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    Chromatography columns, systems and methods
    52.
    发明授权
    Chromatography columns, systems and methods 有权
    色谱柱，系统和方法

    公开(公告)号：US09527009B2

    公开(公告)日：2016-12-27

    申请号：US14252874

    申请日：2014-04-15

    Applicant: GE HEALTHCARE BIO-SCIENCES AB

    Inventor： Klaus Gebauer

    IPC: G01N30/60 ,  G01N30/38 ,  B01D15/22 ,  B01D15/18 ,  B01D15/26

    CPC classification number: G01N30/6004 ,  B01D15/18 ,  B01D15/22 ,  G01N30/6017 ,  G01N30/6021 ,  G01N30/6052 ,  G01N30/606

    Abstract: The present invention relates to axial flow chromatography columns, methods for separating one or more analytes in a liquid by the use of such columns, and systems employing such columns. The column comprises a first port and a second port, the first port and said second port being at essentially the same level or elevation above the level of the bed space on the chromatography column.

    Abstract translation: 本发明涉及轴流色谱柱，通过使用这种色谱柱分离液体中的一种或多种分析物的方法，以及采用这种色谱柱的系统。 该柱包括第一端口和第二端口，第一端口和第二端口在色谱柱上的床空间的水平以上基本相同的水平或高度。

    Separation method
    54.
    发明授权
    Separation method 有权

    公开(公告)号：US10730908B2

    公开(公告)日：2020-08-04

    申请号：US15798784

    申请日：2017-10-31

    Applicant: GE HEALTHCARE BIOPROCESS R&D AB

    Inventor： Annika Forss ,  Mats Ander ,  Tomas Bjorkman ,  Hans Blom ,  Jesper Hansson ,  Gustav Rodrigo

    IPC: C07K1/16 ,  B01D15/08 ,  C07K1/22 ,  C07K16/00 ,  B01J20/286 ,  B01J20/285 ,  B01D15/18 ,  B01D15/34 ,  C07K14/31

    Abstract: The invention relates to a method of isolating an immunoglobulin, comprising the steps of: a) providing a separation matrix comprising at least 15 mg/ml multimers of immunoglobulin-binding alkali-stabilized Protein A domains covalently coupled to a porous support, wherein the porous support comprises cross-linked polymer particles having a volume-weighted median diameter (d50,v) of 56-70 micrometers and a dry solids weight of 55-80 mg/ml; b) contacting a liquid sample comprising an immunoglobulin with the separation matrix; c) washing the separation matrix with a washing liquid; d) eluting the immunoglobulin from the separation matrix with an elution liquid; and e) cleaning the separation matrix with a cleaning liquid comprising at least 0.5 M NaOH.

    Affinity chromatography matrix
    59.
    发明授权
    Affinity chromatography matrix 有权

    公开(公告)号：US09683013B2

    公开(公告)日：2017-06-20

    申请号：US13996023

    申请日：2011-12-19

    Applicant: Tomas Bjorkman ,  Elin Monie ,  Gustav Rodrigo

    Inventor： Tomas Bjorkman ,  Elin Monie ,  Gustav Rodrigo

    IPC: B01D15/38 ,  B01J20/286 ,  C07K1/22 ,  B01J20/289 ,  C07K14/31 ,  C07K14/745 ,  B01J20/32

    CPC classification number: C07K1/22 ,  B01D15/3809 ,  B01J20/286 ,  B01J20/289 ,  B01J20/3212 ,  B01J20/3219 ,  B01J20/3274 ,  C07K14/31 ,  C07K14/745

    Abstract: The present invention relates to a method of separating one or more immunoglobulin containing proteins from a liquid. The method includes first contacting the liquid with a separation matrix comprising ligands immobilized to a support; allowing the immunoglobulin containing proteins to adsorb to the matrix by interaction with the ligands; followed by an optional step of washing the matrix containing the immunoglobulin containing proteins adsorbed thereon; and recovering said immunoglobulin containing proteins by contacting the matrix with an eluent which releases the proteins. The method improves upon previous separation methods in that each of the ligands comprises one or more of a protein A domain (E, D, A, B, C), or protein Z, or a functional variant thereof, with at least one of the monomers having a substitution of the Asparagine at the position corresponding to N28 of B domain of Protein A or Protein Z, and wherein the ligand provides an increase in elution pH compared to non-substituted ligand.

    Plasma protein fractionation by sequential polyacid precipitation
    60.
    发明授权
    Plasma protein fractionation by sequential polyacid precipitation 有权

    公开(公告)号：US09624262B2

    公开(公告)日：2017-04-18

    申请号：US14345302

    申请日：2012-09-14

    Applicant: James Van Alstine ,  Mikael Berg ,  Johanna Kjorning ,  Jamil Shanagar

    Inventor： James Van Alstine ,  Mikael Berg ,  Johanna Kjorning ,  Jamil Shanagar

    IPC: C07K1/30 ,  C07K14/75 ,  C07K14/76 ,  C07K14/765 ,  C07K16/06 ,  C07K16/18 ,  A61K35/16

    CPC classification number: C07K1/30 ,  A61K35/16 ,  C07K1/303 ,  C07K14/75 ,  C07K14/76 ,  C07K16/18

    Abstract: There is a recognized need for novel, more simplified, approaches to isolation of plasma from whole blood, as well as a need to isolate cell-free plasma fractions containing different plasma proteins. Methods are divulged for use of aqueous phase systems, formed in blood or blood containing solutions via addition of a single polymer at relatively low concentration, to effect isolation (clarification) of plasma proteins from blood cells. Methods are also divulged to replace widely used Cohn-type plasma protein fractionation which is based on sequential addition of up to 40% (v/v) ethanol and other precipitants, with simple sequential addition of a polyacid. The latter results in isolation of plasma protein fractions (i.e. fibrinogen, immunoglobulin, albumin) in sequence similar to that obtained with Cohn Fractionation and therefore may be suitable for use to reduce solvent use and solvent-related process complications in existing plasma protein purification processes. It may also support use of polymeric film based containers in novel solvent free plasma fractionation processes. The methods disclosed may also be suitable for use in smaller scale plasma protein isolation, in research and diagnostic applications. The general methodologies are robust and can function over a broad range of process variables such as temperature and pH.

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