公开(公告)号：US20060266650A1

公开(公告)日：2006-11-30

申请号：US11440872

申请日：2006-05-25

申请人： Jung-im Han ,  Young-sun Lee ,  Joon-ho Kim

发明人： Jung-im Han ,  Young-sun Lee ,  Joon-ho Kim

IPC分类号： B01D61/42

CPC分类号： B01D61/422 ,  B01D61/46 ,  B01L3/5027 ,  G01N2001/4011

摘要： An apparatus for regulating salt concentration, a lab-on-a-chip including the same and a method of regulating salt concentration using the apparatus are provided. The apparatus includes: a reaction chamber that is defined by a cation exchange membrane and an anion exchange membrane and is selected from the group consisting of a biomolecule extraction chamber, an amplification chamber, a hybridization chamber, and a detection chamber; a first electrode chamber that is defined by the anion exchange membrane and a first electrode and includes an ion exchange medium; and a second electrode chamber that is defined by the cation exchange membrane and a second electrode and includes an ion exchange medium. Even without injecting solutions with different salt concentrations into the reaction chamber by operating pumps and valves for each operation stage, the salt concentration can be reversibly regulated in situ by adjusting the polarity, intensity and application time of a voltage.

摘要翻译： 提供了一种调节盐浓度的装置，包括该盐浓度的实验室，以及使用该装置调节盐浓度的方法。 该装置包括：由阳离子交换膜和阴离子交换膜限定的反应室，其选自生物分子提取室，扩增室，杂交室和检测室; 由阴离子交换膜和第一电极限定并包括离子交换介质的第一电极室; 以及由阳离子交换膜和第二电极限定并包括离子交换介质的第二电极室。 即使在每个操作阶段通过操作泵和阀都不会将具有不同盐浓度的溶液注入反应室中，可以通过调节电压的极性，强度和施加时间来原位调节盐浓度。

公开(公告)号：US20060127887A1

公开(公告)日：2006-06-15

申请号：US11191221

申请日：2005-07-27

申请人： Young-sun Lee ,  Jung-im Han ,  Hyo-yeon Lee ,  Kak Namkoong ,  Kwang-wook Oh

发明人： Young-sun Lee ,  Jung-im Han ,  Hyo-yeon Lee ,  Kak Namkoong ,  Kwang-wook Oh

IPC分类号： C12Q1/70 ,  C12Q1/68 ,  C12N1/20 ,  C12N5/00 ,  C07K1/26

CPC分类号： C12N15/1006

摘要： Provided is a method of isolating and purifying biomolecules using a hydrogel, the method including: bring a sample containing charged biomolecules into contact with a hydrogel to bind the biomolecules to the hydrogel; washing the hydrogel bound with the biomolecules; and eluting the bound biomolecules using an elution solvent. According to the method, the use of a hydrogel with a large surface area reduces the isolation time of biomolecules to 5 min or less, an external device such as an electromagnet is not required, and small-sized systems or LOC can be easily implemented due to applicability to microsystems through a polymer patterning technique.

公开(公告)号：US08224392B2

公开(公告)日：2012-07-17

申请号：US12769422

申请日：2010-04-28

申请人： Jong Hwan Kim ,  Seung Hwan Yoo ,  Doo Hwan Kim ,  Mi Jung Kim ,  Soo Chul Lim ,  Young Sun Lee ,  Eun Sang Lee

发明人： Jong Hwan Kim ,  Seung Hwan Yoo ,  Doo Hwan Kim ,  Mi Jung Kim ,  Soo Chul Lim ,  Young Sun Lee ,  Eun Sang Lee

IPC分类号： H04M1/00 ,  H04B1/38 ,  G06F3/041

CPC分类号： G06F3/041 ,  G06F3/016 ,  G06F3/0428 ,  G06F3/0488 ,  G06F2203/04106

摘要： A method of controlling a mobile terminal, and which includes displaying, on a touch screen display of the mobile terminal, an operation screen corresponding to a current operating mode executing on the mobile terminal; detecting, via a detecting device, a touch input on the touch screen display of the mobile terminal; determining, via a controller on the mobile terminal, a first finger characteristic describing a finger touching the touch screen display and a second finger characteristic describing the finger touching the touch screen display that is different than the first finger characteristic; performing, via the controller, a first operation relevant to the current operating mode based on the determined first finger characteristic; and performing, via the controller, a second operation relevant to the current operating mode based on the determined second finger characteristic.

摘要翻译： 一种控制移动终端的方法，包括在移动终端的触摸屏显示器上显示与在移动终端上执行的当前操作模式相对应的操作画面; 经由检测装置检测所述移动终端的触摸屏显示器上的触摸输入; 通过所述移动终端上的控制器确定描述触摸所述触摸屏显示的手指的第一手指特征和描述所述手指触摸所述触摸屏显示不同于所述第一手指特征的第二手指特征; 基于所确定的第一手指特征，经由所述控制器执行与所述当前操作模式相关的第一操作; 以及基于所确定的第二手指特征，经由所述控制器执行与所述当前操作模式相关的第二操作。

公开(公告)号：US20090170189A1

公开(公告)日：2009-07-02

申请号：US12184317

申请日：2008-08-01

申请人： Chin-sung PARK ,  Kak Namkoong ,  Young-sun Lee

发明人： Chin-sung PARK ,  Kak Namkoong ,  Young-sun Lee

IPC分类号： C12M1/34 ,  C12M1/00 ,  B32B37/06

CPC分类号： C12M23/16 ,  B01L2200/12 ,  B01L2300/0816 ,  B01L2300/1827 ,  B29C65/02 ,  B29C65/08 ,  B29C66/1122 ,  B29C66/30223 ,  B29C66/47 ,  B29C66/53461 ,  B29C66/61 ,  B29C66/71 ,  B29C66/7352 ,  B29C66/73921 ,  B29C66/8322 ,  B29K2023/06 ,  B29K2023/12 ,  B29K2033/12 ,  B29K2067/00 ,  B29K2069/00 ,  B29L2031/756 ,  Y10T156/10 ,  B29K2067/003 ,  B29K2023/38

摘要： Provided are a microfluidic device that performs a biochemical reaction using a small amount of a biochemical fluid and detects the result thereof, and a method of fabricating the same. The microfluidic device includes: a substrate which comprises a chamber that is formed as a concave groove and accommodates a fluid in the bottom surface of the substrate, and is formed of polymer; and a film welded on the bottom surface of the substrate to seal the chamber so that the chamber is not open at the bottom surface of the substrate, and formed of polymer. The method of fabricating a microfluidic device includes: preparing a substrate which comprises a chamber that is formed as a concave groove and accommodates a fluid in the bottom surface of the substrate, and is formed of polymer; and welding a film on a bottom surface of the substrate to seal the chamber so that the chamber is not opened at the bottom surface of the substrate, the film being formed of polymer.

摘要翻译： 提供了使用少量生物化学流体进行生化反应并检测其结果的微流体装置及其制造方法。 微流体装置包括：基板，其包括形成为凹槽并容纳基板的底表面中的流体的腔室，并且由聚合物形成; 以及焊接在基板的底表面上的膜以密封室，使得室不在基板的底表面处开口，并且由聚合物形成。 制造微流体装置的方法包括：制备基板，其包括形成为凹槽的腔室，并且在基底的底表面容纳流体，并且由聚合物形成; 并且在所述基板的底表面上焊接膜以密封所述室，使得所述室在所述基板的底表面处不被打开，所述膜由聚合物形成。

公开(公告)号：US07517690B2

公开(公告)日：2009-04-14

申请号：US11291053

申请日：2005-11-30

申请人： Jung-im Han ,  Young-sun Lee ,  Kak Namkoong ,  Kwang-wook Oh

发明人： Jung-im Han ,  Young-sun Lee ,  Kak Namkoong ,  Kwang-wook Oh

IPC分类号： G01N33/48

CPC分类号： C12N1/06 ,  Y10T436/107497 ,  Y10T436/206664 ,  Y10T436/25 ,  Y10T436/2575

摘要： Provided is a method of lysing a cell or a virus using a free radical. The method includes: applying an electric field to a mixture of a metal ion, a peroxide, and a cell or virus solution to increase the free radical generation, thereby lysing a cell or a virus. In the present method, cell lysis may be efficiently performed using a low electrical energy (several mV to several V). When the present method is applied to a microsystem, cell lysis can occur at a desired time and in a desired space by controlling the electrical energy, thus being suitable to realize a lab-on-a-chip (LOC).

摘要翻译： 提供了使用自由基裂解细胞或病毒的方法。 该方法包括：向金属离子，过氧化物和细胞或病毒溶液的混合物施加电场以增加自由基产生，从而裂解细胞或病毒。 在本方法中，可以使用低电能（几mV至几V）有效地进行细胞裂解。 当本方法应用于微系统时，细胞裂解可以通过控制电能在期望的时间和期望的空间中发生，从而适于实现芯片上的实验室（LOC）。

公开(公告)号：US07442503B2

公开(公告)日：2008-10-28

申请号：US10765366

申请日：2004-01-27

申请人： Sun-hee Kim ,  Soo-suk Lee ,  Geun-bae Lim ,  Young-sun Lee

发明人： Sun-hee Kim ,  Soo-suk Lee ,  Geun-bae Lim ,  Young-sun Lee

IPC分类号： C12Q1/68 ,  C12P19/34

CPC分类号： B01L3/508 ,  B01L7/52 ,  B01L2300/163 ,  B05D1/62 ,  B05D2203/35 ,  C03C17/30

摘要： Provided is a method of treating a surface of a substrate used in a biochemical reaction system, the method including forming a polymer film on the surface by vapor deposition of a compound of formula (1) below and a compound of formula (2) below: (RO)3—Si—(CH2)n1—X   (1) (RO)3—Si—(CH2)n2—(CF2)m—X   (2) wherein R is one of a methyl group and an ethyl group, X is one of a methyl group and a trifluoromethyl group, n1 is an integer from 1 to 3, n2 is an integer from 1 to 10, and m is an integer from 1 to 10.

摘要翻译： 本发明提供一种处理生物化学反应体系中使用的基板的表面的方法，该方法包括通过以下式（1）的化合物和下述式（2）的化合物的气相沉积在表面上形成聚合物膜： （？）（？）（？）？（？）？（？）？（？）？ n1 -X（1）<？in-line-formula description =“In-line Formulas”end =“tail”？> <？in-line-formula description =“In-line Formulas”end =“ （RO）3 - （CH 2）2 - （CF 2）2 - （CH 2） 其中R是甲基和乙基之一，X是一个，其中R是甲基和乙基之一， 的甲基和三氟甲基，n1为1〜3的整数，n2为1〜10的整数，m为1〜10的整数。

公开(公告)号：US20080145957A1

公开(公告)日：2008-06-19

申请号：US11950907

申请日：2007-12-05

申请人： Young-Sun Lee ,  Ho-Seong Kang ,  Jung-Ho Kwak ,  Bong-su Kim ,  Young-Nam Kim ,  Yi-Su Lee

发明人： Young-Sun Lee ,  Ho-Seong Kang ,  Jung-Ho Kwak ,  Bong-su Kim ,  Young-Nam Kim ,  Yi-Su Lee

IPC分类号： H01L21/66 ,  H01L21/67

CPC分类号： H01L21/6838 ,  H01L21/67288 ,  H01L21/67742 ,  H01L22/20

摘要： A wafer transferring robot in semiconductor device fabricating equipment and a method of detecting wafer warpage by using the wafer transferring robot are provided. In the realizing the wafer transferring robot to transfer a wafer, vacuum lines are formed to adsorb a plurality of regions of the wafer. Whether the wafer is warped or not and the extent of warpage are determined in real time, depending on whether the wafer is adsorbed by the vacuum lines. When no warpage occurs on the wafer or when the extent of warpage is slight, the wafer is allowed to be normally processed. When the extent of warpage is too serious to perform a normal process on the wafer, the wafer is previously processed as an error, thereby preventing the waste of time, cost and manpower caused by performing an unnecessary process.

摘要翻译： 提供半导体器件制造设备中的晶片传送机器人以及通过使用晶片传送机器人检测晶片翘曲的方法。 在实现晶片传送机器人传送晶片的过程中，形成真空线以吸附晶片的多个区域。 取决于晶片是否被真空管线吸附，晶片是否翘曲和翘曲程度是实时确定的。 当晶片上没有发生翘曲时或当翘曲程度轻微时，允许晶片被正常处理。 当翘曲程度太严重以致不能在晶片上进行正常处理时，晶片预先作为错误处理，从而防止由于执行不必要的处理而浪费时间，成本和人力。

公开(公告)号：US20070148588A1

公开(公告)日：2007-06-28

申请号：US11613629

申请日：2006-12-20

申请人： Chin-sung PARK ,  Kyu-youn HWANG ,  Jin-tae KIM ,  Young-sun LEE

发明人： Chin-sung PARK ,  Kyu-youn HWANG ,  Jin-tae KIM ,  Young-sun LEE

IPC分类号： G03C1/00

CPC分类号： G03F7/165 ,  B81B2201/058 ,  B81B2203/0338 ,  B81C1/00071 ,  B81C2201/0191 ,  B82Y30/00 ,  G03F7/11 ,  G03F7/161 ,  G03F7/40

摘要： Disclosed herein is a method of releasing a photoresist film from a substrate, which includes forming a self-assembled monolayer (SAM) on a substrate; coating the SAM with a photoresist film; and rinsing the substrate with an alcohol or an acid. According to the photoresist film releasing method, a photoresist film can be easily released from a substrate without damage after patterning. A method of bonding a released photoresist film with a substrate includes arraying a second substrate and the photoresist film released from a first substrate, and baking the second substrate. According to the bonding method, the photoresist film can be perfectly bonded with a second substrate without generating a crevice even though an additional adhesive is not used.

摘要翻译： 本文公开了一种从衬底上释放光致抗蚀剂膜的方法，其包括在衬底上形成自组装单层（SAM）; 用光致抗蚀剂膜涂覆SAM; 并用醇或酸冲洗底物。 根据光致抗蚀剂膜的释放方法，在图案化之后，光致抗蚀剂膜可以容易地从衬底上释放而不损坏。 将释放的光致抗蚀剂膜与基板接合的方法包括排列第二基板和从第一基板释放的光致抗蚀剂膜，并烘烤第二基板。 根据接合方法，即使没有使用另外的粘合剂，也可以使光致抗蚀剂膜与第二基板完全粘合而不产生缝隙。

公开(公告)号：US20070105215A1

公开(公告)日：2007-05-10

申请号：US11556392

申请日：2006-11-03

申请人： Young Sun LEE ,  Byung KIM ,  Ok SONG

发明人： Young Sun LEE ,  Byung KIM ,  Ok SONG

IPC分类号： C12N5/06 ,  C12N5/08

CPC分类号： C12N5/0693

摘要： Disclosed herein is a method of culturing stem cells, which can remove differentiated cells generated in stem cell culture, using ABC transporters, according to a stem cell's characteristic in that the stem cells contain a plurality of the ABC transporters. The method comprises the steps of: bringing a stem cell culture into contact with an antitumor drug or toxin as a substrate of ATP-Binding Cassette transporters (ABC transporters) to allow the substrate to react with the stem cell culture; and reculturing viable cells among the stem cell culture which reacted with the substrate, the viable cells having no substrate introduced therein. According to the disclosed method, the differentiated cells generated in stem cell culture can be easily removed using an antitumor drug or toxin among various substrates of the ABC transporters. Thus, the method has an excellent effect capable of significantly increasing the purity of a stem cell culture.

摘要翻译： 本文公开了根据干细胞特征的培养干细胞的方法，其可以使用ABC转运蛋白去除在干细胞培养物中产生的分化细胞，因为干细胞含有多个ABC转运蛋白。 该方法包括以下步骤：使干细胞培养物与作为ATP结合盒转运蛋白（ABC转运蛋白）底物的抗肿瘤药物或毒素接触，以使底物与干细胞培养物反应; 并在与底物反应的干细胞培养物中再培养活细胞，活细胞没有引入底物。 根据所公开的方法，可以使用ABC转运蛋白的各种底物中的抗肿瘤药物或毒素容易地除去在干细胞培养物中产生的分化细胞。 因此，该方法具有能够显着提高干细胞培养物的纯度的优异效果。

公开(公告)号：US20060115902A1

公开(公告)日：2006-06-01

申请号：US11291053

申请日：2005-11-30

申请人： Jung-im Han ,  Young-sun Lee ,  Kak Namkoong ,  Kwang-wook Oh

发明人： Jung-im Han ,  Young-sun Lee ,  Kak Namkoong ,  Kwang-wook Oh

IPC分类号： G01N1/10

CPC分类号： C12N1/06 ,  Y10T436/107497 ,  Y10T436/206664 ,  Y10T436/25 ,  Y10T436/2575

摘要： Provided is a method of lysing a cell or a virus using a free radical. The method includes: applying an electric field to a mixture of a metal ion, a peroxide, and a cell or virus solution to increase the free radical generation, thereby lysing a cell or a virus. In the present method, cell lysis may be efficiently performed using a low electrical energy (several mV to several V). When the present method is applied to a microsystem, cell lysis can occur at a desired time and in a desired space by controlling the electrical energy, thus being suitable to realize a lab-on-a-chip (LOC).