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    Method of Preparing a Nucleic Acid Library
    52.
    发明申请
    Method of Preparing a Nucleic Acid Library 审中-公开
    制备核酸文库的方法

    公开(公告)号：US20150038344A1

    公开(公告)日：2015-02-05

    申请号：US14520736

    申请日：2014-10-22

    Applicant: ADVANCED LIQUID LOGIC, INC.

    Inventor： Michael G. Pollack ,  Allen E. Eckhardt ,  Prasanna Thwar ,  Jeremy Rouse

    IPC: C12N15/10

    CPC classification number: C12N15/1075 ,  B01J19/0046 ,  C12N15/1006 ,  C40B40/08 ,  C40B50/06

    Abstract: A method of preparing a nucleic acid library in droplets in contact with oil, including: (a) blunt-ending nucleic acid fragments in a droplet in the oil to yield blunt-ended nucleic acid fragments; (b) phosphorylating the blunt-ended nucleic acid fragments in a droplet in the oil to yield phosphorylated nucleic acid fragments; coupling A-tails to the phosphorylated nucleic acid fragments in a droplet in the oil to yield A-tailed nucleic acid fragments; and (d) coupling nucleic acid adapters to the A-tailed nucleic acid fragments in a droplet in the oil to yield the nucleic acid library comprising adapter-ligated nucleic acid fragments.

    Abstract translation: 制备与油接触的液滴核酸文库的方法，包括：（a）在油中的液滴中的平端核酸片段以产生平端核酸片段; （b）使油中的液滴中的平端核酸片段磷酸化以产生磷酸化的核酸片段; 将A尾连接到油中的液滴中的磷酸化核酸片段以产生A尾核酸片段; 和（d）将核酸衔接子偶联到油中的液滴中的A尾核酸片段，以产生包含衔接子连接的核酸片段的核酸文库。

    GLUCOSE-6-PHOSPHATE DEHYDROGENASE ASSAYS
    53.
    发明申请
    GLUCOSE-6-PHOSPHATE DEHYDROGENASE ASSAYS 审中-公开
    葡萄糖-6-磷酸脱氢酶测定

    公开(公告)号：US20140335069A1

    公开(公告)日：2014-11-13

    申请号：US14359177

    申请日：2012-11-20

    Applicant: ADVANCED LIQUID LOGIC, INC.

    Inventor： Carrie A. Graham ,  Allen E. Eckhardt

    IPC: C12Q1/32

    CPC classification number: C12Q1/32 ,  G01N2333/904

    Abstract: Aspects of embodiments may include methods for automated enzymatic detection of glucose-6-phosphate dehydrogenase (G6PD) activity. Aspects of embodiments may include methods for enzymatic detection of G6PD activity in droplets in oil. Aspects of embodiments may include a system including a droplet actuator. Aspects of embodiments may include a treatment method.

    Abstract translation: 实施方案的各方面可以包括葡萄糖-6-磷酸脱氢酶（G6PD）活性的自动酶检测方法。 实施方案的各方面可以包括用于在油中的液滴中酶检测G6PD活性的方法。 实施例的方面可以包括包括液滴致动器的系统。 实施方案的各方面可以包括治疗方法。

    Filler Fluids for Droplet Operations
    56.
    发明申请
    Filler Fluids for Droplet Operations 审中-公开
    液滴操作的填充液

    公开(公告)号：US20140231259A1

    公开(公告)日：2014-08-21

    申请号：US14253556

    申请日：2014-04-15

    Applicant: Advanced Liquid Logic, Inc.

    Inventor： Vijay Srinivasan ,  Michael G. Pollack ,  Philip Y. Paik ,  Vamsee K. Pamula

    IPC: G01N27/447

    CPC classification number: B01L3/502792 ,  B01F13/0071 ,  B01F13/0076 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502761 ,  B01L3/502784 ,  B01L7/52 ,  B01L7/525 ,  B01L7/54 ,  B01L2200/0647 ,  B01L2200/0673 ,  B01L2200/10 ,  B01L2200/147 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/089 ,  B01L2300/1827 ,  B01L2400/0427 ,  B01L2400/0448 ,  B03C5/02 ,  B81B1/00 ,  G01N27/447 ,  G01N2035/1046

    Abstract: The present invention relates to filler fluids for droplet operations. According to one embodiment of this aspect, a droplet microactuator including an opaque filler fluid and a transparent droplet thereon is provided. The droplet microactuator may further include a first substrate comprising electrodes configured to effect electrowetting mediated droplet operations on a surface of the first substrate; a second substrate spaced apart from the surface of the first substrate by a distance sufficient to define an interior volume between the first substrate and second substrate; and wherein the droplet and the filler fluid are in the interior volume, and wherein the droplet is surrounded by the filler fluid and arranged with respect to the electrodes in a manner which permits electrowetting mediated droplet operations to be effected on the droplet using the electrodes.

    Abstract translation: 本发明涉及用于液滴操作的填充流体。 根据该方面的一个实施例，提供了包括不透明填充流体和其上的透明液滴的液滴微致动器。 液滴微致动器还可以包括第一衬底，其包括被配置为在第一衬底的表面上实现电润湿介导的液滴操作的电极; 第二基板，与第一基板的表面间隔开足以限定第一基板和第二基板之间的内部空间的距离; 并且其中所述液滴和所述填充流体在所述内部容积中，并且其中所述液滴被所述填充流体包围并且以允许使用所述电极对所述液滴进行电润湿介导的液滴操作的方式相对于所述电极布置。

    Droplet Actuators that Include Molecular Barrier Coatings
    58.
    发明申请
    Droplet Actuators that Include Molecular Barrier Coatings 有权
    包含分子阻隔涂层的滴液致动器

    公开(公告)号：US20130333762A1

    公开(公告)日：2013-12-19

    申请号：US13915842

    申请日：2013-06-12

    Applicant: Advanced Liquid Logic Inc.

    Inventor： Theodore Winger

    IPC: G05D7/06

    CPC classification number: G05D7/0629 ,  B01L3/0268 ,  B01L3/502707 ,  B01L3/502784 ,  B01L7/52 ,  B01L2200/0668 ,  B01L2300/0681 ,  B01L2300/0887 ,  B01L2300/12 ,  B01L2300/16 ,  B01L2300/161 ,  B01L2300/165 ,  B01L2400/0427 ,  B01L2400/043 ,  Y10T137/0318 ,  Y10T137/206

    Abstract: Droplet actuators that include molecular barrier coatings are provided. The molecular barrier coating may be provided atop the conductive layer of the top substrate, atop the droplet operations electrodes of the bottom substrate, or both. Where the conductive layer of the top substrate and/or the droplet operations electrodes of the bottom substrate are formed of an electrically conductive organic polymer, such as poly(3,4-ethylenedioxythiophene) poly(styrenesulfonate) (PEDOT:PSS), the molecular barrier coating helps to immobilize the contents of the PEDOT:PSS layer. Further, the molecular barrier coating reduces, preferably entirely eliminates, moisture from seeping into the electrically conducting organic polymer. Methods of conducting droplet operations using the disclosed droplet actuators are also provided.

    Abstract translation: 提供了包括分子阻隔涂层的液滴致动器。 分子屏障涂层可以设置在顶部基底的导电层顶部，位于底部基底的液滴操作电极顶部，或两者之上。 在顶部基板的导电层和/或底部基板的液滴操作电极由诸如聚（3,4-亚乙基二氧噻吩）聚（苯乙烯磺酸盐）（PEDOT：PSS）的导电有机聚合物形成的情况下，分子 阻隔涂层有助于固定PEDOT：PSS层的内容物。 此外，分子阻隔涂层减少，优选完全消除水分渗透到导电有机聚合物中。 还提供了使用所公开的液滴致动器进行液滴操作的方法。

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