公开(公告)号：US20040229349A1

公开(公告)日：2004-11-18

申请号：US10640510

申请日：2003-08-12

Applicant: Fluidigm Corporation

Inventor： Antoine Daridon

IPC: C12M001/22

CPC classification number: C12M1/34 ,  B01L3/502738 ,  B01L3/502746 ,  B01L3/502753 ,  B01L3/502761 ,  B01L2200/0636 ,  B01L2200/0647 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2200/10 ,  B01L2200/12 ,  B01L2200/16 ,  B01L2300/06 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0681 ,  B01L2300/0861 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2300/088 ,  B01L2300/0887 ,  B01L2300/0893 ,  B01L2300/123 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/0481 ,  B01L2400/0622 ,  B01L2400/0655 ,  C12M21/06 ,  C12M23/16 ,  G01N15/1484 ,  G01N33/4833 ,  G01N2015/008 ,  G01N2015/0288 ,  G01N2015/149 ,  G01N2015/1493 ,  G02B21/32

Abstract: The invention provides systems, including apparatus, methods, and kits, for the microfluidic manipulation and/or detection of particles, such as cells and/or beads. The invention provides systems, including apparatus, methods, and kits, for the microfluidic manipulation and/or analysis of particles, such as cells, viruses, organelles, beads, and/or vesicles. The invention also provides microfluidic mechanisms for carrying out these manipulations and analyses. These mechanisms may enable controlled input, movement/positioning, retention/localization, treatment, measurement, release, and/or output of particles. Furthermore, these mechanisms may be combined in any suitable order and/or employed for any suitable number of times within a system. Accordingly, these combinations may allow particles to be sorted, cultured, mixed, treated, and/or assayed, among others, as single particles, mixed groups of particles, arrays of particles, heterogeneous particle sets, and/or homogeneous particle sets, among others, in series and/or in parallel. In addition, these combinations may enable microfluidic systems to be reused. Furthermore, these combinations may allow the response of particles to treatment to be measured on a shorter time scale than was previously possible. Therefore, systems of the invention may allow a broad range of cell and particle assays, such as drug screens, cell characterizations, research studies, and/or clinical analyses, among others, to be scaled down to microfluidic size. Such scaled-down assays may use less sample and reagent, may be less labor intensive, and/or may be more informative than comparable macrofluidic assays.

公开(公告)号：US20020158022A1

公开(公告)日：2002-10-31

申请号：US10118469

申请日：2002-04-05

Applicant: Fluidigm Corporation

Inventor： Jiang Huang ,  Hou-Pu Chou ,  Marc A. Unger

IPC: B01D015/08

CPC classification number: B01D15/08 ,  G01N30/6065 ,  G01N30/6095 ,  G01N2030/387 ,  G01N2030/521 ,  Y10T137/86863 ,  Y10T436/2575

Abstract: The present invention is directed to a microfluidic chromatography apparatus comprising a microfabricated fluid delivery system and a chromatography column which is in fluid communication with the fluid delivery system, and a method for producing and using the same. Preferably, the chromatography column comprises an OTLC, PCLC, or combinations thereof.

Abstract translation: 本发明涉及包含微流体输送系统和与流体输送系统流体连通的色谱柱的微流体色谱装置及其制造和使用方法。 优选地，色谱柱包括OTLC，PCLC或其组合。

公开(公告)号：US20020108097A1

公开(公告)日：2002-08-08

申请号：US09894858

申请日：2001-06-27

Applicant: Fluidigm Corporation

Inventor： Gregory Harris ,  James Montgomery ,  Michael Lee ,  Gajus Worthington

IPC: G06F017/50

CPC classification number: F16K99/0059 ,  B01L3/502707 ,  B01L2200/0621 ,  B01L2200/12 ,  B01L2200/14 ,  B01L2300/123 ,  B01L2400/0487 ,  B01L2400/0638 ,  B01L2400/0655 ,  F16K99/0001 ,  F16K2099/008 ,  F16K2099/0082 ,  G06F17/509 ,  G06F2217/16 ,  H01H2029/008 ,  Y10S707/99943

Abstract: The present invention provides for the design of a microfluidic system, including a microfluidic chip or circuit, using an object oriented microfluidic computer aided design system. In an embodiment of the present invention, in a computer system having a computer memory and an object-oriented environment, a method for physically laying out a microfluidic circuit, having a plurality of microfluidic components is provided. First, a first symbol object representing a microfluidic component is placed, where the first symbol object includes a fluid channel object which represents a first fluid channel of the microfluidic component. Next, a connecting fluid channel object on a channel layer is placed, where the connecting fluid channel object represents a second fluid channel used to connect two microfluidic components of the plurality of microfluidic components. The fluid channel object is then linked to the connecting fluid channel object, where the linking represents connecting the first fluid channel to the second fluid channel.

Abstract translation: 本发明提供了使用面向对象的微流控计算机辅助设计系统的微流体系统的设计，包括微流体芯片或电路。 在本发明的一个实施例中，在具有计算机存储器和面向对象环境的计算机系统中，提供了一种用于物理布置具有多个微流体部件的微流体电路的方法。 首先，放置表示微流体成分的第一符号对象，其中第一符号对象包括表示微流体成分的第一流体通道的流体通道对象。 接下来，放置通道层上的连接流体通道物体，其中连接流体通道物体表示用于连接多个微流体成分的两个微流体成分的第二流体通道。 然后将流体通道物体连接到连接流体通道对象，其中连接表示将第一流体通道连接到第二流体通道。

公开(公告)号：US11857940B2

公开(公告)日：2024-01-02

申请号：US17398865

申请日：2021-08-10

Applicant: Fluidigm Corporation

Inventor： Peilin Chen

IPC: C12Q1/6853 ,  C12Q1/6848 ,  C12Q1/6844 ,  B01J19/00

CPC classification number: B01J19/0046 ,  C12Q1/6844 ,  C12Q1/6848 ,  C12Q1/6853 ,  B01J2219/00585 ,  B01J2219/00722 ,  C12Q1/6848 ,  C12Q2525/161 ,  C12Q2525/301 ,  C12Q1/6853 ,  C12Q2525/161 ,  C12Q2525/301

Abstract: The present disclosure provides a “looping amplification” method to increase the specificity of nucleic acid amplification. This increased specificity facilitates multiplexing to a much higher degree than was previously possible.

公开(公告)号：US20210324446A1

公开(公告)日：2021-10-21

申请号：US17166992

申请日：2021-02-03

Applicant: Fluidigm Corporation

Inventor： Andrew May ,  Peilin Chen ,  Jun Wang ,  Fiona Kaper ,  Megan Anderson

IPC: C12Q1/6806 ,  C12Q1/686 ,  B01L3/00

Abstract: In certain embodiments, the present invention provides amplification methods in which nucleotide tag(s) and, optionally, a barcode nucleotide sequence are added to target nucleotide sequences. In other embodiments, the present invention provides a microfluidic device that includes a plurality of first input lines and a plurality of second input lines. The microfluidic device also includes a plurality of sets of first chambers and a plurality of sets of second chambers. Each set of first chambers is in fluid communication with one of the plurality of first input lines. Each set of second chambers is in fluid communication with one of the plurality of second input lines. The microfluidic device further includes a plurality of first pump elements in fluid communication with a first portion of the plurality of second input lines and a plurality of second pump elements in fluid communication with a second portion of the plurality of second input lines.

公开(公告)号：US11117113B2

公开(公告)日：2021-09-14

申请号：US15382360

申请日：2016-12-16

Applicant: Fluidigm Corporation

Inventor： Peilin Chen

IPC: C12Q1/6853 ,  C12Q1/6848 ,  C12Q1/6844 ,  B01J19/00

Abstract: The present disclosure provides a “looping amplification” method to increase the specificity of nucleic acid amplification. This increased specificity facilitates multiplexing to a much higher degree than was previously possible.

公开(公告)号：US20190256881A1

公开(公告)日：2019-08-22

申请号：US16256261

申请日：2019-01-24

Applicant: Fluidigm Corporation

Inventor： Peilin Chen ,  Jing Wang ,  Andrew May

IPC: C12P19/34 ,  C12Q1/6844 ,  C12Q1/6848

Abstract: The present invention provides reagents, kits, and methods for single-cell whole genome amplification using Phi 29 DNA polymerase.

公开(公告)号：US10344318B2

公开(公告)日：2019-07-09

申请号：US15590998

申请日：2017-05-09

Applicant: Fluidigm Corporation

Inventor： Andrew May ,  Peilin Chen ,  Jun Wang ,  Fiona Kaper ,  Megan Anderson

IPC: C12Q1/686 ,  C12Q1/6806 ,  B01L3/00 ,  B01L7/00

Abstract: In certain embodiments, the present invention provides amplification methods in which nucleotide tag(s) and, optionally, a barcode nucleotide sequence are added to target nucleotide sequences. In other embodiments, the present invention provides a microfluidic device that includes a plurality of first input lines and a plurality of second input lines. The microfluidic device also includes a plurality of sets of first chambers and a plurality of sets of second chambers. Each set of first chambers is in fluid communication with one of the plurality of first input lines. Each set of second chambers is in fluid communication with one of the plurality of second input lines. The microfluidic device further includes a plurality of first pump elements in fluid communication with a first portion of the plurality of second input lines and a plurality of second pump elements in fluid communication with a second portion of the plurality of second input lines.

公开(公告)号：US20190169676A1

公开(公告)日：2019-06-06

申请号：US16150096

申请日：2018-10-02

Applicant: Fluidigm Corporation

Inventor： Marc Unger ,  Ian D. Manger ,  Michael Lucero ,  Yong Yi ,  Emily Miyashita-Lin ,  Anja Wienecke ,  Geoffrey Facer

IPC: C12Q1/68 ,  B01L3/00 ,  C12Q1/686 ,  G01N27/453

Abstract: A method for carrying out nucleic acid amplification reactions using a microfluidic device is described. Amplification primers and other amplification reagents are deposited at a plurality of reaction sites in the device, a sample solution containing amplifiable polynucleotides is introduced into the reaction sites, and amplification is carried out.

公开(公告)号：US09909179B2

公开(公告)日：2018-03-06

申请号：US14977414

申请日：2015-12-21

Applicant: Fluidigm Corporation

Inventor： Amy Hamilton ,  Min Lin ,  Alain Mir ,  Martin Pieprzyk

IPC: C12Q1/68 ,  C12P19/34

CPC classification number: C12Q1/6881 ,  C12Q1/6844 ,  C12Q1/686 ,  C12Q2531/113 ,  C12Q2531/119 ,  C12Q2531/137 ,  C12Q2565/101 ,  C12Q2600/156 ,  C12Q2600/158 ,  C12Q2600/16 ,  C12Q2600/178

Abstract: The present invention provides methods for analysis of genomic DNA and/or RNA from small samples or even single cells. Methods for analyzing genomic DNA can entail whole genome amplification (WGA), followed by preamplification and amplification of selected target nucleic acids. Methods for analyzing RNA can entail reverse transcription of the desired RNA, followed by preamplification and amplification of selected target nucleic acids.