ,  B01L2300/089 ,  B01L2300/161 ,  B01L2300/165 ,  B01L2400/0415 ,  B01L2400/0427 ,  B41J2002/14395 ,  C25B9/00 ,  G01N27/44791 ,  G02B26/005

Abstract: An apparatus for manipulating droplets is provided. In one embodiment, the apparatus includes a substrate having a set of electrical leads for connecting electrodes to a controller, a first set of electrodes, each connected to a separate one of the electrical leads, and a second set of electrodes, all connected to a single one of the electrical leads. In another embodiment, the apparatus includes a substrate having a set of X electrodes, and a set of Y electrical leads, each connected to one or more electrodes, wherein X is greater than Y.

Abstract translation: 提供了一种用于操纵液滴的装置。 在一个实施例中，该装置包括具有用于将电极连接到控制器的一组电引线的基板，分别连接到电引线的单独电极的第一组电极和全部连接到 单一电引线。 在另一个实施例中，该装置包括具有一组X电极的基板和一组Y电引线，每组连接到一个或多个电极，其中X大于Y.

公开(公告)号：US20080247920A1

公开(公告)日：2008-10-09

申请号：US11965152

申请日：2007-12-27

Applicant: Michael G. Pollack ,  Vamsee K. Pamula ,  Richard B. Fair

Inventor： Michael G. Pollack ,  Vamsee K. Pamula ,  Richard B. Fair

IPC: B01J19/08

CPC classification number: B01L3/50273 ,  B01F11/0071 ,  B01F13/0071 ,  B01F13/0076 ,  B01F13/0084 ,  B01L3/00 ,  B01L3/5027 ,  B01L3/502784 ,  B01L3/502792 ,  B01L2200/06 ,  B01L2200/0605 ,  B01L2200/10 ,  B01L2300/089 ,  B01L2300/161 ,  B01L2300/165 ,  B01L2400/0415 ,  B01L2400/0427 ,  B41J2002/14395 ,  C25B9/00 ,  G01N27/44791 ,  G02B26/005

Abstract: An apparatus for manipulating droplets is provided. In one embodiment, the apparatus includes a substrate having electrodes and adjacent reference elements configured for manipulating a droplet on a surface of the substrate. Other embodiments having a top plate, a footprint, and a dielectric are also included.

Abstract translation: 提供了一种用于操纵液滴的装置。 在一个实施例中，该装置包括具有电极和相邻参考元件的基板，该参考元件被配置用于在基板的表面上操纵液滴。 还包括具有顶板，覆盖区和电介质的其它实施例。

公开(公告)号：US20080213766A1

公开(公告)日：2008-09-04

申请号：US11837581

申请日：2007-08-13

Applicant: James F. BROWN ,  Jonathan E. SILVER

Inventor： James F. BROWN ,  Jonathan E. SILVER

IPC: C12Q1/68

CPC classification number: C12Q1/6806 ,  B01J2219/00317 ,  B01J2219/00621 ,  B01J2219/00637 ,  B01J2219/00644 ,  B01J2219/00659 ,  B01J2219/00677 ,  B01J2219/00722 ,  B01L3/5027 ,  B01L3/5085 ,  B01L3/50851 ,  B01L3/5088 ,  B01L2200/06 ,  B01L2200/0642 ,  B01L2200/10 ,  B01L2200/16 ,  B01L2300/0636 ,  B01L2300/0819 ,  B01L2400/0409 ,  C07H21/00 ,  C12Q1/6844 ,  C12Q1/686 ,  C40B60/14 ,  Y10S436/805 ,  Y10S436/809 ,  Y10T436/2575

Abstract: A method comprising for each individual sample of a plurality of samples, loading at least one sample portion of the individual sample into at least one respective sample chamber of a plurality of sample chambers, subjecting the sample portions to at least a first amplification step; and then determining whether sample portions contain at least one molecule of the target nucleic acid. For each sample portion, if the sample portion contains at least a single molecule of the target nucleic acid, the sample portion would attain a detectable concentration of the target nucleic acid after a single round of amplification.

Abstract translation: 一种方法，其包括对多个样品的每个单个样品进行加样，将所述单个样品的至少一个样品部分加载到多个样品室的至少一个相应的样品室中，使所述样品部分至少进行第一扩增步骤; 然后确定样品部分是否含有至少一个分子的靶核酸。 对于每个样品部分，如果样品部分含有至少一个单一分子的靶核酸，则样品部分将在单次扩增后获得可检测的靶核酸浓度。

公开(公告)号：US07163612B2

公开(公告)日：2007-01-16

申请号：US10305429

申请日：2002-11-26

Applicant: James D. Sterling ,  Chao-Yi Chen

Inventor： James D. Sterling ,  Chao-Yi Chen

IPC: G01N27/26

CPC classification number: B01L3/50273 ,  B01L3/502715 ,  B01L3/502738 ,  B01L3/502792 ,  B01L2200/06 ,  B01L2300/0819 ,  B01L2300/089 ,  B01L2400/0415 ,  B01L2400/0427 ,  F04B17/00 ,  F04B19/006 ,  G02B26/005

Abstract: An active matrix microfluidic platform employs thin film transistor active (“TFT”) matrix liquid crystal display technology to manipulate small samples of fluid for chemical, biochemical, or biological assays without moving parts, for example, using a two-dimensional matrix array of drive electrodes. The active matrix microfluidic platform may employ existing active matrix addressing schemes and/or commercial “off-the-shelf” animation software to program assay protocols. A feedback subsystem may determine an actual location of a fluid in the microfluidic structure, and provides location information to for display, for example, on an active matrix display, and/or to control movement of one or more fluid bodies in the microfluidic structure.

Abstract translation: 有源矩阵微流体平台采用薄膜晶体管有源（“TFT”）矩阵液晶显示技术来操纵用于化学，生物化学或生物测定的小样本的液体，无需移动部件，例如使用驱动器的二维矩阵阵列 电极。 有源矩阵微流体平台可以采用现有的有源矩阵寻址方案和/或商业“现成”动画软件来编程测定方案。 反馈子系统可以确定微流体结构中的流体的实际位置，并且提供位置信息以用于例如在有源矩阵显示器上显示，和/或控制微流体结构中的一个或多个流体体的运动。

公开(公告)号：US20060054503A1

公开(公告)日：2006-03-16

申请号：US11077569

申请日：2005-03-10

Applicant: Vamsee Pamula ,  Michael Pollack ,  Philip Paik ,  Hong Ren ,  Richard Fair

Inventor： Vamsee Pamula ,  Michael Pollack ,  Philip Paik ,  Hong Ren ,  Richard Fair

IPC: C07K1/26 ,  G01N27/447

CPC classification number: B01L3/50273 ,  B01F11/0071 ,  B01F13/0071 ,  B01F13/0076 ,  B01F13/0084 ,  B01L3/00 ,  B01L3/5027 ,  B01L3/502784 ,  B01L3/502792 ,  B01L2200/06 ,  B01L2200/0605 ,  B01L2200/10 ,  B01L2300/089 ,  B01L2300/161 ,  B01L2300/165 ,  B01L2400/0415 ,  B01L2400/0427 ,  B41J2002/14395 ,  C25B9/00 ,  G01N27/44791 ,  G02B26/005

Abstract: Methods are provided for manipulating droplets. The methods include providing the droplet on a surface comprising an array of electrodes and a substantially co-planer array of reference elements, wherein the droplet is disposed on a first one of the electrodes, and the droplet at least partially overlaps a second one of the electrodes and an intervening one of the reference elements disposed between the first and second electrodes. The methods further include activating the first and second electrodes to spread at least a portion of the droplet across the second electrode and deactivating the first electrode to move the droplet from the first electrode to the second electrode.

公开(公告)号：US20040096867A1

公开(公告)日：2004-05-20

申请号：US10472421

申请日：2003-09-19

Inventor： Per Andersson ,  Mats Gannulls ,  Gunnar Thorsnulln

IPC: C12Q001/68 ,  G01N033/53 ,  C12M001/34

CPC classification number: G01N33/54306 ,  B01F5/0646 ,  B01F5/0647 ,  B01F5/0655 ,  B01F13/0059 ,  B01J19/0093 ,  B01J2219/00788 ,  B01J2219/00833 ,  B01J2219/0086 ,  B01J2219/00862 ,  B01J2219/00891 ,  B01L3/5025 ,  B01L3/5027 ,  B01L3/502738 ,  B01L3/502746 ,  B01L2200/06 ,  B01L2200/0605 ,  B01L2200/10 ,  B01L2200/142 ,  B01L2300/069 ,  B01L2300/0803 ,  B01L2300/0806 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2300/14 ,  B01L2300/16 ,  B01L2400/0406 ,  B01L2400/0409 ,  B01L2400/0633 ,  B01L2400/0688 ,  B01L2400/086 ,  G01N30/6095 ,  G01N30/7266 ,  G01N33/54366 ,  G01N35/00069 ,  G01N2035/00504 ,  H01J49/04 ,  Y10S435/973

Abstract: A microscale method for the characterization of one or more reaction variables that influence the formation or dissociation of an affinity complex comprising a ligand and a binder, which have mutual affinity for each other. The method is characterized in comprising the steps of: (i) providing a microfluidic device comprising a microchannel structures that are under a common flow control, each microchannel structure comprising a reaction microactivity; (ii) performing essentially in parallel an experiment in each of two or more of the plurality of microchannel structures, the experiment in these two or more microchannel structures comprising either a) formation of an immobilized form of the complex and retaining under flow conditions said form within the reaction microactivity, or b) dissociating, preferably under flow condition, an immobilized form of the complex which has been included in the microfluidic device provided in step (i), at least one reaction variable varies or is uncharacterized for said two or more microchannel structures while the remaining reaction variables are kept essentially constant; (iii) measuring the presentation of the complex in said reaction microactivity in said two or more microchannel structures; and (iv) characterizing said one or more reaction variables based on the values for presentation obtained in step (iii).

Abstract translation: 用于表征影响包含配体和粘合剂的亲和络合物的形成或解离的一个或多个反应变量的微观方法，其彼此具有相互亲和力。 该方法的特征在于包括以下步骤：（i）提供包含处于共同流量控制下的微通道结构的微流体装置，每个微通道结构包含反应微活性; （ii）在两个或多个多个微通道结构的每一个中基本上并行地进行实验，这两个或更多个微通道结构中的实验包括a）形成复合物的固定形式并在流动条件下保持所述形式 或者b）优选在流动条件下解离包含在步骤（i）中提供的微流体装置中的复合物的固定化形式，至少一个反应变量对于所述两个或更多个变化是变化的或没有表征的 微通道结构，而剩余的反应变量保持基本恒定; （iii）测量所述两个或多个微通道结构中所述反应微活性物中复合物的表现; 和（iv）基于在步骤（iii）中获得的呈现值来表征所述一个或多个反应变量。

公开(公告)号：US20240316561A1

公开(公告)日：2024-09-26

申请号：US18579562

申请日：2022-07-29

Applicant: Beijing BOE Sensor Technology Co., Ltd. ,  BOE TECHNOLOGY GROUP CO., LTD.

Inventor： Bolin Fan ,  Le Gu ,  Yongjia Gao ,  Yingying Zhao ,  Li Yang ,  Huazhe Liu

IPC: B01L3/00

CPC classification number: B01L3/502792 ,  B01L2200/06 ,  B01L2300/0645 ,  B01L2400/0415

Abstract: Provided are a microfluidic substrate, a microfluidic chip, and an assay device. The microfluidic substrate includes: a base substrate; a conductive layer arranged on the base substrate, patterns of the conductive layer includes one or more electrode patterns and one or more trace patterns, an orthogonal projection of at least a portion of each trace pattern onto the base substrate is on one side of an orthogonal projection of a corresponding electrode pattern onto the base substrate with a minimum spacing of greater than or equal to 4 micrometers from an outer contour of the electrode pattern.

公开(公告)号：US12017212B2

公开(公告)日：2024-06-25

申请号：US17013718

申请日：2020-09-07

Applicant: SPF Technologies LLC

Inventor： Theodore McCabe, Jr. ,  Gaston De Los Reyes ,  Amanda Lemire ,  Danielle Marquis

IPC: B01L3/00 ,  B29C64/118 ,  B33Y10/00 ,  B33Y40/20 ,  B33Y80/00 ,  B29L31/14

CPC classification number: B01L3/502707 ,  B29C64/118 ,  B33Y10/00 ,  B33Y40/20 ,  B33Y80/00 ,  B01L2200/06 ,  B01L2200/12 ,  B29L2031/14

Abstract: A fluidic device includes an impermeable base, single-strand walls coupled to the impermeable plate. The single-strand walls include a plurality of loops, each loop has a lower part of a double wedge and an upper part of a double wedge aligned with the lower part of the double wedge. The device also includes a lattice connected to the single-strand wall with a loop-as-wipe connection and a gabbled roof disposed opposite the impermeable base and coupled to the tops of the single-strand walls.